BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0010.p (528 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K657.1 B3K657_9BACI Glycosyl hydrolase family 32 domain protein 684 0.0 Q9LAL1.1 Q9LAL1_9PSED Exo-inulinase 593 e-167 Q8RLU2.1 Q8RLU2_PAEPO Exo-inulinase 509 e-142 B3KI94.1 B3KI94_9BACI Levanase EC=3.2.1.65 504 e-140 P05656.1 SACC_BACSU Levanase EC=3.2.1.80 480 e-133 >B3K657.1 B3K657_9BACI Glycosyl hydrolase family 32 domain protein Length = 821 Score = 684 bits (1765), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/525 (60%), Positives = 389/525 (74%), Gaps = 2/525 (0%) Query: 1 VTTRKGAIPAIPTYRQAAALVQSAGTDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYH 60 VTTRKGA+ ++PT + +++S E +RP H+SPP+ W+NDPNG+VY+EGEYH Sbjct: 296 VTTRKGALQSMPTIDEINKIMESH--KQMGEKFRPGIHFSPPSHWMNDPNGLVYYEGEYH 353 Query: 61 LMYQHHPESTVWGPMHWGHAVSKDLVNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFD 120 L YQHHP S WGPMHWGHAVS DL++W+ P+AL PD +GAIFSG VVD+ ++SGFF Sbjct: 354 LFYQHHPFSNKWGPMHWGHAVSHDLIHWQHEPIALFPDEHGAIFSGCCVVDFNNSSGFFA 413 Query: 121 GGSGLVAIFTHADIYPDSDRPRQRQSLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVF 180 G GLVAIFTHAD P++ +PRQRQSLAYS DKGR+W Y GNPVL++ + DFRDPKVF Sbjct: 414 GAHGLVAIFTHADTSPETGQPRQRQSLAYSSDKGRTWHKYEGNPVLTEPDLVDFRDPKVF 473 Query: 181 WMEVAGVWVMVLACGDHIRFYRSANLKDWDFASEFGKSEGSHYGVWECPDLFELSVDGSN 240 W + W+M + GDH+RFY S NL+DW F EFG+ EGSH GVWECPDLFEL D S Sbjct: 474 WHAQSERWIMTIVAGDHVRFYGSENLRDWSFTGEFGREEGSHDGVWECPDLFELPFDDSG 533 Query: 241 LKKWVLLVSIGDNPDYPEGSKTQYFIGSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSD 300 KWVL++SIGDNP PEGS+TQYFIG FDG F+N+N+PD ++WLD GRDNYA V+WSD Sbjct: 534 RSKWVLVISIGDNPSSPEGSRTQYFIGKFDGNTFINDNSPDHIMWLDHGRDNYAGVTWSD 593 Query: 301 IPEEDGRRVIIGWMSNWSYARQIPTGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQL 360 +PE+DGRRVIIGWMSNW YA + PTG+WRGAMTLPRVLSLT R+ +VL+QMPV EIEQL Sbjct: 594 LPEQDGRRVIIGWMSNWKYANETPTGAWRGAMTLPRVLSLTHRDEGIVLTQMPVGEIEQL 653 Query: 361 RKESLSWSDVSVTREAPFIHGTNXXXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPE 420 RKES W+ ++V E P++ RS EV IK++SSGQSET IGYDP Sbjct: 654 RKESWDWNMLTVAPEFPYVLKMKDDLLEIEADIDIRSAGEVYIKMQSSGQSETSIGYDPV 713 Query: 421 REWLFIDRSNSGLTDFNPSFACKHGARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQ 480 R+WLFIDRS SG+TDF+ SFACKHGARL P+NG I+LRIWLDR+ VEV+ANEGLVALTDQ Sbjct: 714 RQWLFIDRSKSGMTDFHASFACKHGARLVPDNGKIRLRIWLDRNAVEVYANEGLVALTDQ 773 Query: 481 IFPDEPIDTVWIGAETGKAEXXXXXXXXXXXXXXXXGSIGQISRR 525 IFPD PI+ + I E+G+ +IGQ S R Sbjct: 774 IFPDYPINELSISTESGQVVLNSLHVHALQSIHASNNNIGQTSGR 818 >Q9LAL1.1 Q9LAL1_9PSED Exo-inulinase Length = 501 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 274/474 (57%), Positives = 341/474 (71%), Gaps = 1/474 (0%) Query: 28 YSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVSKDLVN 87 Y +E +RPQFH++P W+NDPNG+VY+EGEYHL YQ+HP WGPMHWGHAVSKDL++ Sbjct: 5 YYQEKFRPQFHFTPETNWMNDPNGLVYYEGEYHLFYQYHPFGNTWGPMHWGHAVSKDLIH 64 Query: 88 WETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSGLVAIFTHADIYPDSDRPRQRQSL 147 W+ LP+AL PDH G IFSGSAV+DW+DTSGFF+GG+G+VA+FTHAD +PDS RPRQRQSL Sbjct: 65 WDHLPIALYPDHIGQIFSGSAVIDWQDTSGFFNGGAGMVALFTHADKFPDSGRPRQRQSL 124 Query: 148 AYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRFYRSANLK 207 AYS D+GR+W Y GNP +D +TDFRDPKVFW + + W+M+LA G+ +R Y S+NLK Sbjct: 125 AYSTDRGRTWIKYEGNPFFADEAMTDFRDPKVFWHKASNKWIMILAAGNCVRIYHSSNLK 184 Query: 208 DWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEGSKTQYFI 266 W FASEFG +EGSH GVWECPDLFEL VDG N KKWV++VSIG++ +Y EGS+TQYFI Sbjct: 185 QWKFASEFGANEGSHTGVWECPDLFELPVDGDRNRKKWVMVVSIGNSDEYLEGSRTQYFI 244 Query: 267 GSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSYARQIPTG 326 G FDG +F NEN P+TVLW+D GRDNYA V+WSDIPEEDGRR+ I WMSNW YA PT Sbjct: 245 GQFDGIRFTNENDPETVLWVDHGRDNYAGVTWSDIPEEDGRRLFIAWMSNWKYANHTPTE 304 Query: 327 SWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFIHGTNXXX 386 WR AMT+PR LSL S L Q PV E+E LR E L W D+ VT + + Sbjct: 305 IWRSAMTIPRSLSLRSTPEGTRLFQTPVTELESLRLEKLEWKDLEVTTNGNVLSNISGDI 364 Query: 387 XXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDFNPSFACKHGA 446 + E K+R S ETI+GYD E++ LFIDR+ SG++DF F CKHGA Sbjct: 365 FEIIAEFELNTALEFGFKVRKSADQETIVGYDAEQQMLFIDRTQSGVSDFCEHFPCKHGA 424 Query: 447 RLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAETGKAE 500 + P I++ I++DRS VEVF N G + +TD IFPD+ + + A+ G + Sbjct: 425 VMIPNQNRIQMHIFVDRSSVEVFGNNGELTMTDLIFPDDSSTGIEVYAKEGNVK 478 >Q8RLU2.1 Q8RLU2_PAEPO Exo-inulinase Length = 485 Score = 509 bits (1312), Expect = e-142, Method: Compositional matrix adjust. Identities = 254/456 (55%), Positives = 306/456 (67%), Gaps = 3/456 (0%) Query: 30 KESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVSKDLVNWE 89 +E YRP +H+SP + W+NDPNGMVY+EG YHL YQHHP T WGPMHWGHAVS DL+NWE Sbjct: 6 QEKYRPSYHFSPKSGWMNDPNGMVYYEGRYHLFYQHHPFGTTWGPMHWGHAVSTDLMNWE 65 Query: 90 TLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSGLVAIFTHADIYPDSDRPRQRQSLAY 149 P+AL PD G IFSGSAVVD +DTSGFF G GLVAIFTH P +++ RQ QSLAY Sbjct: 66 EQPIALEPDELGTIFSGSAVVDEQDTSGFFGGKPGLVAIFTHHASLPGTEQVRQCQSLAY 125 Query: 150 SKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRFYRSANLKDW 209 S D G++W Y GNPVL D DFRDPKVFW + WVMVLACG IR Y S NLK+W Sbjct: 126 STDSGKTWIKYTGNPVLEDKECIDFRDPKVFWHKPTEQWVMVLACGQTIRIYHSPNLKEW 185 Query: 210 DFASEFGKSEGSHYGVWECPDLFELSVDGSNLK-KWVLLVSIGDNPDYPEGSKTQYFIGS 268 FASEFG GSH VWECPDLF L +D + KWV+LVSIGD P+ EGS+TQYF G Sbjct: 186 TFASEFGHGIGSHDAVWECPDLFPLYIDEKREQVKWVMLVSIGDVPEIREGSRTQYFTGE 245 Query: 269 FDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSYARQIPTGSW 328 FDG FV + + V WLD GRDNYA V WSDIP EDGRR+ +GWMSNW YA Q PT W Sbjct: 246 FDGTTFVADAESEKVRWLDYGRDNYAGVCWSDIPAEDGRRLFMGWMSNWRYANQTPTERW 305 Query: 329 RGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFIHGTNXXXXX 388 RGAM++PR L+L +R GTV L Q PV+E+E LR LS ++ S + Sbjct: 306 RGAMSIPRELALETRKGTVALVQRPVRELEGLRTPVLSLTEPSWEEVRNALSALQLDCYE 365 Query: 389 XXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDFNPSFACKHGARL 448 +GD K+R S + ET++GY + +++DR SG +DF+ FA KHGA L Sbjct: 366 LVAEFAT-TGD-FGFKVRVSDEQETLVGYTSATQEVYVDRKRSGRSDFHEDFAGKHGASL 423 Query: 449 APENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPD 484 + +RI++DRS +EVF + G VA+TD IFPD Sbjct: 424 TILPDRMDIRIYVDRSSIEVFFDRGQVAITDLIFPD 459 >B3KI94.1 B3KI94_9BACI Levanase EC=3.2.1.65 Length = 491 Score = 504 bits (1298), Expect = e-140, Method: Compositional matrix adjust. Identities = 247/460 (53%), Positives = 313/460 (68%), Gaps = 4/460 (0%) Query: 26 TDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVSKDL 85 T+ +++YR +H+SP W+NDPNGMVYF GEYHL YQHHP T WGPMHWGHAVS+DL Sbjct: 2 TNTEQQNYRNAYHFSPQKNWMNDPNGMVYFNGEYHLFYQHHPFGTTWGPMHWGHAVSRDL 61 Query: 86 VNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSGLVAIFTHADIYPDSDRPRQRQ 145 V+WE LP+AL PD +G IFSGSAVVDW ++SGFFDGG GLVAIFTH + + Q Q Sbjct: 62 VSWEELPIALAPDEHGTIFSGSAVVDWNNSSGFFDGGPGLVAIFTHHHDVQGAQQMIQYQ 121 Query: 146 SLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRFYRSAN 205 SLAYSKD+GR+W+ Y GNPVLS DFRDPKVFW E + WVM++ACG + YRS+N Sbjct: 122 SLAYSKDEGRTWTKYDGNPVLSHDSYVDFRDPKVFWHEPSSQWVMIVACGQTVCLYRSSN 181 Query: 206 LKDWDFASEFGKSEGSHYGVWECPDLFELSVDGSNLKKWVLLVSIGDNPDYPEGSKTQYF 265 LK+W FASEFG+ G H GVWECPDLF L+ + + +WV+LVSIGD+P Y EGS+TQYF Sbjct: 182 LKEWVFASEFGEGIGFHDGVWECPDLFPLAAEDGEV-RWVMLVSIGDDPAYVEGSRTQYF 240 Query: 266 IGSFDGWKFV-NENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSYARQIP 324 IG FDG F+ +E + V WLD GRDNYA VSWSDIP DGRR+ IGWMSNW YA P Sbjct: 241 IGEFDGTTFMPDERSKGVVRWLDYGRDNYAGVSWSDIPAADGRRLFIGWMSNWKYANLTP 300 Query: 325 TGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFIHGTNX 384 T WRGAMTL R L L + G V ++Q P +E+E R E LS ++V A + Sbjct: 301 TDGWRGAMTLVRELKLETLEGEVTVTQQPARELEAHRTELLSLHGIAVNEAAVRLAAVRL 360 Query: 385 XXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDFNPSFACKH 444 + + +++R+ ET++G+D E +++DRS SG DF+ F KH Sbjct: 361 ESCEILVRFD--AAFSLGLRVRTGLGQETLVGWDAESAEVYVDRSRSGQADFHKDFPGKH 418 Query: 445 GARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPD 484 A+L ++LRI++DRS VE+FA+ G +TD I+PD Sbjct: 419 SAQLKTSGSMLELRIFVDRSSVELFADRGQAVITDLIYPD 458 >P05656.1 SACC_BACSU Levanase EC=3.2.1.80 Length = 677 Score = 480 bits (1236), Expect = e-133, Method: Compositional matrix adjust. Identities = 253/482 (52%), Positives = 306/482 (63%), Gaps = 15/482 (3%) Query: 23 SAGTDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVS 82 +A + Y E YRPQ+H++P A W+NDPNGMVY+ GEYHL YQ+HP WGPMHWGHAVS Sbjct: 24 AADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVS 83 Query: 83 KDLVNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSG-LVAIFTHADIYPDSDRP 141 KDLV WE LPVAL PD G IFSGSAVVD +TSGF G LVAI+T DR Sbjct: 84 KDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQ-------DRE 136 Query: 142 -RQRQSLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRF 200 Q QS+AYS DKGR+W+ YAGNPV+ + G DFRDPKVFW E WVMVLA GD I Sbjct: 137 GHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILI 196 Query: 201 YRSANLKDWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEG 259 Y S NLK W +ASEFG+ +GSH GVWECPDLFEL VDG+ N KKWV+ VS+G N G Sbjct: 197 YTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGG 255 Query: 260 SKTQYFIGSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSY 319 S QYF+G FDG F NEN P+ VLW D GRD YAAVSWSDIP D RR+ +GWMSNW Y Sbjct: 256 SGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQY 315 Query: 320 ARQIPTGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFI 379 A +PT WR A ++PR L L + V + Q PV+E+E +R S W +++++ + + Sbjct: 316 ANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNV 375 Query: 380 ---HGTNXXXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDF 436 + S E K+R+ T +GYD LF+DRS SG F Sbjct: 376 LAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTF 435 Query: 437 NPSFAC-KHGARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAE 495 NP+F K A L P NG +KLRI++DRS VEVF N+G +TD I PD + + A Sbjct: 436 NPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAA 495 Query: 496 TG 497 G Sbjct: 496 NG 497 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,479,488,994 Number of extensions: 156491247 Number of successful extensions: 277496 Number of sequences better than 1.0e-05: 1248 Number of HSP's gapped: 273203 Number of HSP's successfully gapped: 1315 Length of query: 528 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 387 Effective length of database: 1,732,333,088 Effective search space: 670412905056 Effective search space used: 670412905056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)