BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0104.p (469 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZFV9.1 C0ZFV9_BREBN Aspartate ammonia-lyase EC=4.3.1.1 696 0.0 A7GL70.1 A7GL70_BACCN Aspartate ammonia-lyase 694 0.0 C3HVN7.1 C3HVN7_BACTU Aspartate ammonia-lyase 691 0.0 B7IWY7.1 B7IWY7_BACC2 Aspartate ammonia-lyase EC=4.3.1.1 690 0.0 A4IQM0.1 A4IQM0_GEOTN L-aspartase 680 0.0 >C0ZFV9.1 C0ZFV9_BREBN Aspartate ammonia-lyase EC=4.3.1.1 Length = 474 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/462 (73%), Positives = 400/462 (86%) Query: 6 RVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDIHR 65 RVE DFLGE ++P DAYYG+QT+RA ENFPITGY +H LI +MAMVKKAAA+ANM++ R Sbjct: 7 RVEKDFLGEKQIPVDAYYGVQTMRATENFPITGYRLHFSLINAMAMVKKAAAMANMEVSR 66 Query: 66 LPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGVKGD 125 L R+G+ IV AA+EI+ G W +QF VDPIQGGAGTSINMNANEVIANR +E+LG KGD Sbjct: 67 LNPRLGNAIVAAAEEIMQGKWHDQFIVDPIQGGAGTSINMNANEVIANRGLEMLGEKKGD 126 Query: 126 YTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGIIKMG 185 Y +SPN+HVNMSQSTND+FPTA HIA L L+++LLVTM +H AF +KA+EFD +IKMG Sbjct: 127 YFHLSPNTHVNMSQSTNDAFPTAIHIATLTLLEKLLVTMDKMHDAFGQKAKEFDDVIKMG 186 Query: 186 RTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRYISK 245 RTHLQDAVPIRLGQEF AY R+LERD+ RI+Q++ HLYE+NMGATAVGTGLNADPRYI+ Sbjct: 187 RTHLQDAVPIRLGQEFEAYRRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNADPRYITT 246 Query: 246 VVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGPRAG 305 VV+ LADI+G+PL A HL DATQNTD YTEVSAALK+ M+NMSK ANDLRLMASGPRAG Sbjct: 247 VVKHLADITGFPLTGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAG 306 Query: 306 LGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVMEPVL 365 LGEI LPARQPGSSIMPGKVNPVM E++NQVAFQV+GNDHTIC+ASEAGQLELNVMEPVL Sbjct: 307 LGEISLPARQPGSSIMPGKVNPVMAELINQVAFQVIGNDHTICLASEAGQLELNVMEPVL 366 Query: 366 VFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAARIA 425 VFNLLQS+SIM+ A F +HC++GI ++ER + YV++SVGVITA+NP+LGYE AARIA Sbjct: 367 VFNLLQSISIMDNAFDVFTRHCLEGIEANRERMKEYVEKSVGVITAVNPHLGYETAARIA 426 Query: 426 HEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAGS 467 EAI TG+SVR LCL++N+L EEELD IL+P+ MT PGIAG+ Sbjct: 427 REAILTGKSVRELCLQHNVLTEEELDLILDPFEMTHPGIAGA 468 >A7GL70.1 A7GL70_BACCN Aspartate ammonia-lyase Length = 477 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/465 (70%), Positives = 396/465 (85%) Query: 3 KLVRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMD 62 K +R+E DFLGE EVP +AYYG+QTLRA+ENFPITGY IH+ LIT+MA+VKKAAALAN++ Sbjct: 5 KDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAALANVE 64 Query: 63 IHRLPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGV 122 LP IG I AA EI+ G +QF VDPIQGGAGTSINMNANEVIANRA+E +G Sbjct: 65 TGYLPENIGKEIAAAAQEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALERMGYE 124 Query: 123 KGDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGII 182 KGDY ISPN+HVNM+QSTND+FPT HIA L +++ELL+TM LH AF++KA+EFD +I Sbjct: 125 KGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEELLITMEELHRAFIKKAKEFDHVI 184 Query: 183 KMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRY 242 KMGRTHLQDAVPIRLGQEF+AY+R+LERD+ RI+Q++ HLYE+NMGATAVGTGLNA+P Y Sbjct: 185 KMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNANPMY 244 Query: 243 ISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGP 302 I +VV+ L ++SG+PL A HL DATQNTD YTEVSAALK+ M+NMSK ANDLR+MASGP Sbjct: 245 IEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGP 304 Query: 303 RAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVME 362 R GL EI+LPARQPGSSIMPGKVNPVM EV+NQVAFQV+GNDHTIC+ASEAGQLELNVME Sbjct: 305 RVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVME 364 Query: 363 PVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAA 422 PVLVFNL+QS+SIMN A F+++CI+GIT ++E + YV++SVG+ITA+NP++GYE A+ Sbjct: 365 PVLVFNLIQSISIMNNAFRVFREYCIEGITANEELLKEYVERSVGIITAVNPHIGYEAAS 424 Query: 423 RIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAGS 467 RIA EAI TG+SVR LCL + +L EEELD IL+PY MT P IAG+ Sbjct: 425 RIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGA 469 >C3HVN7.1 C3HVN7_BACTU Aspartate ammonia-lyase Length = 477 Score = 691 bits (1783), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/465 (70%), Positives = 392/465 (84%) Query: 3 KLVRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMD 62 K +R+E DFLGE EVP+ AYYG+QTLRA+ENFPITGY IH LIT+MA+VKKAAALAN+D Sbjct: 5 KDIRIEKDFLGEKEVPSAAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAALANID 64 Query: 63 IHRLPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGV 122 L IGH I +AA EI+ G + +QF VDPIQGGAGTSINMN NEVIANRA+E +G Sbjct: 65 TGYLAKDIGHEIAEAAQEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALERMGYE 124 Query: 123 KGDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGII 182 KG+Y ISPN+HVNM+QSTND+FPT HIA L +++ELL+TM LH AF +KA+EFD +I Sbjct: 125 KGEYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEELLITMEELHSAFRKKAKEFDHVI 184 Query: 183 KMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRY 242 KMGRTHLQDAVPIRLGQEF AY+R+LERD+ RI+Q++ HLYE+NMGATAVGTGLNA+P Y Sbjct: 185 KMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNANPTY 244 Query: 243 ISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGP 302 I +VV+ L SG+PL A HL DATQNTD YTEVSAALK+ M+NMSK ANDLR+MASGP Sbjct: 245 IEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGP 304 Query: 303 RAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVME 362 R GL EI+LPARQPGSSIMPGKVNPVM EV+NQVAFQV+GNDHTIC+ASEAGQLELNVME Sbjct: 305 RVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVME 364 Query: 363 PVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAA 422 PVLVFNL+QS+SIMN F+++CI+GIT ++E + YV++SVG+ITA+NP++GYE A+ Sbjct: 365 PVLVFNLIQSISIMNNGFRVFREYCIEGITANEELLKQYVEKSVGIITAVNPHIGYEAAS 424 Query: 423 RIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAGS 467 RIA EAI+TG+SVR LCL + +L EEELD IL+P+ MT P IAGS Sbjct: 425 RIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGS 469 >B7IWY7.1 B7IWY7_BACC2 Aspartate ammonia-lyase EC=4.3.1.1 Length = 477 Score = 690 bits (1780), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/465 (70%), Positives = 392/465 (84%) Query: 3 KLVRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMD 62 K +R+E DFLGE EVP+ AYYG+QTLRA+ENFPITGY IH LIT+MA+VKKAAALAN+D Sbjct: 5 KDIRIEKDFLGEKEVPSAAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAALANID 64 Query: 63 IHRLPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGV 122 L IGH I +AA EI+ G + +QF VDPIQGGAGTSINMN NEVIANRA+E +G Sbjct: 65 TGYLAKDIGHEIAEAAQEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALERMGYE 124 Query: 123 KGDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGII 182 KG+Y ISPN+HVNM+QSTND+FPT HIA L +++ELL+TM LH AF +KA+EFD +I Sbjct: 125 KGEYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEELLITMEELHSAFRKKAKEFDHVI 184 Query: 183 KMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRY 242 KMGRTHLQDAVPIRLGQEF AY+R+LERD+ RI+Q++ HLYE+NMGATAVGTGLNA+P Y Sbjct: 185 KMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNANPTY 244 Query: 243 ISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGP 302 I +VV+ L SG+PL A HL DATQNTD YTEVSAALK+ M+NMSK ANDLR+MASGP Sbjct: 245 IEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGP 304 Query: 303 RAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVME 362 R GL EI+LPARQPGSSIMPGKVNPVM EV+NQVAFQV+GNDHTIC+ASEAGQLELNVME Sbjct: 305 RVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVME 364 Query: 363 PVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAA 422 PVLVFNL+QS+SIMN F+++CI+GIT ++E + YV++SVG+ITA+NP++GYE A+ Sbjct: 365 PVLVFNLIQSISIMNNGFRVFREYCIEGITANEELLKQYVEKSVGIITAVNPHIGYEAAS 424 Query: 423 RIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAGS 467 RIA EAI+TG+SVR LCL + +L EEELD IL+P+ MT P IAG+ Sbjct: 425 RIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGA 469 >A4IQM0.1 A4IQM0_GEOTN L-aspartase Length = 478 Score = 680 bits (1755), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/468 (71%), Positives = 388/468 (82%) Query: 2 NKLVRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANM 61 N VR+E DFLG+ EVP DAYYGIQTLRA+ENFPITGY IH+ LI ++AMVKKAAALANM Sbjct: 7 NSKVRIERDFLGDKEVPIDAYYGIQTLRALENFPITGYRIHKELIKALAMVKKAAALANM 66 Query: 62 DIHRLPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGG 121 D RL + IG IV+AADEIIAG W E F VDPIQGGAGTSINMNANEVIANRA+ELLG Sbjct: 67 DTKRLKAEIGEAIVKAADEIIAGKWHEHFIVDPIQGGAGTSINMNANEVIANRALELLGY 126 Query: 122 VKGDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGI 181 KG+Y +SPN+HVNMSQSTND FPTA HIA+L L+D+LL TM +H KA +FD + Sbjct: 127 EKGEYGYLSPNNHVNMSQSTNDVFPTAVHIALLNLLDKLLHTMRYMHAVMKEKARQFDHV 186 Query: 182 IKMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPR 241 IK+GRTHLQDAVPIRLGQEF AY+ +LERD+ RI +++ +LYEINMGATAVGTGLNADPR Sbjct: 187 IKIGRTHLQDAVPIRLGQEFEAYSCVLERDIQRIDRSRQYLYEINMGATAVGTGLNADPR 246 Query: 242 YISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASG 301 YI VV+ L++ISG PL A HL DATQNTD YTEVS+ALK+ MINMSK ANDLRLMASG Sbjct: 247 YIEGVVKYLSEISGLPLVRAEHLVDATQNTDAYTEVSSALKVCMINMSKIANDLRLMASG 306 Query: 302 PRAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVM 361 PRAGLGEI LP RQPGSSIMPGKVNPVM EV+NQVAFQV+GNDHTIC+ASEAGQLELNVM Sbjct: 307 PRAGLGEITLPPRQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVM 366 Query: 362 EPVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEA 421 EPVLVFNLLQS++IM A F +C+ GI ++ER YV++SV V+TA++PY+GYE A Sbjct: 367 EPVLVFNLLQSITIMTNAFHVFTNYCLKGIEANEERLRHYVEKSVSVVTAVSPYIGYETA 426 Query: 422 ARIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAGSVT 469 ARIA +AI GES+R LCL+Y LL E+ELD IL+PY+MT PGI ++ Sbjct: 427 ARIAQQAILEGESIRKLCLQYGLLTEKELDHILDPYKMTSPGILSVIS 474 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,622,025,246 Number of extensions: 103480599 Number of successful extensions: 265035 Number of sequences better than 1.0e-05: 3627 Number of HSP's gapped: 261029 Number of HSP's successfully gapped: 3630 Length of query: 469 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 330 Effective length of database: 1,751,175,118 Effective search space: 577887788940 Effective search space used: 577887788940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)