BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0021.p (379 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q1YHU1.1 Q1YHU1_MOBAS 3-isopropylmalate dehydrogenase 328 6e-88 Q89GM4.1 Q89GM4_BRAJA LeuB protein 317 1e-84 A8TPC9.1 A8TPC9_9PROT 3-isopropylmalate dehydrogenase EC=1.1.1.85 299 3e-79 A5EFJ2.1 A5EFJ2_BRASB 3-isopropylmalate dehydrogenase EC=1.1.1.85 295 3e-78 C5D1S2.1 C5D1S2_VARPD 3-isopropylmalate dehydrogenase EC=1.1.1.85 295 4e-78 >Q1YHU1.1 Q1YHU1_MOBAS 3-isopropylmalate dehydrogenase Length = 364 Score = 328 bits (841), Expect = 6e-88, Method: Compositional matrix adjust. Identities = 168/365 (46%), Positives = 235/365 (64%), Gaps = 6/365 (1%) Query: 1 MSRYNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMT 60 ++ Y I+ + GDGIGPEVT+ A++VL G+ + GA G + G+ + Sbjct: 4 VTPYRIATIKGDGIGPEVTQAAMRVL---TEACGAGVLDFVMLEGGA--GSHVATGEVLP 58 Query: 61 EEQFGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPG 120 E + A+L GA GL +PDG EV ++ +LRF LDLYA VRP+KL +GV Sbjct: 59 AETAEACRTHHAILHGAAGLPGVVYPDGTEVGNELHLKLRFALDLYANVRPIKLLKGVAS 118 Query: 121 PLREQEKG-IDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXXXX 179 PLR E G IDYV+VREN EGLYASR G +R E+A+D+++ITR GT+++ + Sbjct: 119 PLRAYEGGGIDYVIVRENTEGLYASRGAGVVLRGEMASDSLVITRKGTERVARFAFELAR 178 Query: 180 XXXXXXXXXXSMVTCVDKANVLSSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQP 239 VT DKAN+L SYA+FR+I DEV +YPD+ DYAY DA+ + + +P Sbjct: 179 QRKGALRDGKRRVTVCDKANILRSYAFFRRICDEVHGDYPDVEIDYAYADAITVHLLKRP 238 Query: 240 AQYDVLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANP 299 YDV+VAENMF DI+SDL +AT+GG+G++PSG+IGD H +FQ +HGSAP IAG+ +A+P Sbjct: 239 EFYDVIVAENMFGDIISDLGAATVGGMGISPSGEIGDSHGLFQGAHGSAPDIAGQDLASP 298 Query: 300 IATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDA 359 +A ILSG MML WL ++H D+ L A +EAAV L+ G D+GG++ SE+ DA Sbjct: 299 VAAILSGAMMLRWLADKHDDSTLATAAGRVEAAVDRTLEAGDFVPTDLGGRARCSEMTDA 358 Query: 360 VVQAL 364 +++A+ Sbjct: 359 ILRAM 363 >Q89GM4.1 Q89GM4_BRAJA LeuB protein Length = 359 Score = 317 bits (813), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 168/361 (46%), Positives = 225/361 (62%), Gaps = 6/361 (1%) Query: 4 YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQ 63 Y I+V+ GDGIGPEV A+ VL+A G A + FL+ G + Sbjct: 5 YTIAVVHGDGIGPEVARAAVAVLQA-----GVQAGTLRFVDYPAGADHFLKTGDSFPAAS 59 Query: 64 FGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPLR 123 F + ADA+L GA G+ HPDG E D LRF LDL+A VRP+KLY+GVP PL Sbjct: 60 FEGCRTADAILHGAAGIPGVVHPDGTEAGLDFTLTLRFKLDLFANVRPIKLYKGVPSPL- 118 Query: 124 EQEKGIDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXXXXXXXX 183 + IDYV+VREN EGLYA+R G +R+E+A DT++ TR G ++IV + Sbjct: 119 GRPGPIDYVIVRENSEGLYAARGAGALLREEVAVDTLVQTRKGVERIVRFAFELARTRNG 178 Query: 184 XXXXXXSMVTCVDKANVLSSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQPAQYD 243 VTC DKANVL +YA+FR ++DEVA YPDI ++ VDAM + V +P +D Sbjct: 179 SPKDGRRRVTCCDKANVLRTYAFFRAVFDEVAKEYPDIEAEHVLVDAMTVHLVNKPTHFD 238 Query: 244 VLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANPIATI 303 V+V ENMF DI+SDL +AT+GG+G+APS ++GD FQ SHGSAP IAGK +ANP TI Sbjct: 239 VIVTENMFGDIISDLGAATVGGMGMAPSEEVGDGIGFFQASHGSAPDIAGKGLANPYGTI 298 Query: 304 LSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDAVVQA 363 L+ +ML L ++H+D+ L IEAAV A L++ +RT DIGG + T++V DA+++ Sbjct: 299 LASILMLRRLDQRHRDSRLKLGADRIEAAVCAGLEDPALRTRDIGGVAPTNQVVDALLKR 358 Query: 364 L 364 L Sbjct: 359 L 359 >A8TPC9.1 A8TPC9_9PROT 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 371 Score = 299 bits (765), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 171/364 (46%), Positives = 215/364 (59%), Gaps = 9/364 (2%) Query: 4 YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQ 63 Y I+V+ GDGIGPEV+ ++VL Q S GA F + G A E Sbjct: 13 YRIAVVPGDGIGPEVSAATVRVL--TQALGTSFPIEFRDYDAGAEC--FRRTGTAFPEAT 68 Query: 64 FGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPLR 123 + A A+L GA GL + PDG E D ++R LDLYA VRP++L EG P R Sbjct: 69 QVACRQAHAVLHGAAGLPDVLFPDGTEAGQDFSMKIRAALDLYANVRPIRLMEGAP--RR 126 Query: 124 EQEKGID---YVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXXXXX 180 KG D + +VREN EGLYA+R GG +R +IATDT+++TR G ++IV Y Sbjct: 127 LVGKGPDDIRFTIVRENTEGLYAARGGGNIIRGQIATDTLVMTREGVERIVRYAAELARD 186 Query: 181 XXXXXXXXXSMVTCVDKANVLSSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQPA 240 VT VDKANVL SYA+FR+I D V A+ P I ++ VDAM + + +P Sbjct: 187 GQGAPADGVRRVTIVDKANVLRSYAFFREIADGVLADCPGIEVEHVIVDAMTVHMIERPE 246 Query: 241 QYDVLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANPI 300 +DV+V EN+F DILSDL +A +GG+GLAPSG+IG H FQ SHGSAP IAGK +ANPI Sbjct: 247 HFDVIVCENLFGDILSDLGAAIMGGMGLAPSGEIGRDHGYFQGSHGSAPTIAGKGLANPI 306 Query: 301 ATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDAV 360 A ILSG MMLDWLG++H D AL +A + I A AVLK G T DIGG ST + Sbjct: 307 ACILSGAMMLDWLGKRHSDPALTRAAERIREATGAVLKAGRAVTPDIGGSGSTESCAAEI 366 Query: 361 VQAL 364 L Sbjct: 367 CATL 370 >A5EFJ2.1 A5EFJ2_BRASB 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 359 Score = 295 bits (756), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 161/361 (44%), Positives = 221/361 (61%), Gaps = 10/361 (2%) Query: 5 NISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQF 64 +I+VL GDGIGPEV AL+VLR V+ G + A +G +L G ++ E Sbjct: 8 HIAVLPGDGIGPEVMAPALEVLRKVEAKAG---LAFRFTEAQAGAGNYLATGVSIAESTI 64 Query: 65 GQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPLR- 123 + ADA+L+GA GL R+PD E+ + LRF DLYAGVRP +L GVP P+ Sbjct: 65 RLCEQADAILLGACGLPSVRYPDNTEIAPQI--ELRFHFDLYAGVRPARLIPGVPSPIVG 122 Query: 124 EQEKGIDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXXXXXXXX 183 E+GID+VV+RE+ EGL+AS G +E A +T++ITR ++++ ++ Sbjct: 123 AAERGIDFVVIRESTEGLFASMGKGVVTHEE-ARETLVITRKTSERLFNFSFKLAERRRA 181 Query: 184 XXXXXXSMVTCVDKANVLSSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQPAQYD 243 ++ CVDKANV ++AYFRKI+DEVA +P++ + Y+DA A V +P +D Sbjct: 182 RGRS--GILGCVDKANVFKAFAYFRKIFDEVAELHPNVKAERLYIDACALMLVKRPWDFD 239 Query: 244 VLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANPIATI 303 VLV ENMF DILSDL++ IGG+G+APS DIGDRHA+FQP HG+AP I G+ ANP A I Sbjct: 240 VLVTENMFGDILSDLAAGLIGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMI 299 Query: 304 LSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDAVVQA 363 LS MMLDWL + +A + IE AV V G ++ + GG + T EV AV++A Sbjct: 300 LSAAMMLDWLADTRGIEGAAEAAQRIEQAVDKVYASG-LKPFEFGGSAGTLEVTKAVLEA 358 Query: 364 L 364 L Sbjct: 359 L 359 >C5D1S2.1 C5D1S2_VARPD 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 363 Score = 295 bits (756), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 165/362 (45%), Positives = 217/362 (59%), Gaps = 7/362 (1%) Query: 4 YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQ 63 Y I+VL GDGIG EV A VL AVQ G A + +L G A+ E Sbjct: 5 YRIAVLPGDGIGTEVMHAAQAVLEAVQPRVGRALALEWLP---AGAQHYLDSGVALPETT 61 Query: 64 FGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPL- 122 + ++ADA+L GA+GL R DG E+ + R+ FDL YAGVRP++ + G+P PL Sbjct: 62 LRRCESADAILFGAMGLPHVRGADGTEIIPQLDLRMHFDL--YAGVRPIRTFRGLPTPLS 119 Query: 123 REQEKGIDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXXXXXXX 182 + + ID V+VRE+ EGL+ +R G D+ DT+ ITR GT +I ++ Sbjct: 120 NPKAEHIDLVLVRESTEGLFYARGRGDVRGDQEVFDTMRITRHGTTRICEFAFRLAARRA 179 Query: 183 XXXXXXXSMVTCVDKANVLSSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQPAQY 242 VT VDKANV +S A+FRK+++EVAA +P +A D YVDAMA V++P Y Sbjct: 180 ERLGRAGR-VTNVDKANVFASMAFFRKVFEEVAARHPAVAADSGYVDAMALNLVMKPWTY 238 Query: 243 DVLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANPIAT 302 DVLV ENMF DILSDL +A +GG+G+APSGDIGDRHA+FQP+HG+AP IAG+ ANP A Sbjct: 239 DVLVTENMFGDILSDLIAALVGGMGMAPSGDIGDRHALFQPAHGTAPDIAGQGKANPTAM 298 Query: 303 ILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDAVVQ 362 +LS MML+WL E+ D AL IE AV V G +R + GG T+++ A Sbjct: 299 LLSAAMMLEWLAERSGDTALADGAALIERAVEQVFATGQVRPMEFGGMHGTADITRAATD 358 Query: 363 AL 364 AL Sbjct: 359 AL 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,066,345,214 Number of extensions: 82602977 Number of successful extensions: 215986 Number of sequences better than 1.0e-05: 3459 Number of HSP's gapped: 204809 Number of HSP's successfully gapped: 3767 Length of query: 379 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 242 Effective length of database: 1,770,017,148 Effective search space: 428344149816 Effective search space used: 428344149816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)