BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0009.p (317 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0K6E1.1 B0K6E1_THEPX PfkB domain protein 278 6e-73 B0K7P7.1 B0K7P7_THEP3 PfkB domain protein 277 9e-73 B3K657.1 B3K657_9BACI Glycosyl hydrolase family 32 domain protein 267 9e-70 B7XWH1.1 B7XWH1_RUMHA Putative uncharacterized protein 261 8e-68 C0ZKJ4.1 C0ZKJ4_BREBN Probable fructokinase EC=2.7.1.4 257 1e-66 >B0K6E1.1 B0K6E1_THEPX PfkB domain protein Length = 322 Score = 278 bits (710), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 138/250 (55%), Positives = 182/250 (72%), Gaps = 3/250 (1%) Query: 25 MVDITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQF 84 M D+ A+GE+LIDFTPAG S+ G LFE NPGGAPANV+ + KLG AFIGKVG+DQF Sbjct: 1 MYDVIALGELLIDFTPAGFSDNGNTLFEMNPGGAPANVLTAVTKLGGKGAFIGKVGDDQF 60 Query: 85 GEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDL 144 G +L ++L N +NT GL ++A TTLAFVHL + GDRSF+FYRNPGAD +L +DI+L Sbjct: 61 GYFLKKVLENNQINTDGLKFTTKANTTLAFVHLDDKGDRSFTFYRNPGADTMLEEEDIEL 120 Query: 145 EQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRI 204 + I+ KIFHFGS+SMT EPS SATLKA +AK+N +IS+DPN RP LW++ AK + Sbjct: 121 DLIEKGKIFHFGSLSMTDEPSRSATLKAIEYAKQNKKIISYDPNWRPPLWENETVAKKEM 180 Query: 205 KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFGPGSK 264 GL ADIVK+SE+EL+F+TG ++LE G++ + +K+VLVT KGC+ R G+ Sbjct: 181 VLGLQYADIVKLSEDELQFLTGESNLEYGSKTL-FDMGIKLVLVTLGAKGCYYRHTSGT- 238 Query: 265 WADVPGYKVD 274 +P Y+V+ Sbjct: 239 -GHIPAYRVN 247 >B0K7P7.1 B0K7P7_THEP3 PfkB domain protein Length = 322 Score = 277 bits (709), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 138/250 (55%), Positives = 182/250 (72%), Gaps = 3/250 (1%) Query: 25 MVDITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQF 84 M D+ A+GE+LIDFTPAG S+ G LFE NPGGAPANV+ + KLG AFIGKVG+DQF Sbjct: 1 MCDVIALGELLIDFTPAGFSDNGNTLFEMNPGGAPANVLTAVTKLGGKGAFIGKVGDDQF 60 Query: 85 GEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDL 144 G +L ++L N +NT GL ++A TTLAFVHL + GDRSF+FYRNPGAD +L +DI+L Sbjct: 61 GYFLKKVLENNQINTDGLKFTTKANTTLAFVHLDDKGDRSFTFYRNPGADTMLEEEDIEL 120 Query: 145 EQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRI 204 + I+ KIFHFGS+SMT EPS SATLKA +AK+N +IS+DPN RP LW++ AK + Sbjct: 121 DLIEKGKIFHFGSLSMTDEPSRSATLKAIEYAKQNKKIISYDPNWRPPLWENETVAKKEM 180 Query: 205 KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFGPGSK 264 GL ADIVK+SE+EL+F+TG ++LE G++ + +K+VLVT KGC+ R G+ Sbjct: 181 VLGLQYADIVKLSEDELQFLTGESNLEYGSKTL-FDMGIKLVLVTLGAKGCYYRHTSGT- 238 Query: 265 WADVPGYKVD 274 +P Y+V+ Sbjct: 239 -GHIPAYRVN 247 >B3K657.1 B3K657_9BACI Glycosyl hydrolase family 32 domain protein Length = 821 Score = 267 bits (683), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 133/250 (53%), Positives = 172/250 (68%), Gaps = 2/250 (0%) Query: 25 MVDITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQF 84 M+D+ AIGE+LIDFTP G S G + ECNPGGAPANV L++LG A I KVG+D F Sbjct: 1 MLDVIAIGELLIDFTPVGRSVGGNEQLECNPGGAPANVAAALSRLGAKAALISKVGKDHF 60 Query: 85 GEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDL 144 G +L LL V+ L AKTTLAFVHL + GDRSFSFYR PGAD LL +D+ L Sbjct: 61 GSFLHNTLLSCEVDVSALSFTDEAKTTLAFVHLDDSGDRSFSFYRQPGADTLLRSEDVPL 120 Query: 145 EQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRI 204 ++I ++ HFGS+SMTHEP+ SAT A + A+E G L+SFDPN+R +LW E+AK I Sbjct: 121 DRIGNCQVLHFGSLSMTHEPARSATRAAVVKAQEAGVLLSFDPNIRFSLWGSKEEAKQNI 180 Query: 205 KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFGPGSK 264 G+ ADI+KISEEEL FITG+TD+E+G+ +Q QF + ++VT E+GC+ R Sbjct: 181 LWGMKHADILKISEEELCFITGITDVEKGSLMLQQQFGIAFIVVTIGEQGCYYRVAAHEG 240 Query: 265 WADVPGYKVD 274 + VPG+KV+ Sbjct: 241 Y--VPGFKVN 248 >B7XWH1.1 B7XWH1_RUMHA Putative uncharacterized protein Length = 319 Score = 261 bits (666), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 126/230 (54%), Positives = 170/230 (73%) Query: 25 MVDITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQF 84 M D+ A+GE+LIDFT +G SE+G QL E NPGGAP NV+ +L ++GK TAFIGKVG+D F Sbjct: 1 MYDVVALGELLIDFTESGKSEQGNQLLEVNPGGAPCNVLAMLKQMGKKTAFIGKVGQDMF 60 Query: 85 GEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDL 144 G L + L E G+++K L+ + +TTLAFVH DGDR FSFYRNPGADM+L ++I+ Sbjct: 61 GNLLKQTLDEVGIDSKNLIMDEEVRTTLAFVHTLADGDREFSFYRNPGADMMLSEEEINP 120 Query: 145 EQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRI 204 E IK TKIFHFG++SMTHE + +AT KA AKE GALISFDPNLR LW+ LE AK ++ Sbjct: 121 EVIKDTKIFHFGTLSMTHEGARNATKKAVSIAKEAGALISFDPNLREPLWESLELAKEQM 180 Query: 205 KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKG 254 + G DI+KIS+ E++F+TG D +EG +Q ++++ ++L+TK + G Sbjct: 181 EYGFRQCDILKISDNEIQFVTGKEDYDEGIAILQEKYQIPLILLTKGKDG 230 >C0ZKJ4.1 C0ZKJ4_BREBN Probable fructokinase EC=2.7.1.4 Length = 321 Score = 257 bits (656), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 131/250 (52%), Positives = 171/250 (68%), Gaps = 3/250 (1%) Query: 25 MVDITAIGEVLIDFTPAGLSEK-GEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQ 83 MVD+ A GE+LIDFTP EK G FE NPGGAPANV+ L++ GK T+FIG VG D Sbjct: 1 MVDVVACGELLIDFTPVQQKEKPGSIAFEQNPGGAPANVLAALSRFGKRTSFIGAVGNDV 60 Query: 84 FGEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDID 143 FG +L + L+ + TKGLV A TTLAFVHL E GDRSF FYRNPGAD++L D++ Sbjct: 61 FGRFLQQTLIRLNIGTKGLVLTEEAHTTLAFVHLDETGDRSFHFYRNPGADIMLREQDVN 120 Query: 144 LEQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIR 203 I IFHFG++S+THEP+ SAT +A +AK++ L+SFDPN+R +LW+DLE+AK Sbjct: 121 EALIAQAAIFHFGTLSLTHEPARSATWRAVEYAKQHQRLLSFDPNIRASLWEDLEEAKAL 180 Query: 204 IKEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFGPGS 263 +G+A ADIVK+SEEEL F+ G D+ E T + Q+ ++ V VT EKGCF R + Sbjct: 181 ALKGMAKADIVKLSEEELAFLVGSEDVVEATAWMLGQYDLQAVFVTLGEKGCFYR--TRN 238 Query: 264 KWADVPGYKV 273 + V G++V Sbjct: 239 NFGTVGGFQV 248 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,917,051,186 Number of extensions: 80777909 Number of successful extensions: 175929 Number of sequences better than 1.0e-05: 2738 Number of HSP's gapped: 174079 Number of HSP's successfully gapped: 2739 Length of query: 317 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 182 Effective length of database: 1,788,859,178 Effective search space: 325572370396 Effective search space used: 325572370396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)