BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0088.p (285 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJX6.1 B3KJX6_9BACI Xylose isomerase domain protein TIM barrel 391 e-107 B1D6I2.1 B1D6I2_9BACL Xylose isomerase domain protein TIM barrel 365 3e-99 B3KI52.1 B3KI52_9BACI Xylose isomerase domain protein TIM barrel 303 9e-81 B1D736.1 B1D736_9BACL Xylose isomerase domain protein TIM barrel 299 2e-79 A3I7X6.1 A3I7X6_9BACI Putative uncharacterized protein 283 1e-74 >B3KJX6.1 B3KJX6_9BACI Xylose isomerase domain protein TIM barrel Length = 285 Score = 391 bits (1004), Expect = e-107, Method: Compositional matrix adjust. Identities = 184/283 (65%), Positives = 217/283 (76%) Query: 1 MKLGISTYSLHSAFAAGELTLKGVLETIADLGAEHAEIVPLGFNLIDNPELVGVILQTAA 60 MKLGIS+YSL+ A GE+T+ V++ +A++G EH EIVPLGFNL + PEL+ I AA Sbjct: 1 MKLGISSYSLYQAMNKGEMTITDVIDYVAEIGGEHIEIVPLGFNLKETPELIDTIKSRAA 60 Query: 61 DRGLELSNYAIGANFAGXXXXXXXXXXXXXXXXXDVCAALGIQRMRHDVASSPDLSIVRF 120 +GLE+SNYAIGANFA D+ AALG++ MRHDV SS DLSI F Sbjct: 61 SKGLEISNYAIGANFAELDDAAYEAEIERVKGEVDIAAALGVKLMRHDVGSSKDLSITHF 120 Query: 121 LEELPQLAEACREIADYASGFGITTSVENHGYFIQHSDRVQALVNAVGRDNFRTTLDVGN 180 LEELP+LA ACR IADYA+ GITTSVENHGYFIQHSDRV+ALV V NF+TTLD+GN Sbjct: 121 LEELPRLANACRIIADYAAERGITTSVENHGYFIQHSDRVKALVQTVNHPNFKTTLDIGN 180 Query: 181 FLCADENPLIAVASNLKLASMVHLKDFYIRPQDRHPGEGWFQSSGGNWLRGAIVGQGDID 240 FLCADE +AVA+N+ LASMVH KDFY RP +R PG GWF +S GNWLRGAIVGQGDID Sbjct: 181 FLCADEVSTVAVANNIGLASMVHFKDFYYRPANRAPGSGWFNTSSGNWLRGAIVGQGDID 240 Query: 241 MPRVLQLVKESGYDGYISIEFEGMEECRKGTRLGLEYVKRTWN 283 MP V+++VKESGYDGYISIEFEGME+CR G + GLEYV++ WN Sbjct: 241 MPEVVRIVKESGYDGYISIEFEGMEDCRTGAKFGLEYVRKAWN 283 >B1D6I2.1 B1D6I2_9BACL Xylose isomerase domain protein TIM barrel Length = 285 Score = 365 bits (936), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 172/285 (60%), Positives = 215/285 (75%) Query: 1 MKLGISTYSLHSAFAAGELTLKGVLETIADLGAEHAEIVPLGFNLIDNPELVGVILQTAA 60 MK GISTYS + A +GE+ + V+ IA++G EH EIVPLGFNL +NPEL+ I + A Sbjct: 1 MKFGISTYSYYQALQSGEMDVIDVIAAIAEIGGEHVEIVPLGFNLTENPELIEAIRRKAD 60 Query: 61 DRGLELSNYAIGANFAGXXXXXXXXXXXXXXXXXDVCAALGIQRMRHDVASSPDLSIVRF 120 + G+++SNY IGANFAG D+ A LG+Q MRHDVASSPD SI F Sbjct: 61 EVGIDISNYLIGANFAGKSEAEFEQEIERVMREVDIAAKLGVQLMRHDVASSPDTSIRNF 120 Query: 121 LEELPQLAEACREIADYASGFGITTSVENHGYFIQHSDRVQALVNAVGRDNFRTTLDVGN 180 ELP+LAEACR IADYA+ GITT+VENHGYF+Q SDRVQAL++A RDNFRTTLDVGN Sbjct: 121 YNELPKLAEACRRIADYAATKGITTTVENHGYFLQASDRVQALLHATDRDNFRTTLDVGN 180 Query: 181 FLCADENPLIAVASNLKLASMVHLKDFYIRPQDRHPGEGWFQSSGGNWLRGAIVGQGDID 240 F+C DENP++ V++N++ A+MVH+KDFY+R + G+GWF+S+ GN+LRGAIVGQGDID Sbjct: 181 FVCVDENPVVGVSNNIEYAAMVHIKDFYVRHPESELGDGWFRSTRGNYLRGAIVGQGDID 240 Query: 241 MPRVLQLVKESGYDGYISIEFEGMEECRKGTRLGLEYVKRTWNAL 285 M RVL +VKESGYDGYISIEFEG+E CR G + GL +K+ W +L Sbjct: 241 MRRVLGIVKESGYDGYISIEFEGLEPCRFGVQQGLNQLKKIWQSL 285 >B3KI52.1 B3KI52_9BACI Xylose isomerase domain protein TIM barrel Length = 290 Score = 303 bits (777), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 150/287 (52%), Positives = 200/287 (69%), Gaps = 5/287 (1%) Query: 1 MKLGISTYSLHSAFAAGELTLKGVLETIADLGAEHAEIVPLGFNLIDNPELVGVILQTAA 60 M++G+STYSL A AGE+T+ V++ IA+ G +H EIVP GF L DNP L + + AA Sbjct: 1 MRVGVSTYSLLPAIRAGEMTVLDVVDWIAENGGQHMEIVPYGFTLEDNPGLADAVRERAA 60 Query: 61 DRGLELSNYAIGANFAGXXXXXXXXXXXXXXXXXDVCAALGIQRMRHDVAS---SPDLSI 117 + G+ LSNY++ ANF D LG++ MRHDV + P+ Sbjct: 61 EAGIALSNYSMPANFVQESRDDFDAEVERIKRHVDTVNRLGMKHMRHDVTAFTLPPEKMG 120 Query: 118 VRFLE-ELPQLAEACREIADYASGFGITTSVENHGYFIQHSDRVQALVNAVGRDNFRTTL 176 + ++E L + E CR+IADYA+ GITT++ENHG +Q SDRVQ ++ AV R NF+TTL Sbjct: 121 IDYVETHLDMIVEGCRQIADYAARSGITTTIENHGVSVQASDRVQRVLQAVDRPNFKTTL 180 Query: 177 DVGNFLCADENPLIAVASNLKLASMVHLKDFYIRPQDRHPGEG-WFQSSGGNWLRGAIVG 235 D+GNFLC DE PL+ V NL LAS+VH+KDFY RP D+ PG G WF++S GN+LRG+I G Sbjct: 181 DIGNFLCVDEQPLVGVKRNLPLASLVHVKDFYYRPYDQDPGGGKWFRTSHGNYLRGSIFG 240 Query: 236 QGDIDMPRVLQLVKESGYDGYISIEFEGMEECRKGTRLGLEYVKRTW 282 QGD+D+ R+L+L+KESGYDG I++EFEGMEECR+GTRLGL+ +KR W Sbjct: 241 QGDLDVRRILRLIKESGYDGDITLEFEGMEECREGTRLGLDNLKRLW 287 >B1D736.1 B1D736_9BACL Xylose isomerase domain protein TIM barrel Length = 290 Score = 299 bits (766), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 142/290 (48%), Positives = 196/290 (67%), Gaps = 5/290 (1%) Query: 1 MKLGISTYSLHSAFAAGELTLKGVLETIADLGAEHAEIVPLGFNLIDNPELVGVILQTAA 60 MK+G+STYSL A AG++++ V+E IA G EH EIVP GF L+D PEL + A Sbjct: 1 MKVGLSTYSLLPALKAGDMSVLDVVEWIAGNGGEHMEIVPYGFTLVDQPELADAVRDKAK 60 Query: 61 DRGLELSNYAIGANFAGXXXXXXXXXXXXXXXXXDVCAALGIQRMRHDVAS----SPDLS 116 G+ELSNY++ ANF D+ LG++ MRHDV + + +++ Sbjct: 61 IAGIELSNYSMPANFVQGSEEAFQAEVARVKEHVDLVNRLGMKHMRHDVTAFTLPTENMT 120 Query: 117 IVRFLEELPQLAEACREIADYASGFGITTSVENHGYFIQHSDRVQALVNAVGRDNFRTTL 176 I F E L + + R IADYA+ +GITT++ENHG+ +Q SDRV +++ V R NF+TTL Sbjct: 121 IQWFEENLHHMVKGSRLIADYAAAYGITTTIENHGFSVQASDRVLRVLHEVNRPNFKTTL 180 Query: 177 DVGNFLCADENPLIAVASNLKLASMVHLKDFYIRPQDRHPGEG-WFQSSGGNWLRGAIVG 235 DVGNFLC DENPL+ V+ NL AS++H+KDFYIRP D PG G WF+S+ G +LRGAI G Sbjct: 181 DVGNFLCVDENPLVGVSRNLPYASLIHIKDFYIRPSDESPGGGDWFKSAHGTYLRGAIAG 240 Query: 236 QGDIDMPRVLQLVKESGYDGYISIEFEGMEECRKGTRLGLEYVKRTWNAL 285 GD+D+ R+ +L+K+SGYDGY+++EFEGMEECR +R+ ++ VKR W + Sbjct: 241 HGDLDLRRIFRLIKKSGYDGYLTVEFEGMEECRMASRIAMDNVKRLWQEV 290 >A3I7X6.1 A3I7X6_9BACI Putative uncharacterized protein Length = 292 Score = 283 bits (725), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 134/288 (46%), Positives = 189/288 (65%), Gaps = 5/288 (1%) Query: 1 MKLGISTYSLHSAFAAGELTLKGVLETIADLGAEHAEIVPLGFNLIDNPELVGVILQTAA 60 MK+G+STYS+ GE+++ VL+ +AD G +H E+VP F ++DN EL I + AA Sbjct: 1 MKVGLSTYSMVKKMKQGEMSVLDVLQWVADNGGQHVELVPYEFTVVDNDELATQIKEKAA 60 Query: 61 DRGLELSNYAIGANFAGXXXXXXXXXXXXXXXXXDVCAALGIQRMRHDVA----SSPDLS 116 + GLELS Y++ ANF DV +GI+ MRHDV + Sbjct: 61 ELGLELSAYSLPANFIHETEEAFFEEVERLKKHVDVVHRMGIKIMRHDVTLFTLPKEQTT 120 Query: 117 IVRFLEELPQLAEACREIADYASGFGITTSVENHGYFIQHSDRVQALVNAVGRDNFRTTL 176 I + E ++ C+ +ADYA F ITT++ENHG+ +Q SDRVQ L+ AV RDNF+TTL Sbjct: 121 IHYYNEHFDEMVRGCQLLADYAKQFNITTTIENHGFNVQTSDRVQQLIQAVNRDNFKTTL 180 Query: 177 DVGNFLCADENPLIAVASNLKLASMVHLKDFYIRPQDRHPGEG-WFQSSGGNWLRGAIVG 235 D+GNFLC DE PLI V N+ A+ VH KDFYIRP +PG+G WF++ N++RGAIVG Sbjct: 181 DIGNFLCIDEEPLIGVMKNISYAATVHFKDFYIRPYFENPGDGEWFRTVHNNYIRGAIVG 240 Query: 236 QGDIDMPRVLQLVKESGYDGYISIEFEGMEECRKGTRLGLEYVKRTWN 283 GDI++ +++LVK GYDGYI++EFEGME+C+ G+++G++ V+R W+ Sbjct: 241 HGDINIREIIKLVKGYGYDGYITVEFEGMEDCQVGSKIGMDNVRRLWD 288 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,608,074,058 Number of extensions: 63758053 Number of successful extensions: 210679 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 210764 Number of HSP's successfully gapped: 33 Length of query: 285 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 151 Effective length of database: 1,798,280,193 Effective search space: 271540309143 Effective search space used: 271540309143 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)