BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0001.p (1129 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q1A2D0.1 Q1A2D0_PAEPO Cel44C 533 e-149 B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 447 e-123 B8DYX7.1 B8DYX7_DICTD Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 446 e-123 B2AEP0.1 B2AEP0_PODAN Predicted CDS Pa_5_1950 442 e-122 O30654.1 O30654_DICTH Beta-mannanase EC=3.2.1.78 424 e-116 >Q1A2D0.1 Q1A2D0_PAEPO Cel44C Length = 1352 Score = 533 bits (1373), Expect = e-149, Method: Compositional matrix adjust. Identities = 266/495 (53%), Positives = 340/495 (68%), Gaps = 21/495 (4%) Query: 54 VSKVLLNAGNNSIKVTRGWGYYGIEHIKLEPAKDPSAGNIL--------------EAEDG 99 V+ L+ VT G YY + + K S NIL EAE+G Sbjct: 604 VASNLVETSYTDTNVTNGTSYY--YKVTAKTNKGSSESNILKAVPKTPVNGPARYEAEEG 661 Query: 100 MMSGGVSVGTAGDGYSGEGYVI-FQQSG-SLTLTYKTPLAGTYDILVGYCAPNGEKKTSM 157 + G + V ++G GYSG GYV F G SLT+T + P AG Y++ +GY +P+ +K+T+ Sbjct: 662 ALKGTI-VESSGTGYSGTGYVTNFHNPGDSLTMTIQAPTAGLYNLTIGYRSPHDDKRTNF 720 Query: 158 VLNGHT-SEITLPETTGFIEISAGKTWMNQGDNTIEILANWGWYNIDYVKLTAAAKPEQR 216 LNG E+ L +TT F E S GK +N G NTI WGWY+IDYV+L AA Sbjct: 721 SLNGKAFGELLLKKTTDFKETSGGKVLLNAGANTIGFETGWGWYDIDYVRLEPAADRPPH 780 Query: 217 EVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEWIGQETGKYPAILATDLMD 276 V TL NP+AT EA+ALMN++ QYG+ ++SGQ+ + + +W+ GK PAI A DL+D Sbjct: 781 AVTKTLTNPNATVEAKALMNYLVDQYGKNMLSGQEEINEIDWLQANVGKKPAIAALDLID 840 Query: 277 YSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGGEEPGYEWWRGFYTEYTTF 336 YSPSR E+G SSTE EK IAW +GGIV+ WHWNAPKG+ + G EWWRGFY + TTF Sbjct: 841 YSPSRAEHGLSSTEAEKAIAWDKQGGIVTFAWHWNAPKGLIDTQ-GKEWWRGFYADSTTF 899 Query: 337 DVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRPLHEAEGGWFWWGAKGPEP 396 D+EYA++HP+SEDY LLIRDID IA QLK+LQ+A VPVL+RPLHEAEG WFWWGAKGPEP Sbjct: 900 DIEYAMNHPESEDYKLLIRDIDVIAGQLKKLQDAKVPVLFRPLHEAEGKWFWWGAKGPEP 959 Query: 397 AKRLWKLMVDRLTSHHHLNNLIWVWNSEKPEWYPGDDVVDIASVDIYNPAGDYNPSIAKY 456 K+L+ LM DRLT+ H LNNLIWVWNS P+WYPGD+ VDI S D Y AGDY+P IAKY Sbjct: 960 VKKLYILMHDRLTNVHKLNNLIWVWNSVAPDWYPGDEYVDILSFDSYPQAGDYSPQIAKY 1019 Query: 457 ENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFTTWTGSFIKDGATNSAEHLQRIY 516 E+LV+L KK++ ++ENGPIPDP++++AY A WS+F TW G F++DG NS EHL+++Y Sbjct: 1020 EDLVALGKDKKLVAMSENGPIPDPDLMKAYQAHWSWFATWYGDFVRDGKQNSLEHLKKVY 1079 Query: 517 KHDYVVTLDELPADL 531 H V+TLDELP +L Sbjct: 1080 NHPNVITLDELPTNL 1094 Score = 64.7 bits (156), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 37/87 (42%), Positives = 49/87 (56%), Gaps = 5/87 (5%) Query: 1 VNVAEAGLYKMSIGYYIPEGYGGKATGIQVNGIGTGELMLDAPAAGTVRAEKMVSKVLLN 60 + AGLY ++IGY P + K T +NG GEL+L T E KVLLN Sbjct: 695 IQAPTAGLYNLTIGYRSP--HDDKRTNFSLNGKAFGELLLKKT---TDFKETSGGKVLLN 749 Query: 61 AGNNSIKVTRGWGYYGIEHIKLEPAKD 87 AG N+I GWG+Y I++++LEPA D Sbjct: 750 AGANTIGFETGWGWYDIDYVRLEPAAD 776 Score = 62.0 bits (149), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 38/117 (32%), Positives = 59/117 (50%), Gaps = 1/117 (0%) Query: 536 KYEAEKGELTGLEVSDEQGAYSGSGYITGMDAQGGRLRIKVDA-SPGTYSLMIRYKTGNV 594 +YEAE+G L G V YSG+GY+T G L + + A + G Y+L I Y++ + Sbjct: 655 RYEAEEGALKGTIVESSGTGYSGTGYVTNFHNPGDSLTMTIQAPTAGLYNLTIGYRSPHD 714 Query: 595 QTKNSISVNGASAVEYTFADAAEWTDTVTGSYPLQKGENTIDIISAEKGTDIDYIKL 651 + + S+NG + E ++ +T G L G NTI + DIDY++L Sbjct: 715 DKRTNFSLNGKAFGELLLKKTTDFKETSGGKVLLNAGANTIGFETGWGWYDIDYVRL 771 >B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 469 Score = 447 bits (1150), Expect = e-123, Method: Compositional matrix adjust. Identities = 216/442 (48%), Positives = 298/442 (67%), Gaps = 11/442 (2%) Query: 94 LEAEDGMMSGGVSVGTAGDGYSGEGYVI-FQQSG-SLTLTYKTPLAGTYDILVGYCAPNG 151 +EAE+G+++G V GY G GYV F + G S ++T++ +G Y++++GY AP G Sbjct: 26 IEAENGVLNG-TYVARQFPGYQGTGYVDGFDKDGDSCSVTFEVKESGMYELIIGYAAPYG 84 Query: 152 EKKTSMVLNGH-TSEITLPETTGFIEISAGKTWMNQGDNTIEILANWGWYNIDYVKLTAA 210 K+ S+ +NG + + P++ F + AG + G NTI I+ +WGW+ +DY K+ A Sbjct: 85 YKENSLYVNGEFQTNVKFPQSQKFTTVYAGLIPLKNGKNTISIVKSWGWFLLDYFKIKKA 144 Query: 211 AKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEWIGQETGKYPAIL 270 P + L+ P+ + EA+ LM+++ S YG+ +SGQ +DA WI T K+PAI Sbjct: 145 EIPTMNPT-NKLVTPNPSKEAQKLMDYLVSIYGKYTLSGQMGYKDAFWIWNITDKFPAIC 203 Query: 271 ATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGGEEPGYEWWRGFY 330 D+MDYSPSRVE GASS ++E I W+N GGIV WHWNAPKG+ + PG EWWRGFY Sbjct: 204 GFDMMDYSPSRVERGASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLY-DTPGKEWWRGFY 262 Query: 331 TEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRPLHEAEGGWFWWG 390 T T+FD+EYA +HP+SEDY L+IRDID IA+QLKRLQ A VP+LWRPLHEAEG WFWWG Sbjct: 263 TNATSFDIEYAFNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWG 322 Query: 391 AKGPEPAKRLWKLMVDRLTSHHHLNNLIWVW-NSEKPE---WYPGDDVVDIASVDIYNPA 446 AKGPE K+LW+L+ DRL ++H +NNLIWVW ++ P+ WYPGD+ VDI DIY Sbjct: 323 AKGPEACKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDALKWYPGDEYVDIVGADIYLKD 382 Query: 447 GDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFTTWTGSFIKDGAT 506 DY+PS + N+V L GKK++ L ENG IPDP++++ A W +F TW+G F D Sbjct: 383 KDYSPSTGMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFMTWSG-FENDPNK 441 Query: 507 NSAEHLQRIYKHDYVVTLDELP 528 N H+++++ H +V+T DELP Sbjct: 442 NEISHIKKVFNHPFVITKDELP 463 Score = 64.7 bits (156), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 37/117 (31%), Positives = 56/117 (47%), Gaps = 1/117 (0%) Query: 538 EAEKGELTGLEVSDEQGAYSGSGYITGMDAQGGRLRIKVDAS-PGTYSLMIRYKTGNVQT 596 EAE G L G V+ + Y G+GY+ G D G + + G Y L+I Y Sbjct: 27 EAENGVLNGTYVARQFPGYQGTGYVDGFDKDGDSCSVTFEVKESGMYELIIGYAAPYGYK 86 Query: 597 KNSISVNGASAVEYTFADAAEWTDTVTGSYPLQKGENTIDIISAEKGTDIDYIKLTR 653 +NS+ VNG F + ++T G PL+ G+NTI I+ + +DY K+ + Sbjct: 87 ENSLYVNGEFQTNVKFPQSQKFTTVYAGLIPLKNGKNTISIVKSWGWFLLDYFKIKK 143 >B8DYX7.1 B8DYX7_DICTD Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 469 Score = 446 bits (1147), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/443 (48%), Positives = 300/443 (67%), Gaps = 11/443 (2%) Query: 93 ILEAEDGMMSGGVSVGTAGDGYSGEGYVI-FQQSG-SLTLTYKTPLAGTYDILVGYCAPN 150 ++EAE+G+++G V GY G GYV F + G S T+T++ AG Y++++GY AP Sbjct: 25 VVEAENGVLNG-TYVAKNLPGYQGTGYVDGFDRDGDSCTITFEVKEAGMYELIIGYAAPY 83 Query: 151 GEKKTSMVLNG-HTSEITLPETTGFIEISAGKTWMNQGDNTIEILANWGWYNIDYVKLTA 209 G K+ S+ +NG + + P + F + G + G NTI I+ +WGW+ +DY K+ Sbjct: 84 GYKENSLYVNGVFQTNVKFPPSQSFTTVYGGLIPLKSGKNTISIVKSWGWFLLDYFKIKK 143 Query: 210 AAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEWIGQETGKYPAI 269 A P + L+ P+ + EA+ LM+++ S YG+ +SGQ +DA WI T K+PAI Sbjct: 144 AELPTMNPT-NKLVTPNPSKEAQKLMDYLVSIYGKYTLSGQMGYKDAFWIWNITDKFPAI 202 Query: 270 LATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGGEEPGYEWWRGF 329 D++DYSPSRVE GASS ++E I W+N GGIV WHWNAPKG+ + PG EWWRGF Sbjct: 203 CGFDMIDYSPSRVERGASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLY-DTPGKEWWRGF 261 Query: 330 YTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRPLHEAEGGWFWW 389 YT T+FD+EYAL+HP+SEDY L+IRDID IA+QLKRLQ A VP+LWRPLHEAEG WFWW Sbjct: 262 YTNATSFDIEYALNHPESEDYKLIIRDIDAIAVQLKRLQEARVPILWRPLHEAEGRWFWW 321 Query: 390 GAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVW-NSEKPE---WYPGDDVVDIASVDIYNP 445 GAKGPEP K+LW+L+ DRL ++H +NNLIWVW ++ P+ WYPGD+ VDI D+Y Sbjct: 322 GAKGPEPCKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDALKWYPGDEYVDIVGADVYLN 381 Query: 446 AGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFTTWTGSFIKDGA 505 +Y+PS + N+V + GKK++ L ENG IPDP++++ A W++F TW+G F D Sbjct: 382 DKNYSPSTGMFYNIVKIFGGKKLVALTENGIIPDPDLMKEQKAYWAWFMTWSG-FENDPN 440 Query: 506 TNSAEHLQRIYKHDYVVTLDELP 528 N H+++++ H +V+T DELP Sbjct: 441 KNEISHIKKVFNHPFVITKDELP 463 Score = 63.5 bits (153), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 38/117 (32%), Positives = 54/117 (46%), Gaps = 1/117 (0%) Query: 538 EAEKGELTGLEVSDEQGAYSGSGYITGMDAQGGRLRIKVDAS-PGTYSLMIRYKTGNVQT 596 EAE G L G V+ Y G+GY+ G D G I + G Y L+I Y Sbjct: 27 EAENGVLNGTYVAKNLPGYQGTGYVDGFDRDGDSCTITFEVKEAGMYELIIGYAAPYGYK 86 Query: 597 KNSISVNGASAVEYTFADAAEWTDTVTGSYPLQKGENTIDIISAEKGTDIDYIKLTR 653 +NS+ VNG F + +T G PL+ G+NTI I+ + +DY K+ + Sbjct: 87 ENSLYVNGVFQTNVKFPPSQSFTTVYGGLIPLKSGKNTISIVKSWGWFLLDYFKIKK 143 >B2AEP0.1 B2AEP0_PODAN Predicted CDS Pa_5_1950 Length = 469 Score = 442 bits (1138), Expect = e-122, Method: Compositional matrix adjust. Identities = 229/448 (51%), Positives = 293/448 (65%), Gaps = 7/448 (1%) Query: 83 EPAKDPSAGNILEAEDGMMSGGVSVGTAGDGYSGEGYVIFQQSGSLTLTYKTPLAGT--Y 140 +P K EAED +++G +V TA GY+G GYV GS +T++ A T Y Sbjct: 21 KPCKPRDGPVTYEAEDAILTG-TTVDTAQVGYTGRGYVTGFDEGSDKITFQISSATTKLY 79 Query: 141 DILVGYCAPNGEKKTSMVLN-GHTSEITLPETTGFIEISAGKTWMNQGDNTIEILANWGW 199 D+ + Y A G+K+T++VLN G SE+ P F ++AG+ +N G NTI+I+ NWGW Sbjct: 80 DLSIRYAAIYGDKRTNVVLNNGAVSEVFFPAGDSFTSVAAGQVLLNAGQNTIDIVNNWGW 139 Query: 200 YNIDYVKLTAAAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEWI 259 Y ID + LT +A ++ L NP+A A+ L +++ S YG KIISGQQ L AEWI Sbjct: 140 YLIDSITLTPSAPRPPHDINPNLNNPNADTNAKKLYSYLRSVYGNKIISGQQELHHAEWI 199 Query: 260 GQETGKYPAILATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGGE 319 Q+TGK PA++A DLMDYSPSRVE G +S +E IA +N GGIVS+ WHWNAP G+ Sbjct: 200 RQQTGKTPALVAVDLMDYSPSRVERGTTSHAVEDAIAHHNAGGIVSVLWHWNAPVGLYDT 259 Query: 320 EPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRPL 379 E +WW GFYT T FD+ L +P +Y LLIRDID IA+QLKRL+ AGVPVLWRPL Sbjct: 260 EEN-KWWSGFYTRATDFDIAATLANPQGANYTLLIRDIDAIAVQLKRLEAAGVPVLWRPL 318 Query: 380 HEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVWNSEKPEWYPGDDVVDIAS 439 HEAEGGWFWWGAKGPEPAK+LW ++ +RLT HH L+NLIWVWNS +WYPGDD VDI S Sbjct: 319 HEAEGGWFWWGAKGPEPAKQLWDILYERLTVHHGLDNLIWVWNSILEDWYPGDDTVDILS 378 Query: 440 VDIYNPAGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFTTWTGS 499 D+Y A P +Y L++L KK+I AE G P P +LQAY A+W +F W Sbjct: 379 ADVY--AQGNGPMSTQYNELIALGRDKKMIAAAEVGAAPLPGLLQAYQANWLWFAVWGDD 436 Query: 500 FIKDGATNSAEHLQRIYKHDYVVTLDEL 527 FI + + N+ L IY DYV+TLDE+ Sbjct: 437 FINNPSWNTVAVLNEIYNSDYVLTLDEI 464 Score = 63.2 bits (152), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 40/123 (32%), Positives = 57/123 (46%), Gaps = 1/123 (0%) Query: 535 LKYEAEKGELTGLEVSDEQGAYSGSGYITGMDAQGGRLRIKV-DASPGTYSLMIRYKTGN 593 + YEAE LTG V Q Y+G GY+TG D ++ ++ A+ Y L IRY Sbjct: 30 VTYEAEDAILTGTTVDTAQVGYTGRGYVTGFDEGSDKITFQISSATTKLYDLSIRYAAIY 89 Query: 594 VQTKNSISVNGASAVEYTFADAAEWTDTVTGSYPLQKGENTIDIISAEKGTDIDYIKLTR 653 + ++ +N + E F +T G L G+NTIDI++ ID I LT Sbjct: 90 GDKRTNVVLNNGAVSEVFFPAGDSFTSVAAGQVLLNAGQNTIDIVNNWGWYLIDSITLTP 149 Query: 654 LTP 656 P Sbjct: 150 SAP 152 >O30654.1 O30654_DICTH Beta-mannanase EC=3.2.1.78 Length = 398 Score = 424 bits (1089), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/394 (50%), Positives = 271/394 (68%), Gaps = 8/394 (2%) Query: 140 YDILVGYCAPNGEKKTSMVLNGH-TSEITLPETTGFIEISAGKTWMNQGDNTIEILANWG 198 +++++GY AP G K+ S+ +NG + + P++ F + AG + G NTI I+ +WG Sbjct: 2 HELIIGYAAPYGYKENSLYVNGEFQTNVKFPQSQKFTTVYAGLIPLKNGKNTISIVKSWG 61 Query: 199 WYNIDYVKLTAAAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEW 258 W+ +DY K+ A P + L+ P+ + EA+ LM+++ S YG+ +SGQ +DA W Sbjct: 62 WFLLDYFKIKKAEIPTMNPT-NKLVTPNPSKEAQKLMDYLVSIYGKYTLSGQMGYKDAFW 120 Query: 259 IGQETGKYPAILATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGG 318 I T K+PAI D+MDYSPSRVE GASS ++E I W+N GGIV WHWNAPKG+ Sbjct: 121 IWNITDKFPAICGFDMMDYSPSRVERGASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLY- 179 Query: 319 EEPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRP 378 + PG EWWRGFYT T+FD+EYAL+HP+SEDY L+IRDID IA+QLKRLQ A VP+LWRP Sbjct: 180 DTPGKEWWRGFYTNATSFDIEYALNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRP 239 Query: 379 LHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVW-NSEKPE---WYPGDDV 434 LHEAEG WFWWGAKGPE K+LW+L+ DRL ++H +NNLIWVW ++ P+ WYPGD+ Sbjct: 240 LHEAEGRWFWWGAKGPEACKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDALKWYPGDEY 299 Query: 435 VDIASVDIYNPAGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFT 494 VDI DIY DY+PS + N+V L GKK++ L ENG IPDP++++ A W +F Sbjct: 300 VDIVGADIYLKDKDYSPSTGMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFM 359 Query: 495 TWTGSFIKDGATNSAEHLQRIYKHDYVVTLDELP 528 TW+G F D N H+++++ H +V+T DELP Sbjct: 360 TWSG-FENDPNKNEISHIKKVFNHPFVITKDELP 392 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 6,913,919,405 Number of extensions: 311153795 Number of successful extensions: 766308 Number of sequences better than 1.0e-05: 1575 Number of HSP's gapped: 762083 Number of HSP's successfully gapped: 1774 Length of query: 1129 Length of database: 3,060,696,203 Length adjustment: 147 Effective length of query: 982 Effective length of database: 1,675,806,998 Effective search space: 1645642472036 Effective search space used: 1645642472036 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 137 (57.4 bits)