BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0022.p (342 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q7UTN8.1 Q7UTN8_RHOBA Probable NADH-dependent dyhydrogenase 258 8e-67 Q2CF05.1 Q2CF05_9RHOB Probable NADH-dependent dyhydrogenase 216 3e-54 A8TX75.1 A8TX75_9PROT Probable NADH-dependent dyhydrogenase 213 2e-53 A4ELP4.1 A4ELP4_9RHOB Probable NADH-dependent dyhydrogenase 206 3e-51 A0P1T4.1 A0P1T4_9RHOB Probable NADH-dependent dyhydrogenase 205 7e-51 >Q7UTN8.1 Q7UTN8_RHOBA Probable NADH-dependent dyhydrogenase Length = 349 Score = 258 bits (658), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 134/334 (40%), Positives = 208/334 (62%), Gaps = 8/334 (2%) Query: 5 QGIIVGSGHFAHIQLEAWAEVQGGGIIAVVSRDVEGARKLAEAYQIQSWGADTEIHQLID 64 +G ++G+G+F+ +AW + I++ D+E A +A+ + ++S +D Q++D Sbjct: 11 RGAVIGAGYFSQFHYDAWKRMPDVEIVSCCDVDMEKANDVAQTHGVKSVYSD--YRQMLD 68 Query: 65 SLNPDFLDICTPPDSHLEYARIAADRGIAVLCQKPVAPTQEESEELVRYCRDKGVPVMIN 124 DF+DI T PD+HLE + RGIA++CQKP+APT +E+ E+V ++G+ M++ Sbjct: 69 QHQVDFVDIITRPDTHLELVDEISQRGIAIICQKPLAPTVQEAREMVSLVEERGIRFMVH 128 Query: 125 ENWRWQGWYREIRSIIDSGRLG-RLFQVYFAMRPGDGYGDSPY-PLQPYFKDMEKFLLYE 182 EN+R+Q WYRE++ +++ G +G RL + F R GDG+G Y QPYF+ MEKFL++E Sbjct: 129 ENFRFQPWYREMKRLMEDGVIGDRLHSLSFRNRAGDGHGPEAYLARQPYFQTMEKFLIFE 188 Query: 183 TGIHWIDTFRYLFGEIASVYCQLRTWNPLVKGEDAATVLFTFVSGMTGIYDANRTVYNAE 242 GIH IDTFRYL GEI +C R NP++ GED A +F F G G+YDANR + Sbjct: 189 AGIHTIDTFRYLGGEIEQTWCWHRKLNPVIAGEDTAVGVFRFEGGAMGLYDANRFNESTS 248 Query: 243 IRPP-AYGKMTLEAELGNLRLDEDGRIFITLRGGTEQEHIYT-IPDGWKGGSVISTHQHF 300 P +G++ LE + G++RL +D R+ I G TE++H Y+ G+ G V +T +HF Sbjct: 249 KNPRYTFGEVLLEGDGGSIRLYDDARLTIQSLGETERDHAYSPTQHGFSGDCVFATQRHF 308 Query: 301 IDGLHSGDSNYFETEGEKYMISQRIVYACYQSAK 334 ID L +G++ FET+G Y+ S + A Y S++ Sbjct: 309 IDNLATGET--FETDGPNYLKSLAVQEAMYASSE 340 >Q2CF05.1 Q2CF05_9RHOB Probable NADH-dependent dyhydrogenase Length = 347 Score = 216 bits (550), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 128/340 (37%), Positives = 184/340 (54%), Gaps = 11/340 (3%) Query: 9 VGSGHFAHIQLEAWAEVQGGGIIAVVSRDVEGARKLAEAYQIQSWGADTEIHQLIDSL-- 66 VG+G+FA Q+ AW ++ G + + D + AR +I G + +H + L Sbjct: 11 VGAGYFAQFQIAAWHDLPGVALAGLC--DTDTARAAQTLERIVGPGHEVPVHARAEELFA 68 Query: 67 -NPDFLDICTPPDSHLEYARIAAD-RGIAVLCQKPVAPTQEESEELVRYCRDKGVPVMIN 124 + +DI TPP +H A R ++CQKP TQE + GVP++++ Sbjct: 69 AGAEIIDIATPPATHAALIAAAIAARPRLIVCQKPFCGTQEAALAATEAAEAAGVPLVVH 128 Query: 125 ENWRWQGWYREIRSIIDSGRLGRLFQVYFAMRPGDGYGDSPY-PLQPYFKDMEKFLLYET 183 EN R+Q WYR I++ + +GR+G +FQ+ +RPGDG G Y QPYF+DME+FL++ET Sbjct: 129 ENVRFQPWYRRIKAELAAGRIGEVFQLTMRLRPGDGQGAQAYLDRQPYFRDMERFLVHET 188 Query: 184 GIHWIDTFRYLFGEIASVYCQLRTWNPLVKGEDAATVLFTFVSGMTGIYDANRTV-YNAE 242 GIHWIDTFRYL GE SV+ LR N + GEDA +LF F I+DANR+ + A+ Sbjct: 189 GIHWIDTFRYLLGEPRSVFADLRRTNSGIAGEDAGMLLFRFDGAARAIFDANRSADHAAD 248 Query: 243 IRPPAYGKMTLEAELGNLRLDEDGRIFITLRGGTEQEHIYT-IPDGWKGGSVISTHQHFI 301 R G+ T E G L LD GR+ + G E E + PDG+ G V + QH + Sbjct: 249 NRRHTMGEFTAEGAAGTLALDGYGRLTVRAFGANEVELLDAWAPDGFGAGCVRAFQQHVL 308 Query: 302 DGLHSGDSNYFETEGEKYMISQRIVYACYQSAKEQKAISI 341 DGL +G FET Y+ + I Y SA + +++ Sbjct: 309 DGLTTGAG--FETGARDYLRNLAIEEQVYASADAGRVVAL 346 >A8TX75.1 A8TX75_9PROT Probable NADH-dependent dyhydrogenase Length = 355 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 125/347 (36%), Positives = 187/347 (53%), Gaps = 13/347 (3%) Query: 4 LQGIIVGSGHFAHIQLEAW---AEVQGGGIIAVVSRDVEGARKLAEAYQIQSWGADTEIH 60 L+ + VG+G+FA ++ W +V G+ A V RD A+A D + Sbjct: 8 LKAVTVGAGYFAGFHVDGWLRNPDVTFAGL-ADVDRDKASGMLAAKAGADHGVAVDADAD 66 Query: 61 QLIDSLNPDFLDICTPPDSHLEYARIAADRGI-AVLCQKPVAPTQEESEELVRYCRDKGV 119 +L D++ PD +DI TPP +H + A + A++CQKP + +E+ E V + Sbjct: 67 RLFDAIRPDIIDIATPPPAHFALIKQALETDARAIICQKPFCTSLDEAREAVHLIEQRKR 126 Query: 120 PVMINENWRWQGWYREIRSIIDSGRLGRLFQVYFAMRPGDGYGDSPY-PLQPYFKDMEKF 178 V+++EN+R+Q WYR IR + SGR+G L+Q+ F MRPGDG G Y QPYF+ ME+F Sbjct: 127 LVVVHENFRFQPWYRAIREELSSGRVGDLYQISFRMRPGDGRGPDAYLSRQPYFQKMERF 186 Query: 179 LLYETGIHWIDTFRYLFGEIASVYCQLRTWNPLVKGEDAATVLFTFVSGMTGIYDANR-T 237 L++ET +HWIDTFRYL GE SV+ LR NP++ GEDA +LF + G ++D NR T Sbjct: 187 LIHETAVHWIDTFRYLMGEPDSVFADLRRLNPVIAGEDAGFLLFRYPDGRRAVFDGNRLT 246 Query: 238 VYNAEIRPPAYGKMTLEAELGNLRLDEDGRIFITLRGGTEQEHI---YTIPDGWKGGSVI 294 + A+ G+ +E G + LD G + G E + I Y+ G+ G SV Sbjct: 247 DHVADNTRFTMGECLIEGSDGQIALDGFGNLTFRSTGSREWQPIGGGYSQA-GFGGDSVF 305 Query: 295 STHQHFIDGLHSGDSNYFETEGEKYMISQRIVYACYQSAKEQKAISI 341 + +H D L +G E + Y+ + I YA Y SA + + + Sbjct: 306 ALQKHVSDHLLTGSP--IENDAASYLRNVEIEYALYDSATAGRVVDL 350 >A4ELP4.1 A4ELP4_9RHOB Probable NADH-dependent dyhydrogenase Length = 342 Score = 206 bits (524), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 114/340 (33%), Positives = 182/340 (53%), Gaps = 18/340 (5%) Query: 2 EQLQGIIVGSGHFAHIQLEAWAEVQGGGIIAVVSRDVEGARKLAEAYQIQSWGADTEIHQ 61 + L+ VG+G+FA ++WA + ++ RD E A+ A A T++ Q Sbjct: 5 QNLRIACVGAGYFAQFHYDSWARMARAILVGACDRDFERAKATGLA-------AYTDLAQ 57 Query: 62 LIDSLNPDFLDICTPPDSHLEYARIAADRGIA-VLCQKPVAPTQEESEELVRYCRDKGVP 120 ++D PD LD+ PP +H + RIA G+ ++CQKP E++ +V + Sbjct: 58 MLDEQAPDLLDVIVPPQAHADTIRIALAAGVKWIICQKPFCLNLTEAQTVVAEAKTANAT 117 Query: 121 VMINENWRWQGWYREIRSIIDSGRLGRLFQVYFAMRPGDGYGDSPY-PLQPYFKDMEKFL 179 ++++EN+R+Q WYR I++ +D+G +G+ QV F +RPGDG G Y QPYF+ M +FL Sbjct: 118 IVVHENFRFQPWYRTIKTALDTGTIGQAHQVTFRLRPGDGQGPDAYLDRQPYFQKMPRFL 177 Query: 180 LYETGIHWIDTFRYLFGEIASVYCQLRTWNPLVKGEDAATVLFTFVSGMTGIYDANRTV- 238 ++ETG+HW+DTFRYL G +VY LR NP + GEDA +LF +G+ ++D NR + Sbjct: 178 VHETGVHWVDTFRYLLGNPTAVYADLRQINPAIVGEDAGYILFDHPNGVRALFDGNRHLD 237 Query: 239 YNAEIRPPAYGKMTLEAELGNLRLDEDGRIFITLRGGTEQEHIYTI-PDGWK---GGSVI 294 + A+ G+ +E LG +RL DG +T R + + PD W G V Sbjct: 238 HKADNLRRTMGEALIEGTLGTIRLYGDGS--VTHRDFADATETTLLGPDTWDGFGGDCVH 295 Query: 295 STHQHFIDGLHSGDSNYFETEGEKYMISQRIVYACYQSAK 334 + H + G+ G E + Y+ +I Y++A+ Sbjct: 296 ALQSHIMRGVLDGAP--LENTADNYLTVMKIEEQIYEAAR 333 >A0P1T4.1 A0P1T4_9RHOB Probable NADH-dependent dyhydrogenase Length = 352 Score = 205 bits (521), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 116/343 (33%), Positives = 181/343 (52%), Gaps = 14/343 (4%) Query: 2 EQLQGIIVGSGHFAHIQLEAWAEVQGGGIIAVVSRDVEGARKLAEAYQIQSWGADTEIHQ 61 + L+ +G+G+FA +AW V+ ++ +D A+ A +++ Sbjct: 13 DTLRVACLGAGYFAQFHYDAWRRVERVRLVGSCDQDPAKAKA-------TGLTAFSDLEC 65 Query: 62 LIDSLNPDFLDICTPPDSHLEYARIAADRGI-AVLCQKPVAPTQEESEELVRYCRDKGVP 120 ++ PD LDI TPP +H +A +G+ ++CQKP + EE+ + + GV Sbjct: 66 MLKMAKPDLLDIITPPPTHFRAIELAIRKGVKTIICQKPFCTSLEEARKATKLAEGAGVT 125 Query: 121 VMINENWRWQGWYREIRSIIDSGRLGRLFQVYFAMRPGDGYGDSPY-PLQPYFKDMEKFL 179 ++I+EN+R+Q WYR IR ++ LG L QV F +R GDG G + Y QPYF++M + L Sbjct: 126 IVIHENFRFQPWYRTIRQALERQLLGDLLQVTFRLRTGDGQGPNAYLDRQPYFQEMPRLL 185 Query: 180 LYETGIHWIDTFRYLFGEIASVYCQLRTWNPLVKGEDAATVLFTFVSGMTGIYDANRTV- 238 ++ETG+HWIDTFRYL GE +VY LR NP +KGEDA +F + +G+ ++D NR + Sbjct: 186 IHETGVHWIDTFRYLIGEPEAVYADLRRLNPAIKGEDAGYFIFDYSNGVRALFDGNRLLD 245 Query: 239 YNAEIRPPAYGKMTLEAELGNLRLDEDGRIFITLRGGTEQEHIYTIPD--GWKGGSVIST 296 + AE G+ LE G L L DG + + G ++ + D G+ G V + Sbjct: 246 HAAENCRTTLGEALLEGTRGTLTLKGDGSVRLRHFGDKDETLLLAAQDWPGFGGDCVKNL 305 Query: 297 HQHFIDGLHSGDSNYFETEGEKYMISQRIVYACYQSAKEQKAI 339 H +DGL G E E Y+ I A Y+S ++ + I Sbjct: 306 ISHVVDGLLDGKP--LENEARSYLKVITIEQAIYESDRKGQKI 346 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,233,717,212 Number of extensions: 99903113 Number of successful extensions: 249682 Number of sequences better than 1.0e-05: 1791 Number of HSP's gapped: 249599 Number of HSP's successfully gapped: 1791 Length of query: 342 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 206 Effective length of database: 1,779,438,163 Effective search space: 366564261578 Effective search space used: 366564261578 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)