BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0024.p (469 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KI00.1 B3KI00_9BACI Type III effector Hrp-dependent outer protein 420 e-115 Q8ELG7.1 Q8ELG7_OCEIH Hypothetical conserved protein 369 e-100 Q5WAJ7.1 Q5WAJ7_BACSK Putative uncharacterized protein 349 3e-94 C1U7X8.1 C1U7X8_9SPHI Uncharacterized conserved protein 335 4e-90 B1G889.1 B1G889_9BURK Type III effector Hrp-dependent protein 330 2e-88 >B3KI00.1 B3KI00_9BACI Type III effector Hrp-dependent outer protein Length = 468 Score = 420 bits (1080), Expect = e-115, Method: Compositional matrix adjust. Identities = 222/456 (48%), Positives = 298/456 (65%), Gaps = 12/456 (2%) Query: 6 LAFYGDDFTGSTDAMEMLATRGYRTVLFLEPPNPEELKRNYPDVDCIGIAGISRSLPSGK 65 L FYGDDFTGSTDAME LA G TVLFL+PP PE L +P+V C G+AG+SRS+ + Sbjct: 15 LCFYGDDFTGSTDAMEALAKSGADTVLFLDPPPPELLAERFPNVRCFGVAGVSRSMTPDE 74 Query: 66 LEEELAPIFEQLLAYQPSVIHYKVCSTFDSAPEIGSIGRVIDIGKRMFSNQQVIPVLAGA 125 +E EL P E+L AY +V+HYKVCSTFDS+P GSIG+V+++G+ ++ Q+ +P+L G Sbjct: 75 MERELRPALERLHAYGTAVVHYKVCSTFDSSPRAGSIGKVLELGRSVYPRQRYVPILVGV 134 Query: 126 PPLSRYTVFGQHFAAAG--GTIHRLDRHPIMSVHPATPMDEADLRLHLGRQTELPVGLMS 183 P L RYT+FG HFAAAG G +RLDRHP MS HP TPM E+DLRLHL QT+LPV LM Sbjct: 135 PALKRYTLFGHHFAAAGADGGTYRLDRHPTMSRHPITPMRESDLRLHLREQTDLPVSLME 194 Query: 184 VLELEGDEEEVWQTFSGK----EEPVVLFDSLGRRDTLASGKLLWVMAQAADTPLFVVGS 239 L+G EEV + E +LFD + A+G+L+W A+ T LFV+GS Sbjct: 195 WTALDGVPEEVSARLEHRLEVGEPAALLFDVIDDPRLEAAGRLMWREAEERGTGLFVIGS 254 Query: 240 SGVEFALAEVWQSTSGLKRELPDYTANHV-IAEAAEPIVVLSGSASDTTRRQIEVAEQEG 298 SGVE+ALA W K L D + AE A+ ++V+SGS S T+ QI A + G Sbjct: 255 SGVEYALAAHWA-----KEGLVDPGGARLQAAEQADQLLVVSGSCSPVTQSQIAWALKRG 309 Query: 299 FISVRLPLPEFLNPDTAEAAMEKLLGSADEALQNGCDIIFYTAKGPKDPAISAMKEKLAA 358 + +R+ ++L P A+ A LL A ++ G +II Y+A GP+DPAI+A++E L A Sbjct: 310 YAGIRVRARDWLEPGRADEARRSLLKQALPFVEQGRNIIVYSADGPEDPAIAALREALNA 369 Query: 359 AGILPDMSGQLLGQLLGRIMKEIIMRSRTRRFVVAGGDTSGFATKELGISAMEIAVPVAP 418 G+ P SG+L+G LGR+ +++ + RR ++AGGDTSG+ T+ELGI A+ + P Sbjct: 370 RGMEPADSGRLIGTQLGRLTSDLLRATGLRRILIAGGDTSGYVTRELGIYALSCLSAIVP 429 Query: 419 GAPLNLCYSSDSLVNGLELALKGGQLGQEDYFLRVK 454 G PL CYS D ++GL+LALKGGQ+G DYF RV+ Sbjct: 430 GGPLCRCYSEDPRLDGLQLALKGGQVGGADYFERVR 465 >Q8ELG7.1 Q8ELG7_OCEIH Hypothetical conserved protein Length = 446 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 194/453 (42%), Positives = 279/453 (61%), Gaps = 13/453 (2%) Query: 1 MKHLRLAFYGDDFTGSTDAMEMLATRGYRTVLFLEPPNPEELKRNYPDVDCIGIAGISRS 60 MK L L FYGDDFTGSTDAME L G +T+LFL+ P+ E + R + VDCIG+AG +R+ Sbjct: 1 MKRLLLGFYGDDFTGSTDAMEALDQYGLKTILFLKIPDQEMIDR-FRHVDCIGVAGTARA 59 Query: 61 LPSGKLEEELAPIFEQLLAYQPSVIHYKVCSTFDSAPEIGSIGRVIDIGKRMFSNQQVIP 120 + +EEEL+P++E L P+ +HYKVCSTFDSAPE+GSIG IDI + F+ P Sbjct: 60 KGTKGMEEELSPVYEFLRQLNPTFVHYKVCSTFDSAPEVGSIGFAIDIARNYFTKGSY-P 118 Query: 121 VLAGAPPLSRYTVFGQHFAAAGGTIHRLDRHPIMSVHPATPMDEADLRLHLGRQTELPVG 180 +L AP L RYTVFG+HFA I+RLD+HP+MS HP TPMDEADL HL QT+ + Sbjct: 119 LLVAAPNLGRYTVFGEHFAKFQEEIYRLDKHPVMSKHPVTPMDEADLAKHLQAQTKQKIA 178 Query: 181 LMSVLELEGDEEEVWQTFSGKEEPVVLFDSLGRRDTLASGKLLWVMAQAADTPLFVVGSS 240 ++VL+LE + + E ++L+D+L ++LW + +D P F+VGSS Sbjct: 179 KVNVLDLEANTAQQIINQENNEADIILYDALNASHMKKVSEVLW--EKRSDEPQFIVGSS 236 Query: 241 GVEFALAEVWQSTSGLKRELPDYTANHVIAEAAEPIVVLSGSASDTTRRQIEVAEQEGFI 300 GVE+A+ + + + +E + ++V + +SGS SD T++Q+E AE+ GF Sbjct: 237 GVEYAIGDAVRREGVIAKENKATSPSNV-----HQLFAVSGSVSDVTKQQLENAEEAGFE 291 Query: 301 SVRLPLPEFLNPDTAEAAMEKLLGSADEALQNGCDIIFYTAKGPKDPAISAMKEKLAAAG 360 +++P + +P EA ++ ++ +E + +I YTAKG +DP I+ KE L G Sbjct: 292 LIQIPFEMYTDPSLVEAFLDNIIQQIEEKKK----VIMYTAKGNEDPIIARTKEHLLNNG 347 Query: 361 ILPDMSGQLLGQLLGRIMKEIIMRSRTRRFVVAGGDTSGFATKELGISAMEIAVPVAPGA 420 I G +G+ LG K +I R+ RR +++GGDTSGF T L I +E+ +APGA Sbjct: 348 INKQDIGIYIGEKLGEWTKSVIDRADLRRVIISGGDTSGFVTSNLDIYGLEVLDSIAPGA 407 Query: 421 PLNLCYSSDSLVNGLELALKGGQLGQEDYFLRV 453 PL + YS + +GLE+ALK GQLG +F +V Sbjct: 408 PLCIAYSERAKYDGLEIALKSGQLGGPAFFEKV 440 >Q5WAJ7.1 Q5WAJ7_BACSK Putative uncharacterized protein Length = 445 Score = 349 bits (896), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 196/457 (42%), Positives = 266/457 (58%), Gaps = 18/457 (3%) Query: 1 MKHLRLAFYGDDFTGSTDAMEMLATRGYRTVLFLEPPNPEELKRNYPDVDCIGIAGISRS 60 M L L FYGDDFTGSTDAME L G +T+LFL P+ LKR + VDCIG+AG +R+ Sbjct: 1 MNRLLLGFYGDDFTGSTDAMEALDQYGLKTILFLRIPDQGMLKR-FEAVDCIGVAGTARA 59 Query: 61 LPSGKLEEELAPIFEQLLAYQPSVIHYKVCSTFDSAPEIGSIGRVIDIGKRMFSNQQVIP 120 ++EEL P+F L P +HYKVCSTFDS+PE GSIG D+ +R F P Sbjct: 60 KGKAGMKEELEPVFSFLRDLHPFFVHYKVCSTFDSSPETGSIGFASDLARRYFEKGSY-P 118 Query: 121 VLAGAPPLSRYTVFGQHFAAAGGTIHRLDRHPIMSVHPATPMDEADLRLHLGRQTELPVG 180 +L AP L RYTVFG HFAA + RLD HP+M+ HP TPMDEADL HL +QT+ + Sbjct: 119 LLVAAPNLGRYTVFGHHFAAFNNQVFRLDTHPVMARHPVTPMDEADLAKHLQKQTKQKIA 178 Query: 181 LMSVLELEGDEEEVWQTFSGKEEP-VVLFDSLGRRDTLASGKLLWVMAQAADTPLFVVGS 239 +++L+LE + + G+ E ++++D+L R ++LW + + FVVGS Sbjct: 179 NVTILDLEA--KSASEIVKGESEAGLIVYDALNRGHMEKVSEVLW--EKRGERAQFVVGS 234 Query: 240 SGVEFALAEVWQSTSGLKRELPDYTANHVIAEA-AEPIVVLSGSASDTTRRQIEVAEQEG 298 SGVE+AL E +KRE + + + AE I +SGS SD T+ Q+ AE G Sbjct: 235 SGVEYALGE------AMKREKQTQRTDRLKKPSQAERIFAVSGSVSDVTKSQLLAAEAAG 288 Query: 299 FISVRLPLPEFLNPDTAEAAMEKLLGSADEALQNGCDIIFYTAKGPKDPAISAMKEKLAA 358 F ++++P+ F T EA E L + ++ YTAKG DP I ++ L+ Sbjct: 289 FHAIQIPVELF----TDEAKAEAFLADVAAMIAAKQKVVIYTAKGADDPVIVETRKHLSE 344 Query: 359 AGILPDMSGQLLGQLLGRIMKEIIMRSRTRRFVVAGGDTSGFATKELGISAMEIAVPVAP 418 +G+ D G +G+ LG K I+ R RR +++GGDTSGF T L I +E+ VAP Sbjct: 345 SGVAKDEIGIFIGEKLGAWTKSILDRVDVRRVIISGGDTSGFVTSNLDIYGLEVLDSVAP 404 Query: 419 GAPLNLCYSSDSLVNGLELALKGGQLGQEDYFLRVKK 455 GAPL L YS +GLE+ALK GQLG E +F V + Sbjct: 405 GAPLCLAYSESEKFDGLEIALKSGQLGGEHFFENVYR 441 >C1U7X8.1 C1U7X8_9SPHI Uncharacterized conserved protein Length = 461 Score = 335 bits (860), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 196/462 (42%), Positives = 269/462 (58%), Gaps = 25/462 (5%) Query: 2 KHLRLAFYGDDFTGSTDAMEMLATRGYRTVLFLEPPNPEELKRNYPDVDCIGIAGISRSL 61 K+L LAFYGDDFTGSTD +E L G RT LF+ PP PE+L R +PD+ GIAG++R++ Sbjct: 4 KNLLLAFYGDDFTGSTDVLETLERAGIRTALFMAPPTPEDLVR-FPDIRAFGIAGMTRAM 62 Query: 62 PSGKLEEELAPIFEQLLAYQPSVIHYKVCSTFDSAPEIGSIGRVIDIGKRMFSNQQVIPV 121 ++E+ L P F L IHYKVCSTFDS+P IGSIG+ I++G MF N + + + Sbjct: 63 TPPQMEKTLMPAFAALKKSGARHIHYKVCSTFDSSPAIGSIGKAIEVGIEMF-NPRFVSL 121 Query: 122 LAGAPPLSRYTVFGQHFAAAG----GTIHRLDRHPIMSVHPATPMDEADLRLHLGRQTEL 177 + AP L RY FG FA G G IHRLDRHP MS HP TP DEADLRLHL +QT Sbjct: 122 IVAAPALGRYCAFGNLFARMGIGSNGHIHRLDRHPSMSKHPTTPADEADLRLHLSKQTSK 181 Query: 178 PVGLMSVLELEGDEEEVWQTFSG--KEEPVVLFDSLGRRDTLASGKLLWVMAQAADTPLF 235 GL+ +L++EG+ E +E +VLFD+L L G+L+ + + P F Sbjct: 182 TCGLLDILQIEGEPREAAAALENTVRENDIVLFDALYPHQMLRIGELIDPFG-SPNAPQF 240 Query: 236 VVGSSGVEFALAEVWQSTSGLKRELPDYTANHVIAEAAEPIVVLSGSASDTTRRQIEVAE 295 VGSSGVE AL W + +G+ R ++ ++ P++V+SGS S T RQI A Sbjct: 241 SVGSSGVEMALTACWNN-AGIARNRTEWPEPGEVS----PLLVVSGSCSPVTSRQITYAL 295 Query: 296 QEGFISVRLPLPEF---LNPDTAEAAMEKLLGSADEALQNGCDIIFYTAKGPKDPAISA- 351 GF + L P ++ T +K++ E L+ G +I +T+K KD +A Sbjct: 296 ARGFEEIALDTPAIATGMHDPTFADDTQKVV----EFLRRGRSVIIHTSKSTKDERFTAS 351 Query: 352 ---MKEKLAAAGILPDMSGQLLGQLLGRIMKEIIMRSRTRRFVVAGGDTSGFATKELGIS 408 +K K +A + + L G+ LGRI + + RR V+AGGDTSG K LGI+ Sbjct: 352 NNILKNKGLSATEIQTRTSSLYGEALGRIARAAMASISVRRLVIAGGDTSGAVAKTLGIN 411 Query: 409 AMEIAVPVAPGAPLNLCYSSDSLVNGLELALKGGQLGQEDYF 450 A+ + P++PGAPL ++ S +GLE+ KGGQ+G EDYF Sbjct: 412 ALSMIAPLSPGAPLCRAHAPGSPADGLEINFKGGQVGGEDYF 453 >B1G889.1 B1G889_9BURK Type III effector Hrp-dependent protein Length = 470 Score = 330 bits (847), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 189/453 (41%), Positives = 264/453 (58%), Gaps = 11/453 (2%) Query: 4 LRLAFYGDDFTGSTDAMEMLATRGYRTVLFLEPPNPEELKRNYPDVDCIGIAGISRSLPS 63 L LA+YGDDFTGSTDAME + G TVL L+ P PE L R +P+V C+G+AG SR S Sbjct: 22 LLLAYYGDDFTGSTDAMEAMQAAGVPTVLCLQKPTPELLAR-FPEVRCVGMAGSSRGRSS 80 Query: 64 GKLEEELAPIFEQLLAYQPSVIHYKVCSTFDSAPEIGSIGRVIDIGKRMFSNQQVIPVLA 123 +++EL + L A ++ YKVCSTFDS+PE+GSIGR IDIG R P++ Sbjct: 81 AWMDDELPDVLASLAALGAPILQYKVCSTFDSSPEVGSIGRAIDIGVRHMPGNWS-PMVI 139 Query: 124 GAPPLSRYTVFGQHFAAAGGTIHRLDRHPIMSVHPATPMDEADLRLHLGRQTELPVGLMS 183 GAP L RY +FG FAA G +RLDRHP MS HP TPM+EADLRLHL RQT + L+ Sbjct: 140 GAPRLKRYQMFGNLFAAVDGVGYRLDRHPTMSRHPVTPMNEADLRLHLARQTARRIELID 199 Query: 184 VLELEG-DEEEVWQTFSGKEEPVVLFDSLGRRDTLASGKLLWVMAQAADTPLFVVGSSGV 242 +LEL G D + + PVVL D L +G+L+W + +F SSG+ Sbjct: 200 MLELRGADVATRVRALCAPDMPVVLIDVLDEETLAEAGRLVW---EQRGEGIFTASSSGL 256 Query: 243 EFALAEVWQSTSGLKRELPDYTANHVIAEAAEPIVVLSGSASDTTRRQIEVAEQEGFISV 302 ++ALA W++ GL P A A+ + I +SGS S T QI A GF + Sbjct: 257 QYALAAHWRA-RGLLPPTPSLPA----ADPVQAIAAVSGSCSPVTAAQIGWARAHGFHTE 311 Query: 303 RLPLPEFLNPDTAEAAMEKLLGSADEALQNGCDIIFYTAKGPKDPAISAMKEKLAAAGIL 362 RL LP L+ A +E+++ +A +AL G +I ++A+GP DPA++ +AAG Sbjct: 312 RLDLPRALDSRDGAAEIERVVTAATQALTRGISVIVHSAEGPDDPAVTGFDAIASAAGFA 371 Query: 363 PDMSGQLLGQLLGRIMKEIIMRSRTRRFVVAGGDTSGFATKELGISAMEIAVPVAPGAPL 422 + + +G+ L +M+ ++ R VVAGGD+SG LGI A+ + + PGAPL Sbjct: 372 RHDAARKVGRALAEVMRRLLDSVELTRVVVAGGDSSGEVASVLGIDALSVMAGLVPGAPL 431 Query: 423 NLCYSSDSLVNGLELALKGGQLGQEDYFLRVKK 455 +S++ +GL++ LKGGQ+G +F V++ Sbjct: 432 CRAWSAEPRRDGLQIVLKGGQIGDATFFGMVRE 464 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,907,750,396 Number of extensions: 126657025 Number of successful extensions: 310157 Number of sequences better than 1.0e-05: 355 Number of HSP's gapped: 308872 Number of HSP's successfully gapped: 356 Length of query: 469 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 330 Effective length of database: 1,751,175,118 Effective search space: 577887788940 Effective search space used: 577887788940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)