BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0080.p (496 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65MD8.1 Q65MD8_BACLD SacX 314 2e-83 B7GMN6.1 B7GMN6_ANOFW Phosphotransferase system (PTS) sucrose-sp... 313 2e-83 P15400.1 SACX_BACSU Negative regulator of sacY activity Phosphot... 311 1e-82 C3IZD0.1 C3IZD0_9BACI PTS system, sucrose-specific IIBC subunit 309 5e-82 B7Y761.1 B7Y761_9BACI PTS system, sucrose-specific IIBC subunit 309 5e-82 >Q65MD8.1 Q65MD8_BACLD SacX Length = 458 Score = 314 bits (804), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 175/462 (37%), Positives = 253/462 (54%), Gaps = 13/462 (2%) Query: 24 ELAERLIGLSGGAGNVLEAIHCTTXXXXXXXDSSRVDEAGLSGIQEVQGVFFRTGQLQII 83 E+A L+ L GGA N++ HCTT D ++D A + ++ VQG FFR G QII Sbjct: 5 EIARELLPLIGGAENIISISHCTTRLRFNVRDEEKIDIAAIEKMESVQGTFFRYGLFQII 64 Query: 84 LGSANVFKVHRQVVRILQEGGAKQQPEGTGDLSQKNRQEQQGVLKQIADAVSFFSDIVVP 143 G+ V K+++++++ ++ +++ + VL + A +S DI VP Sbjct: 65 FGTGVVNKIYKEMIQYSDPAPFEE--------ARREARPNMNVLTRFAKTLS---DIFVP 113 Query: 144 MIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTAKRFGGT 203 +IP S+++ F + L + + SAF +L V+ G+ AK+FG Sbjct: 114 IIPAIVASGLLMGLISVLKEFSLGAYGGPLMKMLDIFSSSAFVILPVLIGFSAAKQFGAN 173 Query: 204 PPLGAVIGLVMTHPGLLHVTGFGRAPVSSADV--MIAPQFGYQGAVIPTILAVLVMTLIE 261 P LGAVIG ++THP LL + G S +V + P GYQG VIP +L+V +M+ IE Sbjct: 174 PFLGAVIGGILTHPDLLDPSMLGSQKPESIEVFGLEVPLIGYQGTVIPILLSVYMMSKIE 233 Query: 262 KGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSGGTVFGL 321 K L+R++P+S ++ PF PL+ ++ + L V+ G G Sbjct: 234 KFLKRIVPSSLDLIVAPFITVMASGFAALFIMGPLSLMIAHFISDCLGFVYHFAGACAGF 293 Query: 322 LLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAVYARTRD 381 L GG+Y+ LV+SGLHH IE L+ +P+ VNFLLPIWSMAN+AQG AGLAV+ +T++ Sbjct: 294 LFGGLYALLVLSGLHHSFYVIEASLLADPEYGVNFLLPIWSMANVAQGGAGLAVFLKTKN 353 Query: 382 PALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVAADSFGL 441 +RKIA+PAS+TAFLGI EPV FGVNLKL RPF+ Y F VAA+S+GL Sbjct: 354 DGIRKIAIPASLTAFLGIVEPVMFGVNLKLVRPFIGASIGGALGGAYAVFTHVAANSYGL 413 Query: 442 TGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 +GIPM + + LG N+IHY T LG + Sbjct: 414 SGIPMISIILPLGTANLIHYLIAFAIAVISAFIATLCLGFKD 455 >B7GMN6.1 B7GMN6_ANOFW Phosphotransferase system (PTS) sucrose-specific enzyme IIBC component Length = 456 Score = 313 bits (803), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 178/463 (38%), Positives = 258/463 (55%), Gaps = 15/463 (3%) Query: 24 ELAERLIGLSGGAGNVLEAIHCTTXXXXXXXDSSRVDEAGLSGIQEVQGVFFRTGQLQII 83 + ++L+ L GG NV+ A+HC T D +VD+ + + V+G F +GQ QII Sbjct: 5 HVTKQLLSLLGGKENVISAVHCATRLRLVLKDEKKVDQQAIEQLDGVKGAFSSSGQYQII 64 Query: 84 LGSANVFKVHRQVVRILQEGGAKQQPEGTGDLSQKNRQEQQGVLKQIADAVSFFSDIVVP 143 G+ V KV+ Q V+ E G + + D+ Q+ +++ + ++A +S +I VP Sbjct: 65 FGTGVVNKVYEQFVK---EAGLQNV---SADVHQEAVKQKMNPIARLAKTLS---NIFVP 115 Query: 144 MIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTAKRFGGT 203 +IP MM+ F W DSA L + + +AF +L ++ GY AK FGG Sbjct: 116 IIPAIVASGLLMGLLGMMKAFKWVAPDSAWIVLLDMFSSAAFIILPILIGYSAAKEFGGN 175 Query: 204 PPLGAVIGLVMTHPGLLHVTGFGRAPVSSADVM---IAPQFGYQGAVIPTILAVLVMTLI 260 P LGAVIG +MTHP LL+ G G A + IA GYQG VIP +LA VM+ I Sbjct: 176 PFLGAVIGGIMTHPALLNPWGLGSAEPEYLHFLGFDIA-MIGYQGTVIPILLATYVMSKI 234 Query: 261 EKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSGGTVFG 320 EK LR+++P + ++ PF PL + + + + L V+ +GG + G Sbjct: 235 EKSLRKVVPHAVDLLVTPFITVILTGFTAILVIGPLGNIVGKGITTTLNFVYDTGGALAG 294 Query: 321 LLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAVYARTR 380 L+ GG+YS +V++G+HH AIE GL+ DI N+LLPIWSMAN+AQG AGLAV+ +++ Sbjct: 295 LIFGGLYSMIVITGVHHSFHAIEAGLLA--DIGKNYLLPIWSMANVAQGGAGLAVFLKSK 352 Query: 381 DPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVAADSFG 440 ++IALPA+ +AFLGITEPV FGVNLK +PF+ YV F V A+++G Sbjct: 353 RTKTKEIALPAAFSAFLGITEPVIFGVNLKYRKPFIAAAIGGAVGGAYVVFTNVVANAYG 412 Query: 441 LTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 LTGIPM A +G +N+I+Y TW+LG E Sbjct: 413 LTGIPMIAIVAPMGMVNLINYLIGFAIAVGTAFVATWILGFKE 455 >P15400.1 SACX_BACSU Negative regulator of sacY activity Phosphotransferase enzyme IIB component Permease IIC component EC=2.7.1.69 Length = 459 Score = 311 bits (796), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 180/464 (38%), Positives = 254/464 (54%), Gaps = 11/464 (2%) Query: 24 ELAERLIGLSGGAGNVLEAIHCTTXXXXXXXDSSRVDEAGLSGIQEVQGVFFRTGQLQII 83 E+A+ L+ L+GG N++ HCTT D +++D + +Q VQG FFR G QII Sbjct: 4 EIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQII 63 Query: 84 LGSANVFKVHRQVVRILQEGGAKQQPEGTGDLSQKNRQEQQGVLKQIADAVSFFSDIVVP 143 G+ V K++++VV + E ++P S+K L A SDI VP Sbjct: 64 FGAGVVNKIYKEVVHV-WETAPSEEPVHQKKASRK--------LNPAAAFAKTLSDIFVP 114 Query: 144 MIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTAKRFGGT 203 +IP M++VF W S + L L++ +AF +L ++ G+ A++FG Sbjct: 115 IIPAITASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLPILVGFSAARQFGSN 174 Query: 204 PPLGAVIGLVMTHPGLLHVTGFGRAPVSSADV--MIAPQFGYQGAVIPTILAVLVMTLIE 261 P LGAVI ++THP LL + G SS D+ + P GYQG++IP +L+V VM+ IE Sbjct: 175 PYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIE 234 Query: 262 KGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSGGTVFGL 321 K L+ ++P S ++IPF P S + + + ++ G G Sbjct: 235 KLLKSIVPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGA 294 Query: 322 LLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAVYARTRD 381 L GGIYS++V+SGLHH AIE L+ NP + VNFL+PIWSMAN+AQG AGLAV+ +T+ Sbjct: 295 LFGGIYSTIVLSGLHHSFYAIEATLLANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQ 354 Query: 382 PALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVAADSFGL 441 +L+KIALPAS+TAFLGI EP+ FGVNLKL RPF+ YV QV A+S+GL Sbjct: 355 SSLKKIALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGAYVVAVQVVANSYGL 414 Query: 442 TGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDEPS 485 TGIPM + + G N +HY T LG E + Sbjct: 415 TGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEET 458 >C3IZD0.1 C3IZD0_9BACI PTS system, sucrose-specific IIBC subunit Length = 458 Score = 309 bits (791), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 176/463 (38%), Positives = 255/463 (55%), Gaps = 15/463 (3%) Query: 24 ELAERLIGLSGGAGNVLEAIHCTTXXXXXXXDSSRVDEAGLSGIQEVQGVFFRTGQLQII 83 +A++LI L GG N++ A HC T D + + + I+ V+G F +GQ QII Sbjct: 5 HIAKQLIPLLGGKENIISATHCATRLRLVLKDDKKAEAKAIENIEGVKGAFSSSGQFQII 64 Query: 84 LGSANVFKVHRQVVRILQEGGAKQQPEGTGDLSQKNRQEQQGVLKQIADAVSFFSDIVVP 143 G+ V KV+ R E G +Q G D Q+ +++ L + A +S +I VP Sbjct: 65 FGTGVVNKVYEAFAR---EAGVQQ---GDADAHQEAIKQKMNPLARFAKTLS---NIFVP 115 Query: 144 MIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTAKRFGGT 203 +IP MM+ F W DS + L + + +AF +L ++ G+ AK FGGT Sbjct: 116 IIPAIVASGLLMGLLGMMKAFKWVAADSPLYILLDMFSSAAFIILPILIGFSAAKEFGGT 175 Query: 204 PPLGAVIGLVMTHPGLLHVTGFGRAPVSSADVM---IAPQFGYQGAVIPTILAVLVMTLI 260 P LGAVIG +MTHP LL+ G A + + IA GYQG V+P +LA VM+ + Sbjct: 176 PFLGAVIGGIMTHPALLNPWGLAEAKPNYMHFLGFDIA-MVGYQGTVVPILLATYVMSKV 234 Query: 261 EKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSGGTVFG 320 E+GLRR++P + S +++PF PL + L + ++L V+ GG + G Sbjct: 235 ERGLRRVVPHAVSLLVVPFVTVILTGFITILAIGPLGNLLGDGITTVLNFVYHYGGALAG 294 Query: 321 LLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAVYARTR 380 L+ GG+YS +V++G+HH AIE L+ + VN+LLPIWSMAN+AQG AGLAV+ +++ Sbjct: 295 LIFGGLYSMIVITGVHHSFHAIEANLLA--KLGVNYLLPIWSMANVAQGGAGLAVFVKSK 352 Query: 381 DPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVAADSFG 440 ++IALPA+++AFLGITEPV FGVNLK +PF+ YV F V A+++G Sbjct: 353 RTKTKEIALPAALSAFLGITEPVIFGVNLKYRKPFIAAAIGGALGGAYVVFTNVVANAYG 412 Query: 441 LTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 LTGIPM A G N++HY TW+L E Sbjct: 413 LTGIPMIAIVAPEGLTNLMHYLIGFAIAVVSAFVATWLLKFRE 455 >B7Y761.1 B7Y761_9BACI PTS system, sucrose-specific IIBC subunit Length = 458 Score = 309 bits (791), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 176/463 (38%), Positives = 255/463 (55%), Gaps = 15/463 (3%) Query: 24 ELAERLIGLSGGAGNVLEAIHCTTXXXXXXXDSSRVDEAGLSGIQEVQGVFFRTGQLQII 83 +A++LI L GG N++ A HC T D + + + I+ V+G F +GQ QII Sbjct: 5 HIAKQLIPLLGGKENIISATHCATRLRLVLKDDKKAEAKAIENIEGVKGAFSSSGQFQII 64 Query: 84 LGSANVFKVHRQVVRILQEGGAKQQPEGTGDLSQKNRQEQQGVLKQIADAVSFFSDIVVP 143 G+ V KV+ R E G +Q G D Q+ +++ L + A +S +I VP Sbjct: 65 FGTGVVNKVYEAFAR---EAGVQQ---GDADAHQEAIKQKMNPLARFAKTLS---NIFVP 115 Query: 144 MIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTAKRFGGT 203 +IP MM+ F W DS + L + + +AF +L ++ G+ AK FGGT Sbjct: 116 IIPAIVASGLLMGLLGMMKAFKWVAADSPLYILLDMFSSAAFIILPILIGFSAAKEFGGT 175 Query: 204 PPLGAVIGLVMTHPGLLHVTGFGRAPVSSADVM---IAPQFGYQGAVIPTILAVLVMTLI 260 P LGAVIG +MTHP LL+ G A + + IA GYQG V+P +LA VM+ + Sbjct: 176 PFLGAVIGGIMTHPALLNPWGLAEAKPNYMHFLGFDIA-MVGYQGTVVPILLATYVMSKV 234 Query: 261 EKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSGGTVFG 320 E+GLRR++P + S +++PF PL + L + ++L V+ GG + G Sbjct: 235 ERGLRRVVPHAVSLLVVPFVTVILTGFITILAIGPLGNLLGDGITTVLNFVYHYGGALAG 294 Query: 321 LLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAVYARTR 380 L+ GG+YS +V++G+HH AIE L+ + VN+LLPIWSMAN+AQG AGLAV+ +++ Sbjct: 295 LIFGGLYSMIVITGVHHSFHAIEANLLA--KLGVNYLLPIWSMANVAQGGAGLAVFVKSK 352 Query: 381 DPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVAADSFG 440 ++IALPA+++AFLGITEPV FGVNLK +PF+ YV F V A+++G Sbjct: 353 RTKTKEIALPAALSAFLGITEPVIFGVNLKYRKPFIAAAIGGALGGAYVVFTNVVANAYG 412 Query: 441 LTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 LTGIPM A G N++HY TW+L E Sbjct: 413 LTGIPMIAIVAPEGLTNLMHYLIGFAIAVVSAFVATWLLKFRE 455 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,549,249,988 Number of extensions: 97421393 Number of successful extensions: 220354 Number of sequences better than 1.0e-05: 1693 Number of HSP's gapped: 214714 Number of HSP's successfully gapped: 1708 Length of query: 496 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 356 Effective length of database: 1,741,754,103 Effective search space: 620064460668 Effective search space used: 620064460668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)