BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0017.p (579 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K405.1 B3K405_9BACI Histidine kinase internal region 224 3e-56 Q65NG8.1 Q65NG8_BACLD Putative uncharacterized protein 217 3e-54 Q62YW8.1 Q62YW8_BACLD Putative Histidine kinase 217 3e-54 B1D616.1 B1D616_9BACL Integral membrane sensor signal transducti... 214 2e-53 C5EQ73.1 C5EQ73_9FIRM Predicted protein 214 3e-53 >B3K405.1 B3K405_9BACI Histidine kinase internal region Length = 606 Score = 224 bits (570), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 167/577 (28%), Positives = 277/577 (48%), Gaps = 38/577 (6%) Query: 23 CSLLPLVLIASLTFVYLSKVIENKMSDTTSNLLGSINWNIQTFVNDVESISKLMLSSRDV 82 +L+PL+ + +F + E K T N L + + T V DVE+IS ++ V Sbjct: 20 ATLVPLLFLGIFSFNIAKSLTEEKAKITGMNTLRQLEAYLDTMVKDVENISLFLIGHTGV 79 Query: 83 QNFLSYTKNDFKEIYDLQNATRSLIVNITNNKSYIRYVYVGS-GQRELITTNQWDRLTYD 141 Q +L ++++ + Q + + N+ +K YI + V G ++ I+ R + Sbjct: 80 QAYLKTEQSNYVQ----QTTVINFLTNLAFSKDYIANIIVEPLGSKDAISHKSLVRSEFR 135 Query: 142 NIYQAVSRTTWYQQVGEMGGRGLWLNSDEVRLLKGSPNLLLYGKRLNNLETLEPIGMLII 201 NI + V +Y+ + W +G ++ + + + + +PIG L I Sbjct: 136 NITEMVP--DYYEHHPKW-----WSFVHRQWTFEGVRKVITLARPIRSTDKYKPIGNLQI 188 Query: 202 SIDRRVFDEMFKNISNAEKGDLMILEQNKIIYSNSGNNDLKKVPQKDLNQIYDLPE-RGN 260 ++D+ V F+ + G +++L++N I + + + I + GN Sbjct: 189 NLDQGVIANQFRQAVLEKTGFVLLLDENNRIIAGPPDMETNLTLADYYPAIGEFEGLSGN 248 Query: 261 RIETMGGERYVITFETNPVTQWKVVSMIP---YSSINSEIILIRNVTLSLTLVAFLLAAY 317 G + I ++ WK+V +IP Y S N I + V ++ VA LLA Sbjct: 249 IDYGEGSSKKTILYKKMSSVNWKLVGIIPSQEYRSQNQYFIKLTAVAVT---VAILLAII 305 Query: 318 GAYLISKRITKQLTLLTSVARKAAKRDEIEGILFVEEDEIGKIGNQFLRTFRTNTELSDX 377 + ++IT L++LT + ++ + + + DE+G++ + R +L+D Sbjct: 306 LVLFLIQKITNPLSVLTKFLKNSSPEEPLPALPVKTVDEVGQLIISYNRLSSRIVKLTDE 365 Query: 378 XXXXXXXXXXXXXXXXQSQINPHFLYNTLNSIYLMAERISAKNISKMVMSLSNIFKLSLN 437 Q QINPHFLYNTL+SI+ +A I++MV SLS+ + SLN Sbjct: 366 VKLNESLKKEADMHALQVQINPHFLYNTLSSIHWLALMNQDVKIAEMVGSLSDFLRFSLN 425 Query: 438 NGDYITTVGNEIEQVKNYLEIQNIRYKGKFEVIIDLEPGIEHIRMLKLLIQPLVENAIFH 497 NG T+ EI V++Y+ IQ IRY KFE +E + MLKLL+QPLVEN++ H Sbjct: 426 NGQEYCTIQQEIMHVRHYVNIQTIRYPDKFEFKDLVEEDLYQHTMLKLLLQPLVENSMLH 485 Query: 498 GIELKEGKGSIVIRGRREGAILIFEVEDDGVGF--------------DPVDMQ----PRG 539 GI ++G G+I IRG RE + F VEDDG+G +P+ + PRG Sbjct: 486 GILSRDGVGTITIRGHREPEGIRFVVEDDGIGMTAERLKWLQEQLVENPLQYEKGQTPRG 545 Query: 540 -YALRNIQERIQLHYGQDRGIRIDSIPGAGTKITLEI 575 Y LRN+ +R+ LHYG+D G+R++S G GT++ I Sbjct: 546 SYGLRNVHKRLLLHYGKDAGLRVESTEGTGTRVMFTI 582 >Q65NG8.1 Q65NG8_BACLD Putative uncharacterized protein Length = 594 Score = 217 bits (553), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 168/581 (28%), Positives = 286/581 (49%), Gaps = 56/581 (9%) Query: 27 PLVLIASLTFVYLSKVIENKMSDTTSNLLGSINWNIQTFVNDVESISKLMLSSRDVQNFL 86 P + + L+ + +IE ++ D T L+G +N + +V+ +++++ L+ + +++ F Sbjct: 33 PTIGLGILSGWAVENIIEKQVIDQTLQLIGEVNKTAEVYVSHMQNLTYLISMNEEMEAFF 92 Query: 87 SYTKNDFKEIYDLQNATRSLIVNITNNKSYIRYVYVGSGQRELITTNQWDRLTYDNIYQA 146 S+ K D + Y R+ + +T+ S + V + + E+I+ ++R D Sbjct: 93 SHKKEDGEADY----KRRTFLQGLTSLYSEAAGILVVNDKGEMISNEMYERTPTD----- 143 Query: 147 VSRTTWYQQVGEMGGRGLWLNSDEVRLLKGSP--------------NLLLYGKRLNNLET 192 +++ WYQ L+++ + + G P ++ + + + ET Sbjct: 144 LTKEPWYQAA---------LDNEGIFKMIGKPVNRNIRSHVHYREDEVVSAVRAVIDPET 194 Query: 193 LEPIGMLIISIDRRVFDEMFKNISNAEKGDLMILEQNKI-IYSNSGNNDLKKVPQKDLNQ 251 G+++I + RV E KNI + G LMI+++N IYS +N +P+K Q Sbjct: 195 QHVKGVVLIDLKLRVLAEATKNIRLGKTGYLMIMDENGGNIYSPETSNLF--LPKKWFRQ 252 Query: 252 IYDLPER-GNRIETMGGERYVITFETNPVTQWKVVSMIPYSSINSEIILIRNVTLSLTLV 310 ER G + + GE Y +ET+ T W V + E+ I L L Sbjct: 253 -----ERSGYFSKRIKGEEYQFIYETSSFTNWTTVGVFKAGEPVFEVKEIHLYVLLFLLT 307 Query: 311 AFLLAAYGAYLISKRITKQLTLLTSVARKAAKRDEIEGILFVEE--DEIGKIGNQFLRTF 368 A L +Y +S +++ + L +V + A + L+ EE DEIG +G F R Sbjct: 308 AAFLGMSASYYLSHSMSRPILKLNAVMKTAESGNF--STLYQEEREDEIGLLGKSFNRLM 365 Query: 369 RTNTELSDXXXXXXXXXXXXXXXXXQSQINPHFLYNTLNSIYLMAERISAKNISKMVMSL 428 EL Q+QINPHFLYNTL++I MA + A+ +S +V +L Sbjct: 366 FRIRELMSVTERQERQKREAELRALQAQINPHFLYNTLDTINWMARKKGAEEVSDLVQAL 425 Query: 429 SNIFKLSLNNGDYITTVGNEIEQVKNYLEIQNIRYKGKFEVIIDLEPGIEHIRMLKLLIQ 488 S +F++SL+ G I + +E E V+NYL+IQ RY+ K I++ + +LKL++Q Sbjct: 426 SKLFRISLSKGKDIIPLSDEFEHVENYLKIQKARYRDKLNYSIEMTGLCDDRLILKLVLQ 485 Query: 489 PLVENAIFHGIELKEGKGSIVIRGRREGAILIFEVEDDGVGFDPVDMQ-----------P 537 P+VENAI+HGI+ K+G G I I+G E LI ++EDDG G D ++ Sbjct: 486 PIVENAIYHGIKEKKGPGHIAIKGMEEDGSLIIKIEDDGAGMDEETLKQITDQFKHRDDT 545 Query: 538 RGYALRNIQERIQLHYGQDRGIRIDSIPGAGTKITLEIGIM 578 GY + N+ ERI L +G+ GI ++S GAGT +T+ + I+ Sbjct: 546 AGYGMINVHERIALTFGEPYGITVESRKGAGTAVTIRLPIL 586 >Q62YW8.1 Q62YW8_BACLD Putative Histidine kinase Length = 589 Score = 217 bits (552), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 168/581 (28%), Positives = 286/581 (49%), Gaps = 56/581 (9%) Query: 27 PLVLIASLTFVYLSKVIENKMSDTTSNLLGSINWNIQTFVNDVESISKLMLSSRDVQNFL 86 P + + L+ + +IE ++ D T L+G +N + +V+ +++++ L+ + +++ F Sbjct: 28 PTIGLGILSGWAVENIIEKQVIDQTLQLIGEVNKTAEVYVSHMQNLTYLISMNEEMEAFF 87 Query: 87 SYTKNDFKEIYDLQNATRSLIVNITNNKSYIRYVYVGSGQRELITTNQWDRLTYDNIYQA 146 S+ K D + Y R+ + +T+ S + V + + E+I+ ++R D Sbjct: 88 SHKKEDGEADY----KRRTFLQGLTSLYSEAAGILVVNDKGEMISNEMYERTPTD----- 138 Query: 147 VSRTTWYQQVGEMGGRGLWLNSDEVRLLKGSP--------------NLLLYGKRLNNLET 192 +++ WYQ L+++ + + G P ++ + + + ET Sbjct: 139 LTKEPWYQAA---------LDNEGIFKMIGKPVNRNIRSHVHYREDEVVSAVRAVIDPET 189 Query: 193 LEPIGMLIISIDRRVFDEMFKNISNAEKGDLMILEQNKI-IYSNSGNNDLKKVPQKDLNQ 251 G+++I + RV E KNI + G LMI+++N IYS +N +P+K Q Sbjct: 190 QHVKGVVLIDLKLRVLAEATKNIRLGKTGYLMIMDENGGNIYSPETSNLF--LPKKWFRQ 247 Query: 252 IYDLPER-GNRIETMGGERYVITFETNPVTQWKVVSMIPYSSINSEIILIRNVTLSLTLV 310 ER G + + GE Y +ET+ T W V + E+ I L L Sbjct: 248 -----ERSGYFSKRIKGEEYQFIYETSSFTNWTTVGVFKAGEPVFEVKEIHLYVLLFLLT 302 Query: 311 AFLLAAYGAYLISKRITKQLTLLTSVARKAAKRDEIEGILFVEE--DEIGKIGNQFLRTF 368 A L +Y +S +++ + L +V + A + L+ EE DEIG +G F R Sbjct: 303 AAFLGMSASYYLSHSMSRPILKLNAVMKTAESGNF--STLYQEEREDEIGLLGKSFNRLM 360 Query: 369 RTNTELSDXXXXXXXXXXXXXXXXXQSQINPHFLYNTLNSIYLMAERISAKNISKMVMSL 428 EL Q+QINPHFLYNTL++I MA + A+ +S +V +L Sbjct: 361 FRIRELMSVTERQERQKREAELRALQAQINPHFLYNTLDTINWMARKKGAEEVSDLVQAL 420 Query: 429 SNIFKLSLNNGDYITTVGNEIEQVKNYLEIQNIRYKGKFEVIIDLEPGIEHIRMLKLLIQ 488 S +F++SL+ G I + +E E V+NYL+IQ RY+ K I++ + +LKL++Q Sbjct: 421 SKLFRISLSKGKDIIPLSDEFEHVENYLKIQKARYRDKLNYSIEMTGLCDDRLILKLVLQ 480 Query: 489 PLVENAIFHGIELKEGKGSIVIRGRREGAILIFEVEDDGVGFDPVDMQ-----------P 537 P+VENAI+HGI+ K+G G I I+G E LI ++EDDG G D ++ Sbjct: 481 PIVENAIYHGIKEKKGPGHIAIKGMEEDGSLIIKIEDDGAGMDEETLKQITDQFKHRDDT 540 Query: 538 RGYALRNIQERIQLHYGQDRGIRIDSIPGAGTKITLEIGIM 578 GY + N+ ERI L +G+ GI ++S GAGT +T+ + I+ Sbjct: 541 AGYGMINVHERIALTFGEPYGITVESRKGAGTAVTIRLPIL 581 >B1D616.1 B1D616_9BACL Integral membrane sensor signal transduction histidine kinase Length = 588 Score = 214 bits (544), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 156/564 (27%), Positives = 287/564 (50%), Gaps = 37/564 (6%) Query: 24 SLLPLVLIASLTF-VYLSKVIENKMSDTTSNLLGSINWNIQTFVNDVESISKLMLSSRDV 82 S+ PL+++ ++F + + ++ N + + NL + F N V ++ +L+L++ + Sbjct: 30 SVPPLIILGYISFNISKNTIVHNHLDNNKHNLQAASEVADLLFRN-VINMHRLILANDQL 88 Query: 83 QNFLSYTKNDFKEIYDLQNATR--SLIV-NITNNKSYIRYVYVGSGQRELITTN-QWDRL 138 ++ L + D + D++ A R +L+V N+ + + G + + + + Sbjct: 89 RDELKASAADESHVLDIRTANRLQNLVVRNLIDTRHIDSICLFDRGFHSVCYGSVEANAG 148 Query: 139 TYDN--IYQAVSRTTWYQQVGEMGGRGLWLNSDEVRLLKGSPNLLLYG--KRLNNLETLE 194 YD+ + +T WY++ GR ++ + + ++ + + K L + Sbjct: 149 IYDDETSRSVIGQTDWYKRAEAANGREVFFSYNVLQSINSGNAGTSFSSVKLLRDPANFR 208 Query: 195 PIGMLIISIDRRVFDEMFKNISNAEKGDLMILEQ----NKIIYSNSGNNDLKKVPQKDLN 250 IG+LII++ + +F++ + GD ++L+ ++ +Y+ N ++ + +++ Sbjct: 209 VIGLLIINLKKTIFEQAM-----GDDGDFIVLDTTGKFSRSVYAQ--NASIEHLIDREVG 261 Query: 251 Q---IYDLPERGNRIETMGGERYVITFETNPVTQWKVVSMIPYSSINSEIILIRNVTLSL 307 + + +L + G Y+ N T W + ++ + + + I VT+ + Sbjct: 262 EDSVLRELHQHG----------YLTVGYRNATTNWTFLHIVKTNVLLQDSNRIGTVTMLI 311 Query: 308 TLVAFLLAAYGAYLISKRITKQLTLLTSVARKAAKRD--EIEGILFVEEDEIGKIGNQFL 365 L+A + +S IT+ L L + +K ++ I F EEDE+G IG F Sbjct: 312 AGFIALIAIILSMFVSGSITRPLLQLKKMMVDWSKGSGASVQVISF-EEDEVGAIGETFR 370 Query: 366 RTFRTNTELSDXXXXXXXXXXXXXXXXXQSQINPHFLYNTLNSIYLMAERISAKNISKMV 425 + N +L++ Q+QI PHFLYNTL+SIY MA + +I+KM Sbjct: 371 KISSENKQLNERLIHSQLKEREAELRTLQAQIKPHFLYNTLDSIYWMAILQNNADIAKMT 430 Query: 426 MSLSNIFKLSLNNGDYITTVGNEIEQVKNYLEIQNIRYKGKFEVIIDLEPGIEHIRMLKL 485 ++LS FK+SLN G V E++ +++Y+ IQN+RYK +F I+++E G+ I +LKL Sbjct: 431 VALSESFKISLNKGKETIPVFKELQHIEHYMTIQNLRYKSRFHYIVNVEQGLMSIEILKL 490 Query: 486 LIQPLVENAIFHGIELKEGKGSIVIRGRREGAILIFEVEDDGVGFDPVDMQPRGYALRNI 545 ++QPLVENAI+HG+E K G+GSI + GR E +F VEDDGVG + + GY L N+ Sbjct: 491 ILQPLVENAIYHGLEPKVGEGSIRLTGRMEDGTAVFTVEDDGVGMEDIQATETGYGLVNV 550 Query: 546 QERIQLHYGQDRGIRIDSIPGAGT 569 +ERI L+YG + + S PG GT Sbjct: 551 RERIMLYYGHRSSLTVRSEPGKGT 574 >C5EQ73.1 C5EQ73_9FIRM Predicted protein Length = 574 Score = 214 bits (544), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 164/574 (28%), Positives = 284/574 (49%), Gaps = 54/574 (9%) Query: 28 LVLIASLTFVYLSKVIENKMSDTTSNLLGSINWNIQTFVNDVESISKLMLSSRDVQNFLS 87 ++L+ + + S ++ S++ +L S + + +++ I++ +L+ +QN L+ Sbjct: 29 VILLGVMIYGMTSAMLIKTNSESYLKVLESADIILSHYLDSYRDIARNVLADPVLQNDLT 88 Query: 88 YTKN--------DFKEIYDLQNATRSLIVNITNNKSYIRYVYVGSGQRELITTNQWDRLT 139 + N + + DL NA I+ + +S Y+Y G+ + + R Sbjct: 89 ISSNSRSGGRRIEERLFVDLDNAMEKYIMGFSGARSI--YLYDSRGRLFYLDDSGQGRSA 146 Query: 140 YDNI-YQAVSRTTWYQQVGEMGGRGLWLNSDEVRLLKGSPNLLLYGKRLNNLETLEPIGM 198 D Y +S T W+++ G + + ++ G+ K L NL+T + IGM Sbjct: 147 ADAADYGVISTTPWFKKAAAQKGYEGFAGRN---VITGNREQFSCIKLLKNLDTQDVIGM 203 Query: 199 LIISIDRRVFDEMFKNISNAEKGDLMILEQNK-----IIYSNSGNNDLKKVPQKDLNQIY 253 +I++ D+ E+F + E+G ++EQ + ++ S+ + +++ Sbjct: 204 MILNFDKAALKEVFPSY-RPEQGAYAVVEQQEDGGYDVVLSSGAAMEEQEIA-------- 254 Query: 254 DLPERGNRIETMGGERYVITFETNPVTQWKVVSMIPYSSINSEIILIRNVTLSLTLVAFL 313 RI G Y ++ + W++V ++ ++ SE I+ + ++ + + Sbjct: 255 -------RILVKGDPDYHLSRYGSSEMDWELVHLVQNRNLLSEAGKIQGIIMNGLAITIV 307 Query: 314 LAAYGAYLISKRITKQLTLLTSVARKAAKRDEI----EGILFVE----EDEIGKIGNQFL 365 L L+ RITK L L RD+I EG +E +DEIG IG +F Sbjct: 308 LLLVLTVLLCNRITKPLYQL---------RDDIVRVGEGERCLETDFPDDEIGAIGQEFS 358 Query: 366 RTFRTNTELSDXXXXXXXXXXXXXXXXXQSQINPHFLYNTLNSIYLMAERISAKNISKMV 425 R L+D QS INPHFLYNTL+S+Y MA A++++++ Sbjct: 359 RMVNEKLTLADQVTRVELKNKEAELELLQSNINPHFLYNTLDSLYWMAILHEAEDVAELT 418 Query: 426 MSLSNIFKLSLNNGDYITTVGNEIEQVKNYLEIQNIRYKGKFEVIIDLEPGIEHIRMLKL 485 +LS++FK++L+ G+ + E++ V++YL IQNIR++GK V I + + +++KL Sbjct: 419 KALSDVFKIALSKGEKFIPIQKELDFVRSYLYIQNIRFEGKIHVDIQADESLMGYKVIKL 478 Query: 486 LIQPLVENAIFHGIELKEGKGSIVIRGRREGAILIFEVEDDGVGFDPVDMQPRGYALRNI 545 L+QP VENA++HG+E K G G++VI+ RE L FEV DDGVG D +GYALRN Sbjct: 479 LLQPFVENAVYHGLEPKLGSGTVVIKVYREQDFLCFEVADDGVGMDVDASLSKGYALRNS 538 Query: 546 QERIQLHYGQDRGIRIDSIPGAG--TKITLEIGI 577 ERI+L YG D + S PG G KI+L +G+ Sbjct: 539 LERIRLVYGDDAKVEFKSSPGQGVQVKISLRVGV 572 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,016,401,502 Number of extensions: 124295913 Number of successful extensions: 372062 Number of sequences better than 1.0e-05: 2197 Number of HSP's gapped: 369264 Number of HSP's successfully gapped: 2197 Length of query: 579 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 438 Effective length of database: 1,732,333,088 Effective search space: 758761892544 Effective search space used: 758761892544 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)