BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0011.p (57 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DC66.1 B1DC66_9BACL FAD linked oxidase domain protein 105 2e-21 B1HWA7.1 B1HWA7_LYSSC Reticuline oxidase EC=1.21.3.3 79 2e-13 Q796Y5.4 YGAK_BACSU Uncharacterized FAD-linked oxidoreductase yg... 78 2e-13 C3HV21.1 C3HV21_BACTU FAD linked oxidase domain protein 77 5e-13 A7Z2P6.1 A7Z2P6_BACA2 YgaK 76 8e-13 >B1DC66.1 B1DC66_9BACL FAD linked oxidase domain protein Length = 459 Score = 105 bits (261), Expect = 2e-21, Method: Composition-based stats. Identities = 48/54 (88%), Positives = 49/54 (90%) Query: 4 KGDYVNFPDLQIKNWPKAYYGENFGRLKQVKRKYDPHNVFHFAQSIPVSKQVGK 57 KGDYVNFPDLQIKNWPKAYYG NFGRLK+VKRKYDP NVF FAQSIPV KQV K Sbjct: 406 KGDYVNFPDLQIKNWPKAYYGVNFGRLKRVKRKYDPQNVFRFAQSIPVGKQVRK 459 >B1HWA7.1 B1HWA7_LYSSC Reticuline oxidase EC=1.21.3.3 Length = 456 Score = 78.6 bits (192), Expect = 2e-13, Method: Composition-based stats. Identities = 34/46 (73%), Positives = 38/46 (82%) Query: 5 GDYVNFPDLQIKNWPKAYYGENFGRLKQVKRKYDPHNVFHFAQSIP 50 GDYVNFPD I++WP AYYG NF RL++VK KYDP NVFHF QSIP Sbjct: 405 GDYVNFPDRFIQDWPTAYYGRNFRRLREVKTKYDPFNVFHFPQSIP 450 >Q796Y5.4 YGAK_BACSU Uncharacterized FAD-linked oxidoreductase ygaK EC=1.-.-.- Length = 451 Score = 78.2 bits (191), Expect = 2e-13, Method: Composition-based stats. Identities = 32/49 (65%), Positives = 39/49 (79%) Query: 2 EFKGDYVNFPDLQIKNWPKAYYGENFGRLKQVKRKYDPHNVFHFAQSIP 50 E GDYVN+PD++I+NWP+ YYGEN RL++VK YDP NVF F QSIP Sbjct: 395 ETMGDYVNWPDIEIRNWPRTYYGENVERLRRVKTTYDPENVFRFEQSIP 443 >C3HV21.1 C3HV21_BACTU FAD linked oxidase domain protein Length = 422 Score = 77.0 bits (188), Expect = 5e-13, Method: Composition-based stats. Identities = 32/46 (69%), Positives = 37/46 (80%) Query: 5 GDYVNFPDLQIKNWPKAYYGENFGRLKQVKRKYDPHNVFHFAQSIP 50 GDYVN+PD+ IKNW +YYG NF RL++VK YDP NVFHF QSIP Sbjct: 373 GDYVNWPDIDIKNWETSYYGSNFHRLRKVKTMYDPCNVFHFQQSIP 418 >A7Z2P6.1 A7Z2P6_BACA2 YgaK Length = 455 Score = 76.3 bits (186), Expect = 8e-13, Method: Composition-based stats. Identities = 32/49 (65%), Positives = 38/49 (77%) Query: 2 EFKGDYVNFPDLQIKNWPKAYYGENFGRLKQVKRKYDPHNVFHFAQSIP 50 E GDYVN+PD +I+NW + YYGEN RL+QVK KYDP N+F F QSIP Sbjct: 399 ETMGDYVNWPDREIRNWLQTYYGENVHRLRQVKTKYDPENIFRFEQSIP 447 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 381,479,003 Number of extensions: 11858194 Number of successful extensions: 22063 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 22157 Number of HSP's successfully gapped: 121 Length of query: 57 Length of database: 3,060,696,203 Length adjustment: 30 Effective length of query: 27 Effective length of database: 2,778,065,753 Effective search space: 75007775331 Effective search space used: 75007775331 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)