BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0024.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9U6M1.1 A9U6M1_PHYPA Predicted protein (Fragment) 414 e-114 Q9ALJ4.1 Q9ALJ4_BACST Alpha-galactosidase AgaA 291 4e-77 Q5KXZ9.1 Q5KXZ9_GEOKA Alpha-galactosidase 290 1e-76 Q934H7.1 Q934H7_BACST Alpha-galactosidase AgaB 290 1e-76 A4IQ36.1 A4IQ36_GEOTN Alpha-galactosidase 290 1e-76 >A9U6M1.1 A9U6M1_PHYPA Predicted protein (Fragment) Length = 574 Score = 414 bits (1065), Expect = e-114, Method: Compositional matrix adjust. Identities = 201/241 (83%), Positives = 212/241 (87%) Query: 20 YSGLTVVLSYSIFADRNVIIRSARLGNQGEKKLRLRRALSASVDFPGTRGFEITYLSGAW 79 YSGL VVL YSIF RNVIIRSARL N+G+KKLRLRRALSASVDF G E+ YLSGAW Sbjct: 86 YSGLKVVLLYSIFTARNVIIRSARLINEGDKKLRLRRALSASVDFSGHSDLEMVYLSGAW 145 Query: 80 AREGNITRKPLHQGEVRIDSKRGMSSHQFNPFAALVTPETTESQGEAFGFSLVYSGGFEA 139 AREGN+ R+PL QGE RIDSKRGMSSHQFNPFA+LVTPETTE GEAFGFSLVYSGGFEA Sbjct: 146 AREGNMKRRPLEQGETRIDSKRGMSSHQFNPFASLVTPETTERHGEAFGFSLVYSGGFEA 205 Query: 140 AAEVDSFGSTRLSIGINSFDFAWLLNPGESFQTPETVMVYSGQGLGDMSRTYHRLYRTRL 199 AEVDSFGSTR SIG+NSFDFAW LNPGESFQTPE VMVYS QGLG+MSRTYHRLYRTRL Sbjct: 206 GAEVDSFGSTRFSIGMNSFDFAWQLNPGESFQTPEAVMVYSAQGLGEMSRTYHRLYRTRL 265 Query: 200 CRGEYRDEERPILVNNWEATYFDFNADKLAAIAEEGAKLGIELFVLDDGWVGEXXXXNGM 259 CRG+YRDEERPILVNNWEATYFDFNADKL +IAEEGAKLGIELFVLDDGW G+ N Sbjct: 266 CRGKYRDEERPILVNNWEATYFDFNADKLVSIAEEGAKLGIELFVLDDGWFGKRDADNSS 325 Query: 260 L 260 L Sbjct: 326 L 326 >Q9ALJ4.1 Q9ALJ4_BACST Alpha-galactosidase AgaA Length = 729 Score = 291 bits (746), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 144/231 (62%), Positives = 166/231 (71%), Gaps = 1/231 (0%) Query: 22 GLTVVLSYSIFADRNVIIRSARLGNQGEKKLRLRRALSASVDFPGTRGFEITYLSGAWAR 81 GL V L Y+ + NVI RSAR N+G ++L+L RALS SVDFP T ++ +L GAW R Sbjct: 143 GLEVTLQYTAYEKWNVITRSARFENKGGERLKLLRALSMSVDFP-TADYDWIHLPGAWGR 201 Query: 82 EGNITRKPLHQGEVRIDSKRGMSSHQFNPFAALVTPETTESQGEAFGFSLVYSGGFEAAA 141 E I R+PL G +S+RG SSHQ NPF ALV E QGE +GFS VYSG F A Sbjct: 202 ERWIERRPLVTGVQAAESRRGASSHQQNPFIALVAKNADEHQGEVYGFSFVYSGNFLAQI 261 Query: 142 EVDSFGSTRLSIGINSFDFAWLLNPGESFQTPETVMVYSGQGLGDMSRTYHRLYRTRLCR 201 EVD FG+ R+S+GIN FDF WLL PGESFQTPE VMVYS QGL MS+TYH LYRTRL R Sbjct: 262 EVDQFGTARVSMGINPFDFTWLLQPGESFQTPEVVMVYSDQGLNGMSQTYHELYRTRLAR 321 Query: 202 GEYRDEERPILVNNWEATYFDFNADKLAAIAEEGAKLGIELFVLDDGWVGE 252 G +RD ERPIL+NNWEATYFDFN +K+ IA A+LGIEL VLDDGW GE Sbjct: 322 GAFRDRERPILINNWEATYFDFNEEKIVNIARTAAELGIELVVLDDGWFGE 372 >Q5KXZ9.1 Q5KXZ9_GEOKA Alpha-galactosidase Length = 729 Score = 290 bits (742), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 143/231 (61%), Positives = 167/231 (72%), Gaps = 1/231 (0%) Query: 22 GLTVVLSYSIFADRNVIIRSARLGNQGEKKLRLRRALSASVDFPGTRGFEITYLSGAWAR 81 GL V L Y+ + NVI R+AR N+G ++L+L RALS SVDFP T ++ +L GAW R Sbjct: 143 GLEVTLQYTAYEKWNVITRAARFENKGGERLKLLRALSMSVDFP-TADYDWIHLPGAWGR 201 Query: 82 EGNITRKPLHQGEVRIDSKRGMSSHQFNPFAALVTPETTESQGEAFGFSLVYSGGFEAAA 141 E I R+PL G +S+RG SSHQ NPF ALV E QGE +GFS VYSG F A Sbjct: 202 ERWIERRPLVTGVQAAESRRGASSHQQNPFIALVAKNADEHQGEVYGFSFVYSGNFLAQV 261 Query: 142 EVDSFGSTRLSIGINSFDFAWLLNPGESFQTPETVMVYSGQGLGDMSRTYHRLYRTRLCR 201 EVD F + R+S+GIN FDF WLL PGESFQTPE VMVYS QGL MS+TYH LYRTRL R Sbjct: 262 EVDQFHTARVSMGINPFDFTWLLQPGESFQTPEVVMVYSDQGLNGMSQTYHELYRTRLAR 321 Query: 202 GEYRDEERPILVNNWEATYFDFNADKLAAIAEEGAKLGIELFVLDDGWVGE 252 G +RD ERPIL+NNWEATYFDFN +KL IA+ A+LGIELFVLDDGW G+ Sbjct: 322 GAFRDRERPILINNWEATYFDFNEEKLVNIAKTEAELGIELFVLDDGWFGK 372 >Q934H7.1 Q934H7_BACST Alpha-galactosidase AgaB Length = 729 Score = 290 bits (742), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 143/231 (61%), Positives = 167/231 (72%), Gaps = 1/231 (0%) Query: 22 GLTVVLSYSIFADRNVIIRSARLGNQGEKKLRLRRALSASVDFPGTRGFEITYLSGAWAR 81 GL V L Y+ + NVI R+AR N+G ++L+L RALS SVDFP T ++ +L GAW R Sbjct: 143 GLEVTLQYTAYEKWNVITRAARFENKGGERLKLLRALSMSVDFP-TADYDWIHLPGAWGR 201 Query: 82 EGNITRKPLHQGEVRIDSKRGMSSHQFNPFAALVTPETTESQGEAFGFSLVYSGGFEAAA 141 E I R+PL G +S+RG SSHQ NPF ALV E QGE +GFS VYSG F A Sbjct: 202 ERWIERRPLVTGVQAAESRRGASSHQQNPFIALVAKNADEHQGEVYGFSFVYSGNFLAQV 261 Query: 142 EVDSFGSTRLSIGINSFDFAWLLNPGESFQTPETVMVYSGQGLGDMSRTYHRLYRTRLCR 201 EVD F + R+S+GIN FDF WLL PGESFQTPE VMVYS QGL MS+TYH LYRTRL R Sbjct: 262 EVDQFHTARVSMGINPFDFTWLLQPGESFQTPEVVMVYSDQGLNGMSQTYHELYRTRLAR 321 Query: 202 GEYRDEERPILVNNWEATYFDFNADKLAAIAEEGAKLGIELFVLDDGWVGE 252 G +RD ERPIL+NNWEATYFDFN +KL IA+ A+LGIELFVLDDGW G+ Sbjct: 322 GAFRDRERPILINNWEATYFDFNEEKLVNIAKTEAELGIELFVLDDGWFGK 372 >A4IQ36.1 A4IQ36_GEOTN Alpha-galactosidase Length = 729 Score = 290 bits (742), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 142/231 (61%), Positives = 166/231 (71%), Gaps = 1/231 (0%) Query: 22 GLTVVLSYSIFADRNVIIRSARLGNQGEKKLRLRRALSASVDFPGTRGFEITYLSGAWAR 81 GL V L Y+++ NVI R+ R N G ++L+L RALS S+DFP ++ +L GAWAR Sbjct: 143 GLRVTLQYTVYEKWNVITRAVRFENNGNERLKLLRALSMSIDFPDA-DYDWIHLPGAWAR 201 Query: 82 EGNITRKPLHQGEVRIDSKRGMSSHQFNPFAALVTPETTESQGEAFGFSLVYSGGFEAAA 141 E + R PL G +S+RG SSHQ NPF ALV T E QGE +GFSLVYSG F A Sbjct: 202 ERWVERCPLLTGVQAAESRRGASSHQQNPFIALVRKNTDEHQGEVYGFSLVYSGHFLAQI 261 Query: 142 EVDSFGSTRLSIGINSFDFAWLLNPGESFQTPETVMVYSGQGLGDMSRTYHRLYRTRLCR 201 EVD F + R+S+GIN FDF WLL PGESFQTPE VMVYS QGL MS+TYH LYRTRL R Sbjct: 262 EVDQFQTARVSMGINPFDFTWLLEPGESFQTPEVVMVYSDQGLNGMSQTYHELYRTRLAR 321 Query: 202 GEYRDEERPILVNNWEATYFDFNADKLAAIAEEGAKLGIELFVLDDGWVGE 252 G +RD ERPIL+NNWEATYF FN +KL IA+ A+LGIELFVLDDGW GE Sbjct: 322 GAFRDRERPILINNWEATYFHFNEEKLVNIAKTAAELGIELFVLDDGWFGE 372 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,743,066,430 Number of extensions: 74175759 Number of successful extensions: 142175 Number of sequences better than 1.0e-05: 429 Number of HSP's gapped: 141687 Number of HSP's successfully gapped: 429 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)