BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0119.p (236 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D5J1.1 B1D5J1_9BACL Short-chain dehydrogenase/reductase SDR 343 7e-93 Q8VMZ9.1 Q8VMZ9_LISGR Putative uncharacterized protein 291 4e-77 C2C4C6.1 C2C4C6_LISGR Possible dehydrogenase 279 2e-73 Q4MUG7.1 Q4MUG7_BACCE Short chain dehydrogenase 189 2e-46 C2QRC1.1 C2QRC1_BACCE Short chain dehydrogenase 188 4e-46 >B1D5J1.1 B1D5J1_9BACL Short-chain dehydrogenase/reductase SDR Length = 236 Score = 343 bits (880), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 167/236 (70%), Positives = 191/236 (80%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 M K A VTGANKGIGFEIA+QLGE GW+V+LGAR +RG AV+EL++QGLD E++QIDM Sbjct: 1 MKKTAFVTGANKGIGFEIAKQLGEKGWRVVLGARDAQRGHVAVSELSAQGLDAEWIQIDM 60 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 T L SIE A+ ++ YP L LLINNAGMPGAFSSSF++T+EE LR AFEVNFFGT+RLN Sbjct: 61 TDLTSIETASQIIKTKYPALKLLINNAGMPGAFSSSFTDTKEEDLRNAFEVNFFGTYRLN 120 Query: 121 QRLLPLITENDGTIVNVSTDMASLHFMQNAEYALNAFDYNSSKTANNAMTVAMAIELKNS 180 QRL LI +N+GTIVNVSTDMASL+ MQNAE+ LNAFDYNSSKTANNAMT++MA EL+NS Sbjct: 121 QRLFSLIKDNEGTIVNVSTDMASLNHMQNAEFTLNAFDYNSSKTANNAMTLSMAYELRNS 180 Query: 181 KAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 AQVFAVTPGFT TDLN IIVGYATDGKRHNGEFL+ GV AW Sbjct: 181 VAQVFAVTPGFTTTDLNGNAEGGKSKAEGAAIIVGYATDGKRHNGEFLNEQGVIAW 236 >Q8VMZ9.1 Q8VMZ9_LISGR Putative uncharacterized protein Length = 239 Score = 291 bits (744), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 149/239 (62%), Positives = 169/239 (70%), Gaps = 3/239 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 MTK ALVTGANKGIG E R+L GWKV+LGAR E+GK AV EL +QGLDV FL+ID+ Sbjct: 1 MTKSALVTGANKGIGLETVRRLAGKGWKVVLGARDAEKGKQAVTELQAQGLDVAFLEIDL 60 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 ES+E A + YP ++LLINNAGMPG F+ FS T EE LR AFEVNFFGTFRLN Sbjct: 61 VDKESVESAVKIITQEYPDISLLINNAGMPGEFAKGFSKTTEEGLRNAFEVNFFGTFRLN 120 Query: 121 QRLLPLITENDGTIVNVSTDMASLHFM---QNAEYALNAFDYNSSKTANNAMTVAMAIEL 177 Q LLP + N TIVNVS DMASL M Q ALN+FDYN+SKTANNAMTV+MA+EL Sbjct: 121 QLLLPSLKGNGATIVNVSIDMASLSLMMDHQERTAALNSFDYNASKTANNAMTVSMALEL 180 Query: 178 KNSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 K+S AQVFAVTPGFT TDLN II+ + DG+RHNG F D NGV AW Sbjct: 181 KDSPAQVFAVTPGFTTTDLNNNAPGGITKEEAAGIILKHTLDGERHNGVFFDKNGVLAW 239 >C2C4C6.1 C2C4C6_LISGR Possible dehydrogenase Length = 251 Score = 279 bits (713), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 152/239 (63%), Positives = 170/239 (71%), Gaps = 3/239 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 MTK ALVTGANKGIG E RQL GWKV+LGAR E+GK AV EL +QGLDV FL+ID+ Sbjct: 13 MTKSALVTGANKGIGLETVRQLAGKGWKVVLGARDAEKGKQAVTELQAQGLDVAFLEIDL 72 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 ES+E A + YP ++LLINNAGMPG F+ FS T EE LR AFEVNFFGTFRLN Sbjct: 73 VDKESVESAVKIITQEYPDISLLINNAGMPGEFAKGFSKTTEEGLRNAFEVNFFGTFRLN 132 Query: 121 QRLLPLITENDGTIVNVSTDMASLHFM---QNAEYALNAFDYNSSKTANNAMTVAMAIEL 177 Q LLPL+ N TIVNVS DMASL M Q ALN+FDYN+SKTANNAMTV+MA+EL Sbjct: 133 QLLLPLLKGNGATIVNVSIDMASLSLMMDHQERTAALNSFDYNASKTANNAMTVSMALEL 192 Query: 178 KNSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 K+S AQVFAVTPGFT TDLN II+ Y DG+RHNG F D NGV AW Sbjct: 193 KDSPAQVFAVTPGFTTTDLNNNAPGGITKEEAAGIILKYTLDGERHNGVFFDKNGVLAW 251 >Q4MUG7.1 Q4MUG7_BACCE Short chain dehydrogenase Length = 236 Score = 189 bits (479), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 104/238 (43%), Positives = 146/238 (61%), Gaps = 4/238 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 M K A +TGANKGIG+E+ RQL E + V LGAR+ + G+ AV L +V ++Q+D+ Sbjct: 1 MKKYAFITGANKGIGYELVRQLAEKDYHVFLGARNKQLGQQAVESLHVS--NVSYIQVDI 58 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 +S +SI++A + L LLINNAG+ F++ S E LR+ FEVNFFGTF++ Sbjct: 59 SSSQSIQEAMKKIHETTDHLDLLINNAGIALDFNTLPSELNIETLRQGFEVNFFGTFQMM 118 Query: 121 QRLLPLITENDGT-IVNVSTDMASLHFMQNAE-YALNAFDYNSSKTANNAMTVAMAIELK 178 Q LPL+ ++ + I+NV+TDMASL N E + +NA YNSSKTA NA+T+A + E Sbjct: 119 QAFLPLLKKSSKSKIINVTTDMASLTMFANGETHPINALGYNSSKTAINALTLAFSKEFS 178 Query: 179 NSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 S ++F VTPGFT TDLN II+ YA +NG+ L+ +G+ W Sbjct: 179 TSGPEIFGVTPGFTTTDLNGNSPGGHTTNESAKIIIKYALSETNYNGKILNKDGIITW 236 >C2QRC1.1 C2QRC1_BACCE Short chain dehydrogenase Length = 236 Score = 188 bits (477), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 103/238 (43%), Positives = 145/238 (60%), Gaps = 4/238 (1%) Query: 1 MTKIALVTGANKGIGFEIARQLGEAGWKVLLGARSIERGKAAVAELTSQGLDVEFLQIDM 60 M K A +TGANKGIG+E+ RQL E + V LGAR+ + G+ AV L +V ++Q+D+ Sbjct: 1 MKKYAFITGANKGIGYELVRQLAEKDYHVFLGARNKQLGQQAVESLHVS--NVSYIQVDI 58 Query: 61 TSLESIEQAAGTLRNNYPGLTLLINNAGMPGAFSSSFSNTQEEHLREAFEVNFFGTFRLN 120 +S +SI++A + L LLINNAG+ F++ S E LR+ FEVNFFGTF++ Sbjct: 59 SSSQSIQEAMKKIHETTDHLDLLINNAGIALDFNTLPSELNIETLRQGFEVNFFGTFQMM 118 Query: 121 QRLLPLITENDGT-IVNVSTDMASLHFMQNAE-YALNAFDYNSSKTANNAMTVAMAIELK 178 Q LPL+ ++ + I+NV+TDMASL N E + +N YNSSKTA NA+T+A + E Sbjct: 119 QAFLPLLKKSSKSKIINVTTDMASLTMFANGETHPINTLGYNSSKTAINALTLAFSKEFS 178 Query: 179 NSKAQVFAVTPGFTKTDLNXXXXXXXXXXXXXXIIVGYATDGKRHNGEFLDINGVYAW 236 S ++F VTPGFT TDLN II+ YA +NG+ L+ +G+ W Sbjct: 179 TSGPEIFGVTPGFTTTDLNGNSPGGHTTNESAKIIIKYALSATNYNGKILNKDGIIPW 236 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,196,968,205 Number of extensions: 42330694 Number of successful extensions: 199371 Number of sequences better than 1.0e-05: 18107 Number of HSP's gapped: 190553 Number of HSP's successfully gapped: 18120 Length of query: 236 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 104 Effective length of database: 1,817,122,223 Effective search space: 188980711192 Effective search space used: 188980711192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)