BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0005.p (252 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBT6.1 B3KBT6_9BACI Short-chain dehydrogenase/reductase SDR 437 e-121 Q9L2E3.1 Q9L2E3_STRCO Putative dehydrogenase/reductase (Putative... 307 7e-82 Q01TL3.1 Q01TL3_SOLUE Short-chain dehydrogenase/reductase SDR 293 8e-78 B4UI19.1 B4UI19_ANASK Short-chain dehydrogenase/reductase SDR 229 2e-58 C0ZE25.1 C0ZE25_BREBN Putative oxidoreductase 212 2e-53 >B3KBT6.1 B3KBT6_9BACI Short-chain dehydrogenase/reductase SDR Length = 252 Score = 437 bits (1123), Expect = e-121, Method: Compositional matrix adjust. Identities = 217/239 (90%), Positives = 227/239 (94%) Query: 14 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGNNVIAFLSDAGSLKDIA 73 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLG++ IA LSDA SLKDIA Sbjct: 14 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGSHAIAVLSDAASLKDIA 73 Query: 74 ALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPLLST 133 ALADRV+ EFGT+DALFVNAGVTGFVPFE+ EE YDEI TINAKGPYFTVQKLAPLL + Sbjct: 74 ALADRVEAEFGTVDALFVNAGVTGFVPFEAMTEEKYDEILTINAKGPYFTVQKLAPLLGS 133 Query: 134 GSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVIDTGIL 193 GSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTR LARELLPRNIRVNAVSPGVIDTGI+ Sbjct: 134 GSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRGLARELLPRNIRVNAVSPGVIDTGIM 193 Query: 194 EKAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFDATYTTGAEFPVDGGGSQI 252 EK+MP+EAAEQTKAQM QQIPMLRLGDPVEVAK VAFLAF+ATYTTGAEFPVDGGGSQI Sbjct: 194 EKSMPEEAAEQTKAQMRQQIPMLRLGDPVEVAKAVAFLAFEATYTTGAEFPVDGGGSQI 252 >Q9L2E3.1 Q9L2E3_STRCO Putative dehydrogenase/reductase (Putative secreted protein) Length = 247 Score = 307 bits (786), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 154/238 (64%), Positives = 179/238 (75%) Query: 15 VITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGNNVIAFLSDAGSLKDIAA 74 VI GG SG+G A A+LLV GA V+ITGR+QATLDAARE+LG N +A D SL D+ A Sbjct: 10 VIAGGGSGMGFAMAELLVAGGARVVITGRSQATLDAARERLGENAVAVRGDVASLPDLDA 69 Query: 75 LADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPLLSTG 134 L DRVK E GT+DALFVNAG+ P EST EE YDE+F IN KG +FTVQKLAPLL+TG Sbjct: 70 LTDRVKAELGTLDALFVNAGIATLTPLESTTEEAYDELFDINVKGAWFTVQKLAPLLNTG 129 Query: 135 SGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVIDTGILE 194 +GVVLTTS+ NV+G+ S YAA KAALRSM RS +RELLPR IRVNAVSPG +DTG L+ Sbjct: 130 AGVVLTTSVANVIGMTDTSVYAAGKAALRSMARSFSRELLPRGIRVNAVSPGPVDTGALD 189 Query: 195 KAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFDATYTTGAEFPVDGGGSQI 252 AM E AE+ KA+ PM R G P E+A+ AFLAFDATYTTGAE VDGG +Q+ Sbjct: 190 AAMTAEEAERFKAERVADNPMRRFGTPGEIARAAAFLAFDATYTTGAELAVDGGATQL 247 >Q01TL3.1 Q01TL3_SOLUE Short-chain dehydrogenase/reductase SDR Length = 249 Score = 293 bits (751), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 149/240 (62%), Positives = 181/240 (75%), Gaps = 2/240 (0%) Query: 15 VITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGNNVIAFLSDAGSLKDIAA 74 +ITGG++G+GLATAKL ++EGA V++TGR AT+ AA+ +LG+N I SDA SL D+ A Sbjct: 10 LITGGTTGLGLATAKLFIEEGARVIVTGRNPATIKAAQAELGDNAIVVPSDATSLPDMDA 69 Query: 75 LADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPLLSTG 134 LA +VK FG ID LFVNAG FVPFE+ E YDE+ +NAKGPYF VQKLAPL+ G Sbjct: 70 LATKVKETFGKIDVLFVNAGYGHFVPFEAVTEAVYDEMLNLNAKGPYFIVQKLAPLMPEG 129 Query: 135 SGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVID-TGIL 193 S VV TTSI NV +P LSAY A+KAALR+MTRS+A ELLPR IRVNAV+PG I T ++ Sbjct: 130 SSVVFTTSIANVKAVPTLSAYGAAKAALRAMTRSIAAELLPRGIRVNAVTPGPIGATDVM 189 Query: 194 EK-AMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFDATYTTGAEFPVDGGGSQI 252 + MPKEA +Q QM + +PM RLG P E+AK VAFLA DAT+TTGAE PVDGG SQ+ Sbjct: 190 HRLGMPKEAQDQVYLQMAESVPMKRLGRPEEIAKAVAFLAIDATFTTGAELPVDGGWSQL 249 >B4UI19.1 B4UI19_ANASK Short-chain dehydrogenase/reductase SDR Length = 248 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 121/239 (50%), Positives = 155/239 (64%), Gaps = 1/239 (0%) Query: 15 VITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGNNVIAFLSDAGSLKDIAA 74 V+TGG++GIGLA A++L++ GA VL+TG ++ L+ AR +LG SD L D+AA Sbjct: 10 VVTGGTAGIGLAIARVLLENGAEVLVTGSSEQGLEVARRELGPRARVVRSDTSKLADVAA 69 Query: 75 LADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPLLSTG 134 L V+ E G I A FVNAG + PF+ EE YD F +N KG YFT Q+LAPL+ G Sbjct: 70 LGAIVERELGRIHAAFVNAGFSKLTPFDRVTEEEYDRTFAVNTKGAYFTAQRLAPLVHEG 129 Query: 135 SGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVIDTGILE 194 V TTS+ + +G+P LSAY+ +KAA+RS R+LA ELLPR IRVNAVSPG T L Sbjct: 130 GAFVFTTSVADELGVPSLSAYSGAKAAVRSFVRTLATELLPRGIRVNAVSPGFTATPTLG 189 Query: 195 -KAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFDATYTTGAEFPVDGGGSQI 252 KE + + + PM R+G P EVA+ FLAFDAT+TTGAE PVDGG SQ+ Sbjct: 190 VTGASKEEVAAFEKEGNELTPMGRIGSPGEVARAALFLAFDATFTTGAELPVDGGLSQL 248 >C0ZE25.1 C0ZE25_BREBN Putative oxidoreductase Length = 253 Score = 212 bits (540), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 114/238 (47%), Positives = 150/238 (63%), Gaps = 1/238 (0%) Query: 15 VITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDAAREQLGNNVIAFLSDAGSLKDIAA 74 V+TGG+ G+GLA AK L+ GA V+++GR + A+ +LG +SD S+KD+ A Sbjct: 11 VVTGGTHGMGLAVAKALLAGGAEVIVSGRNPKNAEEAKRELGERAHVVVSDVSSMKDVQA 70 Query: 75 LADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPLLSTG 134 + V+ FG ID L VNAG + PF EETYD IF +N KG +FTVQ+LAPL+ G Sbjct: 71 FGEYVEKHFGKIDFLHVNAGTSILEPFLEVNEETYDRIFEVNTKGAFFTVQRLAPLIHEG 130 Query: 135 SGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVIDTGILE 194 +V T+S+ + G P +S Y+ASKAALRS+ S A ELL + IRVN VSPG IDT + Sbjct: 131 GSIVFTSSVADEGGYPGMSVYSASKAALRSLASSFAVELLDKGIRVNVVSPGFIDTPSMG 190 Query: 195 KAMPKEAAEQTKAQMTQQI-PMLRLGDPVEVAKVVAFLAFDATYTTGAEFPVDGGGSQ 251 A +A T ++ ++ PM R G EVAK V FLAFDAT+TTG+ VDGG Q Sbjct: 191 VAGFTDAERVTFQELGDKVTPMKRHGSSEEVAKAVLFLAFDATFTTGSRLAVDGGIGQ 248 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,271,096,280 Number of extensions: 47238015 Number of successful extensions: 291114 Number of sequences better than 1.0e-05: 31785 Number of HSP's gapped: 239577 Number of HSP's successfully gapped: 31863 Length of query: 252 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 120 Effective length of database: 1,817,122,223 Effective search space: 218054666760 Effective search space used: 218054666760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)