BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0144.p (410 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7Z177.1 A7Z177_BACA2 NasA 350 2e-94 Q5WHJ5.1 Q5WHJ5_BACSK Nitrate/nitrite transporter 341 7e-92 Q65NP1.1 Q65NP1_BACLD Nitrate transporter 341 7e-92 P42432.2 NASA_BACSU Nitrate transporter 337 1e-90 Q5KYT6.1 Q5KYT6_GEOKA Nitrate/nitrite transporter 332 3e-89 >A7Z177.1 A7Z177_BACA2 NasA Length = 401 Score = 350 bits (897), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 189/409 (46%), Positives = 253/409 (61%), Gaps = 9/409 (2%) Query: 1 MEAKGFRKAGHAPSLLSAFLYFDVSFMIWVLCGALSLYITKDFGLSDTQKAAMVAIPILG 60 M+ + +GH +LL +FLYFDVSFMIWV+ GAL +YI++DFGLS +K +VA+PIL Sbjct: 1 MKLSELKTSGHPLTLLCSFLYFDVSFMIWVMLGALGVYISQDFGLSSFEKGFVVALPILS 60 Query: 61 GSIFRIPMGILADRIGSKKAGLLGMFLTIIPXXXXXXXXTSLLQMHMIGFLLGFAGASFA 120 GS+FRI +G+L DRIG KK ++GM +T+IP SL ++ IG LLG AGASFA Sbjct: 61 GSVFRIILGVLTDRIGPKKTAVIGMLITMIPLLWGAFGGRSLTELFAIGILLGVAGASFA 120 Query: 121 VSLSLASRWYPPQYQGLAMGIAGAGNSGTALATFFGPQIAQAYGWHSVFGIALIPLVLVM 180 V+L +ASRWYPP QGLAMGIAGAGNSGT AT FGP++A+ +GWHSV GIAL PL++V Sbjct: 121 VALPMASRWYPPHLQGLAMGIAGAGNSGTLFATLFGPRLAEQFGWHSVMGIALFPLMIVF 180 Query: 181 IVFXXXXXXXXXXXXXXXXXSYLSVFKHADTWWFSMFYAITFGGFVGFANYASIFFYDVY 240 I+F +YL VF+ +TW F + Y +TFGGFVG +++ +IFF D Y Sbjct: 181 ILFIVMAKDSPAQPAPQPLKNYLHVFRQKETWCFCLLYGVTFGGFVGLSSFLAIFFVDQY 240 Query: 241 GDGVHSGGLTKVQVGYLVTITVIAGSFFRPLGGWIADRIGGMRLLLVLYAVVSVCAFAIA 300 L+K+ G VTI V AGSFFRP GGWI+DR GG ++L VL+ + ++C ++ Sbjct: 241 -------QLSKIHAGDFVTICVAAGSFFRPAGGWISDRAGGAKVLAVLFVIAALCMAGVS 293 Query: 301 SMPSSFLVMLLYTSVMMACLGMGNGSVFQIIPQRFSSEIXXXXXXXXXXXXXXXXXXXXX 360 S+P V++L MMA LGMGNG+VFQ++PQ F+ EI Sbjct: 294 SLPPLSTVIVLLFIGMMA-LGMGNGAVFQLVPQHFAKEI-GMVTGIVGAAGGIGGFFLPN 351 Query: 361 XXXXXKQSTGSQVTGFLVVGSIVLLTTLIFYAVTRSWKKSWAKAESGVN 409 KQ TG+ GF+ I LL ++ A W+KS+ S + Sbjct: 352 ILGSLKQMTGTYAIGFISFSCIALLAFVLVLAAGYFWRKSYGTENSPAD 400 >Q5WHJ5.1 Q5WHJ5_BACSK Nitrate/nitrite transporter Length = 401 Score = 341 bits (875), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 192/408 (47%), Positives = 252/408 (61%), Gaps = 13/408 (3%) Query: 1 MEAKGFRKAGHAPSLLSAFLYFDVSFMIWVLCGALSLYITKDFGLSDTQKAAMVAIPILG 60 M+ RK+GHAPSL SAFLYFDVSFMIWVL GAL YIT DFGLS +Q +V IPIL Sbjct: 1 MKLSELRKSGHAPSLFSAFLYFDVSFMIWVLLGALGAYITADFGLSASQIGFIVGIPILA 60 Query: 61 GSIFRIPMGILADRIGSKKAGLLGMFLTIIPXXXXXXXXTSLLQMHMIGFLLGFAGASFA 120 GS+FRI +GIL DR G KK + GM +T++P TSL ++ IG LLG AGASF+ Sbjct: 61 GSVFRILVGILTDRYGPKKTSIAGMVVTMVPLLWGWLFGTSLSELIAIGLLLGVAGASFS 120 Query: 121 VSLSLASRWYPPQYQGLAMGIAGAGNSGTALATFFGPQIAQAYGWHSVFGIALIPLVLVM 180 SL +ASRWYPP QG+AMGIAGAGNSGT AT FGP++A+ GW+ V G+ALIPL LV+ Sbjct: 121 ASLPMASRWYPPHLQGVAMGIAGAGNSGTLFATLFGPRLAEIVGWNGVMGLALIPLSLVL 180 Query: 181 IVFXXXXXXXXXXXXXXXXXSYLSVFKHADTWWFSMFYAITFGGFVGFANYASIFFYDVY 240 I + +Y VF+ DTW+F + Y++TFGGFVG +++ S FF + Y Sbjct: 181 ISYILMAKEPPNQPAPKPLKAYFRVFQERDTWYFCLLYSVTFGGFVGLSSFLSYFFVNQY 240 Query: 241 GDGVHSGGLTKVQVGYLVTITVIAGSFFRPLGGWIADRIGGMRLLLVLYAVVSVCAFAIA 300 ++ V+ G VT+ V AGS RP+GG IAD+IGG RLL VL+ V++C FA++ Sbjct: 241 -------AVSGVRAGEFVTLVVAAGSLLRPIGGLIADKIGGARLLTVLFVGVALCMFAVS 293 Query: 301 SMPS-SFLVMLLYTSVMMACLGMGNGSVFQIIPQRFSSEIXXXXXXXXXXXXXXXXXXXX 359 +P +F + +L+ + M CLGMGNG+VFQ++PQRF EI Sbjct: 294 FLPPIAFALTVLF--IGMGCLGMGNGAVFQLVPQRFHKEI-GMVTGIVGAAGGVGGFFLP 350 Query: 360 XXXXXXKQSTGSQVTGFLVVG-SIVLLTTLIFYAVTRSWKKSWAKAES 406 K TG+ +GFL+ S L+ +A SWKK+W + + Sbjct: 351 NILGLFKDITGTYASGFLLFALSACFALGLLLFA-QHSWKKAWTETKQ 397 >Q65NP1.1 Q65NP1_BACLD Nitrate transporter Length = 401 Score = 341 bits (875), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 188/409 (45%), Positives = 244/409 (59%), Gaps = 9/409 (2%) Query: 1 MEAKGFRKAGHAPSLLSAFLYFDVSFMIWVLCGALSLYITKDFGLSDTQKAAMVAIPILG 60 M+ RK+GHAPSLLS+FLYFDVSFMIWVL GAL +Y+T DF LS +Q +VAIP L Sbjct: 1 MKLADLRKSGHAPSLLSSFLYFDVSFMIWVLLGALGVYVTADFDLSPSQVGLIVAIPTLA 60 Query: 61 GSIFRIPMGILADRIGSKKAGLLGMFLTIIPXXXXXXXXTSLLQMHMIGFLLGFAGASFA 120 GS FRI +GIL DR G KK + GM +T+IP +S+ ++++IG LLG AGASF+ Sbjct: 61 GSFFRIALGILTDRFGPKKTAVGGMLVTMIPLLWGWQFGSSVAELYIIGILLGVAGASFS 120 Query: 121 VSLSLASRWYPPQYQGLAMGIAGAGNSGTALATFFGPQIAQAYGWHSVFGIALIPLVLVM 180 SL +ASRWYPP QGLAMGIAGAGNSGT LAT FGP++A+ GW+ V G+ALIPL LV+ Sbjct: 121 ASLPMASRWYPPHLQGLAMGIAGAGNSGTLLATLFGPRLAEQVGWNGVMGLALIPLTLVL 180 Query: 181 IVFXXXXXXXXXXXXXXXXXSYLSVFKHADTWWFSMFYAITFGGFVGFANYASIFFYDVY 240 + Y +VFK DTW+F + Y++TFGGFVG +N+ S FF Y Sbjct: 181 FSYFLMAKEPPNQPAPKPLKDYFAVFKERDTWYFCLLYSVTFGGFVGLSNFLSYFFASQY 240 Query: 241 GDGVHSGGLTKVQVGYLVTITVIAGSFFRPLGGWIADRIGGMRLLLVLYAVVSVCAFAIA 300 + V+ G VT+ V +GS RP+GG IAD+IGG+RLL L+ V++C F+++ Sbjct: 241 -------AVPAVRAGEFVTLVVASGSCLRPVGGLIADKIGGVRLLQFLFIGVALCMFSVS 293 Query: 301 SMPSSFLVMLLYTSVMMACLGMGNGSVFQIIPQRFSSEIXXXXXXXXXXXXXXXXXXXXX 360 +P S V+ + M CLGMGNG++FQ +PQRF EI Sbjct: 294 MLP-SLGVVTAALFIGMGCLGMGNGAIFQFVPQRFQQEI-GMVTGIVGAAGGIGGFFLPN 351 Query: 361 XXXXXKQSTGSQVTGFLVVGSIVLLTTLIFYAVTRSWKKSWAKAESGVN 409 KQ TG+ +GFL I L + WKK W E + Sbjct: 352 ILGTLKQITGTYSSGFLTFTVIAFLALGLLLFAQAGWKKQWKAEEKSIR 400 >P42432.2 NASA_BACSU Nitrate transporter Length = 401 Score = 337 bits (864), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 194/410 (47%), Positives = 260/410 (63%), Gaps = 11/410 (2%) Query: 1 MEAKGFRKAGHAPSLLSAFLYFDVSFMIWVLCGALSLYITKDFGLSDTQKAAMVAIPILG 60 M+ + +GH +LL +FLYFDVSFMIWV+ GAL +YI++DFGLS +K +VA+PIL Sbjct: 1 MKLSELKTSGHPLTLLCSFLYFDVSFMIWVMLGALGVYISQDFGLSPFEKGLVVAVPILS 60 Query: 61 GSIFRIPMGILADRIGSKKAGLLGMFLTIIPXXXXXXXXTSLLQMHMIGFLLGFAGASFA 120 GS+FRI +GIL DRIG KK ++GM +T+IP SL +++ IG LLG AGASFA Sbjct: 61 GSVFRIILGILTDRIGPKKTAVIGMLVTMIPLLWGTFGGRSLTELYAIGILLGVAGASFA 120 Query: 121 VSLSLASRWYPPQYQGLAMGIAGAGNSGTALATFFGPQIAQAYGWHSVFGIALIPLVLVM 180 V+L +ASRWYPP QGLAMGIAGAGNSGT AT FGP++A+ +GWH V GIALIPL++V Sbjct: 121 VALPMASRWYPPHLQGLAMGIAGAGNSGTLFATLFGPRLAEQFGWHIVMGIALIPLLIVF 180 Query: 181 IVFXXXXXXXXXXXXXXXXXSYLSVFKHADTWWFSMFYAITFGGFVGFANYASIFFYDVY 240 I+F SYL VF +TW+F + Y++TFGGFVG +++ SIFF D Y Sbjct: 181 ILFVSMAKDSPAQPSPQPLKSYLHVFGQKETWFFCLLYSVTFGGFVGLSSFLSIFFVDQY 240 Query: 241 GDGVHSGGLTKVQVGYLVTITVIAGSFFRPLGGWIADRIGGMRLLLVLYAVVSVCAFAIA 300 L+K+ G VT+ V AGSFFRP+GG I+DR+GG ++L VL+ +V++C ++ Sbjct: 241 -------QLSKIHAGDFVTLCVAAGSFFRPVGGLISDRVGGTKVLSVLFVIVALCMAGVS 293 Query: 301 SMPS-SFLVMLLYTSVMMACLGMGNGSVFQIIPQRFSSEIXXXXXXXXXXXXXXXXXXXX 359 S+PS S +++LL+ V M LGMGNG+VFQ++PQRF EI Sbjct: 294 SLPSLSMVIVLLF--VGMMGLGMGNGAVFQLVPQRFRKEI-GMVTGIVGAAGGIGGFFLP 350 Query: 360 XXXXXXKQSTGSQVTGFLVVGSIVLLTTLIFYAVTRSWKKSWAKAESGVN 409 KQ TG+ GF+ I LL + A W+KSW+ S + Sbjct: 351 NILGSLKQMTGTYAIGFITFSCIALLAFALVLAAGYYWRKSWSAESSPAD 400 >Q5KYT6.1 Q5KYT6_GEOKA Nitrate/nitrite transporter Length = 400 Score = 332 bits (852), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 171/339 (50%), Positives = 227/339 (66%), Gaps = 8/339 (2%) Query: 1 MEAKGFRKAGHAPSLLSAFLYFDVSFMIWVLCGALSLYITKDFGLSDTQKAAMVAIPILG 60 M K F + GHAP+LLS+FLYFD+SFMIW L GAL +++ + F L+ +K MVAIP+LG Sbjct: 1 MARKSFWRVGHAPTLLSSFLYFDISFMIWNLLGALGVFVAESFALTPAEKGMMVAIPVLG 60 Query: 61 GSIFRIPMGILADRIGSKKAGLLGMFLTIIPXXXXXXXXTSLLQMHMIGFLLGFAGASFA 120 G++ RIPMG+LADR G K+AGLLGM +T +P + ++ +GFLLG GASFA Sbjct: 61 GALLRIPMGMLADRWGGKRAGLLGMAVTAVPLVWGWQFADRMSDVYALGFLLGVGGASFA 120 Query: 121 VSLSLASRWYPPQYQGLAMGIAGAGNSGTALATFFGPQIAQAYGWHSVFGIALIPLVLVM 180 V+L LASRWYP +YQGLAMGIAGAGNSGT LAT FGP++A+AYGW++VFG+AL+PLV+V Sbjct: 121 VALPLASRWYPKEYQGLAMGIAGAGNSGTVLATLFGPRLAEAYGWNAVFGLALVPLVVVF 180 Query: 181 IVFXXXXXXXXXXXXXXXXXSYLSVFKHADTWWFSMFYAITFGGFVGFANYASIFFYDVY 240 +F Y +V + + W F FY+++FGGFVG Y IFF+D Y Sbjct: 181 FLFAWLARECPTAVKPVSLREYGAVLRRKEAWLFCFFYSLSFGGFVGLTGYLGIFFFDEY 240 Query: 241 GDGVHSGGLTKVQVGYLVTITVIAGSFFRPLGGWIADRIGGMRLLLVLYAVVSVCAFAIA 300 G++KV G VT V AGSF RP+GG++ADRIGGMRLL +L+AV +V I Sbjct: 241 -------GVSKVTAGDFVTAVVFAGSFIRPIGGYLADRIGGMRLLSLLFAVGAVLLAFIG 293 Query: 301 SMPSSFLVMLLYTSVMMACLGMGNGSVFQIIPQRFSSEI 339 ++P +V L V+ C G NG++FQ++P F E+ Sbjct: 294 ALPPLLIVFGLMV-VLFLCFGAANGALFQVVPTWFPKEV 331 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,059,147,451 Number of extensions: 75393570 Number of successful extensions: 320477 Number of sequences better than 1.0e-05: 1049 Number of HSP's gapped: 319131 Number of HSP's successfully gapped: 1121 Length of query: 410 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 272 Effective length of database: 1,760,596,133 Effective search space: 478882148176 Effective search space used: 478882148176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)