BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0008.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D604.1 B1D604_9BACL Transcriptional regulator, AraC family 198 4e-49 B3K6X6.1 B3K6X6_9BACI Helix-turn-helix-domain containing protein... 145 3e-33 B1DAD1.1 B1DAD1_9BACL Transcriptional regulator, AraC family 141 6e-32 B1D8G6.1 B1D8G6_9BACL Transcriptional regulator, AraC family 108 7e-22 B0MR70.1 B0MR70_9FIRM Putative uncharacterized protein 97 2e-18 >B1D604.1 B1D604_9BACL Transcriptional regulator, AraC family Length = 281 Score = 198 bits (504), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 108/277 (38%), Positives = 155/277 (55%), Gaps = 6/277 (2%) Query: 1 MNKQCYLPTPAYTKYVCYPEFLGHYSNFPQHAERRS-----EGLLNSYNLHLIFAGEGYV 55 M + +LP P + +++C+P+F+G YS FP+HA R L YNLH++ G+GY+ Sbjct: 1 MKEHIFLPKPIFPRHICFPDFIGGYSEFPEHAVNREFATTENNLDRYYNLHIVLGGKGYL 60 Query: 56 FHEGERIPMGRGKGFLFPRGAYQQYGSDLSRPWDVRWVHFGAGLPLPLLEEVDQSRGYFF 115 + + +G+GFL+ G Q Y SD PW +RWVHF P L+ + F Sbjct: 61 HTDNMTYELTQGQGFLYGPGLRQTYHSDARDPWSIRWVHFYGARPEEFLDGKGLDEPWLF 120 Query: 116 TFDPEAGLESLFEEMYRLGVSYETRYEPRLSALLYEILITLLQNSEPQHGAVSLEIRHSI 175 A +E+L E + LG SYE E ++ LYE+L T +Q + Q + I Sbjct: 121 HLPDLAPVEALMERLLELGRSYEVDDEYAAASTLYELL-TRIQTTASQLNVSVHQTADKI 179 Query: 176 RNAANIIHRECAGAWTLESMARLSGYSSYHFLRLFRLVMGKTPNRFLTDCRLARAKLLLV 235 R AAN + C +TLE A ++GYSS++F R F GK+ + FLT+ R+ RAK LL Sbjct: 180 RAAANYVRANCNQPFTLEQAAAIAGYSSHYFSRKFNQTFGKSFSDFLTEARILRAKQLLA 239 Query: 236 STGLTVAEIARQAGFAQSSYFIKVFREFEGMPPNQYR 272 ST L+V IA + GF+Q+SYF K FR+ EGM P Q+R Sbjct: 240 STRLSVKLIALETGFSQTSYFSKCFRDQEGMTPLQFR 276 >B3K6X6.1 B3K6X6_9BACI Helix-turn-helix-domain containing protein AraC type Length = 278 Score = 145 bits (367), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 98/284 (34%), Positives = 142/284 (50%), Gaps = 13/284 (4%) Query: 1 MNKQCYLPTPAYTKYVCYPEFLGHYSNFPQHAERRSEGLLNSYNLHLIFAGEGYVFHEGE 60 M +Q +L T + C PE +G Y + P H R+ G LN++N+H + G+G+V + Sbjct: 1 MKRQSHLLTLPQMPFFCLPESVGIYRDEPDHTVTRAAGSLNNFNIHYVATGKGHVEIDNV 60 Query: 61 RIPMGRGKGFL-FPRGAYQQYGSDLSRPWDVRWVHF-GAGLPLPLLEEVDQSRGY----F 114 +G G+ L FP A Q Y S PWDVRW HF G+GL +E RG+ Sbjct: 61 VHTLGPGEAVLYFPMQA-QHYYSSEDDPWDVRWFHFYGSGLQNYFIE-----RGFHKSQL 114 Query: 115 FTFDPEAGLESLFEEMYRLGVSYETRYEPRLSALLYEILITLLQNSEPQHGAVSLEIRHS 174 ++ A E E + ++ +LS L Y +L ++ + S + Sbjct: 115 WSIRQPAVFEEAHEALLNEAETHRMLKPAQLSTLTYSLLAVFVEQASSLSDNKSNNSANR 174 Query: 175 IRNAANIIHRECAGAWTLESMARLSGYSSYHFLRLFRLVMGKTPNRFLTDCRLARAK-LL 233 I I+ +E A + LE A G S+Y+F +LFR VM TP F+T CRL AK L Sbjct: 175 IHELLPIMQQEAAQPFILEEWAARLGVSTYYFCKLFRNVMEMTPMDFVTRCRLQMAKQWL 234 Query: 234 LVSTGLTVAEIARQAGFAQSSYFIKVFREFEGMPPNQYRRVFGI 277 L T+ +IA +AG+ SYF K F E EGM P+ YRR++G+ Sbjct: 235 LDHKDKTIGQIAVEAGYPSVSYFNKRFMEHEGMTPSSYRRLYGV 278 >B1DAD1.1 B1DAD1_9BACL Transcriptional regulator, AraC family Length = 279 Score = 141 bits (356), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 92/280 (32%), Positives = 135/280 (48%), Gaps = 4/280 (1%) Query: 1 MNKQCYLPTPAYTKYVCYPEFLGHYSNFPQHAERRSEGLLNSYNLHLIFAGEGYVFHEGE 60 M +Q L T Y C PE +G+Y P+H+ R G LN++N+H +G+GY +G Sbjct: 1 MKRQSLLLTLPNMPYFCMPESVGNYRFDPEHSTTREAGALNNFNIHYCVSGKGYAEIDGV 60 Query: 61 RIPMGRGKGFLFPRGAYQQYGSDLSRPWDVRWVHF--GAGLPLPLLEEVDQSRGYFFTFD 118 + G+ L+ Q+Y S PWDVRW HF G GL L+E+ + Sbjct: 61 VHELRAGEAILYFPLQKQRYYSSQDEPWDVRWFHFYGGTGLRDYLIEQGFHKSPLWKLRQ 120 Query: 119 PEAGLESLFEEMYRLGVSYETRYEPRLSALLYEILITLLQNSEPQHGAVSLEIRHSIRNA 178 P+ E EE+ R ++ +LS L Y +L ++++ G + I Sbjct: 121 PQI-WEQAHEELLREAETHRILRPAQLSMLTYMLLTVFVEHASALQGNKTGTSSERIMEL 179 Query: 179 ANIIHRECAGAWTLESMARLSGYSSYHFLRLFRLVMGKTPNRFLTDCRLARAK-LLLVST 237 ++ +E + LE A G S Y+F +LFR TP F+T CRL AK LL Sbjct: 180 LPLMQQEAVKPFILEEWAERVGVSPYYFCKLFRNATRMTPMDFITRCRLQAAKQWLLERK 239 Query: 238 GLTVAEIARQAGFAQSSYFIKVFREFEGMPPNQYRRVFGI 277 + +IA +AG+ SYF K F E EGM P YRR++G+ Sbjct: 240 DDNIGQIAEEAGYPSVSYFNKRFMEHEGMTPTAYRRLYGV 279 >B1D8G6.1 B1D8G6_9BACL Transcriptional regulator, AraC family Length = 289 Score = 108 bits (269), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 73/232 (31%), Positives = 110/232 (47%), Gaps = 5/232 (2%) Query: 46 HLIFA----GEGYVFHEGERIPMGRGKGFLFPRGAYQQYG-SDLSRPWDVRWVHFGAGLP 100 H+IF G GY+ +E + IP+ RG GFL + +Y PW+V W++ Sbjct: 44 HVIFQYTLNGLGYIEYEQQCIPLPRGTGFLVKVPSKHRYFYKSEDEPWEVLWLNLSGDEA 103 Query: 101 LPLLEEVDQSRGYFFTFDPEAGLESLFEEMYRLGVSYETRYEPRLSALLYEILITLLQNS 160 + + V G DP + + F ++ R + + +LSA +YE +I L+Q S Sbjct: 104 SRIWDLVIAQEGPIIRRDPNSPIIHAFRKLLRSIAEEKITDKYQLSAKVYEWMIALVQTS 163 Query: 161 EPQHGAVSLEIRHSIRNAANIIHRECAGAWTLESMARLSGYSSYHFLRLFRLVMGKTPNR 220 +S I+ A + + + A TL+ +A G + YH RLF+ +P Sbjct: 164 REVSREISTSSSSLIQRAKDFMKQHYAQPLTLDQLAEHCGVNKYHLCRLFQKSDQTSPLA 223 Query: 221 FLTDCRLARAKLLLVSTGLTVAEIARQAGFAQSSYFIKVFREFEGMPPNQYR 272 +L D R+ A LL +T L V I GF SSYF KVFRE+ M P +YR Sbjct: 224 YLRDRRMEAAVALLRTTELPVQTIGEMCGFENSSYFGKVFREYMSMTPKEYR 275 >B0MR70.1 B0MR70_9FIRM Putative uncharacterized protein Length = 290 Score = 96.7 bits (239), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 65/226 (28%), Positives = 101/226 (44%), Gaps = 5/226 (2%) Query: 51 GEGYVFHEGERIPMGRGKGFLFPRGAYQQYGSDLSRPWDVRWVHFGAGLPLPLLEEVDQS 110 G G + GE+ + F P G +Y S WD W+ +L+ + Sbjct: 64 GSGVLIMNGEKHIINPYTAFFIPAGCPHEYYST-DESWDTHWIKPSGKSCTDMLKALGFD 122 Query: 111 RGYFFTFDPE--AGLESLFEEMYRLGVSYETRYEPRLSALLYEILITLLQNSEPQHGAVS 168 + F E L+ F M+ +S + R S LY I L + + G+V Sbjct: 123 KPKIFPLPKEEITYLDRCFRCMHEALLSDKVDGNFRASGYLYSFFIELSRLAAKGKGSV- 181 Query: 169 LEIRHSIRNAANIIHRECAGAWTLESMARLSGYSSYHFLRLFRLVMGKTPNRFLTDCRLA 228 +I ++ A I LES+++ +G SS H RLFR + P ++T ++ Sbjct: 182 -QIAPAVTKAIAYIDENYMKQPGLESISKAAGVSSQHLCRLFRQTLNCRPMEYITKRKIQ 240 Query: 229 RAKLLLVSTGLTVAEIARQAGFAQSSYFIKVFREFEGMPPNQYRRV 274 AK+LL T TV +IA + GF SSYF K+F+ FE + P+Q+R Sbjct: 241 AAKMLLAETRKTVEQIAEETGFCDSSYFCKIFKRFESITPSQFRNA 286 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.141 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,735,018,068 Number of extensions: 73858965 Number of successful extensions: 223101 Number of sequences better than 1.0e-05: 3334 Number of HSP's gapped: 223171 Number of HSP's successfully gapped: 3336 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 130 (54.7 bits)