BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0021.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGF4.1 B1DGF4_9BACL Transcriptional regulator, AraC family 300 8e-80 Q5WK82.1 Q5WK82_BACSK AraC/XylS family transcriptional regulator 184 1e-44 Q5WK33.1 Q5WK33_BACSK AraC/XylS family transcriptional regulator 182 2e-44 C0ZDD0.1 C0ZDD0_BREBN Probable transcriptional regulator 180 1e-43 B4AJL6.1 B4AJL6_BACPU AraC/XylS family transcriptional regulator 177 9e-43 >B1DGF4.1 B1DGF4_9BACL Transcriptional regulator, AraC family Length = 279 Score = 300 bits (769), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 137/278 (49%), Positives = 190/278 (68%), Gaps = 1/278 (0%) Query: 16 KELQLCVLFAAGYDSITDPSYRWDGLERNDGPLLLFQYTLSGEGVFESENRTYLVTAGQA 75 + L LC LFA G D I D YRWDGL RNDGPLLLFQYT GEG++E T + AGQA Sbjct: 3 EHLALCNLFAIGQDVIRDQHYRWDGLTRNDGPLLLFQYTFDGEGIYERGTETLRIQAGQA 62 Query: 76 FLAEIPGRHRYFYPPDSKEPWQFLFLLFRPDLILPHWRKFLREAGEVPHLSADCAPVRLL 135 F+ EIPG HRY++PPD W F+F+L RP LILP+W + ++ G+ P L A P+RLL Sbjct: 63 FMTEIPGDHRYYFPPDGSH-WSFIFVLIRPSLILPNWEEVKKQLGDTPFLPAGSRPIRLL 121 Query: 136 RMIVNDAAAGRISDPLIASSSIYQFMTELSRLQVTTLRNKDNWSESIRRAADYIEHNFAQ 195 + + +A AGR+ +P ASS +YQF++EL R + + W ++ A YI+ ++ Sbjct: 122 QSMYEEAHAGRVKEPYTASSYVYQFISELCRFALAPQEDAKEWPLKVKEAVSYIDAHYEN 181 Query: 196 MISIEQLSEHVSLSKYHLIRRFSASTGLTPGAYLTRIRIEKAMELLRGSSLSIEAISKQI 255 MIS++QL++ +SLSKYHL+R F+ + G++P YL ++RI++A+ LL+ S S+E I++Q+ Sbjct: 182 MISLDQLADQLSLSKYHLLRLFTRTVGMSPNDYLNQVRIKEAIRLLKESDWSVERIAEQV 241 Query: 256 GYSSGSYFIKAFRSLTGLTPGEFRSGGDSLAYRRLFFD 293 GYS GSYFIK FR +TG TPG FR G L Y R++FD Sbjct: 242 GYSGGSYFIKWFRKITGETPGSFRHGEGQLVYNRIYFD 279 >Q5WK82.1 Q5WK82_BACSK AraC/XylS family transcriptional regulator Length = 297 Score = 184 bits (466), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 97/282 (34%), Positives = 155/282 (54%), Gaps = 3/282 (1%) Query: 3 FTRGTYGFRFAEDKELQLCVLFAAGYDSITDPSYRWDGLER-NDGPLLLFQYTLSGEGVF 61 FTRG++ F F E L L+ G++ TD SY WDG +R D ++FQYT++G GV Sbjct: 7 FTRGSHSFTFHLFPETGLLNLWNVGWEVQTDTSYYWDGNKRAKDNDSIIFQYTIAGSGVL 66 Query: 62 ESENRTYLVTAGQAFLAEIPGRHRYFYPPDSKEPWQFLFLLFRPDLILPHWRKFLREAGE 121 E Y V QAF+ +P HRY+YP ++KEPW+F+++ + + W++ ++++G Sbjct: 67 EIGKERYTVGQHQAFIVSVPSDHRYYYPENAKEPWEFIYISLQGASVQDIWQRLIQKSGP 126 Query: 122 VPHLSADCAPVRLLRMIVNDAAAGRISDPLIASSSIYQFMTELSRLQVTTLRNKDNWSES 181 + L + +R L I D A + DP +A++ YQF+ E R N Sbjct: 127 IVSLPPNSPLIRSLYSIYADTKADGLRDPYLAATQGYQFLIECFRAMAE--ERPQNEPYH 184 Query: 182 IRRAADYIEHNFAQMISIEQLSEHVSLSKYHLIRRFSASTGLTPGAYLTRIRIEKAMELL 241 +RA YIE ++ + I ++ ++E V +S+Y L R F TP +Y+T +R+ KA LL Sbjct: 185 YQRALAYIEAHYHEQICLDNIAEEVGVSRYTLTRLFKKQLHTTPISYVTDLRMRKACSLL 244 Query: 242 RGSSLSIEAISKQIGYSSGSYFIKAFRSLTGLTPGEFRSGGD 283 ++ +I+ I+ +GY +YF K FR TG + +FR GD Sbjct: 245 LHTNRTIKQIAADVGYPDDNYFHKVFRRYTGSSATQFRRNGD 286 >Q5WK33.1 Q5WK33_BACSK AraC/XylS family transcriptional regulator Length = 294 Score = 182 bits (463), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 97/278 (34%), Positives = 150/278 (53%), Gaps = 2/278 (0%) Query: 7 TYGFRFAEDKELQLCVLFAAGYDSITDPSYRWDGLERNDGPLLLFQYTLSGEGVFESENR 66 +YGFRF E + L+ G+++ TD +YRWDG +R + ++FQYTLSG G E Sbjct: 9 SYGFRFQEQPYPFVMNLWNVGWETQTDQAYRWDGTKRMEREKIVFQYTLSGYGELVHEGT 68 Query: 67 TYLVTAGQAFLAEIPGRHRYFYPPDSKEPWQFLFLLFRPDLILPHWRKFLREAGEVPHLS 126 Y V AGQAF +P H Y+YP + EPW+FL++ L + + G V L Sbjct: 69 LYKVEAGQAFFVSLPSAHCYYYPEEETEPWEFLYVTIEGKEALRLQKHMENKYGPVFSLQ 128 Query: 127 ADCAPVRLLRMIVNDAAAGRISDPLIASSSIYQFMTELSRLQVTTLRNKDNWSESIRRAA 186 D P+R L + + AAG I D S Y+F+ LS + + K E + RA Sbjct: 129 KDSEPIRRLFKLYAETAAGEIQDSYTGSQKAYEFI--LSCFRFLEVPPKQVVKEPLARAI 186 Query: 187 DYIEHNFAQMISIEQLSEHVSLSKYHLIRRFSASTGLTPGAYLTRIRIEKAMELLRGSSL 246 YIE N+A+ +++E++++ SKY+ I++F +TP Y+T IRI+KA LL + Sbjct: 187 RYIEANYARPLTLEEIAQEAGWSKYYFIKQFKTELNITPMNYVTIIRIKKAASLLAQTDD 246 Query: 247 SIEAISKQIGYSSGSYFIKAFRSLTGLTPGEFRSGGDS 284 +I ++ Q+G +YF K F+ G++ +FR D+ Sbjct: 247 TIAEVAYQVGMDDPNYFTKVFKKTVGVSASKFRKNEDN 284 >C0ZDD0.1 C0ZDD0_BREBN Probable transcriptional regulator Length = 298 Score = 180 bits (457), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 95/284 (33%), Positives = 158/284 (55%), Gaps = 9/284 (3%) Query: 6 GTYGFRFAEDKELQLCVLFAAGYDSITDPSYRWDGLERNDGPLLLFQYTLSGEGVFESEN 65 G+YGFRF +D + +++ G+ T +Y WDGL R+D +FQYT+SG G + Sbjct: 12 GSYGFRFQDDSRNETANIWSIGWQRQTSANYDWDGLNRSDVGTYVFQYTISGSGRLDIGE 71 Query: 66 RTYLVTAGQAFLAEIPGRHRYFYPPDSKEPWQFLFLLFRPDLILPHWRKFLREAGEVPHL 125 ++Y + AG+ FL E+P HRY++ +S + W+F+++ W G V + Sbjct: 72 KSYPLRAGKTFLVEVPSCHRYYFSGES-DHWEFVYITLVGSQATECWEYIQANGGPVLSI 130 Query: 126 SADCAPVRLLRMIVNDAAAGRISDPLIASSSIYQFMTELSRLQVTTLRNKDNWSES---- 181 + V+LL I + R++D +S+ Y+F+ E R ++N DN S+ Sbjct: 131 PPEAKLVQLLLKIYQEVCEKRVTDLYCSSAKAYEFVMECYRF----MKNIDNPSKQVPDR 186 Query: 182 IRRAADYIEHNFAQMISIEQLSEHVSLSKYHLIRRFSASTGLTPGAYLTRIRIEKAMELL 241 I A +I+ ++ + IS+ ++ LS+Y+LI F TP Y+T++RIEKAM+LL Sbjct: 187 ISEAVCFIQAHYKETISLGDIANAAGLSRYYLINLFREYLNTTPLQYITKLRIEKAMDLL 246 Query: 242 RGSSLSIEAISKQIGYSSGSYFIKAFRSLTGLTPGEFRSGGDSL 285 ++LS+ I+ IGY++ +YF K F+ L G +PG+FR +SL Sbjct: 247 TQTNLSVNDIALAIGYANANYFNKVFKKLIGTSPGQFRECKNSL 290 >B4AJL6.1 B4AJL6_BACPU AraC/XylS family transcriptional regulator Length = 281 Score = 177 bits (449), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 95/280 (33%), Positives = 150/280 (53%), Gaps = 1/280 (0%) Query: 5 RGTYGFRFAEDKELQLCVLFAAGYDSITDPSYRWDGLERNDGPLLLFQYTLSGEGVFESE 64 + + FRF +++ G++ + Y W+G+ER D +FQ TLSG G+ E Sbjct: 3 KEAFAFRFHHSDVTLPAQIWSVGWEVQSSSLYSWNGVERKDQGKCIFQLTLSGHGMIEIG 62 Query: 65 NRTYLVTAGQAFLAEIPGRHRYFYPPDSKEPWQFLFLLFRPDLILPHWRKFLREAGEVPH 124 + + V GQAFL + P ++Y++P DS E W+FL+L + + +F+ + +V Sbjct: 63 EKRFKVLPGQAFLVKSPSAYQYYFPEDS-EHWEFLYLTLYGEACDLCFDQFIDQGKQVMR 121 Query: 125 LSADCAPVRLLRMIVNDAAAGRISDPLIASSSIYQFMTELSRLQVTTLRNKDNWSESIRR 184 + P+RLL+ I ++A+ RI++P SS YQF+ EL + W E I + Sbjct: 122 FHPNSRPIRLLKTIYDEASERRITNPFEGSSLAYQFVMELYSYLPKLEGQMEKWPEPIVQ 181 Query: 185 AADYIEHNFAQMISIEQLSEHVSLSKYHLIRRFSASTGLTPGAYLTRIRIEKAMELLRGS 244 AA + H+F + I + ++ LSK H R F +TG TP YLT IR+EKA LL+ + Sbjct: 182 AALFASHHFHEEIGPDDMAAAARLSKSHFTREFKKATGFTPIHYLTNIRLEKAETLLKTT 241 Query: 245 SLSIEAISKQIGYSSGSYFIKAFRSLTGLTPGEFRSGGDS 284 SIE I+ Q GY + +Y K FR G++P + R D+ Sbjct: 242 KYSIEEIAIQCGYRNANYLNKVFRKKIGMSPKQLRETSDA 281 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.138 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,700,927,659 Number of extensions: 66772978 Number of successful extensions: 170385 Number of sequences better than 1.0e-05: 6822 Number of HSP's gapped: 170667 Number of HSP's successfully gapped: 6822 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)