BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0128.p (575 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P08838.3 PT1_BACSU Phosphoenolpyruvate-protein phosphotransferas... 894 0.0 A7Z405.1 A7Z405_BACA2 PtsI EC=2.7.3.9 893 0.0 Q65KB5.1 Q65KB5_BACLD PtsI 885 0.0 A8FCJ7.1 A8FCJ7_BACP2 PTS family phosphoenolpyruvate (PEP) porte... 878 0.0 P42014.1 PT1_BACST Phosphoenolpyruvate-protein phosphotransferas... 875 0.0 >P08838.3 PT1_BACSU Phosphoenolpyruvate-protein phosphotransferase EC=2.7.3.9 Length = 570 Score = 894 bits (2310), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/551 (77%), Positives = 487/551 (88%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + KKN+ D EAE R ++AI+RSK ELEKI E+ ELG DKA+I +A LL Sbjct: 19 YRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELGQDKADIFSAHLL 78 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNPV EKIST+ VNAEFALKE++ FV+M ESMDNEYM+ERA DIRDVT RVT Sbjct: 79 VLSDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVT 138 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV+I +PS+ISEEVII+A DLTPSDTAQLNR +VKGFTTDIGGRTSHSAIMARS+E Sbjct: 139 GHLLGVEIPNPSMISEEVIIVAEDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLE 198 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK A I+NG+ VIVDG+ G+VIIDPS+E + YE K Y AQKA WAK+V+ Sbjct: 199 IPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYEEKHNAYLAQKAEWAKLVN 258 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 EPTV+KDG HVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTE+EQF+AYK Sbjct: 259 EPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYK 318 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCLEEQ+IFRTQLRALL Sbjct: 319 TVLERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALL 378 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIATV+EF+EAKAIL+EEKEKL+ +G +D+IE+GMMVEIPSTAV+ Sbjct: 379 RASTYGNLKIMFPMIATVNEFKEAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVI 438 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 439 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLITLVIEAAHKEGKW 498 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSILPAR QI +LS + A S KE +LSM T+ Sbjct: 499 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTT 558 Query: 560 EEVIEYVKRTF 570 EEV+ +VK TF Sbjct: 559 EEVVAFVKETF 569 >A7Z405.1 A7Z405_BACA2 PtsI EC=2.7.3.9 Length = 571 Score = 893 bits (2307), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/552 (77%), Positives = 487/552 (88%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + KKN+ D AE R ++AI RSK ELEKI E+ ELG DKA+I +A LL Sbjct: 19 YRLEEPDLTVEKKNIADAAAEVSRFDEAIVRSKEELEKIKEHALKELGQDKADIFSAHLL 78 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNPV EKIST+ VNAEFALKE+A FV+M ESMDNEYM+ERA DIRDVT RVT Sbjct: 79 VLSDPELLNPVKEKISTDSVNAEFALKETASMFVTMFESMDNEYMKERAADIRDVTKRVT 138 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV+I +PS+ISEEV+I+A DLTPSDTAQLNR++VKGFTTDIGGRTSHSAIMARS+E Sbjct: 139 GHLLGVEIPNPSMISEEVVIVAEDLTPSDTAQLNRQFVKGFTTDIGGRTSHSAIMARSLE 198 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK A IENG+ VIVDG++G+VIIDPSSE + YE K Y AQKA WAK+V+ Sbjct: 199 IPAVVGTKAATGTIENGVTVIVDGIDGDVIIDPSSETLKKYEEKHSAYLAQKAEWAKLVN 258 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 EPTV+KDG HVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTE+EQF+AYK Sbjct: 259 EPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYK 318 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCLEEQ+IFRTQLRALL Sbjct: 319 TVLERMKGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALL 378 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIATV+EF++AKAIL+EEKEKL+ +GV +D+IE+GMMVEIPSTAV+ Sbjct: 379 RASTYGNLKIMFPMIATVNEFKQAKAILLEEKEKLVEAGVAVSDDIEVGMMVEIPSTAVI 438 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFA EVDF SIGTNDLIQYTMAADRMNERV+YLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 439 ADQFAAEVDFFSIGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLITLVIEAAHKEGKW 498 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSILPAR QI +LS + A S KE +LSM T+ Sbjct: 499 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKKEAESFKETILSMSTT 558 Query: 560 EEVIEYVKRTFD 571 EEV+ +VK TF+ Sbjct: 559 EEVVAFVKETFN 570 >Q65KB5.1 Q65KB5_BACLD PtsI Length = 572 Score = 885 bits (2286), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/553 (76%), Positives = 483/553 (87%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + KKN+ D +AE R E AI RSK ELE I + ELG DKAEI +A LL Sbjct: 19 YRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNHALKELGEDKAEIFSAHLL 78 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNP+ +KI ++ VNAEFALKE+AD FV+M ESMDNEYM+ERA DIRDVT RVT Sbjct: 79 VLSDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDNEYMKERAADIRDVTKRVT 138 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV+I +PSLISEEVII+A DLTPSDTAQLNR +VKGFTTDIGGRTSHSAIMARS+E Sbjct: 139 GHLLGVEIPNPSLISEEVIIVAEDLTPSDTAQLNRNFVKGFTTDIGGRTSHSAIMARSLE 198 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK A I+NG+ VIVDG+ G+VI+DPS E I+ YE K + AQKA WAK+V+ Sbjct: 199 IPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYEEKHQAFLAQKAEWAKLVN 258 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 EPTVT DG HVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTEEEQF+AYK Sbjct: 259 EPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEEEQFDAYK 318 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCL+EQ+IFRTQLRALL Sbjct: 319 TVLERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAIRLCLDEQEIFRTQLRALL 378 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIATV+EF+ AKAIL+EEKEKL A G + +D+IE+GMMVEIPSTAV+ Sbjct: 379 RASTYGNLKIMFPMIATVNEFKAAKAILLEEKEKLKAEGQQVSDDIEVGMMVEIPSTAVI 438 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 439 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLITLVIEAAHKEGKW 498 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIP+L+ LGLDEFSMSATSILPAR QI +LS + S KE +LSM T+ Sbjct: 499 VGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKKETESFKEQILSMSTT 558 Query: 560 EEVIEYVKRTFDI 572 EEV+++VK TF++ Sbjct: 559 EEVVQFVKETFNL 571 >A8FCJ7.1 A8FCJ7_BACP2 PTS family phosphoenolpyruvate (PEP) porter component enzyme I EC=2.7.3.9 Length = 572 Score = 878 bits (2269), Expect = 0.0, Method: Compositional matrix adjust. Identities = 420/551 (76%), Positives = 483/551 (87%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLEEP+L + +K++ D E E +R E+AI SK ELE I E+ ELG DKA+I +A LL Sbjct: 19 YRLEEPDLTVHQKDINDPETEVKRFEEAIHVSKQELEAIKEHALKELGQDKADIFSAHLL 78 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNPV +KI+++ VNAEFALKE+A FV+M E+MDNEYM+ERA DIRDVT RVT Sbjct: 79 VLSDPELLNPVKDKINSDKVNAEFALKETATMFVTMFEAMDNEYMKERAADIRDVTKRVT 138 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 HLLGV I +PS+ISEEVIIIA DLTPSDTAQLNR++VKGF TDIGGRTSHSAIMARSME Sbjct: 139 GHLLGVDIPNPSMISEEVIIIAEDLTPSDTAQLNRQFVKGFATDIGGRTSHSAIMARSME 198 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK+A ++N ++IVDG+ G+VII PS + + Y+ K Y AQKA WAK+V Sbjct: 199 IPAVVGTKEATKTVKNNDLIIVDGISGDVIIQPSEDEVKAYQKKHEDYLAQKAEWAKLVD 258 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 +PTVTKDGVHVELAANIGTP DVKGVL NGGEAVGLYRTEFLYMG+D+LPTEEEQF+AYK Sbjct: 259 QPTVTKDGVHVELAANIGTPEDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEEEQFDAYK 318 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLEKM+GK VVVRTLDIGGDKELPYL LPKEMNPFLG+RAIRLCLEEQ+IFRTQLRALL Sbjct: 319 TVLEKMEGKAVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALL 378 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA +YGNLKIMFPMIAT+SEFREAKAIL+EEKE L+A+G +D+IEIGMMVEIPSTAV+ Sbjct: 379 RASTYGNLKIMFPMIATLSEFREAKAILLEEKEALVAAGTDVSDSIEIGMMVEIPSTAVI 438 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRL+++VI+AAHKEGKW Sbjct: 439 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLITLVIEAAHKEGKW 498 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGD IAIPLL+ LGLDEFSMSATSILPAR Q+ +LS E A S KE +LS+ T+ Sbjct: 499 VGMCGEMAGDEIAIPLLLGLGLDEFSMSATSILPARTQLSKLSKEEAASFKEHILSLSTT 558 Query: 560 EEVIEYVKRTF 570 EEV+++VK+TF Sbjct: 559 EEVVDFVKKTF 569 >P42014.1 PT1_BACST Phosphoenolpyruvate-protein phosphotransferase EC=2.7.3.9 Length = 578 Score = 875 bits (2261), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/553 (75%), Positives = 479/553 (86%) Query: 20 YRLEEPELHILKKNVKDKEAEKQRLEQAISRSKAELEKITEYVNCELGADKAEILAAQLL 79 YRLE P+L K+ V D EAE RLE A++++K ELE I ++ +LG DKA I AA LL Sbjct: 25 YRLETPDLAAEKRTVADVEAEIARLEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLL 84 Query: 80 VLNDPELLNPVFEKISTEGVNAEFALKESADRFVSMLESMDNEYMRERAKDIRDVTNRVT 139 VL+DPELLNP+ EKI TE VNAE+AL E+A F+SM E+MDNEYM+ERA DIRDVT RV Sbjct: 85 VLDDPELLNPIKEKIQTERVNAEYALDETALFFISMFEAMDNEYMKERAADIRDVTKRVL 144 Query: 140 AHLLGVQISDPSLISEEVIIIAGDLTPSDTAQLNRRYVKGFTTDIGGRTSHSAIMARSME 199 AHLLGV IS+PSLISEEV+IIA DLTPSDTAQLNR+YVKGF TDIGGRTSHSAIMARS+E Sbjct: 145 AHLLGVTISNPSLISEEVVIIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLE 204 Query: 200 IPAVVGTKQAVSMIENGIMVIVDGLEGEVIIDPSSEVIALYEAKKVKYEAQKALWAKVVH 259 IPAVVGTK + ++NG +VIVDGL+G+VII+PS E++A YE K+ +YEAQKA WAK+VH Sbjct: 205 IPAVVGTKTVTAEVKNGDIVIVDGLDGQVIINPSPELLAQYEQKRARYEAQKAEWAKLVH 264 Query: 260 EPTVTKDGVHVELAANIGTPADVKGVLANGGEAVGLYRTEFLYMGKDRLPTEEEQFNAYK 319 E TVT DG+HVELAANIGTP DVKG LANG E +GLYRTEFLYMG+ LPTE+EQF AYK Sbjct: 265 EATVTADGIHVELAANIGTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFVAYK 324 Query: 320 TVLEKMDGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEEQDIFRTQLRALL 379 TVLE+M+GKPVVVRTLDIGGDKELPYL LPKEMNPFLGFRAIRLCLE QD+FRTQLRALL Sbjct: 325 TVLEQMNGKPVVVRTLDIGGDKELPYLQLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALL 384 Query: 380 RAGSYGNLKIMFPMIATVSEFREAKAILMEEKEKLIASGVKAADNIEIGMMVEIPSTAVM 439 RA YGNLKIMFPMIAT+ EFR+AKAIL+EEKE L+ GV AD IE+GMMVEIP+ AVM Sbjct: 385 RASVYGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVM 444 Query: 440 ADQFAKEVDFLSIGTNDLIQYTMAADRMNERVSYLYQPYNPAILRLVSMVIDAAHKEGKW 499 ADQFAKEVDF SIGTNDLIQYTMAADRMNERV+YLYQPYNPAILRL+S VIDAAH+EGKW Sbjct: 445 ADQFAKEVDFFSIGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREGKW 504 Query: 500 VGMCGEMAGDPIAIPLLVSLGLDEFSMSATSILPARAQIKELSSEHATSVKEAVLSMKTS 559 VGMCGEMAGDPIAIP+L++LGLDEFSMSATSILPARAQ+K+L+ E A +KE VLS+ T+ Sbjct: 505 VGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKKLAKEEAARIKETVLSLGTA 564 Query: 560 EEVIEYVKRTFDI 572 EEV+ +VKRTF + Sbjct: 565 EEVVSFVKRTFSL 577 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,032,318,167 Number of extensions: 123858698 Number of successful extensions: 426912 Number of sequences better than 1.0e-05: 3221 Number of HSP's gapped: 418786 Number of HSP's successfully gapped: 3370 Length of query: 575 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 434 Effective length of database: 1,732,333,088 Effective search space: 751832560192 Effective search space used: 751832560192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)