BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0594.0002.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2UMK9.1 B2UMK9_AKKM8 Cytidyltransferase-related domain protein 417 e-115 C0VEW9.1 C0VEW9_9MICO Cytidyltransferase-related enzyme 390 e-106 C0WD98.1 C0WD98_9FIRM Glycerol-3-phosphate cytidyltransferase 386 e-105 A5UNE1.1 A5UNE1_METS3 Glycerol-3-phosphate cytidyltransferase, T... 355 4e-96 C4FMD3.1 C4FMD3_9FIRM Putative uncharacterized protein 355 6e-96 >B2UMK9.1 B2UMK9_AKKM8 Cytidyltransferase-related domain protein Length = 451 Score = 417 bits (1073), Expect = e-115, Method: Compositional matrix adjust. Identities = 217/430 (50%), Positives = 278/430 (64%), Gaps = 3/430 (0%) Query: 1 LFHEGHYRLLQRAKELGDYLIVGVTTEGYDQTRGKLNVIDSLMTRVENVRKTGFADEIII 60 L H GH LL+RA++LGD LIVGVTT+ YDQ+RGKLNV++SL R+ENVRKTG AD II Sbjct: 12 LLHTGHVNLLKRARKLGDRLIVGVTTDSYDQSRGKLNVMESLEERMENVRKTGLADLIIK 71 Query: 61 EESQGQKFRDIKKYQIDIFTVGSDWTGQFDYLNDYCKVVYLERTKDISSTMLRIKNKHXX 120 EE +GQK DI+KY D+F +GSDW+G+FDYL DYC+VVYLERTK +SST LR Sbjct: 72 EELEGQKIHDIRKYGADVFVIGSDWSGKFDYLRDYCEVVYLERTKGVSSTDLRSARNPIV 131 Query: 121 XXXXXXXXXXXXXFIPEVKLVSGINTQGIYNPNAESAIRFAE-KWQINPYTDIGDFFAAV 179 F+ E K VS I ++ N E RFAE + YT+ F V Sbjct: 132 YMGIAGHGRIAGRFLRESKYVSNIEITAVFGRNEEKVRRFAEFHALLEYYTEYEQFLDRV 191 Query: 180 DAVYIASPHETHYKYIKMSLEHGKHVLCEKPMVLEKTQAEELFGYAKEHNLVLFEGIKTA 239 AVYIA PH HY+ + +L GKHVLCEKP+ L + +AEELF A+E +VL E +KTA Sbjct: 192 HAVYIAVPHHLHYEMARRALLRGKHVLCEKPLALAREEAEELFRLAEEKGVVLLEALKTA 251 Query: 240 YCPGFSKLMGIACSGMIGSIRNVEACFTKL-ENPASRELTDLKFGGSFTELGSYTILPII 298 +CP F +L +A SG+IGSI+ V+A FTKL E+ A+RE ++ GG++TELGSY I Sbjct: 252 FCPAFQQLTSLAGSGIIGSIKAVDATFTKLIEDEAAREYDPMQAGGAWTELGSYPAFVIG 311 Query: 299 KIFGTEYEDIRF-DLIKDKNGLDIFTKAYFRYPNGLGTATCGLGVKSEGQLIISGTKGYI 357 K+ GTE IRF K G+D+FT+A F Y N + TAT +G K EG L I+GT+GYI Sbjct: 312 KLLGTEPRRIRFVTCRKPHTGVDVFTRAEFLYSNAVATATAAIGAKQEGDLCITGTEGYI 371 Query: 358 KVDSPWWKTTYFEVHYENQNDVEKYSDRFLGDGLRYEINDFLSMINGNTESEFKLTSKDS 417 V +PWWKT FEV +E+ KY F GDGLRYE+ FL +I+G LT +DS Sbjct: 372 YVPAPWWKTEMFEVRFEDARLNRKYFANFEGDGLRYELGAFLRLIHGCQHGNRLLTREDS 431 Query: 418 IAIAEIMERF 427 + +A++ RF Sbjct: 432 VFMADVASRF 441 >C0VEW9.1 C0VEW9_9MICO Cytidyltransferase-related enzyme Length = 447 Score = 390 bits (1003), Expect = e-106, Method: Compositional matrix adjust. Identities = 196/427 (45%), Positives = 280/427 (65%), Gaps = 4/427 (0%) Query: 1 LFHEGHYRLLQRAKELGDYLIVGVTTEGYDQTRGKLNVIDSLMTRVENVRKTGFADEIII 60 LFH+GH R+L+RA+ELGD+L+VGVT++ +D++RGKL+V S++ R+ENVR++G ADEII+ Sbjct: 12 LFHDGHRRILERARELGDHLVVGVTSDAFDESRGKLDVTQSVVERIENVRRSGLADEIIV 71 Query: 61 EESQGQKFRDIKKYQIDIFTVGSDWTGQFDYLNDYCKVVYLERTKDISSTMLRIKNKHXX 120 EE QGQK RDI + IDIFTVGSDW G+FDYL +YC+VVYLERT+ +SST LR + Sbjct: 72 EEYQGQKIRDIVERDIDIFTVGSDWIGKFDYLGEYCEVVYLERTRGVSSTELRAEGLGIQ 131 Query: 121 XXXXXXXXXXXXXFIPEVKLVSGINTQGIYNPNAESAIRFAEKWQINPYTD-IGDFFAAV 179 F+ E + VSG+ +++PN A F +++ + D D A V Sbjct: 132 RIGIVGTGRIARRFVAEARYVSGVTVDSVFSPNHAVAKDFVDEFDLRRAADSFDDLIADV 191 Query: 180 DAVYIASPHETHYKYIKMSLEHGKHVLCEKPMVLEKTQAEELFGYAKEHNLVLFEGIKTA 239 DAVYIASPH TH Y + +++ G HVLCEKP+ L A L+ A+ +VL E +KTA Sbjct: 192 DAVYIASPHGTHVDYTRRAIDAGVHVLCEKPVALRADDAAALYAEARARGVVLLEALKTA 251 Query: 240 YCPGFSKLMGIACSGMIGSIRNVEACFTKLENPASRELTDLKFGGSFTELGSYTILPIIK 299 PGF +L+ +A SG IG+IR+V+A FTKL + RE GG+ +EL SY +L I+K Sbjct: 252 QAPGFRRLITLARSGTIGTIRSVDATFTKLVD-GGREY-QAPDGGAISELASYNLLAIVK 309 Query: 300 IFGTEYEDIRF-DLIKDKNGLDIFTKAYFRYPNGLGTATCGLGVKSEGQLIISGTKGYIK 358 + G++Y D+R L+ +G++ F + Y + + G+G KSEG L+++G KGYI Sbjct: 310 LLGSDYVDLRATKLVPGDDGVETFARIDLTYAHATASCRVGIGAKSEGSLVVTGEKGYIY 369 Query: 359 VDSPWWKTTYFEVHYENQNDVEKYSDRFLGDGLRYEINDFLSMINGNTESEFKLTSKDSI 418 V +PWW T+YFE +E+ ++ RF GDGLRYEI++FL +I + F LTS +S+ Sbjct: 370 VPAPWWLTSYFEERFEDSRRKRRHYYRFDGDGLRYEISEFLHLIRQSRSESFHLTSDESV 429 Query: 419 AIAEIME 425 AIA ++E Sbjct: 430 AIARMIE 436 >C0WD98.1 C0WD98_9FIRM Glycerol-3-phosphate cytidyltransferase Length = 445 Score = 386 bits (992), Expect = e-105, Method: Compositional matrix adjust. Identities = 202/427 (47%), Positives = 269/427 (62%), Gaps = 6/427 (1%) Query: 1 LFHEGHYRLLQRAKELGDYLIVGVTTEGYDQTRGKLNVIDSLMTRVENVRKTGFADEIII 60 LFH+GHY LL+RAKELGDYLIVGVT++ +D++RGK NV DSLMTR+ENV+ TGFAD+I++ Sbjct: 12 LFHQGHYNLLKRAKELGDYLIVGVTSDYFDKSRGKFNVHDSLMTRIENVKATGFADQIVV 71 Query: 61 EESQGQKFRDIKKYQIDIFTVGSDWTGQFDYLNDYCKVVYLERTKDISSTMLRIKNKHXX 120 EE GQK DIKKY +DIFTVGSDW G FDYLN+YCKVVYLERTK ISST +I+N H Sbjct: 72 EEYFGQKIDDIKKYDVDIFTVGSDWKGHFDYLNEYCKVVYLERTKGISST--QIRNSHSI 129 Query: 121 XXXXXXXXXXXXXFIPEVKLVSGINTQGIY--NPNAESAIRFAEKWQINPYTDIGDFFAA 178 F+ E+ VSGI G++ + + + + + Y+ +A Sbjct: 130 RLGIIGDEQILDRFLAELNYVSGIEPVGVFAADKDVSAVYKSTVAKEFTQYSTTDSLLSA 189 Query: 179 VDAVYIASPHETHYKYIKMSLEHGKHVLCEKPMVLEKTQAEELFGYAKEHNLVLFEGIKT 238 VDAVYI P + +YIK +L GKHVL E P A+EL A + LVL EG+KT Sbjct: 190 VDAVYINVPLKVRPQYIKAALLQGKHVLTEFPFSSNYESAKELIDLALQKKLVLNEGLKT 249 Query: 239 AYCPGFSKLMGIACSGMIGSIRNVEACFTKLENPASRELTDLKFGGSFTELGSYTILPII 298 AYCP F KL+ + SG IG + ++EA FT++ R + GGS LG+Y +L I Sbjct: 250 AYCPAFGKLISLVKSGQIGKVVSIEANFTQILGDNLRNQIRIA-GGSMLSLGAYPLLAIF 308 Query: 299 KIFGTEYEDIRFDLIKDKNGLDIFTKAYFRYPNGLGTATCGLGVKSEGQLIISGTKGYIK 358 K+ G +++D +F + +N +D T+ YP+ + TA + K+EG L+ISGTKGY+ Sbjct: 309 KLLGHDFKDAQF-ITHLENQIDTITRVTLTYPHAMATALVAINAKAEGDLVISGTKGYVY 367 Query: 359 VDSPWWKTTYFEVHYENQNDVEKYSDRFLGDGLRYEINDFLSMINGNTESEFKLTSKDSI 418 V +PWWKT Y+EV YEN N KY +F G+GLRYEI +F+ I LT D + Sbjct: 368 VPAPWWKTEYYEVRYENINRNAKYFYKFEGEGLRYEIVEFVRNIQDEQFDNLLLTKADML 427 Query: 419 AIAEIME 425 A A++ME Sbjct: 428 AEAKVME 434 >A5UNE1.1 A5UNE1_METS3 Glycerol-3-phosphate cytidyltransferase, TagD EC=2.7.7.39 Length = 438 Score = 355 bits (911), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 192/429 (44%), Positives = 265/429 (61%), Gaps = 12/429 (2%) Query: 1 LFHEGHYRLLQRAKELGDYLIVGVTTEGYDQTRGKLNVIDSLMTRVENVRKTGFADEIII 60 LFH GH RLL+RAK LGDYLIVGVT + +D+ RGK+NV SLM R+E+VR TG ADEIII Sbjct: 12 LFHYGHQRLLERAKALGDYLIVGVTADDFDKNRGKINVQQSLMERIESVRATGLADEIII 71 Query: 61 EESQGQKFRDIKKYQIDIFTVGSDWTGQFDYLNDYCKVVYLERTKDISSTMLRIKNKHXX 120 EE +GQK DIK+Y IDIFTVGSDW G+FDYL +YC VVYLERT+ +SST +R K++ Sbjct: 72 EEYEGQKIDDIKRYDIDIFTVGSDWVGEFDYLREYCDVVYLERTEGVSSTDIRSKDRQ-V 130 Query: 121 XXXXXXXXXXXXXFIPEVKLVSGINTQGIYNPNAESAIRFAEKWQINPYTDIGDFFAAVD 180 F E + V+G+ GI + + F + + D + VD Sbjct: 131 TLGLVGEGLVLNKFYNESQYVNGLTVGGICSGDENQLKDFDDDLILTQ--DFDELLNNVD 188 Query: 181 AVYIASPHETHYKYIKMSLEHGKHVLCEKPMVLEKTQAEELFGYAKEHNLVLFEGIKTAY 240 A++I S HYK K +LE+G HVLCE P+ L++ + ELF AK++NL+L + IKTAY Sbjct: 189 AIFIVSHPSKHYKQAKKALENGVHVLCESPIALKEEELCELFKLAKKNNLILMDSIKTAY 248 Query: 241 CPGFSKLMGIACSGMIGSIRNVEACFTKLENPASRELTDLKFGGSFTELGSYTILPIIKI 300 +++L+ +A G IG + +V+A T L + + + S T G +LP+ ++ Sbjct: 249 STAYNRLLLLAKKGKIGDVISVDATCTSLADGSGDDWN------SITAWGPTALLPVFQL 302 Query: 301 FGTEYEDIRFD--LIKDKNGLDIFTKAYFRYPNGLGTATCGLGVKSEGQLIISGTKGYIK 358 GT+Y+D + L+ D+FTK F YPN + + G GVKSEG+LIISGTKGYI Sbjct: 303 LGTDYKDKNINSLLLDSAENFDLFTKIDFTYPNAVASIKVGKGVKSEGELIISGTKGYIY 362 Query: 359 VDSPWWKTTYFEVHYENQNDVEKYSDRFLGDGLRYEINDFLSMINGNTESEFKLTSKDSI 418 V +PWWKT YFE+ +ENQN+ +KY + G+G+RYE+ F+ I N + + S Sbjct: 363 VPAPWWKTDYFEIRFENQNENKKYFYQLDGEGIRYELVAFIKSIELNKTGSY-IDINISK 421 Query: 419 AIAEIMERF 427 I+ +ME F Sbjct: 422 EISSVMEDF 430 >C4FMD3.1 C4FMD3_9FIRM Putative uncharacterized protein Length = 444 Score = 355 bits (910), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 201/437 (45%), Positives = 270/437 (61%), Gaps = 17/437 (3%) Query: 1 LFHEGHYRLLQRAKELGDYLIVGVTTEGYDQTRGKLNVIDSLMTRVENVRKTGFADEIII 60 LFHEGHY LL+ AK LGDYLIVGVT++ +D++RGK NV DSLMTR++NVR TGFADEI++ Sbjct: 12 LFHEGHYNLLKNAKALGDYLIVGVTSDYFDKSRGKFNVRDSLMTRIDNVRATGFADEIVV 71 Query: 61 EESQGQKFRDIKKYQIDIFTVGSDWTGQFDYLNDYCKVVYLERTKDISSTMLRIKNKHXX 120 EE GQK DIK++ +DIFTVGSDW G FDYLN+YCKVVYL RT+ ISST +I+N Sbjct: 72 EEYFGQKIDDIKRFNVDIFTVGSDWEGYFDYLNEYCKVVYLPRTQGISST--QIRNSENV 129 Query: 121 XXXXXXXXXXXXXFIPEVKLVSGINTQGIYNPNAESAIRFAEKWQINPYTDIGDFFAA-- 178 F+ E K VSGI+ G Y E+ + + +Q N D+ F ++ Sbjct: 130 RLGIIGNEVILDRFLDESKFVSGIDIIGGYTEADETDTIY-KSYQFN---DLEQFCSSEK 185 Query: 179 ----VDAVYIASPHETHYKYIKMSLEHGKHVLCEKPMVLEKTQAEELFGYAKEHNLVLFE 234 VDAVYI P YI+ +L+ KHVL E P + + L AK NLVL E Sbjct: 186 LLDEVDAVYINMPLSKRGAYIEKALQARKHVLTEFPFSSDLDETLRLMELAKSSNLVLME 245 Query: 235 GIKTAYCPGFSKLMGIACSGMIGSIRNVEACFTKLENPASRELTDLKFGGSFTELGSYTI 294 G+KTAY P F+KL+ +A SG IG I NVEA FT++ + GGS L SY + Sbjct: 246 GLKTAYSPAFNKLIAMARSGRIGKIFNVEANFTQVLGDDLANQVRIA-GGSILSLASYPL 304 Query: 295 LPIIKIFGTEYEDIRFDLIKDK-NGLDIFTKAYFRYPNGLGTATCGLGVKSEGQLIISGT 353 L I K+ G Y R D I K + +DI +K F Y + + +AT + K+EG L+ISG+ Sbjct: 305 LAIFKLLGFNYN--RVDFISHKVDDVDILSKINFLYDDAMASATVAINAKAEGDLVISGS 362 Query: 354 KGYIKVDSPWWKTTYFEVHYENQNDVEKYSDRFLGDGLRYEINDFLSMINGNTESEFKLT 413 KGYI V +PWWKT +FE+ +E+ N +KY +F G+GLRY I +FL I ++ES F ++ Sbjct: 363 KGYIYVPAPWWKTEFFEMRFEDVNLNQKYFYKFEGEGLRYGIVEFLKCIREDSES-FLMS 421 Query: 414 SKDSIAIAEIMERFLQE 430 +D I ++ +F+ + Sbjct: 422 HQDMIEEVRVLNKFVSD 438 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,490,487,028 Number of extensions: 105933814 Number of successful extensions: 232028 Number of sequences better than 1.0e-05: 2115 Number of HSP's gapped: 231626 Number of HSP's successfully gapped: 2116 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)