BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0004.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBT8.1 B3KBT8_9BACI Short-chain dehydrogenase/reductase SDR 513 e-144 B1D6N6.1 B1D6N6_9BACL Short-chain dehydrogenase/reductase SDR 432 e-119 B3K8G2.1 B3K8G2_9BACI Short-chain dehydrogenase/reductase SDR 278 4e-73 Q5WC36.1 Q5WC36_BACSK Putative uncharacterized protein 265 6e-69 A9B027.1 A9B027_HERA2 Short-chain dehydrogenase/reductase SDR 245 4e-63 >B3KBT8.1 B3KBT8_9BACI Short-chain dehydrogenase/reductase SDR Length = 289 Score = 513 bits (1321), Expect = e-144, Method: Compositional matrix adjust. Identities = 248/283 (87%), Positives = 265/283 (93%) Query: 14 LNTNQREHIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRA 73 ++ NQREHIALITGAS GIGLAL RK+LS+NWQV+ LNRSDFPADD +QN LK+G LRA Sbjct: 1 MSKNQREHIALITGASNGIGLALTRKMLSENWQVVALNRSDFPADDRFLQNHLKDGRLRA 60 Query: 74 YKVKDLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYI 133 YKVKDLTDYGSLR SLEEIKSKEQRIDILFNNAGGGLSELRYSKQGRE+HYELLTVVP+I Sbjct: 61 YKVKDLTDYGSLRHSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGRELHYELLTVVPFI 120 Query: 134 ILMELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWT 193 ILMELKELLKNGSLKTVINTSSQV RFTKEF+IE LEHPKTFRKMYGPYATSKLALSLWT Sbjct: 121 ILMELKELLKNGSLKTVINTSSQVFRFTKEFTIEKLEHPKTFRKMYGPYATSKLALSLWT 180 Query: 194 QAVAPQLAKEGIKIRSVDPGINNTLRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGAL 253 QAVAPQLAKEGIKIRSVDPGINNTLRKGKDSGLTA FELFMK FSSPPTHGANLL+EGAL Sbjct: 181 QAVAPQLAKEGIKIRSVDPGINNTLRKGKDSGLTAGFELFMKFFSSPPTHGANLLYEGAL 240 Query: 254 GKHRNETGVFLLKDRVADLKFTEQVQRVLDRISDIYSREFLDG 296 GK+R ETGVFL KDR+ADLKFTE QRVL++ISDIYS E+L+G Sbjct: 241 GKNRKETGVFLFKDRIADLKFTEHAQRVLEKISDIYSLEYLEG 283 >B1D6N6.1 B1D6N6_9BACL Short-chain dehydrogenase/reductase SDR Length = 285 Score = 432 bits (1111), Expect = e-119, Method: Compositional matrix adjust. Identities = 210/281 (74%), Positives = 236/281 (83%) Query: 14 LNTNQREHIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRA 73 +NTN+RE+I L+TGAS GIGL L RKLLS++WQV+ LNRSDFPADD IQ A+ GWLR Sbjct: 1 MNTNKRENIVLVTGASAGIGLELTRKLLSEDWQVVALNRSDFPADDTGIQKAIMEGWLRV 60 Query: 74 YKVKDLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYI 133 YK DLTDY SLRR+LEEIK KEQRIDILFNNAGG EL +SKQGRE HYELLTVVPYI Sbjct: 61 YKTADLTDYASLRRTLEEIKGKEQRIDILFNNAGGSFPELSHSKQGREKHYELLTVVPYI 120 Query: 134 ILMELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWT 193 ILMELKEL+KNGSLKTVINTSS L + KEF+IE LEHPKTFRK+ GPYATSKLALSLWT Sbjct: 121 ILMELKELIKNGSLKTVINTSSSALNYVKEFNIEILEHPKTFRKLLGPYATSKLALSLWT 180 Query: 194 QAVAPQLAKEGIKIRSVDPGINNTLRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGAL 253 QA+APQLAKEGIKIRSVDPG NNTLRKGK SGL + MK+F SPPTHGAN L+EGAL Sbjct: 181 QAIAPQLAKEGIKIRSVDPGSNNTLRKGKKSGLPLVIKWLMKLFFSPPTHGANQLYEGAL 240 Query: 254 GKHRNETGVFLLKDRVADLKFTEQVQRVLDRISDIYSREFL 294 G+HRN+TGVFLLK + A+LKF + + VL+RI +IY EFL Sbjct: 241 GEHRNKTGVFLLKGQTAELKFNDHSRNVLERIHEIYEHEFL 281 >B3K8G2.1 B3K8G2_9BACI Short-chain dehydrogenase/reductase SDR Length = 282 Score = 278 bits (711), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 147/273 (53%), Positives = 185/273 (67%), Gaps = 2/273 (0%) Query: 21 HIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKVKDLT 80 ++ALITGAS GIGL L ++LLS+ WQV+ L RS FP DD IQ+AL+ LR YK DL+ Sbjct: 8 NVALITGASSGIGLELTKRLLSEGWQVLALIRSAFPTDDARIQDALRLRQLRTYKA-DLS 66 Query: 81 DYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILMELKE 140 D+ L+ SL++IK E ID+LFNNAG EL YS+QGREMH+E+ +VVPYIILMELKE Sbjct: 67 DFTDLQASLDQIKKYEGHIDLLFNNAGVSFGELSYSQQGREMHFEVNSVVPYIILMELKE 126 Query: 141 LLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVAPQL 200 LL GS KTVINTSS L + K+F L P F+K+ GPYA SKLALSLWTQ +AP + Sbjct: 127 LLGKGSFKTVINTSSNALLYLKQFDHRTLASPVGFKKLIGPYARSKLALSLWTQEIAPSM 186 Query: 201 AKEGIKIRSVDPGINNTLRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGALGKHRNET 260 +GI IRSV PG N T K++G+ + +F S P+ GA+ L+E ALG+ R T Sbjct: 187 QADGITIRSVCPGGNKTTMT-KNAGMPKFMIPIRNLFFSHPSEGASRLYEAALGRSRQST 245 Query: 261 GVFLLKDRVADLKFTEQVQRVLDRISDIYSREF 293 GVFL K + LKF+ Q +L I IY +EF Sbjct: 246 GVFLNKGKATPLKFSSQGAELLSTIELIYKQEF 278 >Q5WC36.1 Q5WC36_BACSK Putative uncharacterized protein Length = 278 Score = 265 bits (676), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 144/281 (51%), Positives = 192/281 (68%), Gaps = 11/281 (3%) Query: 19 REH--IALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKV 76 +EH + LITGA+ GIGL L + LL Q W+V L RSDFP + +I +ALK LR YK Sbjct: 2 KEHNKVVLITGANSGIGLELTKNLLLQGWEVAALIRSDFPNEQPIIIDALKKNHLRVYKA 61 Query: 77 KDLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILM 136 DL D+ SLR +L+EIK E +ID+LFNNAG +E ++SKQGRE+H+E+ T+VP+II M Sbjct: 62 -DLADFSSLRNALDEIKKSETKIDMLFNNAGVSFAEYKHSKQGRELHFEVNTLVPFIITM 120 Query: 137 ELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKT-FRKMYGPYATSKLALSLWTQA 195 ELKELL G KT+INTSS L F K+F + + +PKT F+K+ GPYA SKLALSLWT Sbjct: 121 ELKELLLKGQNKTIINTSSNSLLFLKQFDLNTIINPKTPFKKLIGPYANSKLALSLWTNG 180 Query: 196 VAPQLAKEGIKIRSVDPGINNT-LRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGALG 254 +AP+L+ EGIKIRSV PG N T L KGK G+ ++ L + +PP+ GA+ L++ A Sbjct: 181 IAPELSSEGIKIRSVCPGPNKTRLTKGK--GMPSYILLIRNLMFTPPSKGASRLYDAAFN 238 Query: 255 KHRNE-TGVFLLKDRVADLKFTEQVQRVLDRISDIYSREFL 294 NE TG+F+ K + +K + + VL+R+ DIY EFL Sbjct: 239 ---NEITGIFINKGKETAIKLSGHEEEVLNRLYDIYKNEFL 276 >A9B027.1 A9B027_HERA2 Short-chain dehydrogenase/reductase SDR Length = 280 Score = 245 bits (625), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 128/271 (47%), Positives = 178/271 (65%), Gaps = 2/271 (0%) Query: 23 ALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKVKDLTDY 82 ALITGA+ GIGL L ++LL + W VI L RS FP ++ ++Q A G LR+Y+ +++D+ Sbjct: 10 ALITGANSGIGLELTKRLLGEGWAVIGLIRSQFPTNEPILQAAQTAGKLRSYQA-EISDF 68 Query: 83 GSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILMELKELL 142 LR +L+ IK +E +D+LFNNAG +L +S QGRE+ +EL +VVPYII MELKELL Sbjct: 69 TQLRAALQTIKQQETSLDLLFNNAGRATEQLVFSPQGRELSFELQSVVPYIIAMELKELL 128 Query: 143 KNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVAPQLAK 202 G KT+INTSS VL ++ I LE P++F+K++GPYATSKLALSLW QA+APQL K Sbjct: 129 LRGQNKTIINTSSNVLLALRKLEIAQLERPQSFKKLFGPYATSKLALSLWNQALAPQLEK 188 Query: 203 EGIKIRSVDPGINNTLRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGALGKHRNETGV 262 EGIK+ SV PG N K ++GL W F + P++GA L+ A + + +G Sbjct: 189 EGIKLYSVCPGGNRGSLK-TNNGLPWWLRPFHGLLFKHPSNGAERLYNTAFNQQQFPSGS 247 Query: 263 FLLKDRVADLKFTEQVQRVLDRISDIYSREF 293 F+ K + L +Q VL+++ IY +EF Sbjct: 248 FINKGKPTALPAAQQAHAVLNKVQAIYQQEF 278 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,728,591,635 Number of extensions: 68929185 Number of successful extensions: 245658 Number of sequences better than 1.0e-05: 1622 Number of HSP's gapped: 250422 Number of HSP's successfully gapped: 1624 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)