BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0072.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIB2.1 B3KIB2_9BACI Succinyl-CoA ligase [ADP-forming] subunit ... 456 e-127 B1DLV7.1 B1DLV7_9BACL Succinyl-CoA ligase [ADP-forming] subunit ... 444 e-123 Q9KA21.1 Q9KA21_BACHD Succinyl-CoA ligase [ADP-forming] subunit ... 395 e-108 C0ZFB2.1 C0ZFB2_BREBN Succinyl-CoA synthetase alpha chain EC=6.2... 386 e-105 Q5L0N6.1 Q5L0N6_GEOKA Succinyl-CoA ligase [ADP-forming] subunit ... 383 e-104 >B3KIB2.1 B3KIB2_9BACI Succinyl-CoA ligase [ADP-forming] subunit alpha EC=6.2.1.5 Length = 309 Score = 456 bits (1174), Expect = e-127, Method: Compositional matrix adjust. Identities = 229/309 (74%), Positives = 246/309 (79%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVSL 60 MSIL+DKNTKVITQGITG T LFH KGALDYGTQM ITLENG V L Sbjct: 1 MSILIDKNTKVITQGITGKTALFHAKGALDYGTQMVGGTSPGKGGTEVEITLENGETVKL 60 Query: 61 PVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSRF 120 PV++ IYVPPAFAADSI+EAVDA ++LVICITEGIPVLDMVKVSR+ Sbjct: 61 PVYNTVEEAKAKTGATASVIYVPPAFAADSILEAVDAEMDLVICITEGIPVLDMVKVSRY 120 Query: 121 MEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGIG 180 MEG+ T LIGPNCPGVITPGECKIGIMPGYIH PG+VGVVSRSGTLTYEAVHQLT RGIG Sbjct: 121 MEGKKTRLIGPNCPGVITPGECKIGIMPGYIHTPGHVGVVSRSGTLTYEAVHQLTARGIG 180 Query: 181 QSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPVV 240 QSSAVGIGGDPVKG+EFID+L+LFNEDP TKAV WIK NMTKPVV Sbjct: 181 QSSAVGIGGDPVKGTEFIDVLRLFNEDPNTKAVIMIGEIGGTAEEEAAEWIKANMTKPVV 240 Query: 241 GFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEER 300 GFIGG TAPPGKRMGHAGAIISGGKGTA EKIAVLE+CGIKVAPTPAEMG+TLV VLEE+ Sbjct: 241 GFIGGATAPPGKRMGHAGAIISGGKGTAKEKIAVLEACGIKVAPTPAEMGSTLVSVLEEK 300 Query: 301 GILNAFTTH 309 G+L + TTH Sbjct: 301 GLLESCTTH 309 >B1DLV7.1 B1DLV7_9BACL Succinyl-CoA ligase [ADP-forming] subunit alpha EC=6.2.1.5 Length = 313 Score = 444 bits (1142), Expect = e-123, Method: Compositional matrix adjust. Identities = 226/308 (73%), Positives = 243/308 (78%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVSL 60 MSILV+K+TKVITQGITG+TGLFH KGALDYGTQM +ITLENG+ VSL Sbjct: 1 MSILVNKDTKVITQGITGATGLFHAKGALDYGTQMVGGVTPGKGGTNVDITLENGNVVSL 60 Query: 61 PVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSRF 120 PVF+ IYVPPAFAADSIMEAVDAGL+LVICITEGIPVLDMVKV R+ Sbjct: 61 PVFNTVEQAVAATGATASVIYVPPAFAADSIMEAVDAGLDLVICITEGIPVLDMVKVKRY 120 Query: 121 MEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGIG 180 MEG T LIGPNCPGVITPGECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQL+ RGIG Sbjct: 121 MEGSKTTLIGPNCPGVITPGECKIGIMPGYIHTKGHVGVVSRSGTLTYEAVHQLSTRGIG 180 Query: 181 QSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPVV 240 QSSAVGIGGDPVKGSEFIDIL FNEDP T AV WIK NM KPVV Sbjct: 181 QSSAVGIGGDPVKGSEFIDILSRFNEDPDTYAVIMIGEIGGTAEEEAAEWIKANMKKPVV 240 Query: 241 GFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEER 300 GFIGG TAPPGKRMGHAGAIISGGKGTA+EKIA LE+CGIKVAPTP++MG+TLV VLEE+ Sbjct: 241 GFIGGATAPPGKRMGHAGAIISGGKGTAAEKIATLEACGIKVAPTPSDMGSTLVSVLEEK 300 Query: 301 GILNAFTT 308 G+L TT Sbjct: 301 GLLEKCTT 308 >Q9KA21.1 Q9KA21_BACHD Succinyl-CoA ligase [ADP-forming] subunit alpha EC=6.2.1.5 Length = 302 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 203/310 (65%), Positives = 231/310 (74%), Gaps = 11/310 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MSIL++K+TKVI QGITG+TGLFHTK A++YGT + + G+EV Sbjct: 1 MSILINKDTKVIVQGITGATGLFHTKQAVEYGTNIVGGVTPG----------KGGTEVEG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +PVFD IYVPPAFAAD+IMEAVDA L+L ICITEGIPV+DMVKV R Sbjct: 51 IPVFDTVSQAVEATGANASVIYVPPAFAADAIMEAVDAELDLAICITEGIPVMDMVKVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 +MEG+ T L+GPNCPGVITP ECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQL+ GI Sbjct: 111 YMEGKKTRLVGPNCPGVITPEECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLSTAGI 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPV G+ FID+LKLFNEDP T AV WIK NMTKPV Sbjct: 171 GQSTAVGIGGDPVNGTNFIDVLKLFNEDPDTYAVIMIGEIGGTAEEEAAEWIKANMTKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAPPGKRMGHAGAIISGGKGTA+EKI LESCG+KVA TP+ MG TL+ VL+E Sbjct: 231 VGFIGGQTAPPGKRMGHAGAIISGGKGTAAEKIKTLESCGVKVAETPSVMGETLISVLKE 290 Query: 300 RGILNAFTTH 309 +G+L TTH Sbjct: 291 QGLLEKCTTH 300 >C0ZFB2.1 C0ZFB2_BREBN Succinyl-CoA synthetase alpha chain EC=6.2.1.5 Length = 300 Score = 386 bits (991), Expect = e-105, Method: Compositional matrix adjust. Identities = 202/305 (66%), Positives = 233/305 (76%), Gaps = 11/305 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MSILV+KNTKVITQGITG+TGLFHT+GA++YGTQM + G+EV Sbjct: 1 MSILVNKNTKVITQGITGATGLFHTRGAVEYGTQMVGGVTPG----------KGGTEVDG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +P+F+ IYV P FAAD+IMEAVDA L+LVICITEGIPVLDMVKV R Sbjct: 51 IPIFNTVKEAVEATGATASVIYVAPPFAADAIMEAVDAELDLVICITEGIPVLDMVKVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 +MEG+ T+L+GPNCPGVITPGECKIGIMPGYIH+PG VG+VSRSGTLTYEAVHQ + RG+ Sbjct: 111 YMEGKKTLLVGPNCPGVITPGECKIGIMPGYIHVPGKVGIVSRSGTLTYEAVHQTSTRGL 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPVKG EFID+LK+FNEDP T+AV WI NM KPV Sbjct: 171 GQSTAVGIGGDPVKGMEFIDVLKMFNEDPDTEAVIMLGEIGGTAEEEAAEWIAANMKKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAP GKRMGHAGAIISGGKGTA+EKIA LE CGI+VA TPA +G TLV++L+E Sbjct: 231 VGFIGGQTAPEGKRMGHAGAIISGGKGTAAEKIAKLEECGIRVAKTPAVIGETLVELLKE 290 Query: 300 RGILN 304 RG+L Sbjct: 291 RGMLE 295 >Q5L0N6.1 Q5L0N6_GEOKA Succinyl-CoA ligase [ADP-forming] subunit alpha EC=6.2.1.5 Length = 300 Score = 383 bits (983), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/310 (63%), Positives = 225/310 (72%), Gaps = 11/310 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MS+ V+K+TKVI QGITGS GLFHTK ++YGT + + G+EV Sbjct: 1 MSVFVNKDTKVIVQGITGSQGLFHTKQMIEYGTNIVGGVTPG----------KGGTEVEG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +PVFD IYVPPAFAAD+IMEAVDAGL+LV+CITEGIPVLDMVKV R Sbjct: 51 VPVFDTVSEAVERTGANASVIYVPPAFAADAIMEAVDAGLDLVVCITEGIPVLDMVKVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 +MEG+ T LIGPNCPGVITP ECKIGIMPGYIH G+VG+VSRSGTLTYEAVHQLT+ GI Sbjct: 111 YMEGKKTRLIGPNCPGVITPEECKIGIMPGYIHKKGHVGIVSRSGTLTYEAVHQLTQAGI 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPV G+ FID+LK FNED T AV W+K NMTKPV Sbjct: 171 GQSTAVGIGGDPVNGTNFIDVLKAFNEDEETYAVIMIGEIGGTAEEEAAEWVKANMTKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAPPGKRMGHAGAIISGGKGTA+EKI + CGIKVA TPA +G TL+ VL+E Sbjct: 231 VGFIGGQTAPPGKRMGHAGAIISGGKGTAAEKIKKMTECGIKVAETPAVIGETLISVLKE 290 Query: 300 RGILNAFTTH 309 RG+ TH Sbjct: 291 RGLYEKCKTH 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,592,029,228 Number of extensions: 59571609 Number of successful extensions: 150486 Number of sequences better than 1.0e-05: 1610 Number of HSP's gapped: 146851 Number of HSP's successfully gapped: 1612 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)