BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0025.p (389 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q58GF0.1 Q58GF0_BACSU Subtilisin AP01 282 4e-74 B8YDC8.1 B8YDC8_BACSU Fibrinolytic enzyme F1 281 9e-74 B4ALD7.1 B4ALD7_BACPU Subtilisin Carlsberg EC=3.4.21.62 280 2e-73 B6VFQ8.1 B6VFQ8_BACSU Subtilisin (Fragment) 278 9e-73 A0FLP3.1 A0FLP3_BACSU Subtilisin 277 1e-72 >Q58GF0.1 Q58GF0_BACSU Subtilisin AP01 Length = 381 Score = 282 bits (721), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 171/384 (44%), Positives = 235/384 (61%), Gaps = 17/384 (4%) Query: 4 IRLLSSFCLI-TVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGG 61 I LL +F LI T+ ++ A AA ++S+ K Y + + +++T DVI + GG Sbjct: 8 ISLLFAFTLIFTMAFSNMSAQAAGKNSEE---KKYIVGFKQTMGAMSTAKKKDVISEKGG 64 Query: 62 KLKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQ 121 K++K + + A L ++ ELK P V Y+E ++ V E +SV PYGI Sbjct: 65 KVQKQFKYVNAAAATLDDKAVKELKKDPSVAYVEEDH-----VAHEYAQSV----PYGIS 115 Query: 122 NIHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVA 179 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVA Sbjct: 116 QIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVA 175 Query: 180 GIIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGE 239 G +AA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG Sbjct: 176 GTVAALNNTIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGP 235 Query: 240 NPSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFF 298 S L+ + +A +GI+++AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA F Sbjct: 236 TGSTALKTVVDKAVASGIVVVAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASF 295 Query: 299 SAKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADI 358 S+ G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ S +V D Sbjct: 296 SSAGSELDVMAPGVSIQSTLPGGTYGSYNGTSMATPHVAGAAALILSKHPTWSNAQVRDR 355 Query: 359 LESTAEPLGVVPEYGHGIVQAKEA 382 LESTA LG YG G++ + A Sbjct: 356 LESTATNLGSSFYYGKGLINVQAA 379 >B8YDC8.1 B8YDC8_BACSU Fibrinolytic enzyme F1 Length = 381 Score = 281 bits (718), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 166/383 (43%), Positives = 229/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L+ ++ ELK P V Y+E E +++PYGI Sbjct: 66 IQKQFKYVNAATATLNEKAVKELKQDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG + Sbjct: 177 TIAALNNSIGVLGVAPNASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPS 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSNGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + GP+LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SAGPELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGNSFYYGKGLINVQAA 379 >B4ALD7.1 B4ALD7_BACPU Subtilisin Carlsberg EC=3.4.21.62 Length = 376 Score = 280 bits (715), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 166/387 (42%), Positives = 238/387 (61%), Gaps = 18/387 (4%) Query: 1 MKIIRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTT--PPNDVIEK 58 M + + + C+ ++ML S AS S AK Y++ +S ++E+ Sbjct: 1 MNVKSIGAGLCMASMMLASV-TFGASHISAKEQAKK---EYMIGFSSSVQDHTQKQLVEE 56 Query: 59 LGGKLKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPY 118 GG +K+ E D++ L+ +S +L + +V +IE + + KT +TIPY Sbjct: 57 AGGHVKESIEQIDMMKVSLNEASKKKLNQAKEVTFIEEDQKAKTS---------GQTIPY 107 Query: 119 GIQNIHAIEAQQEGYTGKGVKIAVLDSGYS-QHSDVKVKSGYSFI-TNSENYDDDFGHGT 176 GI++I A + + GY G+ VK+AVLDSG +H D+ V G SF+ T S+ D HGT Sbjct: 108 GIKSIKAQKVHKRGYAGQNVKVAVLDSGIDGKHEDLHVTGGVSFVPTESDPLVDPHEHGT 167 Query: 177 HVAGIIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISF 236 HVAG IAA N GVVGVAP A IYA+KV D+ G G Y +I+GI+WAI++ +D+INIS Sbjct: 168 HVAGTIAALDNKVGVVGVAPKASIYAVKVADENGDGYYSWIIKGIEWAIENDIDVINISM 227 Query: 237 GGENPSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKR 295 GG + S+ L+E + AYD GILI+A+AGN G G +T+ YPA+YS+ IAV ++D +R Sbjct: 228 GGASESEALKEAVDRAYDKGILIVASAGNAGSYGSLNTVDYPAKYSSVIAVASVDQRKQR 287 Query: 296 AFFSAKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEV 355 AF S+ G ++++ APGV LST+P N+YG KSGTSMA+PHV G AA+IL K+ L+ EV Sbjct: 288 AFDSSVGEEVEVSAPGVSTLSTIPHNEYGYKSGTSMASPHVAGAAAVILSKHPNLTNDEV 347 Query: 356 ADILESTAEPLGVVPEYGHGIVQAKEA 382 + L TA LG YG G+V ++A Sbjct: 348 RERLSKTATQLGDPFYYGAGLVNVQKA 374 >B6VFQ8.1 B6VFQ8_BACSU Subtilisin (Fragment) Length = 381 Score = 278 bits (710), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 166/383 (43%), Positives = 228/383 (59%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E E +++PYGI Sbjct: 66 VQKQFKYVNAATATLDEKAVKELKQDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ VK G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVKGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG + Sbjct: 177 TIAALNNTIGVLGVAPNASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPS 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGSSFYYGKGLINVQAA 379 >A0FLP3.1 A0FLP3_BACSU Subtilisin Length = 381 Score = 277 bits (709), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 167/383 (43%), Positives = 230/383 (60%), Gaps = 15/383 (3%) Query: 4 IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62 I LL + LI M S ++A + S K Y + ++++++ DVI + GGK Sbjct: 8 ISLLFALTLIFTMAFSN--MSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65 Query: 63 LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122 ++K + + A L ++ ELK P V Y+E ++ + E +SV PYGI Sbjct: 66 VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDH-----IAHEYAQSV----PYGISQ 116 Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180 I A +GYTG VK+AV+DSG S H D+ V+ G SF+ + N Y D HGTHVAG Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176 Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240 IAA N GV+GVAP A +YA+KVLD G G Y +I GI+WAI + MD+IN+S GG Sbjct: 177 TIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPT 236 Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299 S L+ + +A +GI++ AAAGN+G G T+ YPA+Y + IAVGA++SSN+RA FS Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296 Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359 + G +LD++APGV + STLP YG +GTSMA PHV G AALIL K+ + +V D L Sbjct: 297 SAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382 ESTA LG YG G++ + A Sbjct: 357 ESTATYLGSSFYYGKGLINVQAA 379 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,384,193,355 Number of extensions: 103920987 Number of successful extensions: 238096 Number of sequences better than 1.0e-05: 3793 Number of HSP's gapped: 229767 Number of HSP's successfully gapped: 3936 Length of query: 389 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 251 Effective length of database: 1,760,596,133 Effective search space: 441909629383 Effective search space used: 441909629383 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)