BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0103.p (395 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5DA98.1 C5DA98_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 507 e-142 P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 503 e-140 B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 503 e-140 Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 502 e-140 C3J102.1 C3J102_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 501 e-140 >C5DA98.1 C5DA98_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 478 Score = 507 bits (1305), Expect = e-142, Method: Compositional matrix adjust. Identities = 243/387 (62%), Positives = 300/387 (77%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +I ++DR F H GGKI T PI DDL+R+YTPGVA+VC AI EDP++ Y+LT+KR Sbjct: 80 IIHVSDRTFWMHIGGKIETNSKFPIKTRDDLSRVYTPGVARVCKAIAEDPKKAYSLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAV++DGTAVLGLGNIGPYAA PVMEGKA+LFKQ AG++AFPICLDT DTEEII+ VK Sbjct: 140 NTVAVISDGTAVLGLGNIGPYAAMPVMEGKAMLFKQFAGVDAFPICLDTKDTEEIIQIVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+P CF PVFHDDQHGTA+V+LAGL+NALK+ GK+ Sbjct: 200 AIAPAFGGINLEDISAPHCFEIERRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVGKK 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++VV G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 260 LEDIKVVVTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDEKYDNPYWQEYAQITNPD 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 ++ G+L EVIAGADVFIGVS GIL+ DV++MA PIVFAMANP PEI PELA PY V Sbjct: 320 NEKGSLSEVIAGADVFIGVSAPGILKVDDVKKMAKDPIVFAMANPVPEIDPELAEPYVRV 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV D ELN YII Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVTDDELNETYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAVIEAA +G+A+ Sbjct: 440 PSVFNSKVVERVRQAVIEAAYRTGVAR 466 >P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 Length = 478 Score = 503 bits (1296), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/387 (61%), Positives = 303/387 (78%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +++++DR FL H GGKI T +P+ DDL+R+YTPGVA+VCTAI EDP + Y+LT+KR Sbjct: 80 IVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DGTAVLGLG+IGPYAA PVMEGKA+LFK+ AG++AFPICLDT DTEEII+ VK Sbjct: 140 NTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLDTKDTEEIIQIVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+PRCF PVFHDDQHGTA+V+LAGL+NALK+ K+ Sbjct: 200 AIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKK 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++V+ G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 260 LEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQEYAQLTNPD 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L +VIAGADVFIGVS GIL+ +DV++MA PIVFAMANP PEI PELA PY V Sbjct: 320 NLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPYVRV 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV + ELN YII Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAV+EAA +G+A+ Sbjct: 440 PSVFNSKVVERVRQAVVEAAYRTGVAR 466 >B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 470 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/387 (61%), Positives = 302/387 (78%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +++++DR FL H GGKI T +P+ DDL+R+YTPGVA+VCTAI EDP + Y+LT+KR Sbjct: 72 IVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKR 131 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DGTAVLGLG+IGPYAA PVMEGKA+LFKQ AG++AFP+CLDT DTEEII+ VK Sbjct: 132 NTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKQFAGVDAFPVCLDTKDTEEIIQIVK 191 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+PRCF PVFHDDQHGTA+V+LAGL+NALK+ K+ Sbjct: 192 AIAPAFGGINLEDISAPRCFEIEARLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKK 251 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++V+ G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 252 LEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRYGAIHRDETYENPYWQEYAQITNPD 311 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L +VI GADVFIGVS GIL+ +DV++MA PIVFAMANP PEI PELA PY V Sbjct: 312 NLKGSLSDVIVGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPVPEIDPELAEPYVRV 371 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV + ELN YII Sbjct: 372 MATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVTEDELNETYII 431 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAVIEAA +G+A+ Sbjct: 432 PSVFNSKVVERVRQAVIEAAYRTGVAR 458 >Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 Length = 478 Score = 502 bits (1293), Expect = e-140, Method: Compositional matrix adjust. Identities = 239/387 (61%), Positives = 303/387 (78%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +I+++DR FL H GGKI T +P+ DDL+R+YTPGVA+VCTAI EDP + Y+LT+KR Sbjct: 80 IINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DGTAVLGLG+IGPYAA PVMEGKA+LFK+ AG++AFPICL+T DTEEII+ VK Sbjct: 140 NTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTKDTEEIIQIVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+PRCF PVFHDDQHGTA+V+LAGL+NALK+ K+ Sbjct: 200 AIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKK 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++V+ G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 260 LEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQEYAQITNPD 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L +VIAGADVFIGVS GIL+ +DV++MA PIVFAMANP PEI PELA PY V Sbjct: 320 NLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPYVRV 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV + ELN YII Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAVIEAA +G+A+ Sbjct: 440 PSVFNSKVVERVRQAVIEAAYRTGVAR 466 >C3J102.1 C3J102_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 478 Score = 501 bits (1291), Expect = e-140, Method: Compositional matrix adjust. Identities = 239/387 (61%), Positives = 303/387 (78%) Query: 1 MIDITDRIFLAHQGGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKR 60 +I+++DR FL H GGKI T +P+ DDL+R+YTPGVA+VCTAI EDP + Y+LT+KR Sbjct: 80 IINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKR 139 Query: 61 NMVAVVTDGTAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVK 120 N VAVV+DGTAVLGLG+IGPYAA PVMEGKA+LFK+ AG++AFPICL+T DTEEII+ VK Sbjct: 140 NTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTKDTEEIIQIVK 199 Query: 121 AIAPIFGGINLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQ 180 AIAP FGGINLEDIS+PRCF PVFHDDQHGTA+V+LAGL+NALK+ K+ Sbjct: 200 AIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKK 259 Query: 181 IDHVRIVVNGVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVE 240 ++ +++V+ G+GAAG++ ++LLAAG R+++ VD+ GA+ R Y +P WQ A + + Sbjct: 260 LEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQEYAQITNPD 319 Query: 241 DQPGTLQEVIAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPYAGV 300 + G+L +VIAGADVFIGVS GIL+ +DV++MA PIVFAMANP PEI PELA PY V Sbjct: 320 NLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPYVRV 379 Query: 301 YATGRSDYPNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYII 360 ATGRSDYPNQINNVL FPGIF+GAL+CRA+ IN++MKL VV + ELN YII Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYII 439 Query: 361 PSLFNDQVVIKVRQAVIEAAVSSGIAK 387 PS+FN +VV +VRQAVIEAA +G+A+ Sbjct: 440 PSVFNSKVVERVRQAVIEAAYRTGVAR 466 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,272,270,259 Number of extensions: 92981103 Number of successful extensions: 233086 Number of sequences better than 1.0e-05: 2160 Number of HSP's gapped: 228662 Number of HSP's successfully gapped: 2165 Length of query: 395 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 257 Effective length of database: 1,760,596,133 Effective search space: 452473206181 Effective search space used: 452473206181 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)