BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0021.p (450 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D536.1 B1D536_9BACL MATE efflux family protein 783 0.0 A9VUN4.1 A9VUN4_BACWK MATE efflux family protein 752 0.0 Q49VC5.1 MEPA_STAS1 Multidrug export protein mepA 410 e-112 C2K265.1 C2K265_STAAU MATE family multi-antimicrobial extrusion ... 399 e-109 C2G6J9.1 C2G6J9_STAAU MATE family multi-antimicrobial extrusion ... 399 e-109 >B1D536.1 B1D536_9BACL MATE efflux family protein Length = 450 Score = 783 bits (2021), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/450 (86%), Positives = 417/450 (92%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 MDA+NL+YFEKAPVAKAVAHFAVPMMLGTSM+VIYSILNAYFLG Sbjct: 1 MDAQNLHYFEKAPVAKAVAHFAVPMMLGTSMSVIYSILNAYFLGTLNNTAMLTALALTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 +IMALGNLIGMGSGTFISRLLGE++YDDVKHVSSFAFYSSLVLGLI++AVGLPLI+ Sbjct: 61 LFAVIMALGNLIGMGSGTFISRLLGERKYDDVKHVSSFAFYSSLVLGLIVIAVGLPLIDP 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 IVHGLGATP+SFGFTKDYVT+MLIGSP VVLFFTLEN+VRSEGAA+TSM GMILSVVVNI Sbjct: 121 IVHGLGATPESFGFTKDYVTIMLIGSPIVVLFFTLENIVRSEGAAITSMTGMILSVVVNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILDALV++VFHWDVIGVASATV+SNLVA +++AFHMG KSQFLTIS+KWFKATK+I+ NV Sbjct: 181 ILDALVVYVFHWDVIGVASATVVSNLVACVFFAFHMGKKSQFLTISMKWFKATKEIMSNV 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIGVPVFIMSIFLGAMSLIFNH+LVEYGD +VAAYGISSRLLQFPEFILMGLCEGVVPL Sbjct: 241 FKIGVPVFIMSIFLGAMSLIFNHYLVEYGDQSVAAYGISSRLLQFPEFILMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IAF+FTA+K RMK TIGFTIK IV LAVVFGI+VYLISDHLIGLFTNDPQLIEMGSYILH Sbjct: 301 IAFTFTANKSRMKETIGFTIKMIVSLAVVFGIIVYLISDHLIGLFTNDPQLIEMGSYILH 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VTFLSLFITGMTTLFTG+FQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV Sbjct: 361 VTFLSLFITGMTTLFTGIFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IAD AFLVGAVMLY+LRNKLQP+LESL Q Sbjct: 421 IADTVAFLVGAVMLYILRNKLQPDLESLAQ 450 >A9VUN4.1 A9VUN4_BACWK MATE efflux family protein Length = 450 Score = 752 bits (1941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/450 (83%), Positives = 408/450 (90%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 MDAENLYYFEKA V+KAVAHFAVPMMLGTSM+VIYSILNAYFLG Sbjct: 1 MDAENLYYFEKASVSKAVAHFAVPMMLGTSMSVIYSILNAYFLGTLHSTAMLTALALTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 IIMALGNLIGMGSGTFISRLLGEK+YDDVKHVSSFAFYSSLVLGLI +AVGLPLI+ Sbjct: 61 LFAIIMALGNLIGMGSGTFISRLLGEKKYDDVKHVSSFAFYSSLVLGLIAIAVGLPLIDQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 IV+GLGAT DSF FTKDYVT+M+IGSPFV+LFFTLEN+VRSEGAA+TSMIGMILSVVVNI Sbjct: 121 IVYGLGATSDSFEFTKDYVTIMIIGSPFVILFFTLENIVRSEGAAITSMIGMILSVVVNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD+LVIFVFHW V GVASATVIS++VAS +YAFH+ KSQFLT+S KWFK TK+IL NV Sbjct: 181 ILDSLVIFVFHWGVTGVASATVISSVVASAFYAFHIRYKSQFLTVSAKWFKVTKEILSNV 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIGVPVFIMSIFLGAMSLI NHFL+EYGD AVAAYGISSRLLQFPEFILMGLCEG+VPL Sbjct: 241 FKIGVPVFIMSIFLGAMSLILNHFLIEYGDQAVAAYGISSRLLQFPEFILMGLCEGIVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IAFSFTA+K+RMK+TIGFTIK IV LA VFG++VYLISD+LIGLFTNDP LIEMGSYILH Sbjct: 301 IAFSFTANKMRMKHTIGFTIKTIVALAFVFGVIVYLISDNLIGLFTNDPHLIEMGSYILH 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VTFLSLFITGMT LFTG+FQATAQGTAAFIMSIIQG+TLIPVL+IAN M GFHGV+WSLV Sbjct: 361 VTFLSLFITGMTALFTGIFQATAQGTAAFIMSIIQGITLIPVLFIANHMYGFHGVIWSLV 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IADA +FLVGA+MLYVLRNKLQP+L+SLVQ Sbjct: 421 IADAVSFLVGAIMLYVLRNKLQPDLDSLVQ 450 >Q49VC5.1 MEPA_STAS1 Multidrug export protein mepA Length = 451 Score = 410 bits (1053), Expect = e-112, Method: Compositional matrix adjust. Identities = 212/450 (47%), Positives = 300/450 (66%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M E LYYFEK+ V K + HF+VPMM+G+ ++VIY ILN YF+G Sbjct: 1 MKDEQLYYFEKSSVFKGMMHFSVPMMIGSLLSVIYGILNIYFIGFLGDSHMISAISLTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 ++M LGNL G+G GT+ISRLLG K Y K VSSF+ Y L+LG+I++ + + E Sbjct: 61 IFAVLMGLGNLFGIGGGTYISRLLGAKDYVKTKFVSSFSIYGGLILGVIVILMTIFATEQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I H LGA ++ GFT Y+ VM + +PFV+LFF LE RS GA + SMIGM+ SVV+NI Sbjct: 121 IAHLLGARGETLGFTSAYLKVMFLSAPFVILFFILEQFARSIGAPIISMIGMLASVVLNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD ++IF F +V+G A T ISN+VAS+++ + KS +T+S+ + + TK+IL + Sbjct: 181 ILDTILIFGFDLNVVGAALGTAISNVVASLFFIIYFLRKSDIITMSLSYVRPTKEILVEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FK+G P F+MSI +G L+ N FL YG+ A+A+YGIS RL+QFPE I+MGLCEGVVPL Sbjct: 241 FKVGFPAFLMSILMGVTGLVLNLFLAGYGNFAIASYGISFRLVQFPELIIMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IA++F +DK RMKN I I IV + V ++V+ LIG+F+ND ++ M +++L Sbjct: 301 IAYNFMSDKARMKNIIKVVIMTIVVIFAVCMVIVFTTGHQLIGIFSNDAAIVGMATFMLK 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VT SL + G+ LFTG+ QAT QG A IM+I+QGV +IPVL++ N + G G+VWSL+ Sbjct: 361 VTMTSLLLNGIGFLFTGMLQATGQGRGATIMAILQGVVIIPVLFVMNGLFGLTGIVWSLL 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IA+ L +++Y+LR++L + L++ Sbjct: 421 IAETICALAAMLIVYLLRDRLTVDKAELLE 450 >C2K265.1 C2K265_STAAU MATE family multi-antimicrobial extrusion protein Length = 451 Score = 399 bits (1025), Expect = e-109, Method: Compositional matrix adjust. Identities = 212/450 (47%), Positives = 301/450 (66%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M E LYYFEK+PV KA+ HF++PMM+GT ++VIY ILN YF+G Sbjct: 1 MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIGFLEDSHMISAISLTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+M LGNL G+G+GT+ISRLLG K Y K VSSF+ Y + LGLI++ V LP + Sbjct: 61 VFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYGGIALGLIVILVALPFSDQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I LGA ++ T +Y+ VM + +PFV+LFF LE R+ GA M SMIGM+ SV +NI Sbjct: 121 IAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFARAIGAPMISMIGMLASVGLNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD ++IF F +V+G A T ISN+ A++++ + S +++++K K K++L + Sbjct: 181 ILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMKNSDVVSVNIKLAKPNKEMLSEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIG+P F+MSI +G L+ N FL YG+ A+A+YGIS RL+QFPE I+MGLCEGVVPL Sbjct: 241 FKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASYGISFRLVQFPELIIMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IA++F ++K RMK+ I I +I + VV I V+ I H++GLFT D ++EM ++IL Sbjct: 301 IAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAVFTIGHHMVGLFTTDQAIVEMATFILK 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VT SL + G+ LFTG+ QAT QG A IM+I+QGV +IPVL+I N + G GV+WSL+ Sbjct: 361 VTMTSLLLNGIGFLFTGMLQATGQGRGATIMAILQGVVIIPVLFIMNALFGLTGVIWSLL 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IA++ L +++Y+LR++L + L++ Sbjct: 421 IAESLCALAAMLIVYLLRDRLTVDTSELIE 450 >C2G6J9.1 C2G6J9_STAAU MATE family multi-antimicrobial extrusion protein Length = 451 Score = 399 bits (1025), Expect = e-109, Method: Compositional matrix adjust. Identities = 212/450 (47%), Positives = 301/450 (66%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M E LYYFEK+PV KA+ HF++PMM+GT ++VIY ILN YF+G Sbjct: 1 MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIGFLEDSHMISAISLTLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+M LGNL G+G+GT+ISRLLG K Y K VSSF+ Y + LGLI++ V LP + Sbjct: 61 VFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYGGIALGLIVILVALPFSDQ 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I LGA ++ T +Y+ VM + +PFV+LFF LE R+ GA M SMIGM+ SV +NI Sbjct: 121 IAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFARAIGAPMISMIGMLASVGLNI 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 ILD ++IF F +V+G A T ISN+ A++++ + S +++++K K K++L + Sbjct: 181 ILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMKNSDVVSVNIKLAKPNKEMLSEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 FKIG+P F+MSI +G L+ N FL YG+ A+A+YGIS RL+QFPE I+MGLCEGVVPL Sbjct: 241 FKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASYGISFRLVQFPELIIMGLCEGVVPL 300 Query: 301 IAFSFTADKLRMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 IA++F ++K RMK+ I I +I + VV I V+ I H++GLFT D ++EM ++IL Sbjct: 301 IAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAVFTIGHHMVGLFTTDQAIVEMATFILK 360 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 VT SL + G+ LFTG+ QAT QG A IM+I+QGV +IPVL+I N + G GV+WSL+ Sbjct: 361 VTMTSLLLNGIGFLFTGMLQATGQGRGATIMAILQGVVIIPVLFIMNALFGLTGVIWSLL 420 Query: 421 IADAAAFLVGAVMLYVLRNKLQPELESLVQ 450 IA++ L +++Y+LR++L + L++ Sbjct: 421 IAESLCALAAMLIVYLLRDRLTVDTSELIE 450 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.143 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,305,529,007 Number of extensions: 90124069 Number of successful extensions: 445524 Number of sequences better than 1.0e-05: 3428 Number of HSP's gapped: 439134 Number of HSP's successfully gapped: 3435 Length of query: 450 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 311 Effective length of database: 1,751,175,118 Effective search space: 544615461698 Effective search space used: 544615461698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)