BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0095.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4E7P6.1 A4E7P6_9ACTN Putative uncharacterized protein 239 1e-61 C0D1G9.1 C0D1G9_9CLOT Putative uncharacterized protein 224 6e-57 Q2BFE0.1 Q2BFE0_9BACI Putative kinase 223 1e-56 C0BD42.1 C0BD42_9FIRM Putative uncharacterized protein 204 4e-51 B6I2T2.1 B6I2T2_ECOSE Putative kinase 193 1e-47 >A4E7P6.1 A4E7P6_9ACTN Putative uncharacterized protein Length = 266 Score = 239 bits (611), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 128/264 (48%), Positives = 173/264 (65%), Gaps = 5/264 (1%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 ++ GF C D+Y N+ +YPTGN +D+ IH++R GV S VS VG D++G+ M E L Sbjct: 3 IKACAAGFCCADVYQNIGVFYPTGNGIDWGIHLARMGVSVSAVSVVGKDEYGDKMREALA 62 Query: 61 RENVDVSHLHVEEGDTAIFKMDL-NGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHT 119 E +D+SHL VE+GDT++ M L +G DRVH E + GVME + EDD AF+LEH +IHT Sbjct: 63 AEGLDISHLRVEDGDTSVMLMALKDGVDRVHLEAIDGVMETYRPNEDDRAFILEHDIIHT 122 Query: 120 NLSGNIIEWLPRFRASGVLVVFDFTT---KVNSIPEPILPDVDYAFFSYDQDDTWILEYM 176 +L GN ++ LP + +G VV DF+ E P VDYAF S+++D + +++ Sbjct: 123 DLFGNCLDLLPEWHDAGKTVVMDFSVFSQDPEYACENHFPYVDYAFMSFEEDTPELRDWV 182 Query: 177 KWAQALGPRVVVVTLGEKGSIAYDGQSFFREGILKV-PVVNTVGAGDSFCAGFMYGVIQG 235 + Q LGPRV V TLGEKGSIAYDG+ F+ GI+ VVNTVGAGDS+ AGF GVI G Sbjct: 183 RHVQELGPRVAVATLGEKGSIAYDGERFYECGIVPAEKVVNTVGAGDSYIAGFTKGVIDG 242 Query: 236 RSIPDSLRIGAETAAAVVAKFEPY 259 IP ++ GAE ++ V+ FEPY Sbjct: 243 LDIPACMKAGAELSSRVIGSFEPY 266 >C0D1G9.1 C0D1G9_9CLOT Putative uncharacterized protein Length = 286 Score = 224 bits (571), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 112/262 (42%), Positives = 169/262 (64%), Gaps = 3/262 (1%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 M+VA +G NC+D+Y+ + + YPTGN VD +++ + G+ S++S G+D++G M+E L Sbjct: 23 MKVAAIGDNCIDVYERIGKKYPTGNVVDTGVNIQKLGIPVSVISTTGSDENGVWMVEALT 82 Query: 61 RENVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTN 120 +E +D+SH V +G TAI MD++G DRVH + + GV+EN ++DI F EH ++HT Sbjct: 83 KEGLDLSHFKVGDGATAITYMDMDGLDRVHGDYVEGVLENIVFDDEDIRFAAEHDLVHTA 142 Query: 121 LSGNIIEWLPRFRA-SGVLVVFDFTTKVN-SIPEPILPDVDYAFFSYDQD-DTWILEYMK 177 L G + LP+ +A SG + FD+ +++ + E LP VDY F+SY ++ D +I Y+K Sbjct: 143 LWGKAEDALPKIKALSGAKISFDYADRLDHELVEQTLPYVDYGFYSYHKERDPFIEGYLK 202 Query: 178 WAQALGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQGRS 237 G ++ V T G+KGS+AYDG+ F+ GI +VNTVGAGDSF AGF+Y ++ G Sbjct: 203 DKVDRGMKIAVATFGDKGSLAYDGKQFYEGGIYPAKLVNTVGAGDSFIAGFLYEILMGSD 262 Query: 238 IPDSLRIGAETAAAVVAKFEPY 259 I L GA AA VV+ F P+ Sbjct: 263 IEKCLDTGARVAAKVVSTFNPW 284 >Q2BFE0.1 Q2BFE0_9BACI Putative kinase Length = 260 Score = 223 bits (568), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 104/257 (40%), Positives = 164/257 (63%), Gaps = 1/257 (0%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 M+V VG NCMD+Y + + YP GN V+ +++ G + + + VG D +GE MI+ +R Sbjct: 1 MKVVTVGDNCMDVYKDSGKAYPGGNPVNVAVYLKSLGAEPAYIGWVGTDNYGEEMIKSIR 60 Query: 61 RENVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTN 120 + VD +HL ++EG TA+ + + GNDR + GVM FALT +++ F+ H ++H Sbjct: 61 NKGVDTTHLSIKEGKTAVTDVHMEGNDRRFGDYDEGVMSEFALTGEELEFIAAHQLVHAG 120 Query: 121 LSGNIIEWLPRFRASGVLVVFDFTTKV-NSIPEPILPDVDYAFFSYDQDDTWILEYMKWA 179 L G+ + F+ G+L FDF+ ++ + + + +LP VDY FFSY ++D +I +++K A Sbjct: 121 LWGHAENYFSLFKKKGLLTSFDFSDQLAHKLVQSLLPYVDYPFFSYKENDAFIRQFLKSA 180 Query: 180 QALGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQGRSIP 239 G ++ V TLGEKGS+AYDG+ F+ G++ V+T+GAGDSF AGF YG+++G SI Sbjct: 181 VGKGSKIAVATLGEKGSLAYDGRDFYEYGVVDTDAVDTMGAGDSFIAGFTYGILKGLSIE 240 Query: 240 DSLRIGAETAAAVVAKF 256 L +GA+TAA + F Sbjct: 241 SCLELGAKTAAKTIGYF 257 >C0BD42.1 C0BD42_9FIRM Putative uncharacterized protein Length = 283 Score = 204 bits (520), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 108/275 (39%), Positives = 162/275 (58%), Gaps = 22/275 (8%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 +RVA +G NC+D YD+L YP GN V+ +++ R G +SS AVG D G++MI ++ Sbjct: 5 IRVAAIGDNCIDYYDSLNESYPGGNPVNVAVYIKRLGGESSYTGAVGTDSFGKIMISAIQ 64 Query: 61 RENVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTN 120 + VD SH+ V +G TA+ +D+ DRV + GV+ +F L E DI+F+ +H + T Sbjct: 65 NKGVDTSHVQVLDGKTAVTHVDIVDGDRVFGKYEEGVLADFKLREQDISFIKKHDLAVTG 124 Query: 121 LSGNIIEWLPRFRASGVLVVFDFTTK-VNSIPEPILPDVDYAFFSYDQDD---------- 169 + G I + LP + V FDF K N I E +P V YAFFS+D++ Sbjct: 125 IWGMIEDELPLISKE-IPVAFDFANKFANPIVEKAIPYVTYAFFSFDEESRNEFRQKYHS 183 Query: 170 ----------TWILEYMKWAQALGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVG 219 + E+MK Q GP+V++ TLG+ GSI YDG S++R GI++ VV+T+G Sbjct: 184 MGLKEKENCTEQLKEFMKAMQQKGPKVIIATLGKNGSIGYDGNSWYRFGIIECKVVDTMG 243 Query: 220 AGDSFCAGFMYGVIQGRSIPDSLRIGAETAAAVVA 254 AGDSF AGF+YG++ G ++ DS+ GA+ +A + Sbjct: 244 AGDSFIAGFLYGILNGLNVQDSMEAGAQNSAVTIG 278 >B6I2T2.1 B6I2T2_ECOSE Putative kinase Length = 261 Score = 193 bits (490), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 97/252 (38%), Positives = 160/252 (63%), Gaps = 1/252 (0%) Query: 3 VAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLRRE 62 +A +G NC+DIY L + + GN+V+ ++ +R+G+Q ++ VG D +G + + L R Sbjct: 4 LATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARM 63 Query: 63 NVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTNLS 122 VD+SH+H + G TA +++L+ NDRV + GVM +FAL+E+D A++ ++ ++H + Sbjct: 64 GVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHAAIW 123 Query: 123 GNIIEWLPRFRASGVLVVFDFTTKVN-SIPEPILPDVDYAFFSYDQDDTWILEYMKWAQA 181 G+ + P+ A+G L FDF+ K + S+ + ++P +D+AF S Q+D + MK A Sbjct: 124 GHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHLDFAFASAPQEDEALRLKMKAIVA 183 Query: 182 LGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQGRSIPDS 241 G VV+VTLGE GSIA+DG F+R+ V V++T+GAGDSF AGF+ G G ++P + Sbjct: 184 RGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTLPQA 243 Query: 242 LRIGAETAAAVV 253 + +G AA + Sbjct: 244 MALGTACAAKTI 255 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,599,185,278 Number of extensions: 67640817 Number of successful extensions: 156085 Number of sequences better than 1.0e-05: 541 Number of HSP's gapped: 156615 Number of HSP's successfully gapped: 541 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)