BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0600.0010.p (311 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3PQL0.1 B3PQL0_RHIE6 Probable restriction endonuclease protein 288 3e-76 B1ZMM1.1 B1ZMM1_OPITP Restriction endonuclease 263 2e-68 C1A4Q9.1 C1A4Q9_GEMAT Putative restriction endonuclease 261 7e-68 Q1D0F6.1 Q1D0F6_MYXXD Putative type II restriction endonuclease 257 1e-66 Q1IF77.1 Q1IF77_PSEE4 Putative uncharacterized protein 253 1e-65 >B3PQL0.1 B3PQL0_RHIE6 Probable restriction endonuclease protein Length = 333 Score = 288 bits (738), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 140/303 (46%), Positives = 193/303 (63%), Gaps = 9/303 (2%) Query: 1 MKSMWLVRAGERAYLIDEFKEKQAVAIGWAQLGDIGAVNTLQEMKRLLKVTYPGYKEGKI 60 M W+VRA L D FK+K AVAIGW ++GD+ AV T + M L+ +P K + Sbjct: 1 MSKSWMVRAERDGRLFDAFKDKSAVAIGWNEVGDLSAVKTRKAMADLVSGAWPETKPQSV 60 Query: 61 NITAGQIFRFVSEFKIDDQVITYNPEERLYYIGRIKSNYEFKPNYITDKPHIRRIDWEGT 120 I AGQ+ RFV+E ++ D V+TYNP R+Y +G I Y + + + +R + W G Sbjct: 61 AIAAGQLHRFVNEIEVGDMVVTYNPSRRVYLVGEIVGPYRYDKSIDPEDAQVRSVSWSGE 120 Query: 121 VSRDVLTASARNTLGSIMTIISIPNDVQKELLKQMVTGQTAGNQPSQPIESVHXXXXXXX 180 VSRD+L+ +RNTLG+I T+ I N+V EL K M +GQ +P P+ + Sbjct: 121 VSRDLLSVESRNTLGAISTLFRISNEVAAELRKAMTSGQPV--KPDLPLIATDAAEDD-- 176 Query: 181 XXXXXIKEDVIEKSNEFIKDKIAKLDWKQMQKFVAGILRGMGYKTRISPDGPDRGRDIFA 240 + + + ++ EFIKDK+ L W MQ+ VAG+LR +GYKTR+S GPDRG+DI A Sbjct: 177 -----LFKSMENRAREFIKDKVNALAWDDMQELVAGLLRSLGYKTRVSEAGPDRGKDIVA 231 Query: 241 SPDGLGLEEPRIIVEVKHRSGSMGSNQIRSFTGGLRVGDRGIYVSTGGFTKEAKYEADRS 300 SPDG G E PRI+VEVKHR G+MG+ +RSF GG D+G+YVSTGGF++EA+YEA+R+ Sbjct: 232 SPDGFGFESPRIVVEVKHRRGTMGAPDVRSFLGGRHPQDKGLYVSTGGFSREARYEAERA 291 Query: 301 NIP 303 NIP Sbjct: 292 NIP 294 >B1ZMM1.1 B1ZMM1_OPITP Restriction endonuclease Length = 333 Score = 263 bits (671), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 136/303 (44%), Positives = 188/303 (62%), Gaps = 12/303 (3%) Query: 2 KSMWLVRAGERAYLIDEFKEKQAVAIGWAQLGDIGAVNTLQEMKRLLKVTYPGYKEGKIN 61 K+ W++RAGE YLI+EF K V +GW ++G+I + RL V YPG K Sbjct: 3 KNAWMIRAGEGGYLIEEFA-KGLVTVGWHEVGEIKDTSQRDLWLRLATV-YPGSKPATNQ 60 Query: 62 ITAGQIFRFVSEFKIDDQVITYNPEERLYYIGRIKSNYEFKPNYITDKPHIRRIDWEGTV 121 A ++RF K+ D V+TY+P +R Y +G + S+Y ++P + PH R + WE V Sbjct: 61 NAASVLWRFARVVKVGDAVVTYDPTKREYLLGVVTSDYGYEPKRSKEHPHTREVTWEARV 120 Query: 122 SRDVLTASARNTLGSIMTIISIPNDVQKELLKQMVTGQTAGNQPSQPIESVHXXXXXXXX 181 SRD L+ + RN+LGS + + ++P+ EL + +TA +P+ IE Sbjct: 121 SRDALSVATRNSLGSTLALFAVPDAAFTELQAASESKKTA--EPTSGIEDRKEQLVQS-- 176 Query: 182 XXXXIKEDVIEKSNEFIKDKIAKLDWKQMQKFVAGILRGMGYKTRISPDGPDRGRDIFAS 241 D E+S E I+D+I LD +MQ+ +A +LR MGY+TR+SP G DRG D+ AS Sbjct: 177 -----NRDAEEQSRELIEDRILALDPFEMQELIAAVLRAMGYRTRVSPPGSDRGIDVLAS 231 Query: 242 PDGLGLEEPRIIVEVKHRSGS-MGSNQIRSFTGGLRVGDRGIYVSTGGFTKEAKYEADRS 300 PDGLGL+EPRI VEVKHR G+ MGS ++RSF G LR GDRG+YVSTG F+KEAKYEA+R+ Sbjct: 232 PDGLGLQEPRIKVEVKHRPGTAMGSQEVRSFIGSLRSGDRGLYVSTGAFSKEAKYEAERA 291 Query: 301 NIP 303 IP Sbjct: 292 TIP 294 >C1A4Q9.1 C1A4Q9_GEMAT Putative restriction endonuclease Length = 326 Score = 261 bits (666), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 125/305 (40%), Positives = 195/305 (63%), Gaps = 15/305 (4%) Query: 1 MKSMWLVRAGERAYLIDEFKEKQAVAIGWAQLGDIGAVNTLQEMKRLLKVTYPGYKEGKI 60 M W+VRAGE YL EF EK+ VA+G+ +G + TL K L YP GK Sbjct: 1 MPGFWMVRAGEGGYLAKEFVEKECVAVGFDGVGSFEQLTTLGAFKGRLLGVYPDDSPGKR 60 Query: 61 NITAGQIFRFVSEFKIDDQVITYNPEERLYYIGRIKSNYEFKPNYITDKPHIRRIDWEGT 120 AG +F + + D+++TY+P+ RLY++GR++ +YE++P +++D H+RR+ W+ Sbjct: 61 AGAAGVGHKFRTLVQRGDRILTYDPDTRLYHLGRVEGDYEYRPGFVSDYSHVRRVKWDRQ 120 Query: 121 VSRDVLTASARNTLGSIMTIISIPNDVQKELLKQMVTGQTAG--NQPSQPIESVHXXXXX 178 + RD L++ RNTLGS +T+ + + +E+L+ V+ +T ++ + E+ Sbjct: 121 ILRDTLSSGVRNTLGSTLTLFEPGDAILQEMLRSGVSAETPAVLDEEDRGDETT------ 174 Query: 179 XXXXXXXIKEDVIEKSNEFIKDKIAKLDWKQMQKFVAGILRGMGYKTRISPDGPDRGRDI 238 I + + +S+EFI D++ +L +M++ +A +LR MG+K R++ GPDRGRD+ Sbjct: 175 -------IYLEQLSRSHEFIADRVNRLSPHEMEQLLAALLRTMGFKARVTLPGPDRGRDV 227 Query: 239 FASPDGLGLEEPRIIVEVKHRSGSMGSNQIRSFTGGLRVGDRGIYVSTGGFTKEAKYEAD 298 ASPDGLGL+ PRI EVKHR GS+G+ ++RSFT LR DRG+++STGGFT+EAKYE D Sbjct: 228 VASPDGLGLQSPRIFCEVKHRKGSIGAPEVRSFTSTLRGDDRGLFLSTGGFTREAKYEGD 287 Query: 299 RSNIP 303 RS++P Sbjct: 288 RSHVP 292 >Q1D0F6.1 Q1D0F6_MYXXD Putative type II restriction endonuclease Length = 340 Score = 257 bits (656), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 134/307 (43%), Positives = 187/307 (60%), Gaps = 15/307 (4%) Query: 2 KSMWLVRAGERAYLIDEFKEKQAVAIGWAQLGDIGAVNTLQEMKRLLKVTYPGYKEGKIN 61 K +W +RAG A D FK + V IGW ++G + + E++ YP KEG Sbjct: 6 KRLWFIRAGRDAAFFDAFKAEGHVGIGWDKVGPLAPDLSADELEARFAKAYPEDKEGTRR 65 Query: 62 ITAGQIFRFVSEFKIDDQVITYNPEERLYYIGRIKSNYEFKPNYITDKPHIRRIDWEGTV 121 + A Q+ RF+ + + D +TY+P ER+Y +GRI+S E N P +RR+ W V Sbjct: 66 VWAAQVRRFMRDVEQGDSAVTYDPNERVYLLGRIESGVE---NRAGPPPLVRRVRWTHHV 122 Query: 122 SRDVLTASARNTLGSIMTIISIPNDVQKELLKQMVTGQ----TAGNQPSQPIESVHXXXX 177 R+ L+A+ RN LGSI T+ + EL Q V + + P+ ++S Sbjct: 123 LRNQLSAATRNGLGSIATLFRPSAEATAELWAQAVELERPVASLAPTPAPAVDS------ 176 Query: 178 XXXXXXXXIKEDVIEKSNEFIKDKIAKLDWKQMQKFVAGILRGMGYKTRISPDGPDRGRD 237 ++E+ EK+ +FI+D+IA+L+W +MQ+ VAGILR MGY+TR++ G DRG D Sbjct: 177 -DEGTEAVLREEQFEKAEQFIEDRIARLNWSEMQELVAGILRAMGYRTRVAAPGADRGVD 235 Query: 238 IFASPDGLGLEEPRIIVEVKHRSG-SMGSNQIRSFTGGLRVGDRGIYVSTGGFTKEAKYE 296 IFASPDGLGL+EPRI EVKHR G SM S IR+F GG + GDR +YVSTGGFTK+A+YE Sbjct: 236 IFASPDGLGLQEPRIFCEVKHRVGESMNSQSIRAFLGGRKQGDRCLYVSTGGFTKDARYE 295 Query: 297 ADRSNIP 303 ADRS++P Sbjct: 296 ADRSSVP 302 >Q1IF77.1 Q1IF77_PSEE4 Putative uncharacterized protein Length = 333 Score = 253 bits (646), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 139/309 (44%), Positives = 186/309 (60%), Gaps = 21/309 (6%) Query: 1 MKSMWLVRAGERAYLIDEFKEKQAVAIGWAQLG-DIGAVNTLQEMKRLLKVTYPGYKEGK 59 M +MW++R G+ L D+F+++ AIGWAQL + + + + + K PG K+ Sbjct: 1 MPAMWMIR-GDGGRLYDDFRDRSLAAIGWAQLALEAKPGVSRKALIQAYKDLQPGIKDAS 59 Query: 60 INITAGQIFRFVSEFKIDDQVITYNPEERLYYIGRIKSNYEFKPNYITDKPHIRR-IDW- 117 A Q+FRFV+E I D V+TY+P R Y +GR +P+ D + R ++W Sbjct: 60 AVAGASQVFRFVNEVNIGDTVVTYSPANRTYLVGRFTGTCLLRPDLADDGMSLTRPVEWY 119 Query: 118 EGTVSRDVLTASARNTLGSIMTIISIPNDVQKELLKQMVTGQTAGNQPSQPIESVHXXXX 177 V RD LT ++RN+LGS +T+ + D QKELL + TG++ P IE Sbjct: 120 TQEVDRDKLTGASRNSLGSTLTVFKVSEDAQKELLA-LATGKSVSRPPKDLIED------ 172 Query: 178 XXXXXXXXIKED----VIEKSNEFIKDKIAKLDWKQMQKFVAGILRGMGYKTRISPDGPD 233 + ED V E + E IKD+I LDW +MQ+ VAGILR MGYKT +SP G D Sbjct: 173 ------SDVLEDPLKGVQEIAFERIKDQINSLDWAEMQELVAGILRAMGYKTLVSPAGAD 226 Query: 234 RGRDIFASPDGLGLEEPRIIVEVKHRSGSMGSNQIRSFTGGLRVGDRGIYVSTGGFTKEA 293 RG+DI ASPDG G E PRI+VEVKHR+ MGSN+IRSF GG DRG+YVSTGGFTKEA Sbjct: 227 RGKDIIASPDGFGFEPPRIVVEVKHRNARMGSNEIRSFLGGRHKDDRGLYVSTGGFTKEA 286 Query: 294 KYEADRSNI 302 YE +R+N+ Sbjct: 287 LYEGERANV 295 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,775,968,103 Number of extensions: 73054139 Number of successful extensions: 159257 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 159207 Number of HSP's successfully gapped: 62 Length of query: 311 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 176 Effective length of database: 1,788,859,178 Effective search space: 314839215328 Effective search space used: 314839215328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)