BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0026.p (594 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDQ9.1 B3KDQ9_9BACI Histidine kinase internal region 509 e-142 B1D7X4.1 B1D7X4_9BACL Integral membrane sensor signal transducti... 496 e-138 Q9KBL7.1 Q9KBL7_BACHD Two-component sensor histidine kinase 414 e-113 Q5WIY4.1 Q5WIY4_BACSK Two-component sensor histidine kinase 379 e-103 B8I617.1 B8I617_CLOCE Histidine kinase 302 5e-80 >B3KDQ9.1 B3KDQ9_9BACI Histidine kinase internal region Length = 602 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 268/592 (45%), Positives = 389/592 (65%), Gaps = 1/592 (0%) Query: 3 LVRIMNDMKMKKKLAVTFISAAVXXXXXXXXXXXXXXREIVINDAFTQLSNNVERVRKRT 62 ++++NDMK++ KL ++F+ R+ ++A Q NV+RV+KR+ Sbjct: 5 FIQLINDMKIRNKLILSFVVVVFVPVAIVGIFLTGELRKFAFDNALEQAYQNVDRVKKRS 64 Query: 63 EELIKVPLNISYRLTIDNRMKKVAAQKYESYTEVIQAYREYTDIRDYIQLYKEISSIRVY 122 E+I V ++SYRL+ D R++ +A ++YES +V AYREY D++ I++YKEIS+IR Y Sbjct: 65 TEVINVADDLSYRLSYDERLRNLANRQYESVYDVFVAYREYPDLQQAIRMYKEISNIRFY 124 Query: 123 VVNPGALNNWEFIQPDNAIMAEGWYKEAIEQKGLAGWNLIKDERNGSEQLSLVRSFPVDS 182 NP LNNWEF+ P + I + WY+ A + G+ W+ I+DER+ LSL++ + S Sbjct: 125 SDNPTMLNNWEFLYPKDEIRSTEWYRRAEDDIGVIYWDYIEDERDQKVYLSLIKGVDLGS 184 Query: 183 LGEKGVLAINVDKQQLRSILDQEPFTTLIVDDRNRVVGSNEGEFFGKNLSEIDGDAKILS 242 +GVL INV+ L +IL QE F T+IVD N +V +N E +GK L+EI+ + + Sbjct: 185 KDNRGVLVINVNTGMLNTILSQEAFDTIIVDSSNNIVAANRPELYGKTLAEIEDASGAVY 244 Query: 243 RQEGSYDTVVNGKASKVVMVNLNPENSWNGLRIISIFSVSEITREANQVIRLGAVVITVS 302 GSY++V++ +ASKV++ LN E+ NGLRIISIFS+ I +AN++ +L VI +S Sbjct: 245 HASGSYESVIHDEASKVLIEPLNTESGVNGLRIISIFSIDSIVADANRISKLAMTVIIIS 304 Query: 303 LMFAVLLIYXXXXXXXXXXXXXXKHMSKAGAGSWETYIHIDSKDEIGLLSKQFNALIRSV 362 L+ AVLLIY KH++K G G+ + ID KDEIG LS+QFN+++ +V Sbjct: 305 LLLAVLLIYSFSSLISNRLLRLSKHITKVGTGNLGVAMEIDGKDEIGQLSRQFNSMVGNV 364 Query: 363 HDLVQEVQETNHQKNLVEQRQSEMKFKMLASQIHPHFLFNSLESIRMEAHIRRQDEIAEA 422 +DL+ EVQE+N QK L+EQ+Q+E+KFKM+ASQI+PHFLFN+LESIRMEAH++ + EIA Sbjct: 365 NDLMNEVQESNEQKRLMEQKQNEIKFKMMASQINPHFLFNALESIRMEAHLKGETEIARI 424 Query: 423 VWLLSTLLRSSLEAGNGNIPLREELERVRCYLEMQKFRYEDRLEYHLTVDADLEVMPVPP 482 V LL ++RS+LE G+G +++E+E V+CYL++QKFRYEDRL+Y L VD + + +PP Sbjct: 425 VRLLGKMMRSNLEVGSGKTTIKDEIEMVQCYLDIQKFRYEDRLKYELQVDPEANRVRIPP 484 Query: 483 LIIQPLVENSVLHGLDNREEGAVITVEVKKMPKGARVKVCDNGAGFTAERLAEIQAELAE 542 LIIQPLVEN+V+HG+D++EEG I V V V DNGAG + ++L I L + Sbjct: 485 LIIQPLVENAVIHGMDHKEEGTSIKVRVTASEGKLHVMTEDNGAGISLDKLQMIYGYLND 544 Query: 543 SVHEQESKRIGMRNVNDRLVLLYGESSALSIESGSGTGTAITFFIPGGALND 594 + E++ RIG+RNV+ RL L YG + L I S G GT I F IP G +D Sbjct: 545 T-DEKDGGRIGLRNVHVRLQLTYGPEAGLIIYSEPGIGTRIEFSIPTGGESD 595 >B1D7X4.1 B1D7X4_9BACL Integral membrane sensor signal transduction histidine kinase Length = 595 Score = 496 bits (1278), Expect = e-138, Method: Compositional matrix adjust. Identities = 257/585 (43%), Positives = 370/585 (63%), Gaps = 2/585 (0%) Query: 6 IMNDMKMKKKLAVTFISAAVXXXXXXXXXXXXXXREIVINDAFTQLSNNVERVRKRTEEL 65 ++N++K++ KL ++ I A R + +++A Q NV+RV+ RT EL Sbjct: 8 LLNNVKLRTKLILSCIVVAFIPILIVGVFLTNELRNMALDNAMEQTHTNVQRVKARTTEL 67 Query: 66 IKVPLNISYRLTIDNRMKKVAAQKYESYTEVIQAYREYTDIRDYIQLYKEISSIRVYVVN 125 + V + +YRL ++R++ V +KYES EV++AYREYTD + Q + E+++IR Y+ N Sbjct: 68 MNVAYDSAYRLE-NSRLEAVTNRKYESTYEVVKAYREYTDFNEIRQQHDEVANIRFYMEN 126 Query: 126 PGALNNWEFIQPDNAIMAEGWYKEAIEQKGLAGWNLIKDERNGSEQLSLVRSFPVDSLGE 185 P LNNW+FIQPDN++ WY A+ W ++DER+ LSLVR S Sbjct: 127 PTMLNNWDFIQPDNSMKETVWYANALRPGSGVNWYYLEDERDKRNYLSLVRGISFSSNNT 186 Query: 186 KGVLAINVDKQQLRSILDQEPFTTLIVDDRNRVVGSNEGEFFGKNLSEIDGDAKILSRQE 245 GVL I ++ Q L SIL+QE F T+IVDD NR+V +N GK L EID + I+ Q Sbjct: 187 SGVLVITLNMQALNSILNQESFETMIVDDNNRIVAANRMGRAGKTLQEIDYEQSIVEEQS 246 Query: 246 GSYDTVVNGKASKVVMVNLNPENSWNGLRIISIFSVSEITREANQVIRLGAVVITVSLMF 305 G Y ++G+AS+V++ L PE S N LRIIS+FSV I +AN++ L VI++SL+ Sbjct: 247 GMYSANIDGQASRVLIEPLIPEASGNTLRIISVFSVDTIVADANRINSLALTVISISLVI 306 Query: 306 AVLLIYXXXXXXXXXXXXXXKHMSKAGAGSWETYIHIDSKDEIGLLSKQFNALIRSVHDL 365 A+ LI KH++K G + + ID KDEIG LS+QFN+++ S++ L Sbjct: 307 AIFLIIGVSTIITKRMLRLSKHITKVATGKLDIVMEIDGKDEIGQLSRQFNSMVSSINGL 366 Query: 366 VQEVQETNHQKNLVEQRQSEMKFKMLASQIHPHFLFNSLESIRMEAHIRRQDEIAEAVWL 425 V+EVQE+N K +E +Q+E+K KM+ASQI+PHFLFN+LESIRM+AH++ + EI++ V L Sbjct: 367 VEEVQESNKMKRQLEAKQNEIKLKMMASQINPHFLFNALESIRMKAHLKGEKEISQVVRL 426 Query: 426 LSTLLRSSLEAGNGNIPLREELERVRCYLEMQKFRYEDRLEYHLTVDADLEVMPVPPLII 485 L L+R S+E PL +E++ VRCYL++QKFRYEDRL Y L VD +E + +PPLII Sbjct: 427 LGKLMRRSIEVSQRLTPLADEMDIVRCYLDIQKFRYEDRLSYELHVDPVVERVSIPPLII 486 Query: 486 QPLVENSVLHGLDNREEGAVITVEVKKMPKGARVKVCDNGAGFTAERLAEIQAELAESVH 545 QPLVEN+V+HG +N+ EG ++TV + RV V DNGAG T E++ ++ L E+ Sbjct: 487 QPLVENAVIHGFENKVEGGMVTVRAVREGDYVRVDVIDNGAGMTREKIDKLTQHLNET-E 545 Query: 546 EQESKRIGMRNVNDRLVLLYGESSALSIESGSGTGTAITFFIPGG 590 + E+ RIG+RNV+ RL L YG L ES G GT ++F +P G Sbjct: 546 DAENNRIGLRNVHLRLQLTYGPEHGLQYESEPGIGTKVSFTLPFG 590 >Q9KBL7.1 Q9KBL7_BACHD Two-component sensor histidine kinase Length = 597 Score = 414 bits (1064), Expect = e-113, Method: Compositional matrix adjust. Identities = 231/586 (39%), Positives = 342/586 (58%), Gaps = 3/586 (0%) Query: 3 LVRIMNDMKMKKKLAVTFISAAVXXXXXXXXXXXXXXREIVINDAFTQLSNNVERVRKRT 62 L++ +NDMK++ KL F+ R I DA Q NVERV++RT Sbjct: 5 LIQTINDMKLRDKLMFMFVVVVFFPMMIVGIFLTNELRSNAIQDAIQQSRVNVERVKERT 64 Query: 63 EELIKVPLNISYRLTIDNRMKKVAAQKYESYTEVIQAYREYTDIRDYIQLYKEISSIRVY 122 E++ VP+ I+ L D R ++ Q+YE+ +V+ AY +Y + +Y+Q Y EI +++ Y Sbjct: 65 TEVLNVPVLIANNLQFDERFIELVNQEYETTYDVVDAYLQYMNFTNYLQYYSEIHNMKYY 124 Query: 123 VVNPGALNNWEFIQPDNAIMAEGWYKEAIEQKGLAGWNLIKDE-RNGSEQLSLVRSFPVD 181 N +NNW I P E W+KEA+ W ++DE + LS+V+ Sbjct: 125 SENTSLMNNWVII-PVEHEAGEEWFKEAMADPAYYRWFYLEDETKKNQAYLSMVKRLNFL 183 Query: 182 SLGEKGVLAINVDKQQLRSILDQEPFTTLIVDDRNRVVGSNEGEFFGKNLSEIDGDAKIL 241 G GVL I VD ++L IL QE FTT+IVD+RNR+ +N+ E+ GK+L E+ +++ Sbjct: 184 EYGSAGVLVITVDPRKLDRILQQESFTTMIVDERNRIAATNQREYIGKDLHEVIDFHEMI 243 Query: 242 SRQEGSYDTVVNGKASKVVMVNLNPENSWNGLRIISIFSVSEITREANQVIRLGAVVITV 301 Y+ +NG+ S++++ L + S + L+IISI + I ++A ++ +GA V Sbjct: 244 QEGPAIYEGKMNGEPSQIIVDYLTLQMSQSELKIISIVTKEHIMKDAKRLSIMGATVTMG 303 Query: 302 SLMFAVLLIYXXXXXXXXXXXXXXKHMSKAGAGSWETYIHIDSKDEIGLLSKQFNALIRS 361 + AVLLI + +++ +G +T I +D KDEIG LS QFN ++ + Sbjct: 304 GMAVAVLLIVHFSWLISKRLSHLSERINQVASGDLKTKIVVDGKDEIGQLSVQFNQMVAN 363 Query: 362 VHDLVQEVQETNHQKNLVEQRQSEMKFKMLASQIHPHFLFNSLESIRMEAHIRRQDEIAE 421 + L+ +V ETN QK L+E+ Q+E+K KMLASQI+PHFLFN+LESIRM++H++ + EIA+ Sbjct: 364 LRSLIHQVHETNRQKRLLEKSQNEIKLKMLASQINPHFLFNTLESIRMKSHMKGETEIAK 423 Query: 422 AVWLLSTLLRSSLEAGNGNIPLREELERVRCYLEMQKFRYEDRLEYHLTVDADLEVMPVP 481 V L L+R SLE +IPLR EL+ VRCYLE+Q FRY DRL Y L +D E++ + Sbjct: 424 VVKQLGKLMRKSLEVTGHHIPLRNELDMVRCYLEIQTFRYGDRLHYELYIDPQSEMVEIL 483 Query: 482 PLIIQPLVENSVLHGLDNREEGAVITVEVKKMPKGARVKVCDNGAGFTAERLAEIQAELA 541 PLIIQPLVEN+V+HGL+ E+G +T+ V V D+G G E+L IQ L Sbjct: 484 PLIIQPLVENAVIHGLERTEDGGTVTISTIVNGNDLTVIVNDDGCGMDEEKLEAIQNMLH 543 Query: 542 ESVHEQESKRIGMRNVNDRLVLLYGESSALSIESGSGTGTAITFFI 587 E + +IG+ NV+ RL L YG++S L IES G GT + F I Sbjct: 544 HP-QEVDGNKIGLLNVHKRLQLTYGKTSGLIIESAKGKGTCVRFTI 588 >Q5WIY4.1 Q5WIY4_BACSK Two-component sensor histidine kinase Length = 599 Score = 379 bits (973), Expect = e-103, Method: Compositional matrix adjust. Identities = 219/586 (37%), Positives = 326/586 (55%), Gaps = 10/586 (1%) Query: 7 MNDMKMKKKLAVTFISAAVXXXXXXXXXXXXXXREIVINDAFTQLSNNVERVRKRTEELI 66 +N +K++ KL + F+S+ V + I DA Q NVERV++R E++ Sbjct: 13 LNQIKIRDKLILMFLSSVVIPIAVVGLFLTNELKANAIKDAQEQADMNVERVKQRINEVL 72 Query: 67 KVPLNISYRLTIDNRMKKVAAQKYESYTEVIQAYREYTDIRDYIQLYKEISSIRVYVVNP 126 K P+ ++ L D R+ ++ YE V QAYR Y D + + EI +IR+YV NP Sbjct: 73 KGPIMVANHLQFDERLSELVNTDYEHSFSVFQAYRSYPDFAYHKGNHPEIETIRLYVDNP 132 Query: 127 GALNNWEFIQPDNAIMAEGWYKEAIEQKGLAGWNLIKDE-RNGSEQLSLVRSFPVDSLGE 185 LNNW FI + + MA WY A G W ++DE + G LSL++ G Sbjct: 133 TLLNNWTFIPVERSQMA-SWYTIAKADVGFYQWFYLQDETQGGGHYLSLMKRINFIEYGT 191 Query: 186 KGVLAINVDKQQLRSILDQEPFTTLIVDDRNRVVGSNEGEFFGKNLSEIDGDAKILSRQE 245 G+L I + L +I+ QE T++VD++N ++ S+E F G+NL E+ + R E Sbjct: 192 FGLLVITIAPDLLETIMQQETHQTVVVDEQNSIISSSEPSFIGENLDEL---VVVTDRGE 248 Query: 246 GS---YDTVVNGKASKVVMVNLNPENSWNGLRIISIFSVSEITREANQVIRLGAVVITVS 302 G Y+ NG+ ++ +L PE S N LRI+SI +I A ++ G +V + Sbjct: 249 GKHGFYEGQANGEPIHMMETSLFPELSQNQLRIVSIIHTEDIVGAAKKLSYFGVLVTGLG 308 Query: 303 LMFAVLLIYXXXXXXXXXXXXXXKHMSKAGAGSWETYIHIDSKDEIGLLSKQFNALIRSV 362 + F++ LI M + G+ T + +D DEIG +S Q N +I + Sbjct: 309 IAFSLFLIVWFSWGYVNRLSRLSATMKEVAQGNLTTKVALDGTDEIGQISLQLNKMIDDL 368 Query: 363 HDLVQEVQETNHQKNLVEQRQSEMKFKMLASQIHPHFLFNSLESIRMEAHIRRQDEIAEA 422 ++ + ++ E N QK +E++Q+EMKFKM+ASQ++PHFLFN+LE+IRM AH+ + +IA+A Sbjct: 369 NESIAQLLEANEQKTALERKQNEMKFKMMASQMNPHFLFNTLEAIRMRAHMSNETDIAKA 428 Query: 423 VWLLSTLLRSSLEAGNGNIPLREELERVRCYLEMQKFRYEDRLEYHLTVDADLEVMPVPP 482 V L LLR SLE G+ IPL+EELE VR YLE+Q FRY +RL Y + ++ + V P Sbjct: 429 VKRLGQLLRKSLEVGSSMIPLKEELEMVRSYLEIQSFRYGERLTYDMDDAVTIDNVAVIP 488 Query: 483 LIIQPLVENSVLHGLDNREEGAVITVEVKKMPKGARVKVCDNGAGFTAERLAEIQAELAE 542 L IQPLVEN+V HGL+ +E + + +K+ G ++V DNG G +R E+QA + E Sbjct: 489 LTIQPLVENAVSHGLEAKEADGYVNIRIKQAEDGTWIEVSDNGIGMGPQRQQEVQA-MIE 547 Query: 543 SVHEQESKRIGMRNVNDRLVLLYGESSALSIESGSGTGTAITFFIP 588 E+ RIG+RNV+ RL YG + L I S G GT + F IP Sbjct: 548 DPLEKPENRIGLRNVHQRLKQTYG-TDGLLISSSQGEGTTVRFLIP 592 >B8I617.1 B8I617_CLOCE Histidine kinase Length = 612 Score = 302 bits (774), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 182/543 (33%), Positives = 291/543 (53%), Gaps = 13/543 (2%) Query: 57 RVRKRTEELIKVPLNISYRLTIDNRMKKVAAQKYESYTEVIQAYREYTDIRDYIQLYKEI 116 RVR R EL+ + NIS ++ D ++K + + KY S+ E A + Y +++YIQ Y +I Sbjct: 60 RVRDRVNELLYLMTNISDKIYFDKQLKNIISTKYSSFLEFNNACKSYPALKEYIQYYSQI 119 Query: 117 SSIRVYVVNPGALNNWEFIQPDNAIMAEGWYKEAIEQKGLAGWNLIKDERNGSEQLSLVR 176 +I +YV N +N F++ + + E WYK A+ G W + N S L+LVR Sbjct: 120 EAITLYVDNSTMWDNGFFVKTTDTVRKEDWYKAAVRSGGNMIWQCFYNNDNNSYYLALVR 179 Query: 177 SFPVDSLGEKGVLAINVDKQQLRSILDQEPFTTLIVDDRNRVVGSNEGEFFG-----KNL 231 +D G V+ + V L S++ E T L+ D +R+V +++ + G K L Sbjct: 180 RIRIDDNGNYAVMVMRVSYSYLTSVIQNEKTTMLMTVDNDRIVLADDRKKLGTKIDLKGL 239 Query: 232 SEIDGDAKILSRQEGSYDTVVNGKASKVVMVNLNPENSWNGLRIISIFSVSEITREANQV 291 + + GD++ + D + +V + P+ + + ++II+I S+ITR+ Sbjct: 240 TAVSGDSEQFLK-----DVSYENRKVFIVHDSFLPQKTRSTIQIINITPTSDITRQVIDT 294 Query: 292 IRLGAVVITVSLMFAVLLIYXXXXXXXXXXXXXXKHMSKAGAGSWETYIHIDSKDEIGLL 351 G ++ +SL+ V+L+ + +A +G + I DEI L Sbjct: 295 YTTGILIALISLVAPVMLMVLFTGNFSKRILFIRTQIHRAASGDLDISPGISGSDEIAEL 354 Query: 352 SKQFNALIRSVHDLVQEVQETNHQKNLVEQRQSEMKFKMLASQIHPHFLFNSLESIRMEA 411 K +I + L+QE E Q+ + +Q+E +FKMLASQI+PHFLFN+LESIRM+A Sbjct: 355 YKDLIKMIGDMKKLLQENYEHKLQQQKLLTKQNEAQFKMLASQINPHFLFNTLESIRMKA 414 Query: 412 HIRRQDEIAEAVWLLSTLLRSSLEAGNGNIPLREELERVRCYLEMQKFRYEDRLEYHLTV 471 + Q EIA + LL +RS LE + PL+ EL+ VR YLE+QKFR+ +R++Y + V Sbjct: 415 FVCGQREIATVIKLLGKSMRSLLELDDNPQPLKSELDFVRNYLEIQKFRFAERVDYTIDV 474 Query: 472 D--ADLEVMPVPPLIIQPLVENSVLHGLDNREEGAVITVEVKKMPKGARVKVCDNGAGFT 529 D +++ V PL+IQP+VEN+ +HGL+ +E I + V + + V V D+G G Sbjct: 475 QDGIDEKILKVLPLLIQPIVENAFVHGLEQKEGHGRIDIIVNRYNENIMVIVKDDGLGME 534 Query: 530 AERLAEIQAELAESVHEQESKRIGMRNVNDRLVLLYGESSALSIESGSGTGTAITFFIPG 589 AE+L E+ ++ ES + K IG+ NVN R+ L YGES + ++S GTGT + P Sbjct: 535 AEKLEEVHCKIKES-GGKTGKSIGLSNVNQRIKLYYGESYGIRVDSSPGTGTTVWISFPE 593 Query: 590 GAL 592 L Sbjct: 594 QCL 596 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,067,566,294 Number of extensions: 120903924 Number of successful extensions: 374459 Number of sequences better than 1.0e-05: 2247 Number of HSP's gapped: 371029 Number of HSP's successfully gapped: 2249 Length of query: 594 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 452 Effective length of database: 1,722,912,073 Effective search space: 778756256996 Effective search space used: 778756256996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)