BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0004.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K564.1 B3K564_9BACI Efflux transporter, RND family, MFP subunit 331 8e-89 B7DDJ6.1 B7DDJ6_DESSA Putative PAS/PAC sensor protein 69 5e-10 Q5X0V5.1 Q5X0V5_LEGPA Putative uncharacterized protein 64 3e-08 Q5ZRF8.1 Q5ZRF8_LEGPH Lipoprotein 63 4e-08 A1HTE3.1 A1HTE3_9FIRM Efflux transporter, RND family, MFP subunit 63 6e-08 >B3K564.1 B3K564_9BACI Efflux transporter, RND family, MFP subunit Length = 362 Score = 331 bits (848), Expect = 8e-89, Method: Compositional matrix adjust. Identities = 169/346 (48%), Positives = 241/346 (69%), Gaps = 3/346 (0%) Query: 14 GYFIYDKMSKPKEQLQPA-EPAQVISFPVTKESMTKSIQIKGTSKYEHETLVYAPFASKV 72 GY+ Y ++ KPKE P E QVISFPVT+E++ ++Q+KGTS+Y ET VYAPF +KV Sbjct: 19 GYWGYGQV-KPKEPEIPMMEEPQVISFPVTEETILSTVQVKGTSEYGKETNVYAPFEAKV 77 Query: 73 TEWKVENGGQVKKGAVMYTLDTETLKNEIATQEATIRKAKLEAELNAFVSQQTEESAAVG 132 W VENG Q+KKG ++ L+ + ++ ++ +EA ++K KLE EL F Q ++A VG Sbjct: 78 ESWNVENGQQIKKGDALFKLEQKAIRQQLQQKEAELQKRKLEQELKEFTLNQDLDNAPVG 137 Query: 133 ATEAERLKALATQETSRIGDDLNQVNAEIQAREIADKKKKIQSASYHAPSSGIFLFDNAS 192 ATEAERLK LA QE +R+ L++VN +I+ + +AD +++ ++Y +P++G+FLFD Sbjct: 138 ATEAERLKMLADQENARLSSQLDEVNNDIERQTLADLNDRLRQSTYVSPAAGLFLFDPTQ 197 Query: 193 EIPRMVTDNQYLGKIVDLSKLQFIALVGEQDVFNIKPGMKVKVKMTASKDVKLTGVVKKV 252 + P+ VT NQY+GKIVDL L+ +A VGE D+F+I+P M V+VK+ + KD+KL G V +V Sbjct: 198 QRPQSVTANQYIGKIVDLDSLRLVAYVGENDIFSIQPDMAVEVKLPSIKDLKLKGKVLEV 257 Query: 253 SKFXXXXXXXXXXXXXXPQFEVVISLEPNELLIGGLSLTGEIETLRKDNVTAVSNIAIIH 312 +KF PQF+V+ISL N+ LIGGL+L GEI T RK+ V +A++ Sbjct: 258 AKF-AEATAQEKQTSQTPQFKVIISLPKNDRLIGGLTLNGEIVTKRKNKAVVVPKLAVMT 316 Query: 313 EGDLSYVMLDKGNGQTEKVQVKTGLETTDKVEILSGLKPGDTVVLQ 358 EGD SYVM+DKG GQ E+ ++TG++T DK+E+LSGLK GDTVVLQ Sbjct: 317 EGDSSYVMVDKGGGQIERQDIETGMQTLDKIEVLSGLKAGDTVVLQ 362 >B7DDJ6.1 B7DDJ6_DESSA Putative PAS/PAC sensor protein Length = 583 Score = 69.3 bits (168), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 103/382 (26%), Positives = 164/382 (42%), Gaps = 71/382 (18%) Query: 38 SFPVTKESMTKSIQIKGTSKYEHETLVYAPFASKVTEWKVENGGQVKKGAVMYTLDT--- 94 S VT ++SI + G E + APF +T+ G ++ + + TLDT Sbjct: 208 SMVVTPRPFSRSISLAGVVAPLEELTLVAPFNGIITKTDFFYGERIPRNQTIITLDTSEI 267 Query: 95 ---------ETLKNEIATQEAT-------IRKAKLEAE-----LNAFVSQQTEESAAVGA 133 E +K+ E T + KA+ E E LN+ +TEE + Sbjct: 268 ASKMRSAFTEYIKSRKKYYELTNWKNTSEVLKARRELEQARRTLNS-SKAKTEEDKMLFE 326 Query: 134 ----------TEAERLK-----ALATQETSRIGDDLNQVN---AEIQAREIADK------ 169 T + LK +++QET R D LN+ + EI E+A+ Sbjct: 327 KGIIPRNQYDTSFQELKNNESRLVSSQETYR--DVLNKGDHEYVEIARMELANAETNYNT 384 Query: 170 -KKKIQSASYHAPSSGIFLFDN-----ASEIPR--MVTDNQYLGKIVDLSKLQFIALVGE 221 K+K+ ++ AP SG+ L N A EI VT+ Q L I L L A V E Sbjct: 385 LKEKMSRSTVTAPVSGVALRPNSKSGDAKEITSGMSVTEGQPLLSIASLEGLSISAKVDE 444 Query: 222 QDVFNIKPGMKVKVKMTASKDVKLTGVVKKVSKFXXXXXXXXXXXXXXPQFEVVISLE-- 279 ++ +++ G V V A D KL G + +S P FE I L Sbjct: 445 LNINSLQLGQPVTVTGDAFPDHKLKGEIAMISS-------QAGGEGKVPTFETTIRLPHL 497 Query: 280 PNEL---LIGGLSLTGEIETLRKDNVTAVSNIAIIHEGDLSYVMLDKGNGQTEKVQVKTG 336 P ++ + G++ ++ET +N V AI +G ++ + + +G +V V+TG Sbjct: 498 PKDVSKNVRIGMTANMQVETYSNENAIMVPFSAINRDGSKVFLRVREKDGTVREVDVETG 557 Query: 337 LETTDKVEILSGLKPGDTVVLQ 358 T ++VEILSG++PG T++L+ Sbjct: 558 YTTINEVEILSGIEPGTTILLK 579 >Q5X0V5.1 Q5X0V5_LEGPA Putative uncharacterized protein Length = 381 Score = 63.9 bits (154), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 88/352 (25%), Positives = 144/352 (40%), Gaps = 38/352 (10%) Query: 38 SFPVTKESMTKSIQIKGTSKYEHETLVYAPFASKVTEWKVENGGQVKKGAVMYTLDTETL 97 ++ V + + K++ GT + E+ + +P + V G VKKG ++ TL++ L Sbjct: 33 TYEVKPQPLHKTLHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNEL 92 Query: 98 KNEI---------ATQEATIRKAKL---EAELNA-FVSQQTEESAAVGATEAERLKALAT 144 + + A +I KAK E NA +S+ S G A AT Sbjct: 93 QKQYNDTLTDYLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQAT 152 Query: 145 QETSRI---GDDLNQVNAEIQAREIADKKKKIQSASYH-----APSSGIFLF-------- 188 ++ S + DD N N + DK +KI +++++ APS GI L+ Sbjct: 153 RKLSEMLEKMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDK 212 Query: 189 DNASEIPRMVTDNQYLGKIVDLSKLQFIALVGEQDVFNIKPGMKVKVKMTASKDVKLTGV 248 +N + + Q + I DL + V E D+ I+PGM+ + A +L G Sbjct: 213 NNRVTVGSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMQATISGVAFGRHQLKGK 272 Query: 249 VKKVSKFXXXXXXXXXXXXXXPQFEVVI---SLEPNELLIGGLSLTGEIE-TLRKDNVTA 304 + V+ P F V+ SL P + + ++ IE + +N Sbjct: 273 LVAVN-----AQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLL 327 Query: 305 VSNIAIIHEGDLSYVMLDKGNGQTEKVQVKTGLETTDKVEILSGLKPGDTVV 356 + A+ E S V + G+ EK + TG D V + SGLK GD VV Sbjct: 328 IPISAVKREKGSSIVQVQLAKGKVEKRMITTGAAQADSVVVESGLKSGDVVV 379 >Q5ZRF8.1 Q5ZRF8_LEGPH Lipoprotein Length = 381 Score = 63.2 bits (152), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 87/352 (24%), Positives = 144/352 (40%), Gaps = 38/352 (10%) Query: 38 SFPVTKESMTKSIQIKGTSKYEHETLVYAPFASKVTEWKVENGGQVKKGAVMYTLDTETL 97 ++ V + + K++ GT + E+ + +P + V G VKKG ++ TL++ L Sbjct: 33 TYEVKPQPLHKTLHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNEL 92 Query: 98 KNEI---------ATQEATIRKAKL---EAELNA-FVSQQTEESAAVGATEAERLKALAT 144 + + A +I KAK E NA +S+ S G A AT Sbjct: 93 QKQYNDTLTDYLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQAT 152 Query: 145 QETSRI---GDDLNQVNAEIQAREIADKKKKIQSASYH-----APSSGIFLF-------- 188 ++ S + DD N N + DK +KI +++++ APS GI L+ Sbjct: 153 RKLSEMLEKMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDK 212 Query: 189 DNASEIPRMVTDNQYLGKIVDLSKLQFIALVGEQDVFNIKPGMKVKVKMTASKDVKLTGV 248 +N + + Q + I DL + V E D+ I+PGM+ + A +L G Sbjct: 213 NNRVTVGSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMEATISGVAFGRHQLKGK 272 Query: 249 VKKVSKFXXXXXXXXXXXXXXPQFEVVI---SLEPNELLIGGLSLTGEIE-TLRKDNVTA 304 + V+ P F V+ SL P + + ++ IE + +N Sbjct: 273 LVAVN-----AQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLL 327 Query: 305 VSNIAIIHEGDLSYVMLDKGNGQTEKVQVKTGLETTDKVEILSGLKPGDTVV 356 + A+ E S V + G+ +K + TG D V + SGLK GD VV Sbjct: 328 IPIAAVKREKGSSVVQVQLAKGKVQKRMITTGAAQADSVVVESGLKSGDVVV 379 >A1HTE3.1 A1HTE3_9FIRM Efflux transporter, RND family, MFP subunit Length = 372 Score = 62.8 bits (151), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 84/335 (25%), Positives = 141/335 (42%), Gaps = 33/335 (9%) Query: 27 QLQPAEPAQVISFPVTKESMTKSIQIKGTSKYEHETLVYAPFASKVTEWKVENGGQVKKG 86 ++ A P QVI V + + ++ GT K + + + +++ E KV+ QVK G Sbjct: 37 KMSAAAPQQVIQ--VERGDIVATVAATGTIKPVNMVDISSKITAQIKEVKVKENDQVKAG 94 Query: 87 AVMYTLDTETLKNEIATQEATIRKAKLEAELNAFVSQQTEESAAVGATEAERLKALATQE 146 V+ L+ L+ ++ + + A ++ E A ++ A+G E+L + Sbjct: 95 QVLVILEDSGLQAQVTQAKERLDNAAVKYERIARLT-------AIGGASKEQL------D 141 Query: 147 TSRIGDDLNQVNAEIQAREIADKKKKIQSASYHAPSSGIFL---FDNASEIPRMVTDNQY 203 T+R+ D N A + K+ A +P G + + + V + Sbjct: 142 TARM--DYNIAKASYD-----EVMSKLSEAVIVSPIDGTVIGKPLSAGEMVTQGVNNPTV 194 Query: 204 LGKIVDLSKLQFIALVGEQDVFNIKPGMKVKVKMTASKDVKLTGVVKKVSKFXXXXXXXX 263 + + D+SK+Q A V + D+ + G KV + A + TG V +SK Sbjct: 195 ILTVADMSKMQIEAQVDQTDIGKVALGQKVTFTVDAYPGKEFTGTVATISK-------KP 247 Query: 264 XXXXXXPQFEVVISLEPNE-LLIGGLSLTGEIETLRKDNVTAVSNIAIIHEGDLSYVMLD 322 + V I + E LL G+ I + V + AI + YV++ Sbjct: 248 VVQQNVTYYPVTIDVNNAENLLNPGMVARVSITVSERKGVVTLPLSAIRTDKTGKYVIVI 307 Query: 323 KGNGQTEKVQVKTGLETTDKVEILSGLKPGDTVVL 357 NGQT+ V V TG D+VEI SGLK GD VV+ Sbjct: 308 GDNGQTQNVPVTTGSVGDDRVEITSGLKEGDKVVV 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.310 0.128 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,755,501,057 Number of extensions: 62644705 Number of successful extensions: 192243 Number of sequences better than 1.0e-05: 522 Number of HSP's gapped: 192819 Number of HSP's successfully gapped: 522 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)