BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0093.p (409 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3A4D2.1 C3A4D2_BACMY Permease, probably tetracycline resistance... 645 0.0 Q24XW9.1 Q24XW9_DESHY Putative uncharacterized protein 634 e-180 B8FU76.1 B8FU76_DESHD Major facilitator superfamily MFS_1 634 e-180 Q97L04.1 Q97L04_CLOAB Permease, probably tetracycline resistance... 634 e-180 C2PW14.1 C2PW14_BACCE Permease, probably tetracycline resistance... 627 e-178 >C3A4D2.1 C3A4D2_BACMY Permease, probably tetracycline resistance protein Length = 446 Score = 645 bits (1663), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/407 (78%), Positives = 349/407 (85%) Query: 1 MFKFRSHKEQDTDQTIDKHALIFGLIAVFLCGIGFSIITPVVPFLVQPYISNPGEQAIVV 60 M KFRSH E +T++ IDKHAL+FGLI+VFLCGIGFSII PVVPFLVQPY SN EQA+VV Sbjct: 36 MSKFRSHNEHNTERVIDKHALLFGLISVFLCGIGFSIIIPVVPFLVQPYTSNSAEQAVVV 95 Query: 61 TLLTSVYAVCVFFAAPVLGALSDKYGRRPLLLICLLXXXXXXXXXXXXXXLWVLFAGRII 120 TLLTSVYAVCVFFAAP LGALSDKYGRRPLLL+CL LW+LFAGRII Sbjct: 96 TLLTSVYAVCVFFAAPALGALSDKYGRRPLLLVCLFGSAIGYLVFGIGGALWILFAGRII 155 Query: 121 EGVTGGSISTIFAYFADIIPAEQRTKYFGWVSAVVGAGTVIGPTLGGLLARFGYAVPMYF 180 EG+TGGSISTIFAYFADIIP EQRTKYFGWVSAVVG GTVIGPT+GG LA+FGY+VPMYF Sbjct: 156 EGITGGSISTIFAYFADIIPPEQRTKYFGWVSAVVGVGTVIGPTIGGYLAKFGYSVPMYF 215 Query: 181 GAIITLLNVVYGFFFMPESLDKNHRLKEIALVRLNPFTQLANILSMKSLQRLLVSAFLLW 240 GAIITLLNVVYG +MPESLDKN+RLKEI VRLNPFTQLANILSMK+L+ LLVSAFLLW Sbjct: 216 GAIITLLNVVYGILYMPESLDKNNRLKEITFVRLNPFTQLANILSMKNLKWLLVSAFLLW 275 Query: 241 IPNGSLQAVFSQFTMDTFNWNPALIGLMFSIMGFQDILSQGLVMPKLLKKLSDQQIAVLG 300 IPNGSLQA+FSQFTMDTF+W PALIGLMFSIMGFQDI+SQ +MPKLL KL+D+QIA+LG Sbjct: 276 IPNGSLQAIFSQFTMDTFSWKPALIGLMFSIMGFQDIISQSFIMPKLLTKLTDKQIAILG 335 Query: 301 MVSEMIGYSLIALSALFSFYPLLIAGMFIFGFGDSVFGPSFNGMLSKSVDXXXXXXXXXX 360 MVSE+IGYSLIA SA FSFYP LIAGMFIFGFGDS+FGPSFNGMLSKSVD Sbjct: 336 MVSEIIGYSLIAASAFFSFYPFLIAGMFIFGFGDSIFGPSFNGMLSKSVDSSEQGRIQGG 395 Query: 361 XXXXXALARMLGPILGGQIYVSLGHAAPAVMGMILIGAALPVLYKRT 407 ALARM+GPI+GGQIYVSLGHAAPA MG+ILI AA+ VLYK T Sbjct: 396 SQSVQALARMIGPIIGGQIYVSLGHAAPACMGIILIVAAITVLYKGT 442 >Q24XW9.1 Q24XW9_DESHY Putative uncharacterized protein Length = 411 Score = 634 bits (1635), Expect = e-180, Method: Compositional matrix adjust. Identities = 316/406 (77%), Positives = 348/406 (85%) Query: 1 MFKFRSHKEQDTDQTIDKHALIFGLIAVFLCGIGFSIITPVVPFLVQPYISNPGEQAIVV 60 MFK S EQ+T+QTIDK AL+FGL++VFLCGIGFSIITPVVPFLVQPYISNP EQAI V Sbjct: 1 MFKNGSDHEQNTEQTIDKKALLFGLMSVFLCGIGFSIITPVVPFLVQPYISNPEEQAIAV 60 Query: 61 TLLTSVYAVCVFFAAPVLGALSDKYGRRPLLLICLLXXXXXXXXXXXXXXLWVLFAGRII 120 TLLTSVYA CVF AAPVLGALSD+YGRRPLLLICLL LWVLFAGRII Sbjct: 61 TLLTSVYAACVFLAAPVLGALSDRYGRRPLLLICLLGSAIGYLVFGIGGALWVLFAGRII 120 Query: 121 EGVTGGSISTIFAYFADIIPAEQRTKYFGWVSAVVGAGTVIGPTLGGLLARFGYAVPMYF 180 EG+TGGSI TIFAYFADIIP +QRTKYFGWVSAVVG GTVIGPTLGGLLA+FGY+VPMYF Sbjct: 121 EGITGGSIGTIFAYFADIIPPQQRTKYFGWVSAVVGVGTVIGPTLGGLLAKFGYSVPMYF 180 Query: 181 GAIITLLNVVYGFFFMPESLDKNHRLKEIALVRLNPFTQLANILSMKSLQRLLVSAFLLW 240 G +ITLLNVVYG F+MPESLDKN RL+EI+L+RLNPF QLANILSMKSL RLLVSAFLLW Sbjct: 181 GTVITLLNVVYGLFYMPESLDKNKRLQEISLIRLNPFIQLANILSMKSLTRLLVSAFLLW 240 Query: 241 IPNGSLQAVFSQFTMDTFNWNPALIGLMFSIMGFQDILSQGLVMPKLLKKLSDQQIAVLG 300 +PNGSLQAVFSQFT+DTF+W PALIGLMFSIMG QDI++QG +MPKLL K SDQQIA+LG Sbjct: 241 LPNGSLQAVFSQFTLDTFHWQPALIGLMFSIMGLQDIIAQGFIMPKLLLKFSDQQIAMLG 300 Query: 301 MVSEMIGYSLIALSALFSFYPLLIAGMFIFGFGDSVFGPSFNGMLSKSVDXXXXXXXXXX 360 M +E+IGYS IA SALF FYPLLIAGMFIFGFGDS+FGPSFNGMLSKSV Sbjct: 301 MAAEIIGYSFIAASALFVFYPLLIAGMFIFGFGDSIFGPSFNGMLSKSVPSGEQGRIQGG 360 Query: 361 XXXXXALARMLGPILGGQIYVSLGHAAPAVMGMILIGAALPVLYKR 406 +LARM+GP++GGQIYVSLGHAAPA MGM+LI AA+PVL+KR Sbjct: 361 SQSIQSLARMMGPLIGGQIYVSLGHAAPAFMGMLLIAAAIPVLHKR 406 >B8FU76.1 B8FU76_DESHD Major facilitator superfamily MFS_1 Length = 411 Score = 634 bits (1635), Expect = e-180, Method: Compositional matrix adjust. Identities = 316/406 (77%), Positives = 348/406 (85%) Query: 1 MFKFRSHKEQDTDQTIDKHALIFGLIAVFLCGIGFSIITPVVPFLVQPYISNPGEQAIVV 60 MFK S EQ+T+QTIDK AL+FGL++VFLCGIGFSIITPVVPFLVQPYISNP EQAI V Sbjct: 1 MFKNGSDHEQNTEQTIDKKALLFGLMSVFLCGIGFSIITPVVPFLVQPYISNPEEQAIAV 60 Query: 61 TLLTSVYAVCVFFAAPVLGALSDKYGRRPLLLICLLXXXXXXXXXXXXXXLWVLFAGRII 120 TLLTSVYA CVF AAPVLGALSD+YGRRPLLLICLL LWVLFAGRII Sbjct: 61 TLLTSVYAACVFLAAPVLGALSDRYGRRPLLLICLLGSAIGYLVFGIGGALWVLFAGRII 120 Query: 121 EGVTGGSISTIFAYFADIIPAEQRTKYFGWVSAVVGAGTVIGPTLGGLLARFGYAVPMYF 180 EG+TGGSI TIFAYFADIIP +QRTKYFGWVSAVVG GTVIGPTLGGLLA+FGY+VPMYF Sbjct: 121 EGITGGSIGTIFAYFADIIPPQQRTKYFGWVSAVVGVGTVIGPTLGGLLAKFGYSVPMYF 180 Query: 181 GAIITLLNVVYGFFFMPESLDKNHRLKEIALVRLNPFTQLANILSMKSLQRLLVSAFLLW 240 G +ITLLNVVYG F+MPESLDKN RL+EI+L+RLNPF QLANILSMKSL RLLVSAFLLW Sbjct: 181 GTVITLLNVVYGLFYMPESLDKNKRLQEISLIRLNPFIQLANILSMKSLTRLLVSAFLLW 240 Query: 241 IPNGSLQAVFSQFTMDTFNWNPALIGLMFSIMGFQDILSQGLVMPKLLKKLSDQQIAVLG 300 +PNGSLQAVFSQFT+DTF+W PALIGLMFSIMG QDI++QG +MPKLL K SDQQIA+LG Sbjct: 241 LPNGSLQAVFSQFTLDTFHWQPALIGLMFSIMGLQDIIAQGFIMPKLLLKFSDQQIAMLG 300 Query: 301 MVSEMIGYSLIALSALFSFYPLLIAGMFIFGFGDSVFGPSFNGMLSKSVDXXXXXXXXXX 360 M +E+IGYS IA SALF FYPLLIAGMFIFGFGDS+FGPSFNGMLSKSV Sbjct: 301 MAAEIIGYSFIAASALFVFYPLLIAGMFIFGFGDSIFGPSFNGMLSKSVPSGEQGRIQGG 360 Query: 361 XXXXXALARMLGPILGGQIYVSLGHAAPAVMGMILIGAALPVLYKR 406 +LARM+GP++GGQIYVSLGHAAPA MGM+LI AA+PVL+KR Sbjct: 361 SQSIQSLARMMGPLIGGQIYVSLGHAAPAFMGMLLIAAAIPVLHKR 406 >Q97L04.1 Q97L04_CLOAB Permease, probably tetracycline resistance protein Length = 411 Score = 634 bits (1635), Expect = e-180, Method: Compositional matrix adjust. Identities = 310/406 (76%), Positives = 351/406 (86%) Query: 1 MFKFRSHKEQDTDQTIDKHALIFGLIAVFLCGIGFSIITPVVPFLVQPYISNPGEQAIVV 60 M KF+SH +T QTI+KHALIFGLI+VFL GIGF+II+PV+PFLVQPYI+NPG QAI+V Sbjct: 1 MSKFKSHNSNNTKQTINKHALIFGLISVFLSGIGFTIISPVIPFLVQPYINNPGNQAIIV 60 Query: 61 TLLTSVYAVCVFFAAPVLGALSDKYGRRPLLLICLLXXXXXXXXXXXXXXLWVLFAGRII 120 TLLTSVYA+C+FF+AP LGALSDKYGRRP+LL+CLL LWVLFAGRII Sbjct: 61 TLLTSVYAICMFFSAPGLGALSDKYGRRPVLLVCLLGSSIGYLIFGIGGALWVLFAGRII 120 Query: 121 EGVTGGSISTIFAYFADIIPAEQRTKYFGWVSAVVGAGTVIGPTLGGLLARFGYAVPMYF 180 +G+TGG+ISTIFAYFADIIP +RTKYFGWVSAVVG GT+IGPTLGGLLA+FGY+VP+YF Sbjct: 121 DGITGGTISTIFAYFADIIPENERTKYFGWVSAVVGVGTIIGPTLGGLLAKFGYSVPLYF 180 Query: 181 GAIITLLNVVYGFFFMPESLDKNHRLKEIALVRLNPFTQLANILSMKSLQRLLVSAFLLW 240 GAIITLLNVVYGFFFMPESLDKN RLKEI VRLNPFTQLAN+LS+KSL+RLL+SAFLLW Sbjct: 181 GAIITLLNVVYGFFFMPESLDKNIRLKEITFVRLNPFTQLANLLSIKSLKRLLISAFLLW 240 Query: 241 IPNGSLQAVFSQFTMDTFNWNPALIGLMFSIMGFQDILSQGLVMPKLLKKLSDQQIAVLG 300 IPNGSLQAVFSQFT+DTF+W PA+IGLMFSIMGFQDI+SQ L+MPKLL KLSD+ IA+LG Sbjct: 241 IPNGSLQAVFSQFTIDTFSWKPAIIGLMFSIMGFQDIISQSLIMPKLLVKLSDKYIAILG 300 Query: 301 MVSEMIGYSLIALSALFSFYPLLIAGMFIFGFGDSVFGPSFNGMLSKSVDXXXXXXXXXX 360 M+SE+IGYSLIA SALF FYPL IAGMFIFGFGDS+FGPSFNGMLSKSVD Sbjct: 301 MISEVIGYSLIAASALFYFYPLFIAGMFIFGFGDSIFGPSFNGMLSKSVDSSEQGRIQGG 360 Query: 361 XXXXXALARMLGPILGGQIYVSLGHAAPAVMGMILIGAALPVLYKR 406 ALARM+GPI+GGQIYVSLGHA PA MG++LI A+PVLYK Sbjct: 361 SQSIQALARMIGPIIGGQIYVSLGHATPAFMGIVLITVAIPVLYKN 406 >C2PW14.1 C2PW14_BACCE Permease, probably tetracycline resistance protein Length = 411 Score = 627 bits (1616), Expect = e-178, Method: Compositional matrix adjust. Identities = 312/404 (77%), Positives = 346/404 (85%) Query: 4 FRSHKEQDTDQTIDKHALIFGLIAVFLCGIGFSIITPVVPFLVQPYISNPGEQAIVVTLL 63 FRS E +T++ IDKHALIFGLI+VFLCGIGFSII PVVPFLVQPYIS+P EQA+VVTLL Sbjct: 4 FRSQNESETEKNIDKHALIFGLISVFLCGIGFSIIMPVVPFLVQPYISSPEEQALVVTLL 63 Query: 64 TSVYAVCVFFAAPVLGALSDKYGRRPLLLICLLXXXXXXXXXXXXXXLWVLFAGRIIEGV 123 TSVYAVCVFFAAPVLGALSDKYGRRPLLLICL LWVLFAGRIIEG+ Sbjct: 64 TSVYAVCVFFAAPVLGALSDKYGRRPLLLICLFGSAIGYLVFGIGGALWVLFAGRIIEGI 123 Query: 124 TGGSISTIFAYFADIIPAEQRTKYFGWVSAVVGAGTVIGPTLGGLLARFGYAVPMYFGAI 183 TGGSISTIFAYFADIIP EQRTKYFGWVSAVVG GT+IGPTLGGLLA+FG++VP+YFGA+ Sbjct: 124 TGGSISTIFAYFADIIPPEQRTKYFGWVSAVVGVGTIIGPTLGGLLAKFGHSVPLYFGAL 183 Query: 184 ITLLNVVYGFFFMPESLDKNHRLKEIALVRLNPFTQLANILSMKSLQRLLVSAFLLWIPN 243 ITLLNV+YG +MPESLDKN+RLKEI VRLNPF QLANILSMK+L+ LLVSAFLLWIPN Sbjct: 184 ITLLNVLYGMKYMPESLDKNNRLKEITFVRLNPFAQLANILSMKNLKWLLVSAFLLWIPN 243 Query: 244 GSLQAVFSQFTMDTFNWNPALIGLMFSIMGFQDILSQGLVMPKLLKKLSDQQIAVLGMVS 303 GSLQA+F+QFTMDTF+W PALIGLMFSIMGFQ+I+SQ +MPKLL KLSD+QIA+LGMVS Sbjct: 244 GSLQAIFTQFTMDTFSWKPALIGLMFSIMGFQEIISQAFIMPKLLIKLSDKQIAILGMVS 303 Query: 304 EMIGYSLIALSALFSFYPLLIAGMFIFGFGDSVFGPSFNGMLSKSVDXXXXXXXXXXXXX 363 E+IGYS IA SALFS YPLLI GM +FGFGDS+FGPSFNGMLSKSVD Sbjct: 304 EIIGYSFIAASALFSLYPLLIIGMLMFGFGDSIFGPSFNGMLSKSVDSSEQGRIQGGSQS 363 Query: 364 XXALARMLGPILGGQIYVSLGHAAPAVMGMILIGAALPVLYKRT 407 ALARM+GPI+GGQIYV+LGHAAPA MGMILI A+ VLY+ T Sbjct: 364 IQALARMIGPIIGGQIYVTLGHAAPAFMGMILIVVAIAVLYRGT 407 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.145 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,226,685,504 Number of extensions: 87734866 Number of successful extensions: 376825 Number of sequences better than 1.0e-05: 4085 Number of HSP's gapped: 377487 Number of HSP's successfully gapped: 4121 Length of query: 409 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 271 Effective length of database: 1,760,596,133 Effective search space: 477121552043 Effective search space used: 477121552043 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)