BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0138.p (308 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8YHH6.1 A8YHH6_MICAE Genome sequencing data, contig C313 272 3e-71 Q8XWZ7.1 Q8XWZ7_RALSO Probable short-chain dehydrogenase/reducta... 258 4e-67 B1FBK5.1 B1FBK5_9BURK Short-chain dehydrogenase/reductase SDR 257 9e-67 Q0B2G1.1 Q0B2G1_BURCM Short-chain dehydrogenase/reductase SDR 256 2e-66 B5SDA3.1 B5SDA3_RALSO Short-chain dehydrogenase/reductase sdr; p... 247 8e-64 >A8YHH6.1 A8YHH6_MICAE Genome sequencing data, contig C313 Length = 265 Score = 272 bits (696), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 134/261 (51%), Positives = 189/261 (72%), Gaps = 5/261 (1%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 N +GK +LITGASSGIGE FAR+LAK G +L+LVAR++ KL T+A +L + YG++ +V+ Sbjct: 3 NYRGKTALITGASSGIGEIFARELAKRGMNLILVARTEEKLRTIATDLYQVYGVRTEVIV 62 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS E AA +++ ++ L +DLLVNNAGF + FEQI E+ +VM+NV A+VD+ Sbjct: 63 VDLSRENAAQDVFSAVEEKALTVDLLVNNAGFLNYAPFEQIPLEQDRAQVMVNVVALVDL 122 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 TH F+PGML +G GAVIN+SS+ FQP+PYMAVYGA+KAFVLSF++ALW E R RG++ Sbjct: 123 THKFIPGMLAKGEGAVINLSSSGAFQPMPYMAVYGASKAFVLSFSEALWAEYRHRGLRVL 182 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNY-LGAQIS 287 ALCPG T T ++V D G+ +P+RVV V L+A++ G+ YV+PGL+NY L I Sbjct: 183 ALCPGPTATR--SLVKIFDD--GSATSPQRVVAVALKALEDGRNYVIPGLKNYWLANSIP 238 Query: 288 RFMTRRQSLRLAQGMLSPRKQ 308 R M R ++++ + + PR++ Sbjct: 239 RLMPRNITVQILERITRPRRK 259 >Q8XWZ7.1 Q8XWZ7_RALSO Probable short-chain dehydrogenase/reductase sdr; oxidoreductase protein EC=1.-.-.- Length = 275 Score = 258 bits (660), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 128/258 (49%), Positives = 173/258 (67%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + +GK +LITGASSGIG +FA LAK G+ L+LVARS+ KL L AEL R Y + Sbjct: 6 DFRGKTALITGASSGIGREFAYALAKRGAKLLLVARSRDKLHDLTAELRRDYACDADFLT 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + +D+L+NNAGFAT+G FE I RQ +EV++N A +++ Sbjct: 66 VDLSAPDAIPTVAHLLKATGTVVDVLINNAGFATYGRFETIPWTRQRDEVLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R GAVINV+STA FQP PYMA+YGATKAF+LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSDGAVINVASTAAFQPDPYMAIYGATKAFLLSFSEAVWAENRHRGIRVL 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VV+ L A+ + + Y + G +N L A + R Sbjct: 186 ALCPGATQTGFFDVVGAKEAAVGTPMPVASVVQDALWALDRNRSYRIVGTQNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPR 306 ++R S RL + +L PR Sbjct: 246 LLSREMSARLVEKILRPR 263 >B1FBK5.1 B1FBK5_9BURK Short-chain dehydrogenase/reductase SDR Length = 275 Score = 257 bits (657), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 130/259 (50%), Positives = 171/259 (66%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + GK +L+TGASSGIG FA LAK G+ L+LVARS+ KL LAAEL R Y + Sbjct: 6 DFHGKTALVTGASSGIGRAFAYALAKRGARLLLVARSRDKLRDLAAELRRDYACDADFLT 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + ID+LVNNAGFA +G FE I RQ +EV++N A +++ Sbjct: 66 ADLSTTDAVHTIDCHLKVTGKVIDVLVNNAGFAAYGRFETIPWTRQRDEVLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R AG VINV+STA FQP PYMAVYGATKAF+LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSAGVVINVASTAAFQPDPYMAVYGATKAFLLSFSEAVWAENRHRGIRVV 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VV+ L A+ + + + + G RN L A + R Sbjct: 186 ALCPGATQTAFFDVVGAQEAAVGTPMPVASVVQDALWALDRNRSHRIVGTRNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPRK 307 ++R S RL + +L PR Sbjct: 246 LLSREMSARLVEKILRPRD 264 >Q0B2G1.1 Q0B2G1_BURCM Short-chain dehydrogenase/reductase SDR Length = 275 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 129/259 (49%), Positives = 171/259 (66%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + GK +L+TGASSGIG FA LAK G+ L+LVARS+ KL LAAEL R Y + Sbjct: 6 DFHGKTALVTGASSGIGRAFAYALAKRGARLLLVARSRDKLRDLAAELRRDYACDADFLT 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + ID+LVNNAGFA +G FE I RQ +EV++N A +++ Sbjct: 66 ADLSTTDAVHTIDCHLKVTGKVIDVLVNNAGFAAYGRFETIPWARQRDEVLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R AG VINV+STA FQP PYMAVYGATKAF+LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSAGVVINVASTAAFQPDPYMAVYGATKAFLLSFSEAVWAENRHRGIRVV 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VV+ L A+ + + + + G RN L A + R Sbjct: 186 ALCPGATQTAFFDVVGAQEAAVGTPMPVASVVQDALWALDRNRSHRIVGTRNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPRK 307 ++R S R+ + +L PR Sbjct: 246 LLSREMSARMVEKILRPRD 264 >B5SDA3.1 B5SDA3_RALSO Short-chain dehydrogenase/reductase sdr; protein EC=1.-.-.- Length = 305 Score = 247 bits (631), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 123/258 (47%), Positives = 170/258 (65%) Query: 49 NIKGKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVP 108 + +GK +LITGASSGIG +FA LA+ G+ L+LVARS+ KL L AEL R Y + Sbjct: 6 DFRGKTALITGASSGIGREFAYALARRGAKLLLVARSRDKLHDLTAELRRDYACDADFLA 65 Query: 109 QDLSIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDM 168 DLS A + + +D+L+NNAGFA +G FE I Q +E+++N A +++ Sbjct: 66 VDLSTTDAVPTVGHLLKATGTVVDVLINNAGFAAYGRFETIPWTLQRDEMLVNCMAAIEL 125 Query: 169 THLFLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFF 228 THL LPGM R GAVINV+STA FQP PYMA+YGATKA +LSF++A+W ENR RG++ Sbjct: 126 THLLLPGMQARSDGAVINVASTAAFQPDPYMAIYGATKACLLSFSEAVWAENRHRGIRVL 185 Query: 229 ALCPGSTETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISR 288 ALCPG+T+T FF+VVG ++A+VG VV+ L A+ + + Y + G +N L A + R Sbjct: 186 ALCPGATQTGFFDVVGAKEAAVGTPMPVASVVQDALWALDRSRSYRIVGTQNRLLANLQR 245 Query: 289 FMTRRQSLRLAQGMLSPR 306 ++R S RL + +L PR Sbjct: 246 LLSREMSARLVEKILRPR 263 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,677,462,052 Number of extensions: 66948628 Number of successful extensions: 239898 Number of sequences better than 1.0e-05: 23295 Number of HSP's gapped: 219946 Number of HSP's successfully gapped: 23306 Length of query: 308 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 173 Effective length of database: 1,788,859,178 Effective search space: 309472637794 Effective search space used: 309472637794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)