BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0003.p (186 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1C455.1 B1C455_9FIRM Putative uncharacterized protein 67 5e-10 B5CP50.1 B5CP50_9FIRM Putative uncharacterized protein 65 2e-09 A6VKF9.1 A6VKF9_ACTSZ Transcriptional regulator, TetR family 54 8e-06 >B1C455.1 B1C455_9FIRM Putative uncharacterized protein Length = 168 Score = 67.4 bits (163), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 40/85 (47%), Positives = 53/85 (62%), Gaps = 6/85 (7%) Query: 83 SQISISQITAKAQVSRNSFYRNYVDKEDILLKHIKNLISKWDADYQLINNDSNA--ELYG 140 ++ISI +IT +AQV R SFYRNY KEDIL +++ +I +W N DS + +L Sbjct: 28 NEISIREITTRAQVGRVSFYRNYKRKEDILEQYLFFIIKEWGES----NTDSLSMDKLLK 83 Query: 141 SLFKHLKEYSDFYLLLKKRNLFYLF 165 LF+HL Y DFY LL + L YLF Sbjct: 84 KLFEHLVSYRDFYTLLYTKGLLYLF 108 >B5CP50.1 B5CP50_9FIRM Putative uncharacterized protein Length = 188 Score = 65.5 bits (158), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 36/85 (42%), Positives = 52/85 (61%), Gaps = 2/85 (2%) Query: 82 LSQISISQITAKAQVSRNSFYRNYVDKEDILLKHIKNLISKWDADYQLINNDSNAELYGS 141 L++ISIS+I A V R SFYRNY KED++ K + LI +W+ DY+ N+ + S Sbjct: 45 LNEISISEICETAGVGRMSFYRNYESKEDVIKKQLLQLIQEWEKDYEGKNDPTY--FSES 102 Query: 142 LFKHLKEYSDFYLLLKKRNLFYLFL 166 L +H ++ DFYLLL + L + L Sbjct: 103 LLRHYYKHKDFYLLLYNQGLSNMIL 127 >A6VKF9.1 A6VKF9_ACTSZ Transcriptional regulator, TetR family Length = 168 Score = 53.5 bits (127), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 35/88 (39%), Positives = 47/88 (53%), Gaps = 3/88 (3%) Query: 79 KYELSQISISQITAKAQVSRNSFYRNYVDKEDILLKHIKNLISKWDADYQLINNDSNAEL 138 K ISI++ITA A VSR SFYRN+ KED+L+KH L + + ++ D+ E Sbjct: 23 KKSFDNISITEITALAGVSRISFYRNFESKEDVLIKH---LYERAMTSFTALHADTVRER 79 Query: 139 YGSLFKHLKEYSDFYLLLKKRNLFYLFL 166 LF E D LL + L +LFL Sbjct: 80 LLGLFSVTDELGDVLDLLYSQGLSHLFL 107 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 719,186,490 Number of extensions: 37126916 Number of successful extensions: 64512 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 64604 Number of HSP's successfully gapped: 4 Length of query: 186 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 58 Effective length of database: 1,854,806,283 Effective search space: 107578764414 Effective search space used: 107578764414 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)