BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0587.0015.p (98 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3GUD5.1 C3GUD5_BACTU Transcription antitermination protein nusG 86 1e-15 C2NS75.1 C2NS75_BACCE Transcription antitermination protein nusG 86 1e-15 C0ZGR2.1 C0ZGR2_BREBN Putative transcription antitermination pro... 85 2e-15 A7Z6E4.1 A7Z6E4_BACA2 Transcription antitermination protein nusG 80 4e-14 A6TKC4.1 A6TKC4_ALKMQ NusG antitermination factor 69 1e-10 >C3GUD5.1 C3GUD5_BACTU Transcription antitermination protein nusG Length = 179 Score = 85.9 bits (211), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/84 (46%), Positives = 65/84 (77%) Query: 5 DKEELYFKKIPEEEMSIILKLLNQEEEINFSKVYTIESKVYVESGPLKGLEGIIKKINKH 64 D +++YF +IP++E++ I++LLN I S+ Y SKV V+SGPLKG EGII++I+K Sbjct: 90 DDDDIYFLEIPDQEITPIIQLLNDRGTIECSEAYFEGSKVIVKSGPLKGREGIIRRIDKR 149 Query: 65 TRRAKVLVSLMGDQRIIELGIEFI 88 +RAK+L++ +GD ++I++G+EF+ Sbjct: 150 KKRAKLLLNFLGDTKMIDVGVEFL 173 >C2NS75.1 C2NS75_BACCE Transcription antitermination protein nusG Length = 179 Score = 85.9 bits (211), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/84 (46%), Positives = 65/84 (77%) Query: 5 DKEELYFKKIPEEEMSIILKLLNQEEEINFSKVYTIESKVYVESGPLKGLEGIIKKINKH 64 D +++YF +IP++E++ I++LLN I S+ Y SKV V+SGPLKG EGII++I+K Sbjct: 90 DDDDIYFLEIPDQEITPIIQLLNDRGTIECSEAYFEGSKVIVKSGPLKGREGIIRRIDKR 149 Query: 65 TRRAKVLVSLMGDQRIIELGIEFI 88 +RAK+L++ +GD ++I++G+EF+ Sbjct: 150 KKRAKLLLNFLGDTKMIDVGVEFL 173 >C0ZGR2.1 C0ZGR2_BREBN Putative transcription antitermination protein Length = 177 Score = 85.1 bits (209), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 42/82 (51%), Positives = 61/82 (74%) Query: 7 EELYFKKIPEEEMSIILKLLNQEEEINFSKVYTIESKVYVESGPLKGLEGIIKKINKHTR 66 E Y+ I E+E++ IL+L+ E +++SKVY SKV VESGPLKGLEGIIKK++KH Sbjct: 92 EGAYYSSIEEKEITPILQLMGNGEVVDYSKVYLENSKVIVESGPLKGLEGIIKKVDKHKN 151 Query: 67 RAKVLVSLMGDQRIIELGIEFI 88 RAK+ ++ MG ++ I++GIE + Sbjct: 152 RAKIFLTFMGVEKTIDVGIEIL 173 >A7Z6E4.1 A7Z6E4_BACA2 Transcription antitermination protein nusG Length = 176 Score = 80.5 bits (197), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 39/77 (50%), Positives = 57/77 (74%) Query: 10 YFKKIPEEEMSIILKLLNQEEEINFSKVYTIESKVYVESGPLKGLEGIIKKINKHTRRAK 69 YF I +EE+ I++L+ + + +++SKVY SKV V SGPLKG+EGIIKKI+K RRAK Sbjct: 95 YFTTIKDEEILPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAK 154 Query: 70 VLVSLMGDQRIIELGIE 86 + +S MG +++ +GIE Sbjct: 155 ICLSFMGLDKMVNVGIE 171 >A6TKC4.1 A6TKC4_ALKMQ NusG antitermination factor Length = 162 Score = 69.3 bits (168), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 40/77 (51%), Positives = 53/77 (68%), Gaps = 1/77 (1%) Query: 14 IPEEEMSIILKLLNQEEEINFSKVYTIE-SKVYVESGPLKGLEGIIKKINKHTRRAKVLV 72 IP EEM +L+L NQ E I SKV E +KV V SGPL G+EG I K++K RAKV + Sbjct: 83 IPLEEMQRVLRLCNQGELIGLSKVIVKEGNKVQVVSGPLLGMEGHIIKVDKRKGRAKVCL 142 Query: 73 SLMGDQRIIELGIEFIE 89 S++GD + ++LGIE I+ Sbjct: 143 SVLGDAKTVDLGIEIIK 159 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.138 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 516,536,842 Number of extensions: 17559692 Number of successful extensions: 43907 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 43919 Number of HSP's successfully gapped: 13 Length of query: 98 Length of database: 3,060,696,203 Length adjustment: 67 Effective length of query: 31 Effective length of database: 2,429,488,198 Effective search space: 75314134138 Effective search space used: 75314134138 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)