BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0030.p (214 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q4ZSH7.1 Q4ZSH7_PSEU2 Isochorismatase hydrolase 312 1e-83 Q881B5.1 Q881B5_PSESM Isochorismatase family protein 308 2e-82 Q48J46.1 Q48J46_PSE14 Isochorismatase family protein 301 2e-80 C1V9L4.1 C1V9L4_9EURY Nicotinamidase-like amidase 119 1e-25 Q3IT58.1 Q3IT58_NATPD Probable isochorismatase 2; probable nicot... 119 3e-25 >Q4ZSH7.1 Q4ZSH7_PSEU2 Isochorismatase hydrolase Length = 212 Score = 312 bits (799), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 145/209 (69%), Positives = 173/209 (82%) Query: 2 KAKMKWDIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYL 61 K ++W I P RTAVIVVDMQ VFC+P GALYV T I+ I+ L ARAA + VVYL Sbjct: 3 KPLVRWPINPLRTAVIVVDMQKVFCEPSGALYVKSTADIVQPIQMLLQTARAARVMVVYL 62 Query: 62 RHIVRGDGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDL 121 RHIVRGDGSDTGRM D+YPNV++IL R PGVE+I+ LAP P D+IVDKLFYSGFH+TDL Sbjct: 63 RHIVRGDGSDTGRMRDLYPNVDQILARHDPGVEVIEALAPRPGDVIVDKLFYSGFHNTDL 122 Query: 122 DTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAE 181 DT+LR D++TII+CGTVTNVCCETT+RDG HREYKVI LSDANAAMDYPD+G+G +SAE Sbjct: 123 DTVLRARDVDTIIVCGTVTNVCCETTIRDGVHREYKVIALSDANAAMDYPDVGFGAVSAE 182 Query: 182 EIQRVTLTVVAYEFGQVSLTEDIIQEIQS 210 E+QR++LT +AYEFG+V+ T D+IQ I S Sbjct: 183 EVQRISLTTIAYEFGEVTTTADVIQRIAS 211 >Q881B5.1 Q881B5_PSESM Isochorismatase family protein Length = 227 Score = 308 bits (788), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 143/208 (68%), Positives = 172/208 (82%) Query: 2 KAKMKWDIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYL 61 K ++W I P RTAVIVVDMQ VFC+P GALYV T I+ ++ L AARAA + VVYL Sbjct: 18 KPLVQWPINPRRTAVIVVDMQKVFCEPHGALYVKSTADIVRPVQTLLCAARAAQVMVVYL 77 Query: 62 RHIVRGDGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDL 121 RH VRGDGSDTGRM D+YPNV++IL R P VEIID LAPE D+I+DKLFYSGFH+TDL Sbjct: 78 RHTVRGDGSDTGRMRDLYPNVDQILARHDPDVEIIDALAPESGDVIIDKLFYSGFHNTDL 137 Query: 122 DTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAE 181 DT+LR D++TIIICGTVTNVCCETT+RDG HREYKVI LSDANAAMDYPD+G+G +SAE Sbjct: 138 DTVLRARDVDTIIICGTVTNVCCETTIRDGVHREYKVIALSDANAAMDYPDVGFGAVSAE 197 Query: 182 EIQRVTLTVVAYEFGQVSLTEDIIQEIQ 209 E+QR++LT +AYEFG+V+ T D+I+ I+ Sbjct: 198 EVQRISLTTIAYEFGEVTTTADVIRRIE 225 >Q48J46.1 Q48J46_PSE14 Isochorismatase family protein Length = 252 Score = 301 bits (772), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 139/209 (66%), Positives = 174/209 (83%) Query: 2 KAKMKWDIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYL 61 K ++W I P RTAVIVVDMQ VFC+P GALYV T I+ I++L AARAA + V+YL Sbjct: 38 KPLVRWPINPLRTAVIVVDMQKVFCEPTGALYVKSTADIVQPIQKLLQAARAAQVMVIYL 97 Query: 62 RHIVRGDGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDL 121 RHIVRGDGSDTGRM D+YPNV++IL R P VE+I+ LAP+ D+IVDKLFYSGFH+TDL Sbjct: 98 RHIVRGDGSDTGRMRDLYPNVDQILARHDPDVEVIEALAPQSDDVIVDKLFYSGFHNTDL 157 Query: 122 DTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAE 181 DT+LR D++TII+CGTVTNVCCETT+RDG HREYKVI LSDANAAMDYPD+G+G +SA Sbjct: 158 DTVLRARDVDTIIVCGTVTNVCCETTIRDGVHREYKVIALSDANAAMDYPDVGFGAVSAA 217 Query: 182 EIQRVTLTVVAYEFGQVSLTEDIIQEIQS 210 ++QR++LT +AYEFG+V+ T ++I+ I+S Sbjct: 218 DVQRISLTTIAYEFGEVTTTAEVIRRIES 246 >C1V9L4.1 C1V9L4_9EURY Nicotinamidase-like amidase Length = 191 Score = 119 bits (299), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 67/197 (34%), Positives = 101/197 (51%), Gaps = 11/197 (5%) Query: 8 DIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYLRHIVRG 67 + PERTAV+VVDMQN FC P G+LY P++E ++ ++ L R AG VVY R + Sbjct: 3 EFDPERTAVVVVDMQNGFCHPGGSLYAPKSEAVVDDVAALVADVRDAGAAVVYTRDVHPP 62 Query: 68 DGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDLDTILRV 127 + D D + E + GT E+++EL D +V K Y FH T+L+ L Sbjct: 63 EQFDDAHYYDEFDRWGEHVVEGTWETELVEELDVREDDHVVVKHTYDAFHQTELEGWLDA 122 Query: 128 HDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAEEIQRVT 187 H I+ ++ CGT+ NVC T R+Y+ + ++DA G I A+ + Sbjct: 123 HGIDDLLFCGTLANVCVLHTAGSAGLRDYRPVLVADAI----------GAIEADH-KEYA 171 Query: 188 LTVVAYEFGQVSLTEDI 204 L + FG+V+ E+I Sbjct: 172 LEHADWLFGEVTTREEI 188 >Q3IT58.1 Q3IT58_NATPD Probable isochorismatase 2; probable nicotinamidase 2; probable N-carbamoylsarcosine amidase 2 EC=3.3.2.1 EC=3.5.1.19 EC=3.5.1.59 Length = 191 Score = 119 bits (297), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 61/162 (37%), Positives = 89/162 (54%) Query: 8 DIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYLRHIVRG 67 ++ PE TAV+VVDMQN FC P+G+LY P +E I +L AR AG VV+ R + Sbjct: 2 ELNPETTAVVVVDMQNGFCHPDGSLYAPGSEAAITPCADLVADAREAGASVVFTRDVHPP 61 Query: 68 DGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDLDTILRV 127 + + D + E + G+ E++ EL+P +++V K Y FH T LD L Sbjct: 62 EQFEDTHYYDEFDRWGEHVLEGSWDAELVAELSPRDDELVVVKHTYDAFHQTQLDGWLES 121 Query: 128 HDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMD 169 H IN ++ICGT+ NVC T R+Y+ I + DA A++ Sbjct: 122 HGINDLLICGTLANVCVLHTAGSAGLRDYRPILVEDAVGAIE 163 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,273,146,980 Number of extensions: 54209837 Number of successful extensions: 134763 Number of sequences better than 1.0e-05: 1817 Number of HSP's gapped: 133229 Number of HSP's successfully gapped: 1819 Length of query: 214 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 84 Effective length of database: 1,835,964,253 Effective search space: 154220997252 Effective search space used: 154220997252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)