BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0019.p (308 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8FA17.1 B8FA17_DESAA Alpha/beta hydrolase fold-3 domain protein 221 5e-56 Q6RJM1.1 Q6RJM1_9BACT Lipase/esterase 182 3e-44 Q6RJL9.1 Q6RJL9_9BACT Lipase/esterase 182 3e-44 B9M8B7.1 B9M8B7_GEOSF Alpha/beta hydrolase fold-3 domain protein 181 6e-44 B8Y561.1 B8Y561_9BACT Lipolytic enzyme 181 6e-44 >B8FA17.1 B8FA17_DESAA Alpha/beta hydrolase fold-3 domain protein Length = 331 Score = 221 bits (564), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 120/283 (42%), Positives = 167/283 (59%), Gaps = 8/283 (2%) Query: 28 TAMQIRLELAESAKRLQQLPF-ISAEKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGG 86 T + + + E+ +L LP+ E+ + GL+ EWV A K PE+ +KV+LYFHGGG Sbjct: 54 TPVDVTRDAVENLSKLATLPWGTKVEETSVAGLKAEWVRARK--TPENSKKVLLYFHGGG 111 Query: 87 FISGSCEVYRDLAARISAASGVPVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPED 146 F GS +R LAA +S ASG+PVL EYRLAPE +P AN DC +Y LL+ G +PE+ Sbjct: 112 FFCGSAASHRYLAAMLSKASGMPVLVPEYRLAPEFKFPTANVDCLDSYLWLLDQGYKPEN 171 Query: 147 IILGGDSVGATLALMTMITLRESGGRLPAAAFLLSPHGDLVHLDGESYDSNAELDP--TG 204 I LGGDS G L LMT+++++E +P AA ++SP D V DGES S ++ DP G Sbjct: 172 IALGGDSAGGCLVLMTLLSIKEKNLPMPKAAIMISPMTDAVSFDGESLKSRSQADPLFDG 231 Query: 205 SREGNRRILRDYLGGYAGEPPALLSXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRA 264 G + + G + P L S IQAGD EVLLSD+ +LA+R + Sbjct: 232 GDMGKHMM---WYFGPKDQRPELASPLNMDLSGLPPLFIQAGDDEVLLSDSTRLADRAKK 288 Query: 265 ERGEVTLEIWENMWSVFQMMSAMLPEAQEAIGNIGSFVRKTWK 307 +V LE+WENMW VFQ + PEA+ A+ ++G F+++ K Sbjct: 289 AGVKVKLEVWENMWHVFQSFGVICPEARRAVNHLGLFLKQELK 331 >Q6RJM1.1 Q6RJM1_9BACT Lipase/esterase Length = 296 Score = 182 bits (463), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 103/255 (40%), Positives = 145/255 (56%), Gaps = 6/255 (2%) Query: 49 ISAEKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGV 108 + +E + G++ EWV A D + +LY HGGG++ GS +R LA RIS A+ Sbjct: 44 VKSEPVNAGGVKSEWVTAPGA----DAGRAVLYLHGGGYVIGSINTHRSLAGRISRAAKA 99 Query: 109 PVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRE 168 VL ++YRLAPEH +PAA +D +AY +L TGL+P I + GDS G L + T++ +R+ Sbjct: 100 RVLVIDYRLAPEHPFPAAVEDSVAAYRWMLSTGLKPSRIAVAGDSAGGGLTVATLVAIRD 159 Query: 169 SGGRLPAAAFLLSPHGDLVHLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPPALL 228 + LPAA LSP D+ + G+S S A DP ++G + + YLGG P L Sbjct: 160 AKLPLPAAGVPLSPWVDMEGV-GDSMKSKAAADPMVQKDGLIEMAKAYLGGKDPRTP-LA 217 Query: 229 SXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAML 288 + IQ G E LL D+ +LAER R +VTLE WENM VFQ+ + +L Sbjct: 218 APLYADLAGLPPLLIQVGTAETLLDDSTRLAERARKAGVKVTLEPWENMVHVFQIFAPIL 277 Query: 289 PEAQEAIGNIGSFVR 303 E Q+AI IG+F+R Sbjct: 278 DEGQQAIDKIGAFIR 292 >Q6RJL9.1 Q6RJL9_9BACT Lipase/esterase Length = 296 Score = 182 bits (462), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 102/255 (40%), Positives = 146/255 (57%), Gaps = 6/255 (2%) Query: 49 ISAEKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGV 108 + +E + G++ EWV A D + +LY HGGG++ GS +R +A RIS A+ Sbjct: 44 VKSEPVNAGGVKSEWVTAPGA----DAGRAVLYLHGGGYVIGSINTHRSVAGRISRAAKA 99 Query: 109 PVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRE 168 VL ++YRLAPEH +PAA +D +AY +L TGL+P I + GDS G L + T++ +R+ Sbjct: 100 RVLVIDYRLAPEHPFPAAVEDSVAAYRWMLSTGLKPSRIAVAGDSAGGGLTVATLVAIRD 159 Query: 169 SGGRLPAAAFLLSPHGDLVHLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPPALL 228 + LPAA LSP D+ + G+S S A +DP ++G + + YLGG P L Sbjct: 160 AKLPLPAAGVPLSPWVDMEGV-GDSMKSKAAVDPMVQKDGLIEMAKAYLGGKDPRTP-LA 217 Query: 229 SXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAML 288 + IQ G E LL D+ +LAER R +VTLE WENM VFQ+ + +L Sbjct: 218 APLYADLAGLPPLLIQVGTAETLLDDSTRLAERARKAGVKVTLEPWENMIHVFQIFAPIL 277 Query: 289 PEAQEAIGNIGSFVR 303 E Q+AI IG+F+R Sbjct: 278 DEGQQAIDKIGAFIR 292 >B9M8B7.1 B9M8B7_GEOSF Alpha/beta hydrolase fold-3 domain protein Length = 307 Score = 181 bits (460), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 105/280 (37%), Positives = 152/280 (54%), Gaps = 33/280 (11%) Query: 38 ESAKRLQQLPFISAEKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRD 97 ++A+R+ + + G+ EWV+ + + KV++Y HGG ++ GSC +R Sbjct: 35 DAAERIPLPKDVRTQTVVAGGVDAEWVHP----HEATKEKVLMYLHGGAYVMGSCNTHRP 90 Query: 98 LAARISAASGVPVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGAT 157 LAA+I+ A G+ L +EYRLAPEH YPAA D +AY LL G+ P+D+++ GDS G Sbjct: 91 LAAQIARACGMRALLLEYRLAPEHPYPAAIHDSVAAYRWLLYNGVHPDDLVIAGDSAGGG 150 Query: 158 LALMTMITLRESGGRLPAAAFLLSPHGDLVHLDGESYDSNAELDP-------------TG 204 LAL T+++LR+SG +PAAA ++P DL GES + A+ DP G Sbjct: 151 LALATLLSLRDSGEPMPAAAVCITPWADLTGA-GESVKTRAKADPCLWPDHFSFAGHYAG 209 Query: 205 SREGNRRILRDYLGGYAGEPPALLSXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRA 264 + + +L AG PP L IQA +LLSD+ LA+R + Sbjct: 210 AHDRCDPLLSPLYADLAGLPPLL---------------IQAAGDHILLSDSTGLAQRAKD 254 Query: 265 ERGEVTLEIWENMWSVFQMMSAMLPEAQEAIGNIGSFVRK 304 VTLE+W+ MW F + + LPEA+ AI IGSFVR+ Sbjct: 255 AGVAVTLEVWQGMWHAFHLHAPQLPEARSAIEAIGSFVRR 294 >B8Y561.1 B8Y561_9BACT Lipolytic enzyme Length = 307 Score = 181 bits (460), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 112/297 (37%), Positives = 160/297 (53%), Gaps = 8/297 (2%) Query: 9 EMLTAIKNHLKQNNQTAGKTAMQIRLELAESAKRLQQLPF-ISAEKADMNGLQGEWVNAC 67 + L AI L+ G ++ R L E+ + +P + E D G+ GEW+ A Sbjct: 4 QQLQAIIQALRSTPGQHGAD-LEQRRALMEAVTWMFPVPDDVKREPVDAGGVPGEWI-AA 61 Query: 68 KGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGVPVLTVEYRLAPEHVYPAAN 127 G PE +VI Y HGGG++ GS +R + + +S A+G L ++YRLAPE+ +PAA Sbjct: 62 PGAAPE---RVIYYLHGGGYVIGSINSHRQMVSHLSRAAGARALAIDYRLAPENPFPAAV 118 Query: 128 DDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRESGGRLPAAAFLLSPHGDLV 187 +D +AY LL TG+ P +++ GDS G L + T++ LR++G LPAAA LSP D+ Sbjct: 119 EDATAAYRWLLSTGVDPARVVIAGDSAGGGLTVATLVALRDAGDPLPAAAVCLSPWVDME 178 Query: 188 HLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPPALLSXXXXXXXXXXXXXIQAGD 247 L GES + A+LDP + YLGG P L + I G Sbjct: 179 GL-GESMTTKADLDPMIQPGDILEGAKAYLGGADPRTP-LAAPLYADLTGLPPLLIHVGT 236 Query: 248 QEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAMLPEAQEAIGNIGSFVRK 304 E LL D+ +LAER +A V L++W+ M VFQ +AMLPE Q+AI IG F+R+ Sbjct: 237 SETLLDDSTRLAERAKAAGVNVNLQVWDEMIHVFQFFAAMLPEGQQAIDRIGEFIRE 293 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,768,228,697 Number of extensions: 72057018 Number of successful extensions: 181907 Number of sequences better than 1.0e-05: 2259 Number of HSP's gapped: 181090 Number of HSP's successfully gapped: 2263 Length of query: 308 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 173 Effective length of database: 1,788,859,178 Effective search space: 309472637794 Effective search space used: 309472637794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)