BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0011.p (222 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D603.1 B1D603_9BACL Beta-galactosidase EC=3.2.1.23 301 3e-80 C0W658.1 C0W658_9ACTO Possible beta-galactosidase EC=3.2.1.23 201 3e-50 O54315.1 O54315_9DEIN Beta-galactosidase EC=3.2.1.23 128 3e-28 O69315.1 BGAL_THETH Beta-galactosidase EC=3.2.1.23 126 2e-27 Q0GA06.1 Q0GA06_THETH Beta-galactosidase 126 2e-27 >B1D603.1 B1D603_9BACL Beta-galactosidase EC=3.2.1.23 Length = 681 Score = 301 bits (770), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 136/205 (66%), Positives = 167/205 (81%), Gaps = 4/205 (1%) Query: 1 LNCEINVFYSESDHLAFREWVQAKYKTLDQLNDAWGTVFWNQTYTSWSQVFLXXXXXXXX 60 NCE NVFY+E+DH AFR W++ KY+TL++LN AWGTVFW+QTYT W Q+ L Sbjct: 152 FNCEKNVFYAEADHAAFRVWLKEKYETLEKLNKAWGTVFWSQTYTDWEQLHLSQTDSPNP 211 Query: 61 XXXXHQALDEKRFISDNTISFAKIQADIIRELAPAQWVTTNGLFGHLDSHELNEKLLDFF 120 H ALDEKRFISDN ISFAK+QADIIRELAP WVTTNGLFGHLD+H++ ++LLDFF Sbjct: 212 ----HLALDEKRFISDNVISFAKLQADIIRELAPQHWVTTNGLFGHLDNHKMTDELLDFF 267 Query: 121 SYDSYPQFASIHTDPEETEPLRDRSWSLSLSAVRSISPNFCIMEQQSGPGGWVNRMNMPS 180 SYDSYPQF+S+ + ET+PL DR WS++L+ VRSISPNFC+MEQQSGPGGWV+R+ M + Sbjct: 268 SYDSYPQFSSVFYNDNETDPLLDRQWSINLTNVRSISPNFCVMEQQSGPGGWVDRIEMGT 327 Query: 181 PKPGQMRLWTYQSIAHGADKLLYFR 205 P+PGQ+RLW+YQS+ HG D L+YFR Sbjct: 328 PRPGQIRLWSYQSVLHGTDLLVYFR 352 >C0W658.1 C0W658_9ACTO Possible beta-galactosidase EC=3.2.1.23 Length = 678 Score = 201 bits (512), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 105/206 (50%), Positives = 137/206 (66%), Gaps = 2/206 (0%) Query: 1 LNCEINVFYSESDHLAFREWVQAKYKTLDQLNDAWGTVFWNQTYTSWSQVFLXXXXXXXX 60 +NCEI+ FYSE DHLA+RE+++A+ TL+++N A G FW++ Y+ WSQV L Sbjct: 149 INCEIDEFYSEGDHLAYREFLRAELGTLEEVNRALGLTFWSRQYSDWSQVRLSGRGLHDA 208 Query: 61 XXXXHQALDEKRFISDNTISFAKIQADIIRELAP-AQWVTTNGLFGHLDSHELNEKLLDF 119 H L EKRF S + + FA+ Q +I+R P + ++TTNGLFG+L+ EL + LDF Sbjct: 209 VNP-HSLLWEKRFFSHSAVRFARKQVEILRSHVPDSHFITTNGLFGNLNYTELMKAGLDF 267 Query: 120 FSYDSYPQFASIHTDPEETEPLRDRSWSLSLSAVRSISPNFCIMEQQSGPGGWVNRMNMP 179 ++DSYP FA + LRDR WS SL+ RSIS F IMEQQSG GW +RM P Sbjct: 268 ITFDSYPNFAFDVDRDLAADRLRDRRWSWSLTRTRSISRCFGIMEQQSGAHGWTSRMMGP 327 Query: 180 SPKPGQMRLWTYQSIAHGADKLLYFR 205 +PKPGQMRLWT QS+AHGAD + YFR Sbjct: 328 APKPGQMRLWTMQSVAHGADFVSYFR 353 >O54315.1 O54315_9DEIN Beta-galactosidase EC=3.2.1.23 Length = 645 Score = 128 bits (322), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 81/206 (39%), Positives = 114/206 (55%), Gaps = 14/206 (6%) Query: 9 YSESDHLAFREWVQAKYKTLDQLNDAWGTVFWNQTYTSWSQVFLXXXXXXXXXXXXHQAL 68 Y + AFR W++AKY T+D LN AWGTVFW+QTY + +V L L Sbjct: 151 YCPNCREAFRGWLRAKYGTIDALNAAWGTVFWSQTYRDFGEVELPHLTVAEANPS--HLL 208 Query: 69 DEKRFISDNTISFAKIQADIIRELAPAQWVTTN--GLFGHLDSHELNEKLLDFFSYDSYP 126 D RF SD ++ + Q D++R+ AP +++T N G F LD L E LDF ++DSYP Sbjct: 209 DYYRFASDQVRAYNRFQVDLLRDNAPGRFITHNFMGFFTDLDPFALAED-LDFAAWDSYP 267 Query: 127 -QFASIHTDPEETEPLRDRSWSLSLSAV-----RSISPN-FCIMEQQSGPGGWVNRMNMP 179 F + P+E + R+ ++A R + F +MEQQ GP W + P Sbjct: 268 LGFTDLMPLPQEEKVQWARTGHPDVAAFHHDLYRGVGRGRFWVMEQQPGPVNWAP--HNP 325 Query: 180 SPKPGQMRLWTYQSIAHGADKLLYFR 205 SP PG +RLWT+++IAHGA+ + YFR Sbjct: 326 SPAPGMVRLWTWEAIAHGAEVVSYFR 351 >O69315.1 BGAL_THETH Beta-galactosidase EC=3.2.1.23 Length = 645 Score = 126 bits (316), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 78/199 (39%), Positives = 113/199 (56%), Gaps = 14/199 (7%) Query: 16 AFREWVQAKYKTLDQLNDAWGTVFWNQTYTSWSQVFLXXXXXXXXXXXXHQALDEKRFIS 75 AFR W++A+Y T++ LN+AWGT FW+Q Y S+++V L LD RF S Sbjct: 158 AFRGWLEARYGTIEALNEAWGTAFWSQRYRSFAEVELPHLTVAEPNPS--HLLDYYRFAS 215 Query: 76 DNTISFAKIQADIIRELAPAQWVTTN--GLFGHLDSHELNEKLLDFFSYDSYP-QFASIH 132 D +F ++Q +I+R AP ++VT N G F LD+ L + LDF S+DSYP F + Sbjct: 216 DQVRAFNRLQVEILRAHAPGKFVTHNFMGFFTDLDAFALAQD-LDFASWDSYPLGFTDLM 274 Query: 133 TDPEETEPLRDRSWSLSLSAV-----RSISPN-FCIMEQQSGPGGWVNRMNMPSPKPGQM 186 P E + R+ ++A R + F +MEQQ GP W + PSP PG + Sbjct: 275 PLPPEEKLRYARTGHPDVAAFHHDLYRGVGRGRFWVMEQQPGPVNWAP--HNPSPAPGMV 332 Query: 187 RLWTYQSIAHGADKLLYFR 205 RLWT++++AHGA+ + YFR Sbjct: 333 RLWTWEALAHGAEVVSYFR 351 >Q0GA06.1 Q0GA06_THETH Beta-galactosidase Length = 645 Score = 126 bits (316), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 78/199 (39%), Positives = 113/199 (56%), Gaps = 14/199 (7%) Query: 16 AFREWVQAKYKTLDQLNDAWGTVFWNQTYTSWSQVFLXXXXXXXXXXXXHQALDEKRFIS 75 AFR W++A+Y T++ LN+AWGT FW+Q Y S+++V L LD RF S Sbjct: 158 AFRGWLEARYGTIEALNEAWGTAFWSQRYRSFAEVELPHLTVAEPNPS--HLLDYYRFAS 215 Query: 76 DNTISFAKIQADIIRELAPAQWVTTN--GLFGHLDSHELNEKLLDFFSYDSYP-QFASIH 132 D +F ++Q +I+R AP ++VT N G F LD+ L + LDF S+DSYP F + Sbjct: 216 DQVRAFNRLQVEILRAHAPGKFVTHNFMGFFTDLDAFALAQD-LDFASWDSYPLGFTDLM 274 Query: 133 TDPEETEPLRDRSWSLSLSAV-----RSISPN-FCIMEQQSGPGGWVNRMNMPSPKPGQM 186 P E + R+ ++A R + F +MEQQ GP W + PSP PG + Sbjct: 275 PLPPEEKLRYARTGHPDVAAFHHDLYRGVGRGRFWVMEQQPGPVNWAP--HNPSPAPGMV 332 Query: 187 RLWTYQSIAHGADKLLYFR 205 RLWT++++AHGA+ + YFR Sbjct: 333 RLWTWEALAHGAEVVSYFR 351 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.133 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,339,498,268 Number of extensions: 49157325 Number of successful extensions: 85369 Number of sequences better than 1.0e-05: 340 Number of HSP's gapped: 84622 Number of HSP's successfully gapped: 340 Length of query: 222 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 91 Effective length of database: 1,826,543,238 Effective search space: 166215434658 Effective search space used: 166215434658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)