BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0012.p (255 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D603.1 B1D603_9BACL Beta-galactosidase EC=3.2.1.23 267 7e-70 C0W658.1 C0W658_9ACTO Possible beta-galactosidase EC=3.2.1.23 184 9e-45 B1RFC4.1 B1RFC4_CLOPE Beta-galactosidase EC=3.2.1.23 116 2e-24 B1BS30.1 B1BS30_CLOPE Beta-galactosidase EC=3.2.1.23 113 2e-23 B1V194.1 B1V194_CLOPE Beta-galactosidase EC=3.2.1.23 113 2e-23 >B1D603.1 B1D603_9BACL Beta-galactosidase EC=3.2.1.23 Length = 681 Score = 267 bits (682), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 127/253 (50%), Positives = 170/253 (67%) Query: 2 WQSNKEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQ 61 WQS + WFK LQ RH+P D ++LRS TTLE+L Y+V++YPH AIM+DETA LL +YV+Q Sbjct: 427 WQSMRSWFKQLQYRHVPADSIFLRSNTTLEELAKYKVVVYPHSAIMTDETAELLTRYVEQ 486 Query: 62 GGTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEAL 121 GG L G RTGYKD GQCYMRPFPG LCGITVE+FT+++G ++W Sbjct: 487 GGKLFLGARTGYKDMNGQCYMRPFPGPVAGLCGITVEDFTLIKGKVNAAELQWEGRLSEG 546 Query: 122 TGADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKIL 181 T A FN++L V+ EV+ Y+S+YY G+PA+ R G+G+VWY GA ++ IL Sbjct: 547 TKAAGFNEVLSVQNPEAEVVARYSSEYYQGEPALIRRNVGEGQVWYLGAAYSEPVVDGIL 606 Query: 182 DSLNLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPKTDLLTGEILS 241 D + LSSP A+ + P EVE+ IR++ + YAFLLNYS++ VT+ +K+ TDLLTG L Sbjct: 607 DEIGLSSPLADRITAPAEVEIGIRASKDASYAFLLNYSDKPVTVQVKQRATDLLTGAALE 666 Query: 242 GSWKLEGFGVRVL 254 + +GV VL Sbjct: 667 NEVVMPAYGVMVL 679 >C0W658.1 C0W658_9ACTO Possible beta-galactosidase EC=3.2.1.23 Length = 678 Score = 184 bits (466), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 98/250 (39%), Positives = 143/250 (57%), Gaps = 3/250 (1%) Query: 4 SNKEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQGG 63 S+ F A Q H P D +YL ++++LEDL Y++L+ PH A+M + + LL +V+ GG Sbjct: 429 SDDAVFAACQLSHTPLDFVYLDARSSLEDLERYRLLLVPHLAMMDEAMSELLRAFVEAGG 488 Query: 64 TLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEALTG 123 T++FG RTGYKD+ G C MRP PG G+ V +FT + + W E Sbjct: 489 TVVFGARTGYKDSNGLCPMRPMPGLVGQWAGVKVTDFTNLGQREEGLTATWD---EQRLP 545 Query: 124 ADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKILDS 183 F+++L D VEV+ + +DYYAG+PA+TR GKG+V+Y GA F+ E ++L Sbjct: 546 VPVFSEVLEARADDVEVLARFDADYYAGEPALTRRTVGKGQVYYLGACFSEEMVRRLLTE 605 Query: 184 LNLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPKTDLLTGEILSGS 243 L SP A +E P VEL +R + + F+LNY +E + L TDLL+G G Sbjct: 606 TGLVSPLAGLIEAPEGVELAVRRKGERQWLFVLNYQDEPQVLRLPTAYTDLLSGTSEQGE 665 Query: 244 WKLEGFGVRV 253 ++E +GVRV Sbjct: 666 VEVEPYGVRV 675 >B1RFC4.1 B1RFC4_CLOPE Beta-galactosidase EC=3.2.1.23 Length = 689 Score = 116 bits (290), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 82/263 (31%), Positives = 134/263 (50%), Gaps = 22/263 (8%) Query: 6 KEWFKALQKRH-------IPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQY 58 K++F+ QK + IP DV+ + D Y+V++ P ++ L+++ Sbjct: 432 KDYFETCQKHYKAFWDMSIPTDVINMDC-----DFSKYKVVVAPMLYMVRPGVGERLEEF 486 Query: 59 VQQGGTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLP-TSIRWGND 117 V GGTL+ +G D C++ FPG + + GI EE + + S+ GN Sbjct: 487 VNNGGTLVTTYWSGIVDENDLCFLGGFPGPLKKVTGIWAEELDALYDEDVNYVSVEEGNS 546 Query: 118 PEALTG---ADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNA 174 + G A F D++H E EV+ TY +D+Y G PA+T N GKG+ +Y N Sbjct: 547 L-GMKGEYEARIFCDLIHSE--GAEVLATYKTDFYKGMPALTCNNFGKGQAYYIAFRNND 603 Query: 175 EAAHKILDSL--NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPK- 231 E SL L+ A ++LP V Q+R ++K+ + FL+N+S E TI +K+ Sbjct: 604 EFLSDFYSSLAKKLTLKRAIEIDLPKGVNAQVRMDEKNEFVFLMNFSSEEKTIDIKDLDL 663 Query: 232 TDLLTGEILSGSWKLEGFGVRVL 254 TD+LTGE ++ ++E +GVR++ Sbjct: 664 TDMLTGEKVTKEMEIEPYGVRIV 686 >B1BS30.1 B1BS30_CLOPE Beta-galactosidase EC=3.2.1.23 Length = 689 Score = 113 bits (282), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 79/263 (30%), Positives = 134/263 (50%), Gaps = 22/263 (8%) Query: 6 KEWFKALQKRH-------IPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQY 58 K++F+ QK + IP DV+ + D Y+V++ P ++ L+++ Sbjct: 432 KDYFETCQKHYKAFWDMSIPTDVINMDC-----DFSKYKVVVAPMLYMVRPGVGERLEEF 486 Query: 59 VQQGGTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLP-TSIRWGND 117 V+ GGTL+ +G D C++ FPG + + GI EE + + SI GN Sbjct: 487 VKNGGTLVTTYWSGIVDENDLCFLGGFPGPLKKVTGIWAEELDALYDEDVNYVSIEEGNS 546 Query: 118 PEALTG---ADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNA 174 + G A F D++H E EV+ TY +D+Y G PA+T N G+G+ +Y N Sbjct: 547 L-GMKGEYEARIFCDLIHSE--GAEVLATYKTDFYKGMPALTCNNFGEGQAYYIAFRNND 603 Query: 175 EAAHKILDSL--NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPK- 231 E SL L+ A ++LP + Q+R ++K+ + F +N+S E TI +K+ Sbjct: 604 EFLSDFYSSLAKKLTLKRAIEIDLPKGINAQVRMDEKNEFVFFMNFSSEEKTIDIKDLDL 663 Query: 232 TDLLTGEILSGSWKLEGFGVRVL 254 TD++TGE ++ ++E +GVR++ Sbjct: 664 TDMVTGEKVTKEMEIEPYGVRIV 686 >B1V194.1 B1V194_CLOPE Beta-galactosidase EC=3.2.1.23 Length = 689 Score = 113 bits (282), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 79/263 (30%), Positives = 134/263 (50%), Gaps = 22/263 (8%) Query: 6 KEWFKALQKRH-------IPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQY 58 K++F+ QK + IP DV+ + D Y+V++ P ++ L+++ Sbjct: 432 KDYFETCQKHYKAFWDMSIPTDVINMDC-----DFSKYKVVVAPMLYMVRPGVGERLEEF 486 Query: 59 VQQGGTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLP-TSIRWGND 117 V+ GGTL+ +G D C++ FPG + + GI EE + + SI GN Sbjct: 487 VKNGGTLVTTYWSGIVDENDLCFLGGFPGPLKKVTGIWAEELDALYDEDVNYVSIEEGNS 546 Query: 118 PEALTG---ADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNA 174 + G A F D++H E EV+ TY +D+Y G PA+T N G+G+ +Y N Sbjct: 547 L-GMKGEYEARIFCDLIHSE--GAEVLATYKTDFYKGMPALTCNNFGEGQAYYIAFRNND 603 Query: 175 EAAHKILDSL--NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPK- 231 E SL L+ A ++LP + Q+R ++K+ + F +N+S E TI +K+ Sbjct: 604 EFLSDFYSSLAKKLTLKRAIEIDLPKGINAQVRMDEKNEFVFFMNFSSEEKTIDIKDLDL 663 Query: 232 TDLLTGEILSGSWKLEGFGVRVL 254 TD++TGE ++ ++E +GVR++ Sbjct: 664 TDMVTGEKVTKEMEIEPYGVRIV 686 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,666,250,764 Number of extensions: 70407555 Number of successful extensions: 145160 Number of sequences better than 1.0e-05: 256 Number of HSP's gapped: 144793 Number of HSP's successfully gapped: 256 Length of query: 255 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 122 Effective length of database: 1,807,701,208 Effective search space: 220539547376 Effective search space used: 220539547376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)