BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0587.0016.p (262 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P39135.2 SFP_BACSU 4'-phosphopantetheinyl transferase sfp EC=2.7... 167 1e-39 B4AIW4.1 B4AIW4_BACPU 4'-phosphopantetheinyl transferase sfp (Su... 166 1e-39 A8HTT2.1 A8HTT2_BACSU Sfp 166 2e-39 A8HTS6.1 A8HTS6_BACSU Sfp 166 2e-39 A5N8D4.1 A5N8D4_CLOK5 Predicted phosphopantetheinyl transferase 166 3e-39 >P39135.2 SFP_BACSU 4'-phosphopantetheinyl transferase sfp EC=2.7.8.- Length = 224 Score = 167 bits (422), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 86/208 (41%), Positives = 126/208 (60%), Gaps = 5/208 (2%) Query: 44 LLSCVSREKSEKLSRFHHQEDLIRGLIGDLLVRKIISETFAVPVKGIVFDTNSYGKPCLA 103 +S +S EK EK RF+H+ED R L+GD+LVR +IS + + I F T YGKPC Sbjct: 20 FMSFISPEKREKCRRFYHKEDAHRTLLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPC-- 77 Query: 104 IPDNSSFHYNVSHSGDWVVCAIDDCPVGIDIEKIQPVQLEISRRFFAREEVEFIEKAPSD 163 IPD H+N+SHSG WV+CA D P+GIDIEK +P+ LEI++RFF++ E + D Sbjct: 78 IPDLPDAHFNISHSGRWVICAFDSQPIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKD 137 Query: 164 EQRQKRFFAVWTAKESYIKAIGQGLSHSLNNFSTV--REGSVEGWRVFDHCNWYLKAYNL 221 EQ F+ +W+ KES+IK G+GLS L++FS ++G V H Y+K Y + Sbjct: 138 EQTD-YFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEV 196 Query: 222 DDRYALAVCGQRSQVCDTVRVISPDDII 249 D Y +AVC + + ++S ++++ Sbjct: 197 DPGYKMAVCAAHPDFPEDITMVSYEELL 224 >B4AIW4.1 B4AIW4_BACPU 4'-phosphopantetheinyl transferase sfp (Surfactinsynthetase-activating enzyme) EC=2.7.8.- Length = 230 Score = 166 bits (421), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 90/231 (38%), Positives = 144/231 (62%), Gaps = 11/231 (4%) Query: 26 LQIYAVAISHFPN----TQLLPLLSCVSREKSEKLSRFHHQEDLIRGLIGDLLVRKIISE 81 ++I+A+ + H + Q+ L VS EK RF D R L+G++LVR++I + Sbjct: 1 MKIFAIQLQHLDDIHARKQIDQLKPFVSAEKRAAAERFRFLIDARRTLLGEVLVRQMIHD 60 Query: 82 TFAVPVKGIVFDTNSYGKPCLA-IPDNSSFHYNVSHSGDWVVCAIDDCPVGIDIEKIQPV 140 + +P IVF+T GKP + IP SFH+N+SHSGDWVVCAIDD PVGIDIE+I+P+ Sbjct: 61 MYDLPFDEIVFETEGNGKPVVRHIP---SFHFNLSHSGDWVVCAIDDAPVGIDIEEIKPI 117 Query: 141 QLEISRRFFAREEVEFIEKAPSDEQRQKRFFAVWTAKESYIKAIGQGLSHSLNNFST-VR 199 L I++RFF+ +E E + P+ E+++ FF +W+ KE++IK G+GLS+ L++F+ + Sbjct: 118 DLAIAKRFFSADEYEDLLSQPA-ERQEAYFFHLWSMKEAFIKLTGKGLSYGLSSFTARLS 176 Query: 200 EGSVEGWRVFDH-CNWYLKAYNLDDRYALAVCGQRSQVCDTVRVISPDDII 249 E + DH +++ Y+LD Y +AVC ++ + V ++S D++ Sbjct: 177 EDGQATLALPDHEAPCFVQPYSLDPTYQMAVCTRKPSAAERVEILSCSDVL 227 >A8HTT2.1 A8HTT2_BACSU Sfp Length = 224 Score = 166 bits (420), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 86/208 (41%), Positives = 126/208 (60%), Gaps = 5/208 (2%) Query: 44 LLSCVSREKSEKLSRFHHQEDLIRGLIGDLLVRKIISETFAVPVKGIVFDTNSYGKPCLA 103 +S +S EK EK RF+H+ED R L+GD+LVR +IS + + I F T YGKPC Sbjct: 20 FMSFISPEKREKCRRFYHKEDAHRTLLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPC-- 77 Query: 104 IPDNSSFHYNVSHSGDWVVCAIDDCPVGIDIEKIQPVQLEISRRFFAREEVEFIEKAPSD 163 IPD H+N+SHSG WV+CA D P+GIDIEK +P+ LEI++RFF++ E + D Sbjct: 78 IPDLPDAHFNISHSGRWVICAFDSQPIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKD 137 Query: 164 EQRQKRFFAVWTAKESYIKAIGQGLSHSLNNFSTV--REGSVEGWRVFDHCNWYLKAYNL 221 EQ F+ +W+ KES+IK G+GLS L++FS ++G V H Y+K Y + Sbjct: 138 EQTD-YFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEV 196 Query: 222 DDRYALAVCGQRSQVCDTVRVISPDDII 249 D Y +AVC + + ++S ++++ Sbjct: 197 DPGYKMAVCAVHPDFPEDITMVSYEELL 224 >A8HTS6.1 A8HTS6_BACSU Sfp Length = 224 Score = 166 bits (420), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 86/208 (41%), Positives = 126/208 (60%), Gaps = 5/208 (2%) Query: 44 LLSCVSREKSEKLSRFHHQEDLIRGLIGDLLVRKIISETFAVPVKGIVFDTNSYGKPCLA 103 +S +S EK EK RF+H+ED R L+GD+LVR +IS + + I F T YGKPC Sbjct: 20 FMSFISPEKREKCRRFYHKEDAHRTLLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPC-- 77 Query: 104 IPDNSSFHYNVSHSGDWVVCAIDDCPVGIDIEKIQPVQLEISRRFFAREEVEFIEKAPSD 163 IPD H+N+SHSG WV+CA D P+GIDIEK +P+ LEI++RFF++ E + D Sbjct: 78 IPDLPDAHFNISHSGRWVICAFDSQPIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKD 137 Query: 164 EQRQKRFFAVWTAKESYIKAIGQGLSHSLNNFSTV--REGSVEGWRVFDHCNWYLKAYNL 221 EQ F+ +W+ KES+IK G+GLS L++FS ++G V H Y+K Y + Sbjct: 138 EQTD-YFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEV 196 Query: 222 DDRYALAVCGQRSQVCDTVRVISPDDII 249 D Y +AVC + + ++S ++++ Sbjct: 197 DPGYKMAVCAVHPDFPEDITMVSYEELL 224 >A5N8D4.1 A5N8D4_CLOK5 Predicted phosphopantetheinyl transferase Length = 232 Score = 166 bits (419), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 93/227 (40%), Positives = 136/227 (59%), Gaps = 23/227 (10%) Query: 25 VLQIYAVAISHFPN-TQLLPLLSCVSREKSEKLSRFHHQEDLIRGLIGDLLVRKIISETF 83 ++ IYA+ +S N +L L+S +S EK ++ +FH EDL RG++ ++LVR I+ + F Sbjct: 1 MVNIYAINLSGNMNCYELDELMSFISEEKLYRVKKFHRLEDLKRGVMSEILVRFILCKDF 60 Query: 84 AVPVKGIVFDTNSYGKPCLAIPDNSSFHYNVSHSGDWVVCAIDDCPVGIDIEKIQPVQLE 143 V K + N YGKP L+ P+ S H+NVSHSG W+VCA+ + PVGIDIE+I+P+ Sbjct: 61 HVRNKDLSITKNYYGKPLLSYPE--SIHFNVSHSGYWIVCAVHNLPVGIDIEQIKPIDFS 118 Query: 144 ISRRFFAREEVEFIEKAPSDE-QRQKRFFAVWTAKESYIKAIGQGLSHSLNNFS------ 196 I+ FF+ E E I SDE R F+ WT KESYIKA+G+GL +LN+F+ Sbjct: 119 IAEHFFSESEYESI--LTSDEGPRLPLFYEFWTLKESYIKAVGKGLYMALNSFNIKLCND 176 Query: 197 ---TVREGSVEGWRVFDHCNWYLKAYNLDDRYALAVCGQRSQVCDTV 240 REGS E ++Y K Y++D Y L+VC + + D++ Sbjct: 177 DIQVEREGSFE--------DYYFKQYDIDKNYKLSVCARTNNFSDSI 215 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,585,498,834 Number of extensions: 65215017 Number of successful extensions: 133290 Number of sequences better than 1.0e-05: 613 Number of HSP's gapped: 132587 Number of HSP's successfully gapped: 613 Length of query: 262 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 129 Effective length of database: 1,807,701,208 Effective search space: 233193455832 Effective search space used: 233193455832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)