BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0036.p (421 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DE02.1 B1DE02_9BACL MATE efflux family protein 341 7e-92 B1DE52.1 B1DE52_9BACL MATE efflux family protein 329 3e-88 A6PNY0.1 A6PNY0_9BACT MATE efflux family protein 194 2e-47 A7VYE7.1 A7VYE7_9CLOT Putative uncharacterized protein 192 5e-47 C4G507.1 C4G507_ABIDE Putative uncharacterized protein 186 4e-45 >B1DE02.1 B1DE02_9BACL MATE efflux family protein Length = 458 Score = 341 bits (875), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 179/372 (48%), Positives = 235/372 (63%), Gaps = 8/372 (2%) Query: 22 NRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSLLFLPCFI 81 +++FNR+L+ LVIPIA+QNLI+A AV+ DVVMLG + Q + F+ Sbjct: 12 DKSFNRQLISLVIPIAMQNLISALAVTVDVVMLGFINQSAMSAVSLAGQ---ITFVLTLF 68 Query: 82 WGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNELMYFFTQD 141 + GIL AQYWGK+D+ TI+RV I C P LM FFT D Sbjct: 69 YMGLATGAGILTAQYWGKQDLATIERVFWIGCLFCTIISFIFFGISLFMPEWLMSFFTND 128 Query: 142 EELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFLNILLNALC 201 +LI YG+ FL ISFSY+ G++QM+ VI E A+ SA SS L +NIL NAL Sbjct: 129 GDLIDYGSSFLQVISFSYVVSGISQMYFSVIRSM--ENARFSAWTSSMCLIINILFNALS 186 Query: 202 VFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERDDAQRKLLKDYL 261 +FVLFP+ P+KAI +VAL+TVVARF ELG C ++S+ +G ++F+ P D Q+ L KD+ Sbjct: 187 IFVLFPDHPQKAIAAVALSTVVARFIELGCCIVHSV-KGKIKFKFPVYDAMQQNLQKDFF 245 Query: 262 RYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCGGIATGGSV 321 RYT PV GNYIVWGGA AAI GH+NADM CGGIA+GGSV Sbjct: 246 RYTLPVLGNYIVWGGALTVT--AAIFGHVNADMVAANSVASVVKNLAIVLCGGIASGGSV 303 Query: 322 LVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLMKPLVFYAVDLNQNAQEYLHGM 381 L+GKYLGN +++ A++AG+++ YA++FG+L+G +L +KPLV V LN AQ YL+GM Sbjct: 304 LIGKYLGNGDMDTARRAGSKINAYAVLFGILAGLSVLFIKPLVLQVVSLNDQAQSYLNGM 363 Query: 382 LYICIYYCIAKS 393 L+IC YYCI KS Sbjct: 364 LFICAYYCIPKS 375 >B1DE52.1 B1DE52_9BACL MATE efflux family protein Length = 462 Score = 329 bits (844), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 184/378 (48%), Positives = 245/378 (64%), Gaps = 8/378 (2%) Query: 16 ITDKNFNRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSLL 75 ++ ++ FN++L+ LVIPIALQNL++A AV+ DVVMLG + Q + Sbjct: 6 LSSSQIDKNFNQQLIRLVIPIALQNLVSALAVTVDVVMLGFINQSAMSAVSLAGQ---VT 62 Query: 76 FLPCFIWGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNELM 135 F+ + GIL AQYWGK+DV TI+RVL+I C P+ LM Sbjct: 63 FVLTLFYMGLASGVGILTAQYWGKQDVKTIERVLSIGCMYSYIVSFCFFGISLFIPDVLM 122 Query: 136 YFFTQDEELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFLNI 195 FT D ELIRYG++FL +SFSYL G +QM+ VI E A+LSA ISS+ L LNI Sbjct: 123 SLFTDDGELIRYGSQFLQVLSFSYLVGGASQMYFSVIRSM--ENARLSAWISSACLILNI 180 Query: 196 LLNALCVFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERDDAQRK 255 LLNAL +FVL+P+ P+KAI +VAL+TV+AR E C ++S+ + ++F+LP+RD QR Sbjct: 181 LLNALSIFVLYPDDPDKAIVAVALSTVLARIIEFICCIVHSV-KWKIKFKLPKRDTIQRN 239 Query: 256 LLKDYLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCGGI 315 L +DYL+YT PVQGNYIVWG A AA+IGHINADM CGGI Sbjct: 240 LQRDYLKYTFPVQGNYIVWGCAIT--ATAAVIGHINADMVAANSVAAVVKNLMIVVCGGI 297 Query: 316 ATGGSVLVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLMKPLVFYAVDLNQNAQ 375 A+GGSVLVGKYLG+ +IEMAK+ G + +Y ++FG+ +G + L++KPL+F+AV L++ AQ Sbjct: 298 ASGGSVLVGKYLGSGQIEMAKRTGNIITFYTIVFGIWAGVMTLIVKPLIFHAVSLDEQAQ 357 Query: 376 EYLHGMLYICIYYCIAKS 393 YL GML+IC YYCI KS Sbjct: 358 YYLSGMLFICAYYCIPKS 375 >A6PNY0.1 A6PNY0_9BACT MATE efflux family protein Length = 452 Score = 194 bits (492), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 121/379 (31%), Positives = 182/379 (48%), Gaps = 8/379 (2%) Query: 14 VNITDKNFNRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKS 73 +N+ + ++ FNR+L+ L PI LQNL+ A +AD +MLG V Q + Sbjct: 1 MNVKELFSDQVFNRKLLRLAFPITLQNLMLALVAAADAIMLGRVSQNAMAAVSLATQIQ- 59 Query: 74 LLFLPCFIWGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNE 133 F+ + G GIL AQYWGK+D + + +++ P + Sbjct: 60 --FVQNMLLGAIVSGVGILGAQYWGKRDSGKMGEIFSLSIHEAVLVSAAFFLGCRFIPEK 117 Query: 134 LMYFFTQDEELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFL 193 LM F D EL+ GA++L S+SYL G++Q +L ++ E A SA ISS + L Sbjct: 118 LMLLFAHDPELVEIGAEYLRVASWSYLLTGISQCYLAIMRVS--EHASRSAWISSGAVIL 175 Query: 194 NILLNALCVFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERDDAQ 253 NI+LNA+ +F F +P + ALATV+AR EL WC + S + + ++ E Sbjct: 176 NIVLNAVFIFGWF-GVPAMGVRGAALATVIARVIELAWCIVSSYEKSFIPLRIRELLRFN 234 Query: 254 RKLLKDYLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCG 313 L +D+ YT PV G++++WG AI+GH+ D C Sbjct: 235 AVLFRDFWHYTLPVLGSFLLWG--IGFTSYTAIMGHLGPDAAAANAVAAVVRDLMCCLCN 292 Query: 314 GIATGGSVLVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLMKPLVFYAVDLNQN 373 GIA G +L+G LG + + K G + + A + G LS +IL PLV ++L + Sbjct: 293 GIAAGAGILIGNELGAGNLSLGKAYGQKAMILAFLIGGLSTLVILSSIPLVSGCLELTEQ 352 Query: 374 AQEYLHGMLYICIYYCIAK 392 A YL GM I Y I + Sbjct: 353 AHHYLVGMFVILSVYMIGR 371 >A7VYE7.1 A7VYE7_9CLOT Putative uncharacterized protein Length = 477 Score = 192 bits (488), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 120/379 (31%), Positives = 190/379 (50%), Gaps = 12/379 (3%) Query: 15 NITDKNFNRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSL 74 + KNF +T LV+PIALQNLI SADVVMLG VG Q + + Sbjct: 32 KLETKNFYKTVG----ALVLPIALQNLINVAVTSADVVMLGRVGETVLSGSSLAGQVQFI 87 Query: 75 LFLPCFIWGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNEL 134 L L FI+G +L AQYWGKKD+ TI++V+ IA P ++ Sbjct: 88 LTL--FIFGLTSGA-AVLTAQYWGKKDMRTIEKVMGIAFRFSIIVSVIFLLAVQIFPAQV 144 Query: 135 MYFFTQDEELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFLN 194 M FT ++ +I G K+L +SFSYL +T ++L V+ E+ ++ + S L +N Sbjct: 145 MSIFTPEQPVIDEGVKYLRIVSFSYLTCAVTHVYLNVMRSV--ERVVIATVTYSVSLIVN 202 Query: 195 ILLNALCVFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERDDAQR 254 I++N + +F L P+ I A+ T+VAR EL +Y+ T+ + F+L + + Sbjct: 203 IIINGILIFGLL-GFPQMGIVGAAIGTLVARIVELLIVVVYAKTKSILNFKLRDLFVRDK 261 Query: 255 KLLKDYLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCGG 314 L +D++RY PV N ++WG A +IGH+ G Sbjct: 262 LLFQDFIRYAIPVTLNELMWGTGCSVI--AVVIGHMGQAAVAANSVAQVTRQLATVVSFG 319 Query: 315 IATGGSVLVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLMKPLVFYAVDLNQNA 374 IA +VL+GK +G + E A+K G R + +++ G+ ++L+ +P+V + L A Sbjct: 320 IAAAAAVLIGKAIGEDNYEEAQKYGTRFVKLSIVAGLAGALVVLIARPIVLQVMTLTPTA 379 Query: 375 QEYLHGMLYICIYYCIAKS 393 Q YL M++I Y+ +A++ Sbjct: 380 QGYLSTMMFIMAYFVVAQA 398 >C4G507.1 C4G507_ABIDE Putative uncharacterized protein Length = 456 Score = 186 bits (472), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 127/393 (32%), Positives = 192/393 (48%), Gaps = 19/393 (4%) Query: 1 MKEGYRVQRQVKQVNITDKNFNRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXX 60 MK G+ + ++ K+ F + L LVIPI +QN I++ SADV+MLG VG Sbjct: 1 MKIGFNISKEQKK-----------FYKALFALVIPITIQNFISSAVNSADVLMLGYVGQS 49 Query: 61 XXQCRRYHWQDKSLLFLPCFIWGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXX 120 Q + LL+ F +G ILA+QYWGK+D +IQ V+ IA Sbjct: 50 ELAAVSLANQFQFLLW--GFFFGVTSGVT-ILASQYWGKRDTDSIQAVMGIAIKISVVVT 106 Query: 121 XXXXXXXXXXPNELMYFFTQDEELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQA 180 P +LM +T D+ L + GA +L I SYL + +Q++L I E+ Sbjct: 107 ALLSICAILFPKQLMTIYTNDDLLRQIGASYLRIIGISYLLLSFSQVYLCTIRSM--ERV 164 Query: 181 QLSALISSSGLFLNILLNALCVFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRG 240 +LS ISS L LN+ LNA+ +F L MP+ + V +AT+++R E+ C + Sbjct: 165 KLSTAISSVALGLNVALNAVFIFGLL-GMPKLGVVGVGIATLISRVVEVSLCVADAAKAK 223 Query: 241 TVRFQLPERDDAQRKLLKDYLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXX 300 + L + L KD+++Y TP N +W A + I+GH+N D+ Sbjct: 224 IFKIDLKLMFGHHKLLFKDFVKYATPALANDFLWTVAFSTY--SIIMGHMNEDVVAAGSV 281 Query: 301 XXXXXXXXXXXCGGIATGGSVLVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLM 360 C +A G SVL+G +G ++E AK+ R L GVL+G +IL + Sbjct: 282 ASTVRNLCSIACFAMAGGASVLLGIKIGEGKMEEAKEYATRSCHATLGLGVLTGLIILAV 341 Query: 361 KPLVFYAVDLNQNAQEYLHGMLYICIYYCIAKS 393 +P+VF + L++ A YL ML I YY I ++ Sbjct: 342 RPIVFMSFTLSEQAYNYLDIMLIISSYYVIGQA 374 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,941,503,864 Number of extensions: 66607786 Number of successful extensions: 188311 Number of sequences better than 1.0e-05: 184 Number of HSP's gapped: 187878 Number of HSP's successfully gapped: 185 Length of query: 421 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 283 Effective length of database: 1,760,596,133 Effective search space: 498248705639 Effective search space used: 498248705639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)