BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0026.p (393 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9XDT1.1 Q9XDT1_9BACI Pectate lyase H 424 e-117 C1S3H7.1 C1S3H7_9SPHI Conserved repeat protein 365 5e-99 B9MKT5.1 B9MKT5_ANATD Pectate disaccharide-lyase EC=4.2.2.9 346 2e-93 C0T1R8.1 C0T1R8_9PSEU Putative carbohydrate-binding protein 284 1e-74 Q9K457.1 Q9K457_STRCO Putative polysaccharide lyase 284 1e-74 >Q9XDT1.1 Q9XDT1_9BACI Pectate lyase H Length = 677 Score = 424 bits (1090), Expect = e-117, Method: Compositional matrix adjust. Identities = 219/394 (55%), Positives = 270/394 (68%), Gaps = 6/394 (1%) Query: 2 SPVSGVLYXXXXXXXXXXXTVGSPTTLGSALTRIAAGGTIYLRGGTYSLSSPVTIDRDNS 61 P G L T+ PTTL SA+TRI G TIY+RGGTY+ S V I+R N+ Sbjct: 281 EPAEGDLIVAPNGQEGNPGTLNQPTTLTSAITRIQPGRTIYMRGGTYAFSETVLIERGNN 340 Query: 62 GTSSSRKNLVAYGSEKPVLDFSGESFASTERGLQIFGHYWYVKGLEVKGAXXXXXXXXXX 121 G +RK +V Y EKPVLDFS ++F RGLQI GHYW+V+G+EVK A Sbjct: 341 GLEGARKRIVGYNGEKPVLDFSAQAFDPMNRGLQINGHYWHVQGIEVKEAGDNGIFIGGN 400 Query: 122 XXXXXXVETHHNRDSGLQIXXXXXXXXXXXXXXXYNQIIGVYSHDNFDPDNGEDADGFAA 181 VETHHN+D+GLQI YN+II VYSH+N+DPD+GEDADGFAA Sbjct: 401 YNRIENVETHHNKDTGLQISRYSSSATRDEWPS-YNEIINVYSHNNYDPDDGEDADGFAA 459 Query: 182 KLTIGPGNVFDKCISAWNTDDGWDLYTKSETGPIGTVTIKNSVAYKNGQTSDGNTTANSD 241 KLT GPGNVFD CI+A+N DDGWDLYTKS+TG I V I+NS+AY NG T G++T+NSD Sbjct: 460 KLTSGPGNVFDGCIAAYNVDDGWDLYTKSDTGAIYPVIIRNSIAYNNGSTEGGHSTSNSD 519 Query: 242 GNGFKLGGDKIAVNHIVTNSVAFQNKKHGFTYNSNQGSIQLTNNTSWSNGQ---SNFAFD 298 GNGFKLGG I VNHIV N++AF NKKHGFTYNSN GSI +TNNTSW+NG SNFAFD Sbjct: 520 GNGFKLGGSNIPVNHIVENNMAFGNKKHGFTYNSNPGSITMTNNTSWNNGTRSGSNFAFD 579 Query: 299 SGTHVFTNNLSFAGTSSDK-TSGTDVSSTNVWWK-SNASVNGKGLAASSADFVSLTPSVV 356 GTH+F NNLSF +SSDK + TD+ +N+WW + S N K L +++DF+SL P+V Sbjct: 580 RGTHLFANNLSFEASSSDKYATSTDIDGSNLWWHNTKGSQNAKNLKVTASDFISLIPTVS 639 Query: 357 RNTDGSFSLGNFLKLAAGSDLIGSGTPSGTNIGA 390 R+ +G+ +G FL+L S L G+GTP+GT+IGA Sbjct: 640 RDANGAPVIGGFLQLTGSSSLKGAGTPAGTDIGA 673 >C1S3H7.1 C1S3H7_9SPHI Conserved repeat protein Length = 750 Score = 365 bits (936), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 197/362 (54%), Positives = 241/362 (66%), Gaps = 2/362 (0%) Query: 21 TVGSPTTLGSALTRIAAGGTIYLRGGTYSLSSPVTIDRDNSGTSSSRKNLVAYGSEKPVL 80 ++ +PTTL +AL I AGGTIYLRGGTYSLS+ V I N+GT+S+ KN+VAY +E PVL Sbjct: 38 SINAPTTLANALATIPAGGTIYLRGGTYSLSASVIIPATNNGTASANKNVVAYSTEVPVL 97 Query: 81 DFSGESFASTERGLQIFGHYWYVKGLEVKGAXXXXXXXXXXXXXXXXVETHHNRDSGLQI 140 +FS ++ A RGL + G YW+ G+ + GA N DSGLQ+ Sbjct: 98 NFSAQAIADANRGLVLDGDYWHFTGITITGAGDNGMLLAGNNNIIEKCIFSKNHDSGLQL 157 Query: 141 XXXXXXXXXXXXXXXYNQIIGVYSHDNFDPDNGEDADGFAAKLTIGPGNVFDKCISAWNT 200 N I+ + DN DPDN EDADGFAAKLT G GNVF CIS N Sbjct: 158 SRYVTSNTTIGSWPSNNLILNCEAFDNQDPDN-EDADGFAAKLTCGTGNVFRGCISHHNI 216 Query: 201 DDGWDLYTKSETGPIGTVTIKNSVAYKNGQTSDGNTTANSDGNGFKLGGDKIAVNHIVTN 260 DDGWDLY K +TGPIG VTI N VAY NG S G+T+ N D NGFKLGG IAVNHI+ Sbjct: 217 DDGWDLYAKDDTGPIGPVTIDNCVAYSNGTLSTGSTSGNGDKNGFKLGGSGIAVNHIIRR 276 Query: 261 SVAFQNKKHGFTYNSNQGSIQLTNNTSWSNGQSNFAFDSG-THVFTNNLSFAGTSSDKTS 319 SVAF N HGFT N+N G+I++TNNTS++N +SNF F G T F NNLSF SSDK++ Sbjct: 277 SVAFNNGHHGFTDNNNPGNIEVTNNTSYNNAESNFNFREGSTATFRNNLSFNAGSSDKSN 336 Query: 320 GTDVSSTNVWWKSNASVNGKGLAASSADFVSLTPSVVRNTDGSFSLGNFLKLAAGSDLIG 379 GT+V S+NVWWK+N S N GL SSADF++ +PSV +NTDGS +LGNFL LA+GSD+I Sbjct: 337 GTEVGSSNVWWKNNVSTNSGGLVVSSADFIATSPSVGKNTDGSPNLGNFLALASGSDMIN 396 Query: 380 SG 381 +G Sbjct: 397 AG 398 >B9MKT5.1 B9MKT5_ANATD Pectate disaccharide-lyase EC=4.2.2.9 Length = 653 Score = 346 bits (888), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 189/369 (51%), Positives = 239/369 (64%), Gaps = 11/369 (2%) Query: 21 TVGSPTTLGSALTRIAAGGTIYLRGGTYSLSSPVTIDRDNSGTSSSRKNLVAYGSEKPVL 80 T+ PTTL A+T + G IY+RGGTY S+ +TI+R+NSGTS++RK + A+ +E+P+L Sbjct: 286 TIDRPTTLEKAITIVQPGQIIYMRGGTYKYSAQITIERNNSGTSNARKCIYAFPNERPIL 345 Query: 81 DFSGESFASTE---RGLQIFGHYWYVKGLEVKGAXXXXXXXXXXXXXXXXVETHHNRDSG 137 DFS +++ S + RGLQI G+YW++KGLEV GA E HHNRDSG Sbjct: 346 DFSSQTYGSVDSNPRGLQINGNYWHIKGLEVMGAADNGIFVGGSYNIIEQCEIHHNRDSG 405 Query: 138 LQIXXXXXXXXXXXXXXXYNQIIGVYSHDNFDPDNGEDADGFAAKLTIGPGNVFDKCISA 197 LQI YN I+ SHDN DPDNGEDADGFA KLT GPGNVF C++ Sbjct: 406 LQISRYISSATRDEWPS-YNLILNCTSHDNMDPDNGEDADGFACKLTAGPGNVFRGCVAY 464 Query: 198 WNTDDGWDLYTKSETGPIGTVTIKNSVAYKNGQTSDGNTTANSDGNGFKLGGDKIAVNHI 257 +N DDGWDLYTKSETG IG V I++ VAY +GQTS G+ T++SDGNGFKLGG I VNH Sbjct: 465 YNVDDGWDLYTKSETGAIGEVLIEDCVAYGHGQTSTGSATSSSDGNGFKLGGSNIKVNHT 524 Query: 258 VTNSVAFQNKKHGFTYNSNQGSIQLTNNTSWSNGQS----NFAFDSGTHVFTNNLSFAGT 313 V +AF N KHGFTYNSN GSI + N T ++NG NF F+ GTHV N LS+ + Sbjct: 525 VRRCIAFNNNKHGFTYNSNPGSITVENCTGYNNGLKVSGRNFYFEEGTHVLKNCLSYKES 584 Query: 314 -SSDKTSGTDVSSTNVWWKSNASVNGKGLAASSADFVSLTPSVVRNTDGSFSLGNFLKLA 372 SSD SGT ++ V W + ++ G + DF SLTP++ RN+DG +LG+FLK Sbjct: 585 ASSDLVSGTIINC--VLWSNRQAIKLNGQLVTDNDFYSLTPTITRNSDGGLNLGDFLKPK 642 Query: 373 AGSDLIGSG 381 GS L G G Sbjct: 643 PGSGLEGIG 651 >C0T1R8.1 C0T1R8_9PSEU Putative carbohydrate-binding protein Length = 574 Score = 284 bits (726), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 160/326 (49%), Positives = 200/326 (61%), Gaps = 5/326 (1%) Query: 7 VLYXXXXXXXXXXXTVGSPTTLGSALTRIAAGGTIYLRGGTYSLSSPVTIDRDNSGTSSS 66 +Y T +PTTL SA++R+A+GGTIYLRGGTY SS V+I + +GT+S Sbjct: 216 AIYASPSGTDGAAGTEAAPTTLTSAISRVASGGTIYLRGGTYRFSSTVSIPQGRNGTASK 275 Query: 67 RKNLVAYGSEKPVLDFSGESFASTERGLQIFGHYWYVKGLEVKGAXXXXXXXXXXXXXXX 126 R L AY E PVL+FS +S RGL + YW+V+G+ V+ A Sbjct: 276 RTQLFAYSGETPVLNFSAQSEDPANRGLALNASYWHVRGIVVERAGDNGIFISGSNNVVE 335 Query: 127 XVETHHNRDSGLQIXXXXXXXXXXXXXXXYNQIIGVYSHDNFDPDNGEDADGFAAKLTIG 186 T NRDSGLQ+ N I+ SHDN D D GEDADGFAAKLT G Sbjct: 336 RTVTRFNRDSGLQLSRELSSTPKDQWPAN-NLILSSESHDNADSD-GEDADGFAAKLTSG 393 Query: 187 PGNVFDKCISAWNTDDGWDLYTKSETGPIGTVTIKNSVAYKNGQTSDGNTTANSDGNGFK 246 PGNVF +S N DDGWDLYTK++TGPIG VTI++S+AY NG SDG+ N D NGFK Sbjct: 394 PGNVFRYTVSHNNIDDGWDLYTKTDTGPIGAVTIEDSLAYGNGTLSDGSQAGNGDRNGFK 453 Query: 247 LGGDKIAVNHIVTNSVAFQNKKHGFTYNSNQGSIQLTNNTSWSNGQSNFAFDSGTHVFTN 306 LGG+ IAVNH+V ++A+ N KHGFTYN N G I ++NN S + + NF FD GT VF N Sbjct: 454 LGGEDIAVNHVVQRTIAYDNGKHGFTYNRNLGKITMSNNVSVDSTERNFNFDGGTSVFRN 513 Query: 307 NLSF--AGTSSDKTSGTDVSSTNVWW 330 N+S +G + DKT G DV S+N +W Sbjct: 514 NVSCYSSGGTKDKTIG-DVDSSNQFW 538 >Q9K457.1 Q9K457_STRCO Putative polysaccharide lyase Length = 554 Score = 284 bits (726), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 160/335 (47%), Positives = 200/335 (59%), Gaps = 4/335 (1%) Query: 3 PVSGVLYXXXXXXXXXXXTVGSPTTLGSALTRIAAGGTIYLRGGTYSLSSPVTIDRDNSG 62 P LY T PTTL SA+ R++ GGTIYLRGGTY + VT+ + N G Sbjct: 192 PSGSALYVSPGGSDGASGTESDPTTLTSAIDRVSPGGTIYLRGGTYRYAQTVTVPQGNDG 251 Query: 63 TSSSRKNLVAYGSEKPVLDFSGESFASTERGLQIFGHYWYVKGLEVKGAXXXXXXXXXXX 122 TS R L AY E PVL+FS +S +S+ RGL + G YW+VKG+ V+ A Sbjct: 252 TSGDRTELTAYPGETPVLNFSAQSESSSNRGLAVNGSYWHVKGIVVEHAGDNGIFVGGSN 311 Query: 123 XXXXXVETHHNRDSGLQIXXXXXXXXXXXXXXXYNQIIGVYSHDNFDPDNGEDADGFAAK 182 T NRD+GLQ+ N I+ SHDN D D GEDADGFAAK Sbjct: 312 NVFERTVTRFNRDTGLQLSRTTSDTPRDQWPSN-NLILSAESHDNADSD-GEDADGFAAK 369 Query: 183 LTIGPGNVFDKCISAWNTDDGWDLYTKSETGPIGTVTIKNSVAYKNGQTSDGNTTANSDG 242 LT+GPGNVF +S N DDGWDLYTKSETG IG VTI++S+AY+NG SDG ++ D Sbjct: 370 LTVGPGNVFRDAVSHNNIDDGWDLYTKSETGAIGAVTIEDSLAYENGTLSDGTQNSSGDR 429 Query: 243 NGFKLGGDKIAVNHIVTNSVAFQNKKHGFTYNSNQGSIQLTNNTSWSNGQSNFAFDSGTH 302 NG+KLGG+ I V+H+V S+A++N KHGFTYNSN G++ +++N S N + NF+FD GT Sbjct: 430 NGYKLGGEDIEVDHVVRRSIAYRNGKHGFTYNSNPGTMTISDNVSIDNAERNFSFDKGTS 489 Query: 303 VFTNNLSFAGT--SSDKTSGTDVSSTNVWWKSNAS 335 VF NN S S+DKT G S W SN S Sbjct: 490 VFRNNTSCRADDGSNDKTVGDADGSNQFWTGSNGS 524 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.310 0.129 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,331,424,935 Number of extensions: 101094541 Number of successful extensions: 221861 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 221815 Number of HSP's successfully gapped: 81 Length of query: 393 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 255 Effective length of database: 1,760,596,133 Effective search space: 448952013915 Effective search space used: 448952013915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)