BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0075.p (448 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4ZG45.1 C4ZG45_9FIRM Glycosyltransferase Family 2 modular protein 139 8e-31 A8U5L7.1 A8U5L7_9LACT General stress protein A 107 2e-21 Q38ZB8.1 Q38ZB8_LACSS Putative Bifunctional glycosyl transferase... 99 1e-18 C2L3X8.1 C2L3X8_LACSK Bifunctional glycosyl transferase family p... 99 1e-18 Q65DI7.1 Q65DI7_BACLD GspA 97 4e-18 >C4ZG45.1 C4ZG45_9FIRM Glycosyltransferase Family 2 modular protein Length = 723 Score = 139 bits (349), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 88/265 (33%), Positives = 147/265 (55%), Gaps = 15/265 (5%) Query: 2 VELVLTINDKDGSYTEHAAVVLASIFSKTEQTINVHIVHDDSLSEENKVKLTQMVDVFHH 61 + + L I+DKDG+Y+ A + SI T+ I HI+HDD+L+E NK KL+ + D + Sbjct: 343 IHICLGIHDKDGNYSVWAGTTMQSIVENTKAPIVFHILHDDTLNEMNKNKLSLIADNSGN 402 Query: 62 NIFFYHVTIPGDMREVAAGVHKIDYWTMASMYRLLLPIII-QTDRVIYLDCDILVNMDIN 120 I F+H P +A +++ +T+ +M+R++LP I+ ++IYLD D+ VN DI Sbjct: 403 GIEFHHFN-PDIFGSLADSMNR---FTIGTMFRIMLPDIMPDLKKIIYLDSDLFVNTDIE 458 Query: 121 ELWSIDLGDRYLGAVLDQGENLLEYFTSMGLSA-----ELYFNSGVILFHLDNIRKKDNW 175 ELW++++ D Y A + + T ++A + YFN+GV+ +LDNIRK + Sbjct: 459 ELWNLNI-DNYCLAAAQDCSTIRNWGTPYAVAAGQTSRDRYFNAGVLCMNLDNIRKNGSL 517 Query: 176 YGEMLNFLRNFPKMTMPDQDVLNAVYSCNYLQLDQCFNTF----AQNELDLEHKIIHFAG 231 + +++++L + P+ +PDQD LNA++S L +D+ +N F +N E KI H+A Sbjct: 518 FQQVMDYLSDNPRTWLPDQDALNAIFSGKTLLIDEKWNYFIDEARKNNEKAEKKIYHYAA 577 Query: 232 ENKWWDADSPHVTLYNNFRAMTPWG 256 ++ Y TPWG Sbjct: 578 TLLMLHTNNEIDRAYYFTILRTPWG 602 >A8U5L7.1 A8U5L7_9LACT General stress protein A Length = 284 Score = 107 bits (268), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 87/260 (33%), Positives = 134/260 (51%), Gaps = 36/260 (13%) Query: 8 INDKDGSYTEHAAVVLASIFSKTEQTINVHI-VHDDSLSEENKVKLTQMVDVFHHNIFFY 66 ++ + ++ H A + S+ + + H V DD++S +K L + V + I + Sbjct: 10 VSSSNEAFVPHLATLFRSLLTTKQTHTTFHFYVIDDNISLHSKFLLNRTVGEYDARISY- 68 Query: 67 HVTIPGDMREVAAGVHKIDYWTMASMYRLLLP-IIIQTDRVIYLDCDILVNMDINELWSI 125 VTI D E A V D + YR+ +P ++ +T R IY+DCD++ DI LW + Sbjct: 69 -VTI--DPTEFAGAVES-DRIPQTAYYRISIPNLLKETKRAIYMDCDMISLEDIEGLWEV 124 Query: 126 DLGDRYLGAVLDQG-ENLLEYFTSMGLSAE--LYFNSGVILFHLDNIRKKDNWYGEMLNF 182 DLGD+ LGAV D G N LE MG+ +E LYFNSG+++ L+ R ++ ++L F Sbjct: 125 DLGDKLLGAVEDAGFHNRLE---KMGIESETDLYFNSGLMVMDLEKWR-EEKITEQVLAF 180 Query: 183 LRNFP-KMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNEL---DLEH-------------- 224 + N P K+ DQD LNA+ +L+LD +N AQ + ++EH Sbjct: 181 IENNPEKLRFHDQDALNAILHDRWLELDPRWN--AQTYMMLQEVEHPTIQGQLKWDEARE 238 Query: 225 --KIIHFAGENKWWDADSPH 242 +IHF G K W+ADS H Sbjct: 239 NPAVIHFCGHAKPWNADSNH 258 >Q38ZB8.1 Q38ZB8_LACSS Putative Bifunctional glycosyl transferase, family 8 Length = 566 Score = 98.6 bits (244), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 76/251 (30%), Positives = 129/251 (51%), Gaps = 27/251 (10%) Query: 12 DGSYTEHAAVVLASIFSK--TEQTINVHIVHDDSLSEENKVKLTQMVDVFHHNIFFYHVT 69 + ++ E A++ ASI + T++ + +++ +D L+E +K+ L Q+V F+ ++ F V Sbjct: 288 NAAFIEPLAILYASILNHNDTQRRYSFYVL-EDHLTEADKIPLKQVVAAFNADLTFLKVD 346 Query: 70 IPGDMREVAAGVHKIDYWTMASMYRLLLPIIIQ-TDRVIYLDCDILVNMDINELWSIDLG 128 + A + + D ++ YR+L+P ++ DR +YLDCD L N+++ LW+IDLG Sbjct: 347 -----EALLANIVESDRILKSAYYRILIPQVLNGIDRALYLDCDALCNVNLERLWNIDLG 401 Query: 129 DRYLGAVLDQGENLLEYFTSMGLSAELYFNSGVILFHLDNIRKKDNWYGEMLNFLRNFP- 187 + L AV D G + ++ + YFNSG++L L R++ + L+F+ + P Sbjct: 402 EFPLAAVEDAGFHQRLEKMAIKCHSTRYFNSGMMLMDLKKWRQQA-ITEKTLDFINHHPE 460 Query: 188 KMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELD------------LEHK----IIHFAG 231 K+ DQD LNAV +L L +N +D +E K I+HF G Sbjct: 461 KLRFHDQDALNAVLHDQWLHLHPKWNAQTNIIMDKTTPPQHLQQQFIEAKKAPAIVHFCG 520 Query: 232 ENKWWDADSPH 242 K W A S H Sbjct: 521 HEKPWHAVSTH 531 Score = 83.2 bits (204), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 74/269 (27%), Positives = 125/269 (46%), Gaps = 40/269 (14%) Query: 4 LVLTIN---DKDGSYTEHAAVVLASIFSKTEQTINVH-IVHDDSLSEENKVKLTQMVDVF 59 ++ TIN D +Y + + + SI + VH ++ D+ L+ + K + Q+ Sbjct: 2 IIKTINVMFAADNNYADQLLIAIKSILAHIPAETTVHFLILDNELTPQTKCLVRQITKQ- 60 Query: 60 HHNIFFYHVTIPGDMREVAAGVHKIDYWTMASMYRLLLPIII---QTDRVIYLDCDILVN 116 H + F + +++ + ++ + YR+L P I+ RV+YLD DILV Sbjct: 61 SHQVDFIKIN-----QQLLKNCPESNHINKTAYYRILAPQILLRKGISRVLYLDVDILVQ 115 Query: 117 MDINELWSIDLGDRYLGAVLDQGENLLEYFTSMGLSAE----LYFNSGVILFHLDNIRKK 172 DI L+ LG +GA++D G+ L +G+S E +YFNSGV+L +D R + Sbjct: 116 TDITPLYESHLGTNIVGAIIDPGQALA--LPRLGVSPEKSGNIYFNSGVML--IDTFRWE 171 Query: 173 DNWYGEM-LNFLRNFP-KMTMPDQDVLNAVYSCNYLQLDQCFN---------------TF 215 +N E+ L F+ P ++ DQD LNA+ + L +N T+ Sbjct: 172 ENQISELTLRFINQHPERIIFHDQDALNAILAGKVQLLHPAWNVQNSLIFRKHQPINATY 231 Query: 216 AQ--NELDLEHKIIHFAGENKWWDADSPH 242 + +E + KI+HF NK W+ H Sbjct: 232 KKLFDEAIAQPKIVHFTTHNKPWNTLKGH 260 >C2L3X8.1 C2L3X8_LACSK Bifunctional glycosyl transferase family protein Length = 566 Score = 98.6 bits (244), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 76/251 (30%), Positives = 129/251 (51%), Gaps = 27/251 (10%) Query: 12 DGSYTEHAAVVLASIFSK--TEQTINVHIVHDDSLSEENKVKLTQMVDVFHHNIFFYHVT 69 + ++ E A++ ASI + T++ + +++ +D L+E +K+ L Q+V F+ ++ F V Sbjct: 288 NAAFIEPLAILYASILNHNDTQRRYSFYVL-EDHLTEADKIPLKQVVAAFNADLTFLKVD 346 Query: 70 IPGDMREVAAGVHKIDYWTMASMYRLLLPIIIQ-TDRVIYLDCDILVNMDINELWSIDLG 128 + A + + D ++ YR+L+P ++ DR +YLDCD L N+++ LW+IDLG Sbjct: 347 -----EALLANIVESDRILKSAYYRILIPQVLNGIDRALYLDCDALCNVNLERLWNIDLG 401 Query: 129 DRYLGAVLDQGENLLEYFTSMGLSAELYFNSGVILFHLDNIRKKDNWYGEMLNFLRNFP- 187 + L AV D G + ++ + YFNSG++L L R++ + L+F+ + P Sbjct: 402 EFPLAAVEDAGFHQRLEKMAIKCHSTRYFNSGMMLMDLKKWRQQA-ITEKTLDFINHHPE 460 Query: 188 KMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELD------------LEHK----IIHFAG 231 K+ DQD LNAV +L L +N +D +E K I+HF G Sbjct: 461 KLRFHDQDALNAVLHDQWLHLHPKWNAQTNIIMDKTTPPQHLQQQFIEAKKAPAIVHFCG 520 Query: 232 ENKWWDADSPH 242 K W A S H Sbjct: 521 HEKPWHAVSTH 531 Score = 82.8 bits (203), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 71/258 (27%), Positives = 120/258 (46%), Gaps = 37/258 (14%) Query: 12 DGSYTEHAAVVLASIFSKTEQTINVH-IVHDDSLSEENKVKLTQMVDVFHHNIFFYHVTI 70 D +Y + + + SI + VH ++ D+ L+ + K + Q+ H + F + Sbjct: 13 DNNYADQLLIAIKSILAHIPAETTVHFLILDNELTPQTKCLVRQITKQ-SHQVDFIKIN- 70 Query: 71 PGDMREVAAGVHKIDYWTMASMYRLLLPIII---QTDRVIYLDCDILVNMDINELWSIDL 127 +++ + ++ + YR+L P I+ RV+YLD DILV DI L+ L Sbjct: 71 ----QQLLKNCPESNHINKTAYYRILAPQILLRKGISRVLYLDVDILVQTDITPLYESHL 126 Query: 128 GDRYLGAVLDQGENLLEYFTSMGLSAE----LYFNSGVILFHLDNIRKKDNWYGEM-LNF 182 G +GA++D G+ L +G+S E +YFNSGV+L +D R ++N E+ L F Sbjct: 127 GTNIVGAIIDPGQALA--LPRLGVSPEKSGNIYFNSGVML--IDTFRWEENQISELTLRF 182 Query: 183 LRNFP-KMTMPDQDVLNAVYSCNYLQLDQCFN---------------TFAQ--NELDLEH 224 + P ++ DQD LNA+ + L +N T+ + +E + Sbjct: 183 INQHPERIIFHDQDALNAILAGKVQLLHPAWNVQNSLIFRKHQPINATYKKLFDEAIAQP 242 Query: 225 KIIHFAGENKWWDADSPH 242 KI+HF NK W+ H Sbjct: 243 KIVHFTTHNKPWNTLKEH 260 >Q65DI7.1 Q65DI7_BACLD GspA Length = 287 Score = 97.1 bits (240), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 132/261 (50%), Gaps = 36/261 (13%) Query: 8 INDKDGSYTEHAAVVLASIFSKTEQTINVHI-VHDDSLSEENKVKLTQMVDVFHHNIFFY 66 I+ D +Y +H +V+ +S+ + +QT V + V D + ENK +L + F I F Sbjct: 10 ISCTDNNYAQHLSVMFSSLLTNMDQTREVKLYVIDGGIEPENKKRLEETTLQFGAPITFL 69 Query: 67 HVTIPGDMREVAAGVHKIDYWTMASMYRLLLPIIIQTD---RVIYLDCDILVNMDINELW 123 +V + V + + T A+ YR+ +P +I + R+IY+DCD LV DI++LW Sbjct: 70 NVEKSQYDKAVESS-----HITKAAYYRISIPDLIDDESVKRMIYVDCDALVLEDISKLW 124 Query: 124 SIDLGDRYLGAVLDQGENLLEYFTSMGLSAEL-YFNSGVILFHLDNIRKKDNWYGEMLNF 182 +D+ + AV D G++ E M +S E YFNSG+++ ++ RK D ++++F Sbjct: 125 DMDISPYFAAAVEDAGQH--ERLKKMNISDEAKYFNSGLMIIDMEAWRKND-ISKKVIDF 181 Query: 183 LRNFPK--MTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELDLEHK--------------- 225 + N + DQD LNA+ +L+L +N AQ + L+ K Sbjct: 182 INNNDEDMFVFHDQDALNAILYDQWLELHPRWN--AQTHIILKEKTPASLIDQKRYMETR 239 Query: 226 ----IIHFAGENKWWDADSPH 242 I+HF G NK W++++ H Sbjct: 240 ANPAIVHFCGGNKPWNSNTSH 260 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,123,298,060 Number of extensions: 82174551 Number of successful extensions: 218723 Number of sequences better than 1.0e-05: 442 Number of HSP's gapped: 218431 Number of HSP's successfully gapped: 448 Length of query: 448 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 309 Effective length of database: 1,751,175,118 Effective search space: 541113111462 Effective search space used: 541113111462 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)