BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0001.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZFR0.1 C0ZFR0_BREBN Conserved hypothetical membrane protein 340 8e-92 Q65MU4.1 Q65MU4_BACLD YeaB 335 2e-90 A8FAH1.1 A8FAH1_BACP2 CDF family cation diffusion facilitator 332 2e-89 B3KDK8.1 B3KDK8_9BACI Cation diffusion facilitator family transp... 331 5e-89 B1DE11.1 B1DE11_9BACL Cation diffusion facilitator family transp... 327 9e-88 >C0ZFR0.1 C0ZFR0_BREBN Conserved hypothetical membrane protein Length = 293 Score = 340 bits (872), Expect = 8e-92, Method: Compositional matrix adjust. Identities = 157/277 (56%), Positives = 208/277 (75%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 ++ YSD+KQ E+GAW+S+F YI S +K+ + + S+AL+ADGLNNSTD++AS+A+L G Sbjct: 1 MDVYSDLKQGERGAWVSIFAYIFCSVLKIGVAYVTASEALMADGLNNSTDVVASIAVLIG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 L+I+R+PPD++H YGHFRAET++ALVASFIM+ VG+QV+ V P PDL+A W Sbjct: 61 LRIARKPPDNDHPYGHFRAETISALVASFIMLFVGVQVITNAVPSLFDPKNSAPDLLAGW 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 TA AV+M VY YN +LA K NS A+ A A DNRSDA VSIG F G+ GAQF L WLD Sbjct: 121 TALATAVIMLFVYRYNKKLAIKTNSQALDAAAADNRSDAFVSIGTFIGVAGAQFQLHWLD 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 PL +VGL+I KTAW+IFR+ATH LTDGFD ++L ++ T++ I GV I DIKAR HG Sbjct: 181 PLAAFVVGLIILKTAWDIFREATHRLTDGFDESDLADLRATISSISGVRDISDIKARYHG 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 ++VLVD + VD LNVV+SH ITE+IE+Q++ H++ Sbjct: 241 SSVLVDVVIHVDPGLNVVESHTITEKIEEQMRKIHRI 277 >Q65MU4.1 Q65MU4_BACLD YeaB Length = 290 Score = 335 bits (860), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 153/277 (55%), Positives = 208/277 (75%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 +E Y ++K+ E GAW+S+ Y++LSAVKL +G A S+AL ADGLNN+TDIIASLA+L G Sbjct: 1 MERYDELKKGETGAWVSIIAYLVLSAVKLAVGLAFHSEALRADGLNNTTDIIASLAVLIG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 L+IS++PPD +H YGHFRAET+A+++ASFIM+ VGL+VLY P TPD+IAAW Sbjct: 61 LRISQKPPDEDHPYGHFRAETIASMIASFIMMVVGLEVLYSSGEMLFNPKETTPDMIAAW 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 TAAG AVVMF V++YN RLA+K+NS+A+ A A DN+SDA VSIG F GI +QF L W+D Sbjct: 121 TAAGSAVVMFLVFLYNKRLAKKVNSHALYAAAADNKSDAYVSIGTFAGIFASQFNLAWVD 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 I+GL+ICKTAW IF++A H LTDGFD + K+T+ ++ GV +IKD+KAR G Sbjct: 181 TAAAFIIGLIICKTAWGIFKEAAHSLTDGFDIKHISTYKETIRKVPGVGNIKDVKARYLG 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 + + V+ V V S+LN+ +SHDI +EIE ++++ H + Sbjct: 241 SAIHVEIIVEVRSDLNIAESHDIADEIERRMQEEHSI 277 >A8FAH1.1 A8FAH1_BACP2 CDF family cation diffusion facilitator Length = 299 Score = 332 bits (852), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 154/277 (55%), Positives = 204/277 (73%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 +E Y++++Q E GAW+S+ Y++LSAVKLLIG S+AL ADGLNN+TDIIASLA+L G Sbjct: 1 MERYNELRQGETGAWVSIIAYVILSAVKLLIGYTFHSEALSADGLNNTTDIIASLAVLIG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 L+IS++PPD +H YGHFRAE +A+LVASFIM+ VGLQVL +TPD+IAAW Sbjct: 61 LRISQKPPDEDHPYGHFRAENIASLVASFIMMLVGLQVLLSAGQSLFSSEHQTPDMIAAW 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 TAAG AVVM+ VY+YN L++++NS A+ A A DN+SDA VSIG F GII +QF L W+D Sbjct: 121 TAAGSAVVMYGVYIYNRNLSKRINSQALHAAAADNKSDAYVSIGTFVGIIASQFQLAWID 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 L ++GL+ICKTAWEIFR A+H LTDGF ++ K+T+ GV +KDIKAR G Sbjct: 181 TLAAFVIGLIICKTAWEIFRDASHSLTDGFHIKDMSKYKETIEATPGVGDLKDIKARYLG 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 + + VD V V+ +LN+ +SHDI +EIE ++K H + Sbjct: 241 STIHVDVVVEVEPHLNIAESHDIADEIERRMKKEHDI 277 >B3KDK8.1 B3KDK8_9BACI Cation diffusion facilitator family transporter Length = 290 Score = 331 bits (849), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 154/277 (55%), Positives = 207/277 (74%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 + Y +IKQ E+GAW+S+ Y++LS+ K+ G S ALLADG NN TDI+ASLA+L G Sbjct: 1 MNAYDNIKQGERGAWISIAAYLVLSSFKIFCGYLFASSALLADGFNNLTDIVASLAVLIG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 L+IS++PPDS+H+YGH RAET+AALVASFIM VG+QVL + V F + + E P+L +A Sbjct: 61 LRISQKPPDSDHAYGHLRAETIAALVASFIMAVVGIQVLVEAVRSFFEGSKEVPNLWSAG 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 A CAV M VY YN LAR++++ A++A A+DN SDALVS+GA GIIGAQFGLPWLD Sbjct: 121 VAGICAVAMLGVYRYNRNLARRIDNQALMAAAKDNLSDALVSVGAAVGIIGAQFGLPWLD 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 + + VG++ICKTAWEIFR T+ LTDGFD N L ++ T+A GVE IKD+KARIHG Sbjct: 181 TVAAVAVGVIICKTAWEIFRDCTYSLTDGFDENRLSDLRSTIARTPGVEGIKDMKARIHG 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 N+VLVD + VD +++V++ H I++ IE+Q++ H + Sbjct: 241 NHVLVDVVIEVDPDISVLEGHQISDRIEEQMEKIHNI 277 >B1DE11.1 B1DE11_9BACL Cation diffusion facilitator family transporter Length = 291 Score = 327 bits (837), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 145/276 (52%), Positives = 213/276 (77%) Query: 2 ENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAGL 61 + Y ++K E+GA +S+ YI LSA+KL+IG SGS+AL ADGLNN+TDI+AS+A+L GL Sbjct: 5 QRYENLKMGERGAIVSIIAYICLSALKLIIGNISGSEALKADGLNNATDIVASIAVLIGL 64 Query: 62 KISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAWT 121 K++++P D +H YGH+++ET+A+L+ASFIM+AVG++VL V+ + + ++PD IA WT Sbjct: 65 KLAQKPADEDHPYGHWKSETIASLIASFIMMAVGIEVLINAVSSVFRGSEQSPDFIAVWT 124 Query: 122 AAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLDP 181 CA VM+ VY YNI LARK+ S+A++A A+DN SDALVSIGA GI+G+QF LPWLDP Sbjct: 125 GLFCAAVMYVVYRYNINLARKIKSHAVMAAAKDNISDALVSIGAVIGIVGSQFKLPWLDP 184 Query: 182 LTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHGN 241 +T + VGL+ICKTAW+IFR+A+H L+DGFD L+ K+ +++GVE +KD+KAR +G+ Sbjct: 185 VTAVAVGLIICKTAWDIFREASHHLSDGFDEAALQDFKEKAKQVEGVEDVKDLKARNYGS 244 Query: 242 NVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 N ++D + +D NL+ ++HD+ E+E++LK +V Sbjct: 245 NAVIDVVLSIDKNLDFHKAHDVATEVENELKKTSEV 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,574,286,143 Number of extensions: 59145895 Number of successful extensions: 176735 Number of sequences better than 1.0e-05: 2662 Number of HSP's gapped: 174159 Number of HSP's successfully gapped: 2664 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)