BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0028.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGY8.1 B1DGY8_9BACL Binding-protein-dependent transport system... 483 e-135 B1D7X2.1 B1D7X2_9BACL Binding-protein-dependent transport system... 472 e-131 Q9KBL5.1 Q9KBL5_BACHD Transmembrane lipoprotein 472 e-131 B3KDQ7.1 B3KDQ7_9BACI Binding-protein-dependent transport system... 465 e-129 Q5WIY2.1 Q5WIY2_BACSK Polysaccharide ABC transporter permease 440 e-122 >B1DGY8.1 B1DGY8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 305 Score = 483 bits (1244), Expect = e-135, Method: Compositional matrix adjust. Identities = 232/301 (77%), Positives = 266/301 (88%) Query: 44 SAWKELKNQKYLYYMSIPFVLWVFVFQYLPLWGWTMAFQKYKPGVGFFEQKWVGFEHFRT 103 S W+ + Q+YLYYMS+PFVL+VF+F YLPLWGWTMAFQ+++ G FF+QKWVGF++FR Sbjct: 5 SIWQRIVRQRYLYYMSVPFVLFVFLFSYLPLWGWTMAFQQFRVGKFFFDQKWVGFDNFRM 64 Query: 104 LFGDEHFYQVLRNTLAMSFMGLIAGFIVPVVFALLLNEVRVQALKRFVQTVSYLPHFVSW 163 LF D+ F+ LRNTLAMSFMGLIA F +P++FA+LLNEVR+ LKRFVQTVSYLPHFVSW Sbjct: 65 LFQDDQFFNALRNTLAMSFMGLIASFTIPILFAVLLNEVRISFLKRFVQTVSYLPHFVSW 124 Query: 164 VVAAGIVTKMLSTDGGVVNDILLGLNLIDQPIQFMAQGHLFWGVVTSADIWKETGWNAII 223 VV AGIVTKMLS + GVVN ILLGL+LI +PIQFMA+ +LFW +VTSADIWKETGWNAII Sbjct: 125 VVVAGIVTKMLSAENGVVNQILLGLHLIHEPIQFMAKENLFWYLVTSADIWKETGWNAII 184 Query: 224 YLAAISGIGPELYEAARVDGASRWRQIWHITLPGIRPTMIVLLIMSIGHLLGTGFEKQFL 283 YLAAI+GIGPELYEAARVDGASR +QIWHITLPGIR T+I+LLIMSIGHL+ GFEKQFL Sbjct: 185 YLAAIAGIGPELYEAARVDGASRLKQIWHITLPGIRSTIIILLIMSIGHLISIGFEKQFL 244 Query: 284 LGNHLVIDYSEVLDLYALNYGLAMGRYSFGTAINIFNSVISLVLLFAANGIFKRFTNESI 343 LGN LV + SEVLDLYALNYGL MGRYSFGTAINIFNSV+SL+LLF ANGIFK+ T ESI Sbjct: 245 LGNSLVKEKSEVLDLYALNYGLGMGRYSFGTAINIFNSVVSLILLFVANGIFKKITKESI 304 Query: 344 M 344 M Sbjct: 305 M 305 >B1D7X2.1 B1D7X2_9BACL Binding-protein-dependent transport systems inner membrane component Length = 319 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 222/319 (69%), Positives = 265/319 (83%), Gaps = 1/319 (0%) Query: 27 MKTITASA-ENAYKKRKKSAWKELKNQKYLYYMSIPFVLWVFVFQYLPLWGWTMAFQKYK 85 MK +T S + KKR+++ WK +YLY MS+PF++WVFVF Y PLWGWTMAFQK+K Sbjct: 1 MKAVTTSVPQQTRKKRERNFWKTAIRNRYLYMMSLPFIIWVFVFSYYPLWGWTMAFQKFK 60 Query: 86 PGVGFFEQKWVGFEHFRTLFGDEHFYQVLRNTLAMSFMGLIAGFIVPVVFALLLNEVRVQ 145 P F+EQ WVGF+ F+TLF D+ FY V+RNTLAMSFMG+IAGF VP++FA+LLNEVR Sbjct: 61 PAKSFWEQDWVGFDQFKTLFQDDTFYLVMRNTLAMSFMGIIAGFAVPIIFAVLLNEVRSM 120 Query: 146 ALKRFVQTVSYLPHFVSWVVAAGIVTKMLSTDGGVVNDILLGLNLIDQPIQFMAQGHLFW 205 A KRF QTVSYLPHFVSWVV AGIV+KMLS D G VN+IL+ L +ID PIQF+A+ H FW Sbjct: 121 AFKRFAQTVSYLPHFVSWVVVAGIVSKMLSADHGTVNEILMWLGIIDTPIQFLAKPHYFW 180 Query: 206 GVVTSADIWKETGWNAIIYLAAISGIGPELYEAARVDGASRWRQIWHITLPGIRPTMIVL 265 G+VT +D+WKE GWN IIYLAAI+GI E+YEAARVDGA+R RQIWHITLPGIR T+++L Sbjct: 181 GIVTVSDVWKEMGWNTIIYLAAIAGISQEMYEAARVDGANRIRQIWHITLPGIRSTIVIL 240 Query: 266 LIMSIGHLLGTGFEKQFLLGNHLVIDYSEVLDLYALNYGLAMGRYSFGTAINIFNSVISL 325 LIMSIGHL+ GFEKQFLLGN+LV DYS+VLDLYAL YGL M RYSFGTA+NIFNSV+S+ Sbjct: 241 LIMSIGHLIQIGFEKQFLLGNNLVRDYSQVLDLYALEYGLQMSRYSFGTAVNIFNSVVSI 300 Query: 326 VLLFAANGIFKRFTNESIM 344 +LLF ANG+FK+ T ESIM Sbjct: 301 ILLFMANGLFKKITKESIM 319 >Q9KBL5.1 Q9KBL5_BACHD Transmembrane lipoprotein Length = 319 Score = 472 bits (1214), Expect = e-131, Method: Compositional matrix adjust. Identities = 220/319 (68%), Positives = 264/319 (82%), Gaps = 1/319 (0%) Query: 27 MKTITASAENAYK-KRKKSAWKELKNQKYLYYMSIPFVLWVFVFQYLPLWGWTMAFQKYK 85 M+T+ A + + +R+K LK QKYLY MS+PFV WV VF Y PLWGWTMAFQ ++ Sbjct: 1 METVKEKAHSKIRVQRRKKILTTLKQQKYLYLMSLPFVAWVLVFNYFPLWGWTMAFQNFR 60 Query: 86 PGVGFFEQKWVGFEHFRTLFGDEHFYQVLRNTLAMSFMGLIAGFIVPVVFALLLNEVRVQ 145 PG+ FFEQ+WVG +HF LF D FY VLRNTLAMS MGLI GF +P++FA+LLNEVR Sbjct: 61 PGLSFFEQEWVGLQHFIELFQDNRFYLVLRNTLAMSIMGLIVGFTMPILFAVLLNEVRHM 120 Query: 146 ALKRFVQTVSYLPHFVSWVVAAGIVTKMLSTDGGVVNDILLGLNLIDQPIQFMAQGHLFW 205 KR VQTVSYLPHFVSWVV AGIVTKMLS DGG+VN +L+ +DQP+QFMA+G FW Sbjct: 121 VFKRTVQTVSYLPHFVSWVVVAGIVTKMLSIDGGIVNQLLMAFGFVDQPVQFMAKGEWFW 180 Query: 206 GVVTSADIWKETGWNAIIYLAAISGIGPELYEAARVDGASRWRQIWHITLPGIRPTMIVL 265 +VT +D+WKE GWN+II+LAA++GI P+LYEAA+VDGASRWRQIWHITLPGIRPT++V+ Sbjct: 181 VIVTLSDLWKEMGWNSIIFLAAMAGIDPQLYEAAKVDGASRWRQIWHITLPGIRPTILVI 240 Query: 266 LIMSIGHLLGTGFEKQFLLGNHLVIDYSEVLDLYALNYGLAMGRYSFGTAINIFNSVISL 325 LI+SIGHL+ GFEKQFLLGN LV+DYSEVLDLYALNYGL +GRYS+GTAI IFNSV+ + Sbjct: 241 LILSIGHLMAIGFEKQFLLGNPLVVDYSEVLDLYALNYGLGLGRYSYGTAIGIFNSVVCI 300 Query: 326 VLLFAANGIFKRFTNESIM 344 +LLF ANGIFKRFTN+S+M Sbjct: 301 ILLFLANGIFKRFTNQSVM 319 >B3KDQ7.1 B3KDQ7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 320 Score = 465 bits (1197), Expect = e-129, Method: Compositional matrix adjust. Identities = 223/316 (70%), Positives = 259/316 (81%) Query: 29 TITASAENAYKKRKKSAWKELKNQKYLYYMSIPFVLWVFVFQYLPLWGWTMAFQKYKPGV 88 T T S+ K+KK W K QK LY MS+PF++ VF+F YLP+WGW MAFQ YKPG Sbjct: 5 TKTESSIQPAAKKKKGFWTTFKEQKVLYGMSLPFIVIVFIFSYLPVWGWLMAFQNYKPGK 64 Query: 89 GFFEQKWVGFEHFRTLFGDEHFYQVLRNTLAMSFMGLIAGFIVPVVFALLLNEVRVQALK 148 G FEQKWVG +HF LF D+ FY VLRNTLAMSFMGLI GF +P++FA+LLNE+R K Sbjct: 65 GIFEQKWVGLDHFAALFADDKFYLVLRNTLAMSFMGLIVGFTIPILFAVLLNELRGSVFK 124 Query: 149 RFVQTVSYLPHFVSWVVAAGIVTKMLSTDGGVVNDILLGLNLIDQPIQFMAQGHLFWGVV 208 R VQT+SYLPHFVSWVV AGIVTKMLSTDGG VN +L+ L LIDQPIQFMAQG+LFW +V Sbjct: 125 RTVQTISYLPHFVSWVVVAGIVTKMLSTDGGAVNQLLMWLGLIDQPIQFMAQGNLFWYIV 184 Query: 209 TSADIWKETGWNAIIYLAAISGIGPELYEAARVDGASRWRQIWHITLPGIRPTMIVLLIM 268 T +DIWKE GW+AIIYLAAI+GI E +EAA+VDGASR RQIWHITLP I PT+ +LLIM Sbjct: 185 TGSDIWKEMGWSAIIYLAAITGIDQEQFEAAKVDGASRIRQIWHITLPSIAPTIAILLIM 244 Query: 269 SIGHLLGTGFEKQFLLGNHLVIDYSEVLDLYALNYGLAMGRYSFGTAINIFNSVISLVLL 328 SIGHL+ GFEKQFLLGN LV+DYSEVLDLYALNYGL MGR+SFGTAI +FNSV+S++LL Sbjct: 245 SIGHLISIGFEKQFLLGNPLVVDYSEVLDLYALNYGLGMGRFSFGTAIGMFNSVVSIILL 304 Query: 329 FAANGIFKRFTNESIM 344 ANG+FK+FT +SIM Sbjct: 305 LLANGVFKKFTKQSIM 320 >Q5WIY2.1 Q5WIY2_BACSK Polysaccharide ABC transporter permease Length = 321 Score = 440 bits (1132), Expect = e-122, Method: Compositional matrix adjust. Identities = 207/321 (64%), Positives = 261/321 (81%), Gaps = 3/321 (0%) Query: 27 MKTITASAEN---AYKKRKKSAWKELKNQKYLYYMSIPFVLWVFVFQYLPLWGWTMAFQK 83 MK I A + A +K+S + + Q+YLY MS+PFV WVFVF YLPL+GWTMAFQ Sbjct: 1 MKLIKKKAHSVAAAPLGQKRSFRQTVWQQRYLYAMSLPFVAWVFVFHYLPLFGWTMAFQD 60 Query: 84 YKPGVGFFEQKWVGFEHFRTLFGDEHFYQVLRNTLAMSFMGLIAGFIVPVVFALLLNEVR 143 Y+PG+ FFEQ+WVGF+HF LF DE FY VLRNTL MS +GL+ GF P++FA+LLNE R Sbjct: 61 YRPGLAFFEQEWVGFKHFIELFQDERFYLVLRNTLVMSLLGLLVGFTFPILFAVLLNEAR 120 Query: 144 VQALKRFVQTVSYLPHFVSWVVAAGIVTKMLSTDGGVVNDILLGLNLIDQPIQFMAQGHL 203 + K+ VQTVSYLPHFVSWVV AGIVTKMLS DGG+VN++L+ D+PIQFM +G Sbjct: 121 AKLFKKTVQTVSYLPHFVSWVVVAGIVTKMLSVDGGIVNEVLVATGTADKPIQFMTKGEW 180 Query: 204 FWGVVTSADIWKETGWNAIIYLAAISGIGPELYEAARVDGASRWRQIWHITLPGIRPTMI 263 FW +VT AD+WKE GWN+II+LAA++G+ P+LYEAA+VDGASRWRQIWHITLPGIRPT++ Sbjct: 181 FWIIVTLADLWKEMGWNSIIFLAAMAGVDPQLYEAAKVDGASRWRQIWHITLPGIRPTIL 240 Query: 264 VLLIMSIGHLLGTGFEKQFLLGNHLVIDYSEVLDLYALNYGLAMGRYSFGTAINIFNSVI 323 V+LI+SIG L+ GFEKQFLLGN V++YSEVLDLYALNYG+ + R+S+GTAI IFNS++ Sbjct: 241 VVLILSIGSLMTIGFEKQFLLGNPTVVNYSEVLDLYALNYGINLSRFSYGTAIGIFNSLV 300 Query: 324 SLVLLFAANGIFKRFTNESIM 344 +++LLF ANG+FKR+ N+S+M Sbjct: 301 AIILLFIANGLFKRYANQSVM 321 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,115,806,849 Number of extensions: 89084597 Number of successful extensions: 320418 Number of sequences better than 1.0e-05: 5159 Number of HSP's gapped: 317245 Number of HSP's successfully gapped: 5159 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)