BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0013.p (480 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein 818 0.0 A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 pro... 306 3e-81 C5EMA4.1 C5EMA4_9FIRM Glycoside hydrolase 302 6e-80 C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase 300 2e-79 C4G1M0.1 C4G1M0_ABIDE Putative uncharacterized protein 286 4e-75 >B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein Length = 474 Score = 818 bits (2112), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/474 (79%), Positives = 422/474 (89%) Query: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYREGLDHGWHLLGTKDFVNYREDG 60 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYREGLDHGW+L+GTKDFVNYRE+G Sbjct: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYREGLDHGWYLVGTKDFVNYREEG 60 Query: 61 ACKIEGGTGHILKVDDVYHMFYCIFPEGKQFACHAVSKDLRTWEPIPEDTFGPDGEIYEL 120 CKIEGGTGHILKVD +YHMFYCIFP GKQ CHA+S+DL+TWEPIPEDTF D IYEL Sbjct: 61 PCKIEGGTGHILKVDGIYHMFYCIFPPGKQLVCHAISRDLKTWEPIPEDTFAADDRIYEL 120 Query: 121 ADWRDPFVFWNEEEGQYWMLIAALAKGPTNRKGCTGLLSSKDLKHWEYREPLYAPNLHVG 180 +DWRDPFVFWNEEEGQYWML++A+AKGPTNRKGCT LLSSKDLK WEYREPLYAPNLHVG Sbjct: 121 SDWRDPFVFWNEEEGQYWMLLSAMAKGPTNRKGCTALLSSKDLKAWEYREPLYAPNLHVG 180 Query: 181 AHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWITPPEEAFDGRAYYAAKSVSD 240 AHECPDLFR+GDWWYLIYSSYTGRF TFYRMSRSLNGPWITP E+ FDGRA+YAAKSV+D Sbjct: 181 AHECPDLFRIGDWWYLIYSSYTGRFATFYRMSRSLNGPWITPKEDTFDGRAFYAAKSVAD 240 Query: 241 GQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLVVHEIVQRPDGTLGVKAPETV 300 GQKRYLFGWNPTK DDLFGWNPPK+ GKDYDTWDWGGNLVVHEI+QRPDGTLGVK P+TV Sbjct: 241 GQKRYLFGWNPTKNDDLFGWNPPKSQGKDYDTWDWGGNLVVHEIIQRPDGTLGVKVPDTV 300 Query: 301 DSAFARQVPAHFYGVAGEWSITDEVIRCESPYSFAGCITQEELPDQCKISTTVRFSGSTQ 360 D+AF +P F V G W +D + CESPY FAGC T+E+LPDQCKIS +V FSG TQ Sbjct: 301 DAAFKELMPVRFNRVEGAWLDSDCSLSCESPYGFAGCTTREDLPDQCKISASVSFSGQTQ 360 Query: 361 GLGFMLRAGEGLDFAYYITLEPERSRIAFRGPIMQSEEGGKTFPYDMELERPLKLLPDQE 420 GLGFMLR + LD AYYIT EP+R+R+ FRG IMQSEEGGKTFPY++ELERP++L+P++ Sbjct: 361 GLGFMLRTEDTLDAAYYITFEPQRNRLTFRGYIMQSEEGGKTFPYEVELERPIELIPERP 420 Query: 421 YELKVFIDGTICEIYVGGDVAMSARMYDIQQGKLALFVSQGAAEFHGVKIEALG 474 YE+KVFIDG+ICEIYV ++AMSARMYDIQ GKL LFVSQG+A+F V+IE G Sbjct: 421 YEIKVFIDGSICEIYVDENIAMSARMYDIQHGKLGLFVSQGSAQFSDVRIEGRG 474 >A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 protein Length = 478 Score = 306 bits (784), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 179/495 (36%), Positives = 262/495 (52%), Gaps = 51/495 (10%) Query: 2 KEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYREGLDHGWHLLGTKDFVNYREDGA 61 + F++P + WVGD+IP+ +DGEF LFYLH R + + G W+L+ TKD + + G Sbjct: 3 RSVFFQPTHGWVGDLIPFEKDGEFWLFYLHEDRSDPKPGT--SWNLVTTKDLTQFEDHGV 60 Query: 62 CKIEGG---------TGHILK-VDDVYHMFYC------IFPEG--KQFACHAVSKD-LRT 102 G TG +L V+H+FY + P+G +Q HA S D +RT Sbjct: 61 SLSHGSESDLDFNAYTGSVLADAAGVHHLFYTGQNPRNVGPDGMPRQLVMHATSTDGMRT 120 Query: 103 WEPIPEDTFG-PDGEIYELADWRDPFVFWNEEEGQYWMLIAAL-AKGPTNRKGCTGLLSS 160 W+ PE TFG PDG YE DWRDPFVFW++ + Q+ ML+AA ++GP R+G S Sbjct: 121 WQKHPELTFGAPDG--YESGDWRDPFVFWDDSKNQWRMLLAARHSEGPERRRGVIAQCVS 178 Query: 161 KDLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWI 220 DL W++ EP + P ++ HECPD+F GDWWY++YS ++ F T YRM++S +GPW Sbjct: 179 TDLMTWQHTEPFWDPRRYI-THECPDVFSWGDWWYMVYSEFSESFTTRYRMAKSPDGPWT 237 Query: 221 TPPEEAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLV 280 P ++ DGRA+YA+K+ +R+ FGW +KE + D W W G + Sbjct: 238 VPALDSIDGRAFYASKTAERDGRRFFFGWIASKEGN-----------ADQGAWQWAGTMS 286 Query: 281 VHEIVQRPDGTLGVKAPETVDSAFARQVPAHFYGVAGEWSITDEVIRCESPYSFAGCITQ 340 V E Q PDGTLG + + +F V G R P + ++ Sbjct: 287 VLEARQNPDGTLGFSFADELVESFWDDVSVPLTGSLP--------ARLHVPDGYTALVSD 338 Query: 341 EELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIAF-RGPIMQSEEG 399 EELP Q + + T G +LR+ D +Y + LEP+R R+ F R P + + Sbjct: 339 EELPQQFYAKAVLDIAPDTTECGLLLRSSHDGDHSYVLRLEPKRGRLVFDRWPRTITGDA 398 Query: 400 ----GKTFPYDMELERPLKLLPDQEYELKVFIDGTICEIYVGGDVAMSARMYDIQQGKLA 455 P+D+ELERP L P E+ L+V +DG IC V VA+S R+YD+ G++ Sbjct: 399 QWHVSGDVPFDVELERPCDLTP-GEHTLEVIVDGDICVAVVDRQVALSTRIYDLTAGRIG 457 Query: 456 LFVSQGAAEFHGVKI 470 LF +G+A +++ Sbjct: 458 LFAGEGSATVTELEV 472 >C5EMA4.1 C5EMA4_9FIRM Glycoside hydrolase Length = 481 Score = 302 bits (773), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 179/500 (35%), Positives = 260/500 (52%), Gaps = 48/500 (9%) Query: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYRE-GLDHGWHLLGTKDFVNYRED 59 M + F++P+NAWVGD+IPYYEDG + +YLH R N E + WHL TKD ++ Sbjct: 1 MDKLFFKPDNAWVGDLIPYYEDGTYYAYYLHDPRVNPGEFAEETTWHLATTKDCRKFQYQ 60 Query: 60 GACKIEGG---------TGHILKV-DDVYHMFYCIFP-------EGKQFACHAVSKDLRT 102 G GG TG ++K ++ Y+ FY F + Q A S DL Sbjct: 61 GEAITRGGDDRPNKNIYTGSVIKKGENEYYAFYTAFNADIQIHGKSVQSVMRAFSTDLIH 120 Query: 103 WEPIPEDTFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAALAKGP-TNRKGCTGLLSSK 161 WE + F D +YE DWRDP+VF NEEEG YWML+AA KG +R GCT L S Sbjct: 121 WETDEDFLFVADDSMYESFDWRDPYVFKNEEEGNYWMLLAARKKGGGAHRGGCTALCKST 180 Query: 162 DLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWIT 221 DL +W Y EP YAP ++V ECP++F+MG WWYL++S+++ RF T YRM+R+L+GPW Sbjct: 181 DLVNWTYEEPFYAPEMYV-TMECPEVFQMGGWWYLVFSTFSDRFTTHYRMARTLDGPWGI 239 Query: 222 PPEEAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLVV 281 P ++ FD RA YA K+ DGQKR+ FGW + KA DY W+WGG +V Sbjct: 240 PDDDVFDTRADYAIKTAFDGQKRFAFGWIAS-----------KAGNNDYGPWEWGGTMVA 288 Query: 282 HEIVQRPDGTLGVKAPETVDSAFARQVPAH---FYGVAGEWSITDEVIRCESPYSFAGCI 338 HEIVQ PDGTL VK E + + + +P + +Y +I+ E+ + + Sbjct: 289 HEIVQEPDGTLSVKPTEAMKDFYNQVLPVNDLCYYHCTAREEAGKTIIQSETLGAVLFPV 348 Query: 339 TQEELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIAFRGPIMQSEE 398 ++ I+ G + G L G++ Y++ ++ R A+ M Sbjct: 349 PEQGFELDLTIT-----PGQSAETGVALHTDTGMENGYFLRMDLSRRTAAWD---MWPRS 400 Query: 399 GGKTFPYDM------ELERPLKLLPDQEYELKVFIDGTICEIYVGGDVAMSARMYDIQQG 452 G + + + ++E KL Y +++F + +C +YV +AMS RMYD + G Sbjct: 401 GQGVYQWQIKGDIPCQIETSRKLPAADTYHIRIFREDDLCVLYVNDRMAMSTRMYDHKGG 460 Query: 453 KLALFVSQGAAEFHGVKIEA 472 ++ G A G +I+ Sbjct: 461 LAGIYTVGGTAAVSGCQIKV 480 >C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase Length = 482 Score = 300 bits (768), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 185/506 (36%), Positives = 263/506 (51%), Gaps = 64/506 (12%) Query: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYRE---GLDHGWHLLGTKDFVNYR 57 MK FY+P + WVGD IP+ DG+F +FYLH R E G W+LL T+D VN + Sbjct: 1 MKNIFYKPGDGWVGDTIPFAHDGKFYIFYLHDERKGNTEDEYGYRTSWNLLITEDGVNIK 60 Query: 58 EDGACKIEGG---------TGHILKVDDV-YHMFYCI--------FPEGK--QFACHAVS 97 + GG TG +++ +D +HMFY G+ Q+ HA+S Sbjct: 61 DCKVVLPVGGYDDADYACYTGSVIEGNDSKFHMFYTAQNNYNPKYHRNGRPLQYVAHAIS 120 Query: 98 KDLRTWEPIPEDTFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAALAKGPTNRKG-CTG 156 +DL WE +PE TFG D IYE DWRDPFVF+NEEE + ML+AA +G + + G C G Sbjct: 121 EDLIHWEKLPELTFGADESIYEPFDWRDPFVFYNEEEECFDMLLAARLQGASEKNGGCVG 180 Query: 157 LLSSKDLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLN 216 L S DL HW+ +P Y P ++ HECPDLF+MGD WYLI+S+++ +F T YRM+ ++ Sbjct: 181 LCRSYDLIHWKAMQPFYNPQSYM-THECPDLFKMGDKWYLIFSTFSEKFVTHYRMADKIS 239 Query: 217 GPWITPPEEAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWG 276 GPW P E+ FD RA+YAAK+ R F W PTK D D+ ++WG Sbjct: 240 GPWTAPIEDTFDARAFYAAKTAQAKDVRMAFAWVPTKRGD-----------SDFGQYEWG 288 Query: 277 GNLVVHEIVQRPDGTLGVKAPETVDSAFARQVPAHFYGVAGEWSITDEVIRC---ESPYS 333 GNL+ HEI Q DG L VK + + F +++ A+ +I D I+ E Y Sbjct: 289 GNLIAHEIKQDKDGKLKVKPSNGLINIFEKEITAN--------NIEDVEIKNNEGEKVYV 340 Query: 334 FAGCITQEELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIA----- 388 G + + C I T+ F ++ G LR + YY+ EP +RI Sbjct: 341 IEG------MKETCLIEATIEFYEGSRAFGIGLRQDKDFANGYYLRFEPFYNRIVADMWP 394 Query: 389 --FRGPIMQSEEGGKTFPYDMELERPL--KLLPDQEYELKVFIDGTICEIYVGGDVAMSA 444 G +G K P+ +ELERP K L + + +KVF++G+I +YV VA++ Sbjct: 395 RRISGVNQWYVDGDK--PFMVELERPFDYKALEENKVNIKVFVEGSIICLYVNDTVALTM 452 Query: 445 RMYDIQQGKLALFVSQGAAEFHGVKI 470 R Y ++ FV G+ + + Sbjct: 453 RAYSTERKYWGFFVKDGSIRVCNIHM 478 >C4G1M0.1 C4G1M0_ABIDE Putative uncharacterized protein Length = 498 Score = 286 bits (731), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 181/494 (36%), Positives = 259/494 (52%), Gaps = 48/494 (9%) Query: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYRE-GLDHGWHLLGTKDFVNYRED 59 M + FYRPE AWVGD+IPYYEDG + FYLH R RE + WHL+ TKDFV Sbjct: 15 MGDLFYRPEQAWVGDLIPYYEDGIYYGFYLHDPRIRDREFAEETTWHLVTTKDFVKLTYK 74 Query: 60 GACKIEGG---------TGHILKV--DDVYHMFYCIFPE-----GK--QFACHAVSKDLR 101 G GG TG ++K D +YH+FY F E GK Q A K L Sbjct: 75 GEAIARGGDKEANKNAYTGSVIKCREDGLYHVFYTAFNEDFKIRGKAIQSVMQATGKTLD 134 Query: 102 TWEPIPEDTFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAALAKGP-TNRKGCTGLLSS 160 E + E F DGE YE DWRDP+VFWNE++G Y+ML+A+ G R GC L S Sbjct: 135 KLETVREFLFTSDGERYEEFDWRDPYVFWNEDDGCYYMLLASRMAGRGALRGGCIALCKS 194 Query: 161 KDLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWI 220 DL +W Y +P Y P +++ ECP+LF+MG++WYL++S+++ RF T YR+S++ NG W Sbjct: 195 YDLMNWTYEKPFYDPKMYI-TMECPELFKMGNYWYLVFSTFSDRFTTHYRISKNWNGSWE 253 Query: 221 TPPEEAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLV 280 P ++ FD RA YA K+ SDG++R+ FGW +K +G D+ W+WGGN+ Sbjct: 254 IPDDDVFDTRADYAIKTASDGKRRFAFGWIASK----YG-------NTDFGPWEWGGNMN 302 Query: 281 VHEIVQRPD-GTLGVKAPETVDSAFARQVPAHFYGVAGEWSITDEVIRCESPYSFAGCIT 339 HEIVQ P+ G L VKA + V F+ V V I + + G + Sbjct: 303 FHEIVQIPETGELRVKAVQGVLDYFSEDVKLESEEVYNG-RIENTGTGSKLTSDTLGALL 361 Query: 340 QEELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIAFRGPIMQSEEG 399 D ++ T+ G G L + ++ Y++ L+ +R +A+ + E Sbjct: 362 YPVKDDTFRLDMTI-IPGRQHEFGIALHVNKDMETGYFLRLDTKRRTVAW--DLWPRSEQ 418 Query: 400 GK-------TFPYDMELERPLKLLPD-QEYELKVFIDGTICEIYVGGDVAMSARMYDIQQ 451 G+ PY +E R LLP+ +EY + + + IC +Y+ ++A+S RMYD + Sbjct: 419 GRYQWQIKGDVPYQIETAR---LLPEAEEYRVHIIRETDICVVYINDEIALSTRMYDHKG 475 Query: 452 GKLALFVSQGAAEF 465 G ++V QG Sbjct: 476 GYAGIYVVQGEVNL 489 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.141 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,383,082,308 Number of extensions: 159372661 Number of successful extensions: 271911 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 271807 Number of HSP's successfully gapped: 59 Length of query: 480 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 340 Effective length of database: 1,741,754,103 Effective search space: 592196395020 Effective search space used: 592196395020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)