BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0102.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GK60.1 B7GK60_ANOFW Maltose permease, MFS type 152 8e-35 C5D6C8.1 C5D6C8_9BACI Major facilitator superfamily MFS_1 149 5e-34 P39589.1 YWBF_BACSU Uncharacterized transporter ywbF 146 4e-33 C0ZL72.1 C0ZL72_BREBN Probable maltose permease 145 6e-33 B1YKG6.1 B1YKG6_EXIS2 Major facilitator superfamily MFS_1 136 3e-30 >B7GK60.1 B7GK60_ANOFW Maltose permease, MFS type Length = 383 Score = 152 bits (383), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 99/356 (27%), Positives = 183/356 (51%), Gaps = 16/356 (4%) Query: 11 HVYNYFFYIAYASYLPFISYWFAED-GLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGM 69 ++ + + ++ S P +S + ++ GLS +IG+I SI P+V +QP+WGM+ DY Sbjct: 7 RIFYFLQFFSFGSLFPLLSVYLKDEVGLSGTEIGMIMSISPIVMIFIQPIWGMISDYTRK 66 Query: 70 AKWLLLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHAKHNSL 129 +L++S T + Y F + WL +VI+ +LAV S +P+ D++T+ + + + Sbjct: 67 PVQVLIVSLLGTAFFGFMYSFVHSYAWL-LVIAALLAVTQSGIVPLSDSITLHYVQKANE 125 Query: 130 SYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRKGRPKP 189 YG+IR+ G++ F AV V GQ+ E F + +F ++ + + LL + +P + Sbjct: 126 RYGSIRLWGALGFAAAVPVAGQVAEWFDLRFIFYIFVALLIISSLLAWKLPRE------- 178 Query: 190 EMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGP-QMNNAFYSVYISHFGGEASA 248 + + + + GM L++ P+F+ + GP NN ++ + I+ GG + Sbjct: 179 ----QSSAKVNIREGMKELIRIPRFL-LFLLTTFLVFGPVYANNFYFGILITDLGGTLTG 233 Query: 249 KIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMANQ 308 +GL + A+ +E P F+ ++RKFG + +L + RW EP I+ A Sbjct: 234 -VGLAFLLAAGSEAPFMKFADGVVRKFGMMNVLFVATALSCARWLFYFFEPPLMIVYATT 292 Query: 309 MLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVER 364 ++ G + LF+ A + Y D + +TTA SL+ V + + VGG+++ER Sbjct: 293 IVQGASVGLFIPAALQYVRDITPAHVRTTAVSLYATVGNGLGSWFCTFVGGYILER 348 >C5D6C8.1 C5D6C8_9BACI Major facilitator superfamily MFS_1 Length = 392 Score = 149 bits (376), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 102/341 (29%), Positives = 171/341 (50%), Gaps = 16/341 (4%) Query: 26 PFISYWFAED-GLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGMAKWLLLISTGITPWV 84 P +S + E GLS +IG+I SI P+ VQP+WGM+ DY ++L I+ T ++ Sbjct: 32 PLLSVYLQEVVGLSGTEIGVIMSISPVTMIFVQPIWGMITDYTRKPVFVLTIALLATSFI 91 Query: 85 VIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHAKHNSLSYGAIRVVGSISFGL 144 + Y F + WL IV++V+LA SA +P+ D++T+ +A+ N YG+IR+ GSI F + Sbjct: 92 GLMYSFVHEYHWL-IVMAVLLASAQSAIVPISDSITLHYAQKNGEKYGSIRLWGSIGFAV 150 Query: 145 AVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRKGRPKPEMAGKGAPRSGMTAG 204 AV + G + + + +F + + L LL +P + + ++ G Sbjct: 151 AVLIGGWLSDRIALVVIFYLFSFMLILSGLLASRLPKESQTM-----------KAEALRG 199 Query: 205 MITLLKEPKFVWFLIPVFLAAIGPQMNNAFY-SVYISHFGGEASAKIGLLYTAASLTEIP 263 M L + P FV L+ F GP + N FY ++I GG + IGL + A+ +E P Sbjct: 200 MAQLFRIPHFVLLLLATFF-IFGPILANNFYFGIFIKQLGGTLTG-IGLAFLLAAGSEAP 257 Query: 264 VFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMANQMLSGLTYALFLSAGV 323 + +I +FG V +L + LRW EP + ++ GL+ L + A + Sbjct: 258 FMKMTDGLIHRFGMVPLLMFATAVSCLRWLFYFFEPPLFFVYMTTIVQGLSVGLSIPAAL 317 Query: 324 NYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVER 364 Y D + + + TA SL+ V + + +GG+++ER Sbjct: 318 QYVRDVAPKQVRATAVSLYSAVGNGLGVWFCTFIGGYMLER 358 >P39589.1 YWBF_BACSU Uncharacterized transporter ywbF Length = 399 Score = 146 bits (368), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 108/383 (28%), Positives = 180/383 (46%), Gaps = 19/383 (4%) Query: 6 RKYS-LHVYNYFFYIAYASYLPFIS-YWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGML 63 R Y+ L + F ++ S +P +S Y E LS Q+GLI S+GP+V QP WGML Sbjct: 8 RHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGML 67 Query: 64 IDYYGMAKWLLLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRH 123 DY K LL + T IT + +AY A F L+I+I+ A F S +P+ D++++R+ Sbjct: 68 SDYTQKTKGLLAVCTSITGIIGLAY-IAFDSFPLFILIAACFAAFQSTIIPLSDSISLRY 126 Query: 124 AKHNSLSYGAIRVVGSISFGLAVAVFGQMYEH-FGISTVFAAYILTMTLVCLLTFSIPGD 182 + + +YG IR+ GS+ FG+AV GQ+ + I +F + + +L +PG Sbjct: 127 TQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILASQVPGQ 186 Query: 183 RKGRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHF 242 +K + + G L+ F+ F+I F NN ++S+++ Sbjct: 187 QK-----------TTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKS 235 Query: 243 GGEASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFE 302 G SA IG+L+ ++EIP F+ I K G + ++ RW + PS Sbjct: 236 GASLSA-IGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLW 294 Query: 303 ILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVV 362 I+ A L G+ LF+ A + Y + + TA +++ + + GG++ Sbjct: 295 IIYATVFLQGVAIGLFIPAALQYVKKITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIF 354 Query: 363 ERGGYSLLFQGAAVMSLLGAAGF 385 + Y +F + +L AGF Sbjct: 355 D---YVSIFAVYLLFGILSIAGF 374 >C0ZL72.1 C0ZL72_BREBN Probable maltose permease Length = 406 Score = 145 bits (367), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 96/359 (26%), Positives = 180/359 (50%), Gaps = 12/359 (3%) Query: 6 RKYSLHVYNYFFYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGMLID 65 R SL F Y + ++ P I+ ++ L+ QIGLI ++ P+V + QP+WGM+ D Sbjct: 16 RYVSLCALYLFIYYGFGAFAPLITQYYESIHLTGTQIGLISAVAPIVSIVAQPMWGMICD 75 Query: 66 YYGMAKWLLLISTGITPWVVIAYRFAGHHFWLYI-VISVVLAVFSSATLPVIDAVTVRHA 124 Y + K +L+++ IT ++ F G ++Y+ ++ ++L+ F SA +P+ D++ + +A Sbjct: 76 RYQIRKTVLILTLIITG--LVGLLFTGVSSFVYVFLLYILLSFFQSAVVPISDSLALGYA 133 Query: 125 KHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRK 184 + + +G IR+ G+I F LA + G + E +G +F ++ L + L +P Sbjct: 134 NGHGIQFGDIRLWGAIGFALAAFITGLLVEQWGPHVLFYSFCLAFLIAILFLRKVP---- 189 Query: 185 GRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFGG 244 E+ R M AG+ L + P+F FLI F NN ++S++ GG Sbjct: 190 ----EEVTEASRFRVSMLAGIRNLARIPRFALFLISSFCMMGSVNANNIWFSLFYQDIGG 245 Query: 245 EASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEIL 304 A IGL + + +E P +GYI+R++G L ++ +RW+ S PS + Sbjct: 246 TI-AGIGLAFLLFAGSEAPFMKLAGYIVRRWGLELTLLLSAIFCTMRWFWYSSAPSTTAV 304 Query: 305 MANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVE 363 +A + G++ +L+ + +N+ + TA ++F V + + + V GW+ + Sbjct: 305 IAMFFVQGISVGFYLATAAQFVRENTPASLQVTALAIFFSVGGGLGSMFCNLVAGWIKD 363 >B1YKG6.1 B1YKG6_EXIS2 Major facilitator superfamily MFS_1 Length = 381 Score = 136 bits (343), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 106/400 (26%), Positives = 190/400 (47%), Gaps = 29/400 (7%) Query: 3 ISYRKYSLHVYNYFFYIAYASYLPFISYWFAEDG--LSTQQIGLIFSIGPLVGFLVQPLW 60 I +KY +F + + +P+++ +F+ D LS +IG I ++GP++ ++QP+W Sbjct: 2 IHRQKYHFQALYFFIFFGQGALIPYLALYFSNDAFQLSASEIGTIVAVGPVLSIVLQPIW 61 Query: 61 GMLIDYYGMAKWLLLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVT 120 GM D +G K LLL++ + I+Y + +L ++IS + A+F A +P++D + Sbjct: 62 GMAADRFGRPKRLLLVALLTAGLLTISYLLTSIYLFL-LLISSLWALFQCAHIPLVDTLA 120 Query: 121 VRHAKHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIP 180 + +K S+ YGA+R+ GS F LAV + GQ+ E + +F A + L L+ I Sbjct: 121 IEFSKRKSVDYGALRLFGSAGFALAVFILGQVTEQGSLGAIFYASGTALVLGFLVLIGI- 179 Query: 181 GDRKGRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLI-------PVFLAAIGPQMNNA 233 + P + + P L +F+ FL P+F NN Sbjct: 180 ---EETPVSTVEREHIPLKA-------LFSNKRFLLFLAGGSLVFGPIF-------ANNY 222 Query: 234 FYSVYISHFGGEASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWY 293 ++ Y++ GE++A +G L+ A L EIP + ++ +FG + +L +S+ ALR Sbjct: 223 YFGSYVT-IRGESTALVGTLFFVAVLCEIPFMRIATRVMMRFGPIPVLVFISVLSALRTG 281 Query: 294 VLSLEPSFEILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIV 353 +L+LEP L L G L + ++Y TA S ++ T +A + Sbjct: 282 ILALEPPIFTLWILAALQGFIIGLLIPVALDYVRSLVPPSTVATAVSTYMATTTGLATAL 341 Query: 354 ASNVGGWVVERGGYSLLFQGAAVMSLLGAAGFASLGRVKR 393 + + G V+++ +F SLLGA + ++K Sbjct: 342 FNLMSGIVLDQWTIYAVFWVYTGSSLLGACLYLISSKIKE 381 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,353,570,826 Number of extensions: 100598575 Number of successful extensions: 384145 Number of sequences better than 1.0e-05: 913 Number of HSP's gapped: 383537 Number of HSP's successfully gapped: 914 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)