BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0050.p (232 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8L9.1 B3K8L9_9BACI Nicotinamide mononucleotide transporter PnuC 337 4e-91 B8FBX0.1 B8FBX0_DESAA Nicotinamide mononucleotide transporter PnuC 185 4e-45 Q2B419.1 Q2B419_9BACI PnuC protein 158 3e-37 A7B3K5.1 A7B3K5_RUMGN Putative uncharacterized protein 145 2e-33 C4C232.1 C4C232_9FUSO Nicotinamide mononucleotide transporter PnuC 143 1e-32 >B3K8L9.1 B3K8L9_9BACI Nicotinamide mononucleotide transporter PnuC Length = 225 Score = 337 bits (865), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 175/219 (79%), Positives = 192/219 (87%) Query: 14 GWNLFELSWLVLFTAIAVGFTVISKDSXXXXXXXXXXXXXXXXAAKGNLMNYVFGMYNTV 73 GWNLFE++WL LFT+IAVGFTV+SKDS AAKGNLM+YVFGMYNT+ Sbjct: 7 GWNLFEIAWLALFTSIAVGFTVMSKDSLFGFTVFITGVLCVVLAAKGNLMSYVFGMYNTI 66 Query: 74 GYAYLAYVNGLFGEVMLNLLFFVPMNVVGFYMWKNNSNHGKLSMRQLELKGLLLVGAVCI 133 GYAYLAY+NGLFGEVMLNLLFFVPMNV+GFYMWKN+ GKL MRQ+EL+GLLLVGAVC+ Sbjct: 67 GYAYLAYINGLFGEVMLNLLFFVPMNVIGFYMWKNHRQDGKLIMRQMELRGLLLVGAVCV 126 Query: 134 AGCLLLGFGLSFIPGQNSPYIDAITTVLSVAATLLMVRRFKEQWLVYIVLNMFTVLLWVI 193 GCLLLG+GLSFIPGQNSPYIDA TTVLSV AT LMVRRFKEQWLVYIVLN+FTVLLW I Sbjct: 127 LGCLLLGYGLSFIPGQNSPYIDATTTVLSVVATFLMVRRFKEQWLVYIVLNLFTVLLWAI 186 Query: 194 RMLEGSGEGLLMIVMWSAYLVNAVYGYYNWNKGAREVAA 232 R+LEGS EG+LMIVMWSAYLVNAVYGYYNWNKGA+EVAA Sbjct: 187 RLLEGSSEGMLMIVMWSAYLVNAVYGYYNWNKGAKEVAA 225 >B8FBX0.1 B8FBX0_DESAA Nicotinamide mononucleotide transporter PnuC Length = 230 Score = 185 bits (469), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 98/217 (45%), Positives = 136/217 (62%) Query: 16 NLFELSWLVLFTAIAVGFTVISKDSXXXXXXXXXXXXXXXXAAKGNLMNYVFGMYNTVGY 75 ++ ++ W+ L AV F++ DS A G + Y GMY + Y Sbjct: 6 DVKDVVWVALLLIAAVCFSIAMGDSLFASSVLISGILCVALTAIGRIEGYAAGMYASCTY 65 Query: 76 AYLAYVNGLFGEVMLNLLFFVPMNVVGFYMWKNNSNHGKLSMRQLELKGLLLVGAVCIAG 135 A++AY NGLFGEVMLNL FF+P N++GF MW+ + MRQL + +VGAV I G Sbjct: 66 AWMAYQNGLFGEVMLNLGFFIPTNIIGFMMWRKKMRDNVVEMRQLIWRHRAMVGAVVILG 125 Query: 136 CLLLGFGLSFIPGQNSPYIDAITTVLSVAATLLMVRRFKEQWLVYIVLNMFTVLLWVIRM 195 L LG LS IPGQN+PYIDA T V+S+ ATLLM+ R+KEQW++YI+LN + +W++R Sbjct: 126 TLGLGLILSRIPGQNTPYIDASTNVISIIATLLMMWRYKEQWMLYIILNTVSTAMWILRW 185 Query: 196 LEGSGEGLLMIVMWSAYLVNAVYGYYNWNKGAREVAA 232 G G LMIVMW YL NA++GY+ W+ GA++++A Sbjct: 186 RAGGASGDLMIVMWMIYLANAIFGYWRWHTGAKKLSA 222 >Q2B419.1 Q2B419_9BACI PnuC protein Length = 231 Score = 158 bits (400), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 85/228 (37%), Positives = 131/228 (57%), Gaps = 6/228 (2%) Query: 10 KSWGGWNLFELSWLVLFTAIAVGFTVISKDSXXXXXXXXXXXXXXXXAAKGNLMNYVFGM 69 K + W +FE+SWL++F I V + +DS AKG + NY FG+ Sbjct: 2 KIFRDWTIFEISWLLVFILINVYLFIAWQDSLLGLISSLAGMLCVVLVAKGKIANYYFGI 61 Query: 70 YNTVGYAYLAYVNGLFGEVMLNLLFFVPMNVVGFYMWKNNSNHGKLS-----MRQLELKG 124 T YAY++Y GL+GE MLN LF+ P+ +G Y+W+ N + ++ L+ KG Sbjct: 62 IQTGTYAYISYTYGLYGEAMLNGLFYFPIQFIGIYLWRKNMTKDSIKGEDVKVKTLDSKG 121 Query: 125 LLLVGAVCIAGCLLLGFGLSFIPGQNSPYIDAITTVLSVAATLLMVRRFKEQWLVYIVLN 184 L+G + L L ++ G+ S YID+ T VLSV A +LM+RRF EQWL++I++N Sbjct: 122 WSLLGITVLVSIFLYALLLDYLGGR-SVYIDSATNVLSVTAQILMLRRFAEQWLLWILVN 180 Query: 185 MFTVLLWVIRMLEGSGEGLLMIVMWSAYLVNAVYGYYNWNKGAREVAA 232 + +V+LW ++ G + M+VMW+A+L N++YGY NW K ++ A Sbjct: 181 VLSVILWFNALISTGGNDISMLVMWTAFLFNSIYGYINWTKLYKKQGA 228 >A7B3K5.1 A7B3K5_RUMGN Putative uncharacterized protein Length = 230 Score = 145 bits (367), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 80/225 (35%), Positives = 131/225 (58%), Gaps = 4/225 (1%) Query: 7 QVNKSWGGWNLFELSWLVLFTAIAVGFTVISKDSXXXXXXXXXXXXXXXXAAKGNLMNYV 66 +V + GW+ FEL WLVL T I + ++I D+ AAKG + Y Sbjct: 3 KVKDFFKGWSKFELCWLVLSTVIMIVLSIIWGDNTLALISGITGILGVVLAAKGKVSTYF 62 Query: 67 FGMYNTVGYAYLAYVNGLFGEVMLNLLFFVPMNVVGFYMWKNNSNH--GKLSMRQLELKG 124 F N YAYL + N L+GE MLN +++PMN +GFY+W + + G++ + L K Sbjct: 63 FATINVAIYAYLTFNNHLYGEFMLNAFYYIPMNFIGFYLWSRHKDEKSGEVEGKALTPKQ 122 Query: 125 LLLVGAVCIAGCLLLGFGLSFIPGQNSPYIDAITTVLSVAATLLMVRRFKEQWLVYIVLN 184 ++++ A ++ L+ + GQ + IDA++TV SV A ++ V R+ EQWL++I++N Sbjct: 123 IVILLAATAVIVIIYWQILTHLGGQLA-LIDAMSTVFSVIALIMQVARYAEQWLLWIIVN 181 Query: 185 MFTVLLWVIRMLEGSGEGLLMIVMWSAYLVNAVYGYYNWNKGARE 229 + +V++W++ ++ + M+VMW AYL N++YGYYNW K A + Sbjct: 182 IVSVIMWIL-LIGKDSSAVTMVVMWIAYLFNSIYGYYNWKKLAAK 225 >C4C232.1 C4C232_9FUSO Nicotinamide mononucleotide transporter PnuC Length = 230 Score = 143 bits (361), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 80/225 (35%), Positives = 128/225 (56%), Gaps = 12/225 (5%) Query: 10 KSWGGWNLFELSWLVLFTAIAVGFTVISKDSXXXXXXXXXXXXXXXXAAKGNLMNYVFGM 69 K + WNLFE SWL++FT I V + SK+ AKG + NY FG+ Sbjct: 2 KIFKDWNLFEKSWLIIFTLINVAVLIYSKEGILGFTASVTGMLSVILVAKGKISNYYFGI 61 Query: 70 YNTVGYAYLAYVNGLFGEVMLNLLFFVPMNVVGFYMWKNN----SNHGKLSMRQLELKGL 125 N V Y +++Y + +GE MLN+L+F+PM ++GF MW+ N ++ ++ K + Sbjct: 62 INVVIYGFISYNSKYYGEAMLNILYFLPMQIIGFMMWRRNNVNIDESKEVKAERMTAKEI 121 Query: 126 LLVGAVCIAGCLLLGFGLSFIPGQNS-PYIDAITTVLSVAATLLMVRRFKEQWLVYIVLN 184 +L ++G ++ +G+ N+ P D+ TTVLSV A +LMV+R+ EQW+V+I+++ Sbjct: 122 ILWS--VLSGIAVIVYGIILKKLNNTLPMADSFTTVLSVTAMILMVKRYIEQWIVWIMID 179 Query: 185 MFTVLLWVIRMLEGSGEGLLMIVMWSAYLVNAVYGYYNWNKGARE 229 + + +W+ + + + +MW AYLVNAVYG YNW K R Sbjct: 180 IVAIYMWLFIKSDYN-----ITIMWIAYLVNAVYGLYNWAKLYRR 219 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.141 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,304,785,394 Number of extensions: 46858714 Number of successful extensions: 184723 Number of sequences better than 1.0e-05: 501 Number of HSP's gapped: 184012 Number of HSP's successfully gapped: 501 Length of query: 232 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 101 Effective length of database: 1,826,543,238 Effective search space: 184480867038 Effective search space used: 184480867038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)