BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0034.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4VZE0.1 A4VZE0_STRS2 Lipase 243 2e-62 A4VT54.1 A4VT54_STRSY Lipase 243 2e-62 B1C458.1 B1C458_9FIRM Putative uncharacterized protein 189 3e-46 C0ZWF6.1 C0ZWF6_RHOE4 Putative hydrolase 105 6e-21 B3KF04.1 B3KF04_9BACI Lipase 104 1e-20 >A4VZE0.1 A4VZE0_STRS2 Lipase Length = 321 Score = 243 bits (619), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 121/276 (43%), Positives = 176/276 (63%), Gaps = 9/276 (3%) Query: 53 SVPENYTTTVKTAGELEAKYMAMGSHEVSYFESYAMMSFQKYEIFYPSDIADIDKQPPVV 112 SV NYT V+T G +E KY+ G+++VSY E A+ +F+KYE++YP++I ++ PVV Sbjct: 41 SVASNYTEVVETGGAVEKKYLGQGNYDVSYLEINALQNFKKYELYYPTNIETETRKFPVV 100 Query: 113 VFVNGTGIKGSKYKALQKHMASWGFITIATEEDFAWNGFSAEMSVRFLEKLNSSELFDN- 171 +F NGTG++ SKY ++ KH+ASWGFI I TEE+++WNGFS+EMS+R KLN ++ + Sbjct: 101 IFSNGTGVRASKYASVLKHLASWGFIVIGTEEEYSWNGFSSEMSLRLAIKLNDNKTVEGL 160 Query: 172 KDNVFYQKINLNNIGITGHSQGGYGVVNAITVQKHAASYKAAVILSSDASFSGNDFMWDA 231 K N F+ K++L+ IG++GHSQGG GV NA+T QKH K S ++ WD Sbjct: 161 KSNPFFSKVDLDKIGLSGHSQGGVGVFNAVTEQKHGHMIKTIYAASPANLELSSNLEWDY 220 Query: 232 DSTLIKTPSLIV-GSTGEFDSVVASLENLQGLYSNIXXXXXXXXDGTTKVLARRNDADHG 290 D +LI P+ +V G+ G ++ +V S + L +Y + TKV+ARR +A HG Sbjct: 221 DPSLISVPTFLVSGTGGGYEDLVVSGKQLTQIYETLPAT-------ITKVMARRKEAGHG 273 Query: 291 QMLYYADGYVTAWFMYYLQDDEEAKAAFFTNDAEIL 326 ML +GY+ AWFM+ LQ EA+ AF N EI+ Sbjct: 274 DMLRLPNGYMVAWFMWRLQGAPEAETAFQGNSPEIV 309 >A4VT54.1 A4VT54_STRSY Lipase Length = 321 Score = 243 bits (619), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 121/276 (43%), Positives = 176/276 (63%), Gaps = 9/276 (3%) Query: 53 SVPENYTTTVKTAGELEAKYMAMGSHEVSYFESYAMMSFQKYEIFYPSDIADIDKQPPVV 112 SV NYT V+T G +E KY+ G+++VSY E A+ +F+KYE++YP++I ++ PVV Sbjct: 41 SVASNYTEVVETGGAVEKKYLGQGNYDVSYLEINALQNFKKYELYYPTNIETETRKFPVV 100 Query: 113 VFVNGTGIKGSKYKALQKHMASWGFITIATEEDFAWNGFSAEMSVRFLEKLNSSELFDN- 171 +F NGTG++ SKY ++ KH+ASWGFI I TEE+++WNGFS+EMS+R KLN ++ + Sbjct: 101 IFSNGTGVRASKYASVLKHLASWGFIVIGTEEEYSWNGFSSEMSLRLAIKLNDNKTVEGL 160 Query: 172 KDNVFYQKINLNNIGITGHSQGGYGVVNAITVQKHAASYKAAVILSSDASFSGNDFMWDA 231 K N F+ K++L+ IG++GHSQGG GV NA+T QKH K S ++ WD Sbjct: 161 KSNPFFSKVDLDKIGLSGHSQGGVGVFNAVTEQKHGHMIKTIYAASPANLELSSNLEWDY 220 Query: 232 DSTLIKTPSLIV-GSTGEFDSVVASLENLQGLYSNIXXXXXXXXDGTTKVLARRNDADHG 290 D +LI P+ +V G+ G ++ +V S + L +Y + TKV+ARR +A HG Sbjct: 221 DPSLISVPTFLVSGTGGGYEDLVVSGKQLTQIYETLPAT-------ITKVMARRKEAGHG 273 Query: 291 QMLYYADGYVTAWFMYYLQDDEEAKAAFFTNDAEIL 326 ML +GY+ AWFM+ LQ EA+ AF N EI+ Sbjct: 274 DMLRLPNGYMVAWFMWRLQGAPEAETAFQGNSPEIV 309 >B1C458.1 B1C458_9FIRM Putative uncharacterized protein Length = 310 Score = 189 bits (480), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/278 (36%), Positives = 163/278 (58%), Gaps = 16/278 (5%) Query: 51 IQSVPENYTTTVKTAGELEAKYMAMGSHEVSYFESYAMMSFQKYEIFYPSDIADIDKQPP 110 + +VP Y + V+T G +E KY+ MGS+ +++ + Y I+YP ++ DK+ P Sbjct: 29 VPTVPNKYISKVETGGIIEEKYLKMGSYAINHTTFKGSELTKLYTIYYPKELEIKDKEYP 88 Query: 111 VVVFVNGTGIKGSKYKALQKHMASWGFITIATEEDFAWNGFSAEMSVRFLEKLNSSELFD 170 V+ +NGTG+ K KAL +H++SW FI + ++ NG S++ ++ F+ LN S Sbjct: 89 VIFVLNGTGVLPKKCKALFEHLSSWKFIVVGNDDPSTGNGKSSDETMDFIFSLNES---- 144 Query: 171 NKDNVFYQKINLNNIGITGHSQGGYGVVNAITVQKHAASYKAAVILSSDASFSGNDFMWD 230 K ++FY +I+L NIGI GHSQGG GV +A++ K+ YK V LS + F W+ Sbjct: 145 -KGSIFYHQIDLENIGIIGHSQGGAGVFSALSFSKYKDYYKTGVSLSPTHEEVAHAFGWN 203 Query: 231 ADSTLIKTPSLI-VGSTGEFDS-VVASLENLQGLYSNIXXXXXXXXDGTTKVLARRNDAD 288 D + I P ++ G+ G+F++ +V L+ + +Y I ++K++ARR + Sbjct: 204 YDLSKINVPIIMFAGTKGDFETKLVIPLDKMIKMYDKIP---------SSKIMARRIGVE 254 Query: 289 HGQMLYYADGYVTAWFMYYLQDDEEAKAAFFTNDAEIL 326 HGQMLY DGY+TAWFM+ LQ+D++A AF +E L Sbjct: 255 HGQMLYSVDGYITAWFMWQLQEDKKASNAFLGEKSEFL 292 >C0ZWF6.1 C0ZWF6_RHOE4 Putative hydrolase Length = 296 Score = 105 bits (262), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 91/313 (29%), Positives = 143/313 (45%), Gaps = 55/313 (17%) Query: 31 LAVVGAGSLLLNGCGKVME---NIQSVPENYTTTVKTAGELEAKYMAMGSHEVSYFESYA 87 +AVV A S +L G V + + PE TT + + Y A G+HEV A Sbjct: 9 IAVVTA-SFVLALSGAVTHASAGVPAAPEAATTESIASESISDLYAAQGAHEV------A 61 Query: 88 MMSFQKYEIFYPSDIADIDKQPPVVVFVNGTGIKGSKYKALQKHMASWGFITIATEEDFA 147 S Y++FYP+D++ K+ PV+ F NG+ +Y+AL +H ASWGF+ + + Sbjct: 62 ADSVPGYQLFYPADLSADGKRHPVITFGNGSYATFEEYEALLRHFASWGFVVVVADSSVT 121 Query: 148 WNGFSAEMSVRFLEKLNSSELFDNKDNVFYQKINLNNIGITGHSQGGYGVVNAITVQKHA 207 +G + ++L N+ N +++F+Q+I+ +NI GHSQG G +NA T Sbjct: 122 GDGTEILGAAQYLVDQNT-----NAESIFHQRIDTDNIASVGHSQGAGGAINAAT----- 171 Query: 208 ASYKAAVILSSDASFSGNDFMWDADSTLIKTPSLIVGS----TGEFDSVVASLENLQGLY 263 ++A ++++ A D W + + + + G TG D V S + Q Y Sbjct: 172 ---QSANLITATAVLDLPDPWWASRPVDVVDVAKLTGPTFFMTGADDPV--STADPQAEY 226 Query: 264 SNIXXXXXXXXDGTTKVLARRNDADHGQML-----YYAD-----GYVTAWFMYYLQDDEE 313 D T R G++L + AD GY+TAW + L D+ Sbjct: 227 ----------FDKTAAPAVR------GRLLGVGHNWAADGEGFRGYLTAWLRFALSGDQT 270 Query: 314 AKAAFFTNDAEIL 326 A AF + AEIL Sbjct: 271 AGRAFVGDSAEIL 283 >B3KF04.1 B3KF04_9BACI Lipase Length = 309 Score = 104 bits (259), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 78/265 (29%), Positives = 121/265 (45%), Gaps = 22/265 (8%) Query: 66 GELEAKYMAMGSHEVSYFESYAMMSFQKYEIFYPSDIADIDKQPPVVVFVNGTGIKGSKY 125 G +E +Y GS+ V E M + I+YP+ D+ P++ + NGT +Y Sbjct: 42 GSIEQRYAQKGSYHVKVEEVKRMSGEALFRIYYPA--FQEDESYPIISWGNGTDATPDRY 99 Query: 126 KALQKHMASWGFITIATEEDFAWNGFSAEMSVRFLEKLNSSELFDNKDNVFYQKINLNNI 185 L H+ASWGFI I + G ++ +L+ N + +++FYQKI + +I Sbjct: 100 DELLTHLASWGFIVIDSYSKTTGTGKEIVEAIDYLKNEN-----EQANSLFYQKIQMEHI 154 Query: 186 GITGHSQGGYGVVNAITVQKHAASYKAAV-ILSSDASFSGNDFMWDADSTLIKTPSLIVG 244 G+ GHSQG GV+NA T K + K V I D + ++ D+ I P LI+G Sbjct: 155 GVAGHSQGATGVINAHTNYKSGSLIKTVVSIALPDLKHCDPEDVY--DTARITVPFLIMG 212 Query: 245 STGEFDSVVASLENLQGLYSNIXXXXXX---XXDGTTKVLARRNDADHGQMLYYADGYVT 301 T +F +++ + Q N G N +H GY+T Sbjct: 213 GTRDF--IISPVSTNQLALHNTHASTPVMMGMAKGAAHTAIEGNGGNH-------RGYLT 263 Query: 302 AWFMYYLQDDEEAKAAFFTNDAEIL 326 AW Y L DD+EA AF + +E++ Sbjct: 264 AWMRYRLFDDQEAMKAFHGDSSEMM 288 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,787,514,079 Number of extensions: 69889903 Number of successful extensions: 224836 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 224839 Number of HSP's successfully gapped: 15 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)