BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0069.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8F9G1.1 A8F9G1_BACP2 Possible alpha/beta family hydrolase 395 e-108 B4AMV7.1 B4AMV7_BACPU Putative alpha/beta family hydrolase 389 e-106 B3K727.1 B3K727_9BACI Putative alpha/beta family hydrolase 375 e-102 C3FVW1.1 C3FVW1_BACTB Alpha/beta family hydrolase 365 4e-99 C3DCD9.1 C3DCD9_BACTU Alpha/beta family hydrolase 365 4e-99 >A8F9G1.1 A8F9G1_BACP2 Possible alpha/beta family hydrolase Length = 315 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/312 (58%), Positives = 244/312 (78%), Gaps = 1/312 (0%) Query: 12 MRKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTIQ 71 M+KI +L +I ++ + +FL +VY VN+ S++ E KI+ YGQ V VDGK MNV IQ Sbjct: 1 MKKILRIMLISIGTIITVMVVFLASVYFVNLFSNEREKGKIEAYGQKVMVDGKQMNVLIQ 60 Query: 72 GKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIVA 131 G G+ET+VLLPGYGTA+P LD++PLVKELSP+YRVVVIEPFGYGLS+ +DK RT +NIV Sbjct: 61 GDGKETIVLLPGYGTASPVLDFKPLVKELSPYYRVVVIEPFGYGLSDDTDKVRTSQNIVN 120 Query: 132 EIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGLEELPAGA 191 EIHECLQ+LNI++YT+MGHSI+GIYGL+YVNQY+ EV AFVGIDSSFP Q +ELP + Sbjct: 121 EIHECLQKLNINKYTVMGHSISGIYGLEYVNQYEKEVQAFVGIDSSFPKQEDADELPVSS 180 Query: 192 YKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISESENFKHNFQA 251 +LL SGFYRL+VK+NPDQ++ P V +TKAQI+ML+ +N M+ + SE++NF++NF+ Sbjct: 181 LQLLHQSGFYRLIVKMNPDQLVMPKVGDQTKAQIKMLTFRNFMDESQASEADNFRNNFKN 240 Query: 252 AESFSFPADLPVIFFLADDN-MDVPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKEI 310 A+ FP LPV+F+LAD + P+W P H+E +K+ HGKV+T +G HYLHHT+SKEI Sbjct: 241 AQRLHFPKKLPVVFYLADQTEKETPNWKPMHEELLKNVDHGKVVTFKGGHYLHHTKSKEI 300 Query: 311 VQNFRSFMAGIQ 322 +FR F++ I+ Sbjct: 301 AADFREFLSEIE 312 >B4AMV7.1 B4AMV7_BACPU Putative alpha/beta family hydrolase Length = 315 Score = 389 bits (1000), Expect = e-106, Method: Compositional matrix adjust. Identities = 182/312 (58%), Positives = 240/312 (76%), Gaps = 1/312 (0%) Query: 12 MRKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTIQ 71 M+KI +L +I ++ + +FL TVY VN+IS++ E KI+ YGQ V VDGK MNV IQ Sbjct: 1 MKKILRIMLISIGTIITVMVVFLATVYFVNLISNEREKGKIEAYGQKVLVDGKQMNVLIQ 60 Query: 72 GKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIVA 131 G G+ET+VLLPGYGTA+P LD++PLV ELSP+YRVVVIEPFGYGLS+ +DK RT +NIV Sbjct: 61 GDGKETIVLLPGYGTASPVLDFKPLVDELSPYYRVVVIEPFGYGLSDDTDKVRTSQNIVD 120 Query: 132 EIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGLEELPAGA 191 EIHECLQ+LNI +YTLMGHSI+GIYGL+YVNQY+ EV AFVGIDSS P Q +ELP + Sbjct: 121 EIHECLQKLNIKKYTLMGHSISGIYGLEYVNQYEKEVQAFVGIDSSVPKQEDADELPVSS 180 Query: 192 YKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISESENFKHNFQA 251 +LL SGFYRL+VK+NPDQ++ P VD +TK QI+ML+ +N +N + SE+ENF++NF+ Sbjct: 181 LQLLHQSGFYRLIVKMNPDQLLMPKVDDQTKDQIKMLTFQNFLNESQASEAENFRNNFKN 240 Query: 252 AESFSFPADLPVIFFLADDN-MDVPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKEI 310 A+ P +LPV+F+LAD + P W P H++ +K+ GKV+T +G HYLHHT+SKEI Sbjct: 241 AQRLHLPKNLPVVFYLADQTEEETPSWKPMHEDLLKNVDRGKVVTFKGGHYLHHTKSKEI 300 Query: 311 VQNFRSFMAGIQ 322 +FR F++ I+ Sbjct: 301 AADFREFLSNIE 312 >B3K727.1 B3K727_9BACI Putative alpha/beta family hydrolase Length = 331 Score = 375 bits (964), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/311 (59%), Positives = 232/311 (74%), Gaps = 1/311 (0%) Query: 13 RKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTIQG 72 +K S LLK I ++ I LFLG VYI NVISS SEA +I+ YGQ VSVDGKNMNV IQG Sbjct: 16 KKARSILLKIIGAIVIAIILFLGIVYISNVISSHSEAKRIEPYGQQVSVDGKNMNVYIQG 75 Query: 73 KGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIVAE 132 +GEET+VLLPG GTAAP LD++ L+ ELSPFYRVV +EPFGYGLS+ +DK RT ENIV+E Sbjct: 76 EGEETIVLLPGAGTAAPALDFKLLIDELSPFYRVVAVEPFGYGLSDGTDKERTTENIVSE 135 Query: 133 IHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGLE-ELPAGA 191 +HE LQQLNI RYTLMGHSIAGIYG+DYV++Y +EVTAFVGIDSS PTQ G++ + P Sbjct: 136 VHEALQQLNITRYTLMGHSIAGIYGIDYVSKYPNEVTAFVGIDSSVPTQPGMDVKFPIKK 195 Query: 192 YKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISESENFKHNFQA 251 + LK SG RL++KL PD A A D TK Q+R+++ KN + I +E E+ NF+ Sbjct: 196 FAFLKQSGLMRLMMKLGPDPYAALAFDDHTKEQMRLITNKNALTATIFNEMEHIPSNFKG 255 Query: 252 AESFSFPADLPVIFFLADDNMDVPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKEIV 311 A+ +FP DLP++ F+ DN D P W+P H+EQ+KDS +GKV+ LEGEHYLHHT+ K I Sbjct: 256 AQDLTFPKDLPLLLFVQADNPDFPTWIPLHEEQVKDSDYGKVIPLEGEHYLHHTKYKNIA 315 Query: 312 QNFRSFMAGIQ 322 + F F+ ++ Sbjct: 316 ETFSEFIEEVK 326 >C3FVW1.1 C3FVW1_BACTB Alpha/beta family hydrolase Length = 328 Score = 365 bits (936), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 177/313 (56%), Positives = 230/313 (73%), Gaps = 1/313 (0%) Query: 11 QMRKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTI 70 +++K+ S LLK I ++ + LFLG VYIVNV+SS SE +I YGQ VSVDGKNMNV I Sbjct: 16 KIKKVRSILLKTIGAIVLAIVLFLGIVYIVNVMSSHSEQKRIVPYGQHVSVDGKNMNVLI 75 Query: 71 QGKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIV 130 QG+GEET+VLLPGYGTA PGLD++ L+ ELSPFY+VV +EPFGYGLS+ + K RT EN+V Sbjct: 76 QGEGEETIVLLPGYGTATPGLDFKLLIDELSPFYKVVAVEPFGYGLSDETVKERTTENMV 135 Query: 131 AEIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGLE-ELPA 189 +E+HE LQQLNI+RY LMGHSI GIYG+DYVN+Y +EVTAFVGIDSS TQ G++ P Sbjct: 136 SEVHEALQQLNINRYMLMGHSITGIYGIDYVNKYPNEVTAFVGIDSSVATQPGMDINFPL 195 Query: 190 GAYKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISESENFKHNF 249 + LK SG RL +K++ D A D +T Q++M+S KNM N ++E ++ NF Sbjct: 196 KTFAYLKKSGLLRLAMKISADPYAGLAFDEKTVEQMKMISNKNMYNDTTLNEMDHISSNF 255 Query: 250 QAAESFSFPADLPVIFFLADDNMDVPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKE 309 + A+ FP LP++ FL +N DV W+P H+ QIKDSVHGKV+T++G HYLHHT+ KE Sbjct: 256 KGAQGLIFPKYLPLLLFLQANNEDVAGWIPLHEGQIKDSVHGKVVTMDGSHYLHHTKFKE 315 Query: 310 IVQNFRSFMAGIQ 322 I +N R FM ++ Sbjct: 316 IAENVRLFMKEVK 328 >C3DCD9.1 C3DCD9_BACTU Alpha/beta family hydrolase Length = 328 Score = 365 bits (936), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 177/313 (56%), Positives = 230/313 (73%), Gaps = 1/313 (0%) Query: 11 QMRKIFSFLLKAIISLFILTGLFLGTVYIVNVISSKSEAAKIKTYGQLVSVDGKNMNVTI 70 +++K+ S LLK I ++ + LFLG VYIVNV+SS SE +I YGQ VSVDGKNMNV I Sbjct: 16 KIKKVRSILLKTIGAIVLAIVLFLGIVYIVNVMSSHSEQKRIVPYGQHVSVDGKNMNVLI 75 Query: 71 QGKGEETVVLLPGYGTAAPGLDYQPLVKELSPFYRVVVIEPFGYGLSEVSDKARTIENIV 130 QG+GEET+VLLPGYGTA PGLD++ L+ ELSPFY+VV +EPFGYGLS+ + K RT EN+V Sbjct: 76 QGEGEETIVLLPGYGTATPGLDFKLLIDELSPFYKVVAVEPFGYGLSDETVKERTTENMV 135 Query: 131 AEIHECLQQLNIHRYTLMGHSIAGIYGLDYVNQYKDEVTAFVGIDSSFPTQGGLE-ELPA 189 +E+HE LQQLNI+RY LMGHSI GIYG+DYVN+Y +EVTAFVGIDSS TQ G++ P Sbjct: 136 SEVHEALQQLNINRYMLMGHSITGIYGIDYVNKYPNEVTAFVGIDSSVATQPGMDINFPL 195 Query: 190 GAYKLLKNSGFYRLLVKLNPDQIIAPAVDAETKAQIRMLSLKNMMNPNIISESENFKHNF 249 + LK SG RL +K++ D A D +T Q++M+S KNM N ++E ++ NF Sbjct: 196 KTFAYLKKSGLLRLAMKISADPYAGLAFDEKTVEQMKMISNKNMYNDTTLNEMDHISSNF 255 Query: 250 QAAESFSFPADLPVIFFLADDNMDVPDWVPKHKEQIKDSVHGKVMTLEGEHYLHHTRSKE 309 + A+ FP LP++ FL +N DV W+P H+ QIKDSVHGKV+T++G HYLHHT+ KE Sbjct: 256 KGAQGLIFPKYLPLLLFLQANNEDVAGWIPLHEGQIKDSVHGKVVTMDGSHYLHHTKFKE 315 Query: 310 IVQNFRSFMAGIQ 322 I +N R FM ++ Sbjct: 316 IAENVRLFMKEVK 328 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,882,720,020 Number of extensions: 78119493 Number of successful extensions: 251800 Number of sequences better than 1.0e-05: 242 Number of HSP's gapped: 252223 Number of HSP's successfully gapped: 242 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)