BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0013.p (486 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein 437 e-120 C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase 429 e-118 A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 pro... 425 e-117 C4GDD6.1 C4GDD6_9FIRM Putative uncharacterized protein 422 e-116 C4ZAM5.1 C4ZAM5_9FIRM Putative beta-fructofuranosidase protein 389 e-106 >B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein Length = 474 Score = 437 bits (1124), Expect = e-120, Method: Compositional matrix adjust. Identities = 221/496 (44%), Positives = 306/496 (61%), Gaps = 40/496 (8%) Query: 1 LTNLFYKPDGAWVGDLIPFYKDGSFRLFYLHDWRDNKKIHGEGTSWFELHTSDFVHYEEV 60 + FY+P+ AWVGD+IP+Y+DG F+LFYLH WRDN + G W+ + T DFV+Y E Sbjct: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYR-EGLDHGWYLVGTKDFVNYREE 59 Query: 61 GEKLKHGSVSEQDLNCYTGSIIEAHGEYHLFYTGLNPNPEFIEDGVPLQCVMHAVSTDME 120 G G TG I++ G YH+FY P Q V HA+S D++ Sbjct: 60 GPCKIEGG---------TGHILKVDGIYHMFYCIFPPGK---------QLVCHAISRDLK 101 Query: 121 SWRKIPEDTFYSDGIQYERHDWRDPFVFWNEEAGEYWMLLAARNKEGPSRRRGSIGLCAS 180 +W IPEDTF +D YE DWRDPFVFWNEE G+YWMLL+A K GP+ R+G L +S Sbjct: 102 TWEPIPEDTFAADDRIYELSDWRDPFVFWNEEEGQYWMLLSAMAK-GPTNRKGCTALLSS 160 Query: 181 KDLKSWEIRQPFWNPKMYV-THECPDLFQIGEWWYLVYSTFSERFVTHYRMSKSLSGPWT 239 KDLK+WE R+P + P ++V HECPDLF+IG+WWYL+YS+++ RF T YRMS+SL+GPW Sbjct: 161 KDLKAWEYREPLYAPNLHVGAHECPDLFRIGDWWYLIYSSYTGRFATFYRMSRSLNGPWI 220 Query: 240 APRNDAFDARAFYAAKTWSDGNKRYAFGWDPSKENE-----------NDYGDWQWAGNLV 288 P+ D FD RAFYAAK+ +DG KRY FGW+P+K ++ DY W W GNLV Sbjct: 221 TPKEDTFDGRAFYAAKSVADGQKRYLFGWNPTKNDDLFGWNPPKSQGKDYDTWDWGGNLV 280 Query: 289 VHELVQEQDGTLTVRIPETVSHHFSNKQAAKWDSRIGEWEESEGRIETAANETYS-CISA 347 VHE++Q DGTL V++P+TV F +++ G W +S+ + + ++ C + Sbjct: 281 VHEIIQRPDGTLGVKVPDTVDAAFKELMPVRFNRVEGAWLDSDCSLSCESPYGFAGCTTR 340 Query: 348 GFMPETCKISAELSFSEGTRGCGILLRASEDLDEAYYIRLEPIHNRLVFDMW-PRREQGE 406 +P+ CKISA +SFS T+G G +LR + LD AYYI EP NRL F + + E+G Sbjct: 341 EDLPDQCKISASVSFSGQTQGLGFMLRTEDTLDAAYYITFEPQRNRLTFRGYIMQSEEGG 400 Query: 407 MQWHIKGDHPHAVELEVPIELQAGTVYNIEIVVENSVCVVYLADQVAMTTRLYNLKTGSW 466 + P+ VELE PIEL Y I++ ++ S+C +Y+ + +AM+ R+Y+++ G Sbjct: 401 KTF------PYEVELERPIELIPERPYEIKVFIDGSICEIYVDENIAMSARMYDIQHGKL 454 Query: 467 GCFVQEGRAVFEQASI 482 G FV +G A F I Sbjct: 455 GLFVSQGSAQFSDVRI 470 >C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase Length = 482 Score = 429 bits (1104), Expect = e-118, Method: Compositional matrix adjust. Identities = 220/479 (45%), Positives = 296/479 (61%), Gaps = 16/479 (3%) Query: 1 LTNLFYKPDGAWVGDLIPFYKDGSFRLFYLHDWR--DNKKIHGEGTSWFELHTSDFVHYE 58 + N+FYKP WVGD IPF DG F +FYLHD R + + +G TSW L T D V+ + Sbjct: 1 MKNIFYKPGDGWVGDTIPFAHDGKFYIFYLHDERKGNTEDEYGYRTSWNLLITEDGVNIK 60 Query: 59 EVGEKLKHGSVSEQDLNCYTGSIIEAH-GEYHLFYTGLNP-NPEFIEDGVPLQCVMHAVS 116 + L G + D CYTGS+IE + ++H+FYT N NP++ +G PLQ V HA+S Sbjct: 61 DCKVVLPVGGYDDADYACYTGSVIEGNDSKFHMFYTAQNNYNPKYHRNGRPLQYVAHAIS 120 Query: 117 TDMESWRKIPEDTFYSDGIQYERHDWRDPFVFWNEEAGEYWMLLAARNKEGPSRRRGSIG 176 D+ W K+PE TF +D YE DWRDPFVF+NEE + MLLAAR + + G +G Sbjct: 121 EDLIHWEKLPELTFGADESIYEPFDWRDPFVFYNEEEECFDMLLAARLQGASEKNGGCVG 180 Query: 177 LCASKDLKSWEIRQPFWNPKMYVTHECPDLFQIGEWWYLVYSTFSERFVTHYRMSKSLSG 236 LC S DL W+ QPF+NP+ Y+THECPDLF++G+ WYL++STFSE+FVTHYRM+ +SG Sbjct: 181 LCRSYDLIHWKAMQPFYNPQSYMTHECPDLFKMGDKWYLIFSTFSEKFVTHYRMADKISG 240 Query: 237 PWTAPRNDAFDARAFYAAKTWSDGNKRYAFGWDPSKENENDYGDWQWAGNLVVHELVQEQ 296 PWTAP D FDARAFYAAKT + R AF W P+K ++D+G ++W GNL+ HE+ Q++ Sbjct: 241 PWTAPIEDTFDARAFYAAKTAQAKDVRMAFAWVPTKRGDSDFGQYEWGGNLIAHEIKQDK 300 Query: 297 DGTLTVRIPETVSHHFSNKQAAKWDSRIGEWEESEGRIETAANETYSCISAGFMPETCKI 356 DG L V+ + + F + A + +E NE M ETC I Sbjct: 301 DGKLKVKPSNGLINIFEKEITAN----------NIEDVEIKNNEGEKVYVIEGMKETCLI 350 Query: 357 SAELSFSEGTRGCGILLRASEDLDEAYYIRLEPIHNRLVFDMWPRREQGEMQWHIKGDHP 416 A + F EG+R GI LR +D YY+R EP +NR+V DMWPRR G QW++ GD P Sbjct: 351 EATIEFYEGSRAFGIGLRQDKDFANGYYLRFEPFYNRIVADMWPRRISGVNQWYVDGDKP 410 Query: 417 HAVELEVPIELQA--GTVYNIEIVVENSVCVVYLADQVAMTTRLYNLKTGSWGCFVQEG 473 VELE P + +A NI++ VE S+ +Y+ D VA+T R Y+ + WG FV++G Sbjct: 411 FMVELERPFDYKALEENKVNIKVFVEGSIICLYVNDTVALTMRAYSTERKYWGFFVKDG 469 >A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 protein Length = 478 Score = 425 bits (1092), Expect = e-117, Method: Compositional matrix adjust. Identities = 224/483 (46%), Positives = 293/483 (60%), Gaps = 17/483 (3%) Query: 3 NLFYKPDGAWVGDLIPFYKDGSFRLFYLHDWRDNKKIHGEGTSWFELHTSDFVHYEEVGE 62 ++F++P WVGDLIPF KDG F LFYLH+ R + K GTSW + T D +E+ G Sbjct: 4 SVFFQPTHGWVGDLIPFEKDGEFWLFYLHEDRSDPK---PGTSWNLVTTKDLTQFEDHGV 60 Query: 63 KLKHGSVSEQDLNCYTGSII-EAHGEYHLFYTGLNPNPEFIEDGVPLQCVMHAVSTD-ME 120 L HGS S+ D N YTGS++ +A G +HLFYTG NP DG+P Q VMHA STD M Sbjct: 61 SLSHGSESDLDFNAYTGSVLADAAGVHHLFYTGQNPR-NVGPDGMPRQLVMHATSTDGMR 119 Query: 121 SWRKIPEDTFYS-DGIQYERHDWRDPFVFWNEEAGEYWMLLAARNKEGPSRRRGSIGLCA 179 +W+K PE TF + DG YE DWRDPFVFW++ ++ MLLAAR+ EGP RRRG I C Sbjct: 120 TWQKHPELTFGAPDG--YESGDWRDPFVFWDDSKNQWRMLLAARHSEGPERRRGVIAQCV 177 Query: 180 SKDLKSWEIRQPFWNPKMYVTHECPDLFQIGEWWYLVYSTFSERFVTHYRMSKSLSGPWT 239 S DL +W+ +PFW+P+ Y+THECPD+F G+WWY+VYS FSE F T YRM+KS GPWT Sbjct: 178 STDLMTWQHTEPFWDPRRYITHECPDVFSWGDWWYMVYSEFSESFTTRYRMAKSPDGPWT 237 Query: 240 APRNDAFDARAFYAAKTWSDGNKRYAFGWDPSKENENDYGDWQWAGNLVVHELVQEQDGT 299 P D+ D RAFYA+KT +R+ FGW SKE D G WQWAG + V E Q DGT Sbjct: 238 VPALDSIDGRAFYASKTAERDGRRFFFGWIASKEGNADQGAWQWAGTMSVLEARQNPDGT 297 Query: 300 LTVRIPETVSHHFSNKQAAKWDSRIGEWEESEGRIETAANETYSCISAGFMPETCKISAE 359 L + + F + + + R+ T + +S +P+ A Sbjct: 298 LGFSFADELVESFWDDVSVPLTGSL------PARLHVPDGYT-ALVSDEELPQQFYAKAV 350 Query: 360 LSFSEGTRGCGILLRASEDLDEAYYIRLEPIHNRLVFDMWPRREQGEMQWHIKGDHPHAV 419 L + T CG+LLR+S D D +Y +RLEP RLVFD WPR G+ QWH+ GD P V Sbjct: 351 LDIAPDTTECGLLLRSSHDGDHSYVLRLEPKRGRLVFDRWPRTITGDAQWHVSGDVPFDV 410 Query: 420 ELEVPIELQAGTVYNIEIVVENSVCVVYLADQVAMTTRLYNLKTGSWGCFVQEGRAVFEQ 479 ELE P +L G + +E++V+ +CV + QVA++TR+Y+L G G F EG A + Sbjct: 411 ELERPCDLTPGE-HTLEVIVDGDICVAVVDRQVALSTRIYDLTAGRIGLFAGEGSATVTE 469 Query: 480 ASI 482 + Sbjct: 470 LEV 472 >C4GDD6.1 C4GDD6_9FIRM Putative uncharacterized protein Length = 548 Score = 422 bits (1085), Expect = e-116, Method: Compositional matrix adjust. Identities = 220/499 (44%), Positives = 294/499 (58%), Gaps = 15/499 (3%) Query: 1 LTNLFYKPDGAWVGDLIPFYKDGSFRLFYLHDWRD--NKKIHGEGTSWFELHTSDFVHYE 58 ++ +FY+P+G WVGD IPF DG F +FYLHD R ++ +G TSW L T D VH+E Sbjct: 42 MSGVFYQPEGGWVGDTIPFAHDGKFYIFYLHDERKGHSEDEYGYRTSWNLLITEDGVHFE 101 Query: 59 EVGEKLKHGSVSEQDLNCYTGSIIEAH-GEYHLFYTGLNP-NPEFIEDGVPLQCVMHAVS 116 + G L G + D CYTGS+I G +HLFYT N NP + DG PLQ V HA+S Sbjct: 102 DKGIVLPVGGYEDADYACYTGSVIAGKDGRFHLFYTAQNNYNPIYHRDGRPLQFVAHAIS 161 Query: 117 TDMESWRKIPEDTFYSDGIQYERHDWRDPFVFWNEEAGEYWMLLAARNKEGPSRRRGSIG 176 D+ W K P F +D YE DWRDPFVF+N E Y MLLAAR K + G +G Sbjct: 162 EDLIHWEKDPSAIFGADESIYEPFDWRDPFVFYNGEEDCYNMLLAARLKGAGDKNGGCVG 221 Query: 177 LCASKDLKSWEIRQPFWNPKMYVTHECPDLFQIGEWWYLVYSTFSERFVTHYRMSKSLSG 236 LC SKDL W+ +PF++P+ Y+THECPDLF++G WYLVYSTFSE+FVTHYR++ SL G Sbjct: 222 LCHSKDLIHWQAAEPFYHPESYMTHECPDLFRMGGKWYLVYSTFSEKFVTHYRVADSLGG 281 Query: 237 PWTAPRNDAFDARAFYAAKTWSDGNKRYAFGWDPSKENENDYGDWQWAGNLVVHELVQEQ 296 PWTAP D FD RAFYAAKT +R+AF W P++ +D+G ++W G L++H+L QE Sbjct: 282 PWTAPVEDTFDGRAFYAAKTAETKGRRWAFAWVPTRRGGSDFGQYEWGGTLLMHQLKQEA 341 Query: 297 DGTLTVRIPETVSHHFSNK---------QAAKWDSRIGEWEESEGRIETAANETYSCISA 347 DG L+V P V F + + K GE+ ++E + + Sbjct: 342 DGRLSVSPPRAVREAFEARLILCGPEPEEGGKETEDKGEYRRGGSKLELRNIQGKKNLIF 401 Query: 348 GFMPETCKISAELSFSEGTRGCGILLRASEDLDEAYYIRLEPIHNRLVFDMWPRREQGEM 407 M E C I A+L + EGTR G+ +R E +++ YY RLEP +NRLV DMWPRR G Sbjct: 402 DGMEEDCMIEADLIYEEGTRAFGLAVRQDEKMEKGYYFRLEPFYNRLVADMWPRRIAGVN 461 Query: 408 QWHIKGDHPHAVELEVPIELQA--GTVYNIEIVVENSVCVVYLADQVAMTTRLYNLKTGS 465 QW+I GD VELE P++ ++ ++ E S+ +Y+ D +A+T R Y Sbjct: 462 QWYIDGDKAFLVELERPLDFSKLQNRRLHLCLIAEGSILCLYVNDSLALTMRAYESGRSH 521 Query: 466 WGCFVQEGRAVFEQASISV 484 WG FV +G E ++ Sbjct: 522 WGFFVDDGGIQIENVRLTA 540 >C4ZAM5.1 C4ZAM5_9FIRM Putative beta-fructofuranosidase protein Length = 484 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 203/489 (41%), Positives = 288/489 (58%), Gaps = 34/489 (6%) Query: 1 LTNLFYKPDGAWVGDLIPFYKDGSFRLFYLHDWRDNKKIHGEGTSWFELHTSDFVHYEEV 60 + LFYKP+ AWVGDLIP+Y++G++ FYLHD R K + E T+W + T DFV+ + Sbjct: 1 MGRLFYKPENAWVGDLIPYYENGTYYGFYLHDPRIRDKEYAEDTTWHLIETKDFVNLDYK 60 Query: 61 GEKLKHGSVSEQDLNCYTGSII--EAHGEYHLFYTGLNPNPEFIEDGVPLQCVMHAVSTD 118 GE + G + + + N YTGS+I E G YH+FYT N + F G +Q VM A + Sbjct: 61 GESIARGGIHDANKNAYTGSVIKCEKDGLYHVFYTAYNED--FKLRGKTIQSVMQATGKN 118 Query: 119 MESWRKIPEDTFYSDGIQYERHDWRDPFVFWNEEAGEYWMLLAARNKEGPSRRRGSIGLC 178 +E+ + + F SDG QYE DWRDPFVFWN+E Y+MLLA+R R G I LC Sbjct: 119 LENLETVKDFLFVSDGKQYEEFDWRDPFVFWNDEDQCYYMLLASRVTNRGDLRGGCIALC 178 Query: 179 ASKDLKSWEIRQPFWNPKMYVTHECPDLFQIGEWWYLVYSTFSERFVTHYRMSKSLSGPW 238 S+DL +W +PF++PKMY+T ECP+LF++GE+WYLV+STFS++F THYR+SK+ +GPW Sbjct: 179 KSEDLINWTYEKPFYDPKMYITMECPELFKMGEYWYLVFSTFSDQFTTHYRISKNWNGPW 238 Query: 239 TAPRNDAFDARAFYAAKTWSDGNKRYAFGWDPSKENENDYGDWQWAGNLVVHELVQE-QD 297 P +D FD RA YA KT SDG +R+AFGW SK D+G W+W G + HE++Q ++ Sbjct: 239 EIPDDDVFDTRADYAIKTASDGKRRFAFGWIASKYGNTDFGPWEWGGTMNFHEIIQNSKN 298 Query: 298 GTLTVRIPETVSHHFSNKQAAKWDSRIGEWEESEGRIETAANETYSCISAGFMPETCKIS 357 G L ++ V +FS +E E + ET N G E+ + Sbjct: 299 GELRIKAIPEVLDYFS--------------KEIELKKETLYNCKMVQAQDGVSVESDTLG 344 Query: 358 AELSFSEGTRGC-------------GILLRASEDLDEAYYIRLEPIHNRLVFDMWPRREQ 404 A L EG GI L ++L+ Y++R+ P + + +DMWPR EQ Sbjct: 345 ALLYPLEGDTFALDMVIKPERYHEFGIALHVDKELETGYFLRMNPKNQLVAWDMWPRGEQ 404 Query: 405 GEMQWHIKGDHPHAVELEVPIELQAGTVYNIEIVVENSVCVVYLADQVAMTTRLYNLKTG 464 G QW IKGD P+ ++E +L Y + I+ E +CVVY+ D++ ++TR+YN K G Sbjct: 405 GRYQWQIKGDVPY--QIETARKLPKAEEYRVHIIKEKDICVVYINDEIVLSTRMYNHKDG 462 Query: 465 SWGCFVQEG 473 G +V +G Sbjct: 463 YAGIYVVQG 471 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,287,253,861 Number of extensions: 146303702 Number of successful extensions: 268122 Number of sequences better than 1.0e-05: 190 Number of HSP's gapped: 267911 Number of HSP's successfully gapped: 190 Length of query: 486 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 346 Effective length of database: 1,741,754,103 Effective search space: 602646919638 Effective search space used: 602646919638 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)