BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0123.p (409 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9YDW5.1 B9YDW5_9FIRM Putative uncharacterized protein 393 e-107 Q03GK5.1 Q03GK5_PEDPA Phosphotransferase system cellobiose-speci... 370 e-100 C0CYQ5.1 C0CYQ5_9CLOT Putative uncharacterized protein 332 6e-89 C2BXN7.1 C2BXN7_LISGR PTS system, IIC component 318 4e-85 C0X121.1 C0X121_ENTFA PTS family porter EIIC 307 1e-81 >B9YDW5.1 B9YDW5_9FIRM Putative uncharacterized protein Length = 420 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 193/413 (46%), Positives = 269/413 (65%), Gaps = 9/413 (2%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIPGDVYHIKNY 60 MNKL+ + P MNK+ NVW+ LKDS+MQ LP LLGS+ CLLT+PGD+ + Sbjct: 1 MNKLLTLFEQKLVPTMNKLTRNVWINVLKDSLMQTLPMTLLGSILCLLTVPGDLLGWEWC 60 Query: 61 PDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIVTPQVVKDGT 120 +FW P+ WTMG+LS+ ++ LIP N +EKKKLR+QR A TS+ F IIV P V++DG Sbjct: 61 WNFWTPWNWTMGLLSVFIAVLIPLNYLEKKKLRRQRVAAMMTSIACFFIIVNPIVLEDGV 120 Query: 121 VGFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFDSMLPIGVTI 180 +G ++LG GGM A+++ ++ ++ FGKFSFFK++S +PDFVRAWFD +LP + + Sbjct: 121 LGLAQTALGAGGMLAAMLSGVLASIVLEKFGKFSFFKQDSPMPDFVRAWFDQLLPCAICV 180 Query: 181 ILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGISSWILTPVTK 240 I W+LVD+ +++++++ F P+A V +T +G +++LFI F+YSMGIS W+++P+T Sbjct: 181 IGMWLLVDMAGVNVFHLLQAAFSPIAKVAQTWYGMVILLFIEAFVYSMGISCWVMSPITN 240 Query: 241 PIFLAAIMANATAGAH--------NLVTAETIYSAYLWVGGVACTLPLAFMHLLSKSTKL 292 PI AI+AN + NL T TIY AY GG+ TL L M L SKS K+ Sbjct: 241 PILTMAIVANIEMARNGVTDVALLNLYTQATIYCAYYAFGGIGNTLALNLMSLRSKSNKI 300 Query: 293 KALGRASLVPSIFNINEPLVFGSIVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKV 352 K L RAS+ PSIFNINEP+VFG I WNP +M+PMW+NG++ PL++W+ TK+I F PIP Sbjct: 301 KGLSRASIAPSIFNINEPIVFGMIAWNPFLMVPMWLNGIITPLLMWLFTKIIPFAPIPVT 360 Query: 353 VFD-MWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYEKQELEKE 404 F W +PFP + LI+YPF+K YEKQE+E E Sbjct: 361 DFSGFWCMPFPFVTWLGTQSLNAVILMLGLFVVSWLIYYPFYKVYEKQEIENE 413 >Q03GK5.1 Q03GK5_PEDPA Phosphotransferase system cellobiose-specific component IIC Length = 408 Score = 370 bits (951), Expect = e-100, Method: Compositional matrix adjust. Identities = 195/411 (47%), Positives = 267/411 (64%), Gaps = 11/411 (2%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIPGDVYHIKNY 60 MNK ++ L N F+PK KV NN W++T+K+S++Q+LPFI +GS+ LL IP + + + Sbjct: 1 MNKFMDALNNGFAPKAQKVANNEWILTIKNSILQVLPFIFVGSLITLLGIPTNFWDW--W 58 Query: 61 PDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIVTPQVVKDGT 120 PD +T G+ S+ V+FL P+NLME+KKL KQ+ IAG +S+ FL++ P K + Sbjct: 59 PDLSPISSFTFGLTSIFVAFLFPYNLMERKKLNKQKIIAGLSSVALFLMLANPTWDKTNS 118 Query: 121 V-GFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFDSMLPIGVT 179 + ++ S LG GGMFVA++ IM FGKFSFF E++ +PDFV AWFDSMLPI Sbjct: 119 IISYQFSELGAGGMFVALVVGAFVAIIMGAFGKFSFFGEDTSLPDFVIAWFDSMLPIMTV 178 Query: 180 IILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGISSWILTPVT 239 I + W+L L+H DIY ++++ F PL++ ++T WGF L LF CF+YSMGIS W+L + Sbjct: 179 IGVGWILTYLIHFDIYQLVVSAFSPLSNSLDTWWGFTLFLFFDCFIYSMGISGWVLAAID 238 Query: 240 KPIFLAAIMANATAGAH-----NLVTAETIYSAYLWVGGVACTLPLAFMHLL-SKSTKLK 293 +PI A I NA AH ++ T+E IYS + W+GGV CT+PL M LL KS +LK Sbjct: 239 QPIMFAGIANNAQQFAHGQAVTHVFTSEVIYS-FPWIGGVGCTMPLILMMLLWGKSKRLK 297 Query: 294 ALGRASLVPSIFNINEPLVFGSIVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKVV 353 ALG+A +VPSIFNINEPLVFG+IVWNP +MLP+WING+VLPLI + + PIP + Sbjct: 298 ALGKACIVPSIFNINEPLVFGTIVWNPYLMLPLWINGIVLPLIT-VSWFKLGLSPIPHSL 356 Query: 354 FDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYEKQELEKE 404 MWY+PFP LI+YPFFK Y+K +++E Sbjct: 357 MQMWYIPFPFVTWIVSPAFSAILLLLILFVVGGLIYYPFFKVYDKNLVKEE 407 >C0CYQ5.1 C0CYQ5_9CLOT Putative uncharacterized protein Length = 284 Score = 332 bits (850), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 170/280 (60%), Positives = 210/280 (75%), Gaps = 8/280 (2%) Query: 133 MFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFDSMLPIGVTIILAWVLVDLVHI 192 MF+AIIA + +GYI+ LFGKFSFFKE+SVIPDFVRAWFDS+LPIG+ II+ WVLVD++ + Sbjct: 1 MFIAIIAGIFSGYIVGLFGKFSFFKEDSVIPDFVRAWFDSLLPIGIVIIVVWVLVDILGL 60 Query: 193 DIYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGISSWILTPVTKPIFLAAIMAN-- 250 DIYNII IFMP+A++++TP+GF+L F++CFLYSMGISSW+LTPVTKP F+ I AN Sbjct: 61 DIYNIIQGIFMPVAAIIQTPFGFVLSSFLACFLYSMGISSWVLTPVTKPAFMIGITANIA 120 Query: 251 ------ATAGAHNLVTAETIYSAYLWVGGVACTLPLAFMHLLSKSTKLKALGRASLVPSI 304 AT NLVT T+YSAY+W+GG+ CT PL M +SKS KLKALGRA LVP++ Sbjct: 121 MVAAGTATPETLNLVTDPTVYSAYMWIGGIGCTFPLVIMLAISKSAKLKALGRACLVPAV 180 Query: 305 FNINEPLVFGSIVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKVVFDMWYVPFPXX 364 FNINEP+VFG + WNPIMM+PMW+ +VLPL++W+ TKVI F PIP +VFDMWY PFP Sbjct: 181 FNINEPVVFGCVAWNPIMMIPMWLTSIVLPLVIWVFTKVIPFAPIPTIVFDMWYCPFPIS 240 Query: 365 XXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYEKQELEKE 404 T+IWYPFFK YEKQE+E E Sbjct: 241 TWLTTQSIKGILLMLLCAVIGTVIWYPFFKTYEKQEIENE 280 >C2BXN7.1 C2BXN7_LISGR PTS system, IIC component Length = 422 Score = 318 bits (816), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 168/420 (40%), Positives = 267/420 (63%), Gaps = 15/420 (3%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIPGDVYHIKNY 60 MN++++++ N F+PK+NK+ N W+ ++DS+M LP + +GS+ ++++ +++H Sbjct: 1 MNRIMDFMTNTFAPKVNKIVKNPWISAIQDSIMAALPLVFVGSLVTVISLLRNLFHWM-- 58 Query: 61 PDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIVTPQVVKDGT 120 PDF + ++ GM L+V+F+IP+ LMEKK Q+ I+G T+++ FL+++ P V + G Sbjct: 59 PDFSMISNFSFGMFGLIVAFMIPYYLMEKKGHSGQKLISGSTAVVLFLMLLFPTVQESGE 118 Query: 121 VGFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFDSMLPIGVTI 180 + F S G GMF+AII L G++M+L K S F E++ IPDFV WF+S+LPI + Sbjct: 119 ITFILSRFGATGMFLAIITGLFVGFVMNLAAKRSLFDEDTPIPDFVVGWFNSLLPITFIL 178 Query: 181 ILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGISSWILTPVTK 240 + W++ HID +N++L +F PLAS++++ GF+L +FI FLY+ GIS W++ PV Sbjct: 179 VAGWLVTVQFHIDFFNVVLLVFSPLASIIQSYPGFVLSVFIPVFLYTFGISGWVMMPVIY 238 Query: 241 PIFLAAIMANATAGAH-----NLVTAETIYSAYLWVGGVACTLPLAFMHLL-SKSTKLKA 294 P+++A + ANA A AH N+ T ETIY A+ +GG+ TL L+FM LL S+S +LKA Sbjct: 239 PVYMAGLAANADAVAHGAKAVNIATQETIY-AFSSMGGIGTTLALSFMMLLISRSAQLKA 297 Query: 295 LGRASLVPSIFNINEPLVFGS-IVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKVV 353 +G+A +PSIFNINEPLVFG+ I +NP +M+PMWIN L++P + ++ + IP Sbjct: 298 IGKAVFIPSIFNINEPLVFGAPIAFNPYLMIPMWINSLLVPSLAYLSMS-LGLVNIPTHS 356 Query: 354 FDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYE----KQELEKELPQAN 409 F +WY+P+P +I+ PFFKAY+ KQE ++ L ++ Sbjct: 357 FLLWYMPYPFTSYLATQDFRAVIVCIVIFAITWVIFLPFFKAYDNSLLKQEAKENLEESQ 416 >C0X121.1 C0X121_ENTFA PTS family porter EIIC Length = 416 Score = 307 bits (787), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 168/411 (40%), Positives = 259/411 (63%), Gaps = 11/411 (2%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIPGDVYHIKNY 60 M K+++++ N F+PK+NKV N W+ ++D++M LP + +GS+ ++++ +++ + Sbjct: 1 MKKIMDFMTNSFAPKVNKVVKNPWISAIQDAIMAALPLVFVGSLVTVVSLLKNLF--PSM 58 Query: 61 PDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIVTPQVVKDGT 120 PDF + ++ GM L+VSF+IP+ LMEKK Q+ IAG TS++ F++++ P + + G Sbjct: 59 PDFSMISNFSFGMFGLIVSFMIPYYLMEKKGHSGQKLIAGATSIVLFMMLLFPTLSEKGE 118 Query: 121 VGFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFDSMLPIGVTI 180 + F S G GMF+A+I L +M L K SFF E++ IPDFV WF+S+LPI + Sbjct: 119 ITFILSRFGATGMFLAMITGLFVSLVMHLAAKKSFFDEDTPIPDFVVGWFNSLLPITFIL 178 Query: 181 ILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGISSWILTPVTK 240 + W++ ID ++I++ IF PLA++V++ GF+L +FI FLY+ GIS W++ PV Sbjct: 179 FVGWLITIQFRIDFFDIVILIFSPLANIVQSYPGFVLSVFIPVFLYTFGISGWVMMPVIY 238 Query: 241 PIFLAAIMANATA---GAH--NLVTAETIYSAYLWVGGVACTLPLAFMH-LLSKSTKLKA 294 P+++A + ANA A G H N+ T ET Y A+ +GG+ TL L+ M LLSKS +LKA Sbjct: 239 PVYMAGLAANAEAVANGGHAVNIATQETCY-AFSSMGGIGATLSLSVMMVLLSKSAQLKA 297 Query: 295 LGRASLVPSIFNINEPLVFGS-IVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKVV 353 +G+A L+PSIFNINEPLVFG+ I +NP +M+PMWIN LV+P I + T F IP Sbjct: 298 IGKAVLIPSIFNINEPLVFGAPIAFNPYLMIPMWINALVVPSIAY-ATMYWGFVNIPTQS 356 Query: 354 FDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYEKQELEKE 404 F +WY+P+P L++ PFFKAY++ L++E Sbjct: 357 FLLWYMPYPFTSYLATQDIRAVVVCVLIFVITWLVFLPFFKAYDQSLLKQE 407 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.143 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,416,606,459 Number of extensions: 97209468 Number of successful extensions: 354383 Number of sequences better than 1.0e-05: 1492 Number of HSP's gapped: 350525 Number of HSP's successfully gapped: 1504 Length of query: 409 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 271 Effective length of database: 1,760,596,133 Effective search space: 477121552043 Effective search space used: 477121552043 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)