BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0068.p (168 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIB6.1 B3KIB6_9BACI 20S proteasome A and B subunits 311 1e-83 B1DLW1.1 B1DLW1_9BACL 20S proteasome A and B subunits 277 2e-73 C0ZFA7.1 C0ZFA7_BREBN ATP-dependent protease EC=3.4.25.- 262 9e-69 Q4L5V5.1 HSLV_STAHJ ATP-dependent protease hslV EC=3.4.25.- 261 2e-68 C2LXR0.1 C2LXR0_STAHO ATP-dependent protease HslVU, peptidase su... 260 2e-68 >B3KIB6.1 B3KIB6_9BACI 20S proteasome A and B subunits Length = 180 Score = 311 bits (797), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 149/168 (88%), Positives = 161/168 (95%) Query: 1 VRHNGQAAIAGDGQVTFGESVIMKTTARKVRRLYRGQVIAGFAGSVADAITLFEKFEGKL 60 VRHNG AAIAGDGQVTFGESV+MK TA+KVRRLYRGQV+AGFAGSVADAITLFEKFEGKL Sbjct: 13 VRHNGTAAIAGDGQVTFGESVVMKQTAKKVRRLYRGQVVAGFAGSVADAITLFEKFEGKL 72 Query: 61 EEHHGNLQRAAVELAKDWRQDRILRKLEALMIVMDKEGMLLISGNGEIIEPDDDVLAIGS 120 EEHHGNLQRAAVELAKDWRQDR+LRKLEALMIVMDK GMLLISG GEIIEPDDDVLAIGS Sbjct: 73 EEHHGNLQRAAVELAKDWRQDRVLRKLEALMIVMDKTGMLLISGGGEIIEPDDDVLAIGS 132 Query: 121 GGNFALASGRALKRHASNLSAPEIAKEALQIASEICVYTNSNIIVEQL 168 GGNFAL++GRALKRHA ++ A +IA+EALQIASE+CVYTNSNIIVE+L Sbjct: 133 GGNFALSAGRALKRHAGHMEAKDIAREALQIASEVCVYTNSNIIVEEL 180 >B1DLW1.1 B1DLW1_9BACL 20S proteasome A and B subunits Length = 181 Score = 277 bits (709), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 131/168 (77%), Positives = 152/168 (90%) Query: 1 VRHNGQAAIAGDGQVTFGESVIMKTTARKVRRLYRGQVIAGFAGSVADAITLFEKFEGKL 60 VRH G+ AIAGDGQVTFG SV+MK A+KVRRLYRGQVIAGFAGSVADAITLFEKFE KL Sbjct: 13 VRHEGKGAIAGDGQVTFGNSVVMKNKAKKVRRLYRGQVIAGFAGSVADAITLFEKFEAKL 72 Query: 61 EEHHGNLQRAAVELAKDWRQDRILRKLEALMIVMDKEGMLLISGNGEIIEPDDDVLAIGS 120 EEHHGNLQRAAVELAKDWR DRILRKLEALM+VMD+ G+LLISGNGEIIEPDD +LAIGS Sbjct: 73 EEHHGNLQRAAVELAKDWRTDRILRKLEALMLVMDQSGLLLISGNGEIIEPDDGILAIGS 132 Query: 121 GGNFALASGRALKRHASNLSAPEIAKEALQIASEICVYTNSNIIVEQL 168 GGN+AL++ RAL++HA ++ A +IA+ AL+ A++ICVYTN NIIVE++ Sbjct: 133 GGNYALSAARALQKHAPHMEAKDIARAALETAADICVYTNHNIIVEEI 180 >C0ZFA7.1 C0ZFA7_BREBN ATP-dependent protease EC=3.4.25.- Length = 180 Score = 262 bits (669), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 124/168 (73%), Positives = 153/168 (91%) Query: 1 VRHNGQAAIAGDGQVTFGESVIMKTTARKVRRLYRGQVIAGFAGSVADAITLFEKFEGKL 60 V+HNG A+AGDGQVTFG S++MK A+KVRRLYRG+V+AGFAGSVADAITLFEKFEGKL Sbjct: 12 VQHNGSVAMAGDGQVTFGNSMVMKHGAKKVRRLYRGEVLAGFAGSVADAITLFEKFEGKL 71 Query: 61 EEHHGNLQRAAVELAKDWRQDRILRKLEALMIVMDKEGMLLISGNGEIIEPDDDVLAIGS 120 EE+HGNLQRAAVELAK+WR D+ILR+LEA+MIV +KE +LLISGNGEIIEPDD +LAIGS Sbjct: 72 EEYHGNLQRAAVELAKEWRMDKILRRLEAMMIVANKEHLLLISGNGEIIEPDDGILAIGS 131 Query: 121 GGNFALASGRALKRHASNLSAPEIAKEALQIASEICVYTNSNIIVEQL 168 GG+FALA+GRALK +A +L A EIA+ +L+ A+EICV+TN+N++V++L Sbjct: 132 GGSFALAAGRALKTYAPHLGAREIAEASLRTAAEICVFTNNNLVVDEL 179 >Q4L5V5.1 HSLV_STAHJ ATP-dependent protease hslV EC=3.4.25.- Length = 181 Score = 261 bits (666), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 121/168 (72%), Positives = 150/168 (89%) Query: 1 VRHNGQAAIAGDGQVTFGESVIMKTTARKVRRLYRGQVIAGFAGSVADAITLFEKFEGKL 60 VRHNG+AA+AGDGQVT GE VIMK TARKVRRLY G+V+AGFAGSVADA TLFEKFE KL Sbjct: 14 VRHNGEAAMAGDGQVTLGEQVIMKQTARKVRRLYEGKVLAGFAGSVADAFTLFEKFETKL 73 Query: 61 EEHHGNLQRAAVELAKDWRQDRILRKLEALMIVMDKEGMLLISGNGEIIEPDDDVLAIGS 120 ++ GNL+RAAVELA++WR D+ LR+LEA++IVMDK+ +L++SG GE+I PDDD++AIGS Sbjct: 74 QQFSGNLERAAVELAQEWRGDKQLRQLEAMLIVMDKDAILVVSGTGEVIAPDDDLIAIGS 133 Query: 121 GGNFALASGRALKRHASNLSAPEIAKEALQIASEICVYTNSNIIVEQL 168 GGN+AL++GRALKRHAS LSA E+A E+L++AS+ICV+TN NIIVE L Sbjct: 134 GGNYALSAGRALKRHASQLSAKEMAYESLKVASDICVFTNDNIIVETL 181 >C2LXR0.1 C2LXR0_STAHO ATP-dependent protease HslVU, peptidase subunit EC=3.4.25.- Length = 181 Score = 260 bits (665), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 120/168 (71%), Positives = 152/168 (90%) Query: 1 VRHNGQAAIAGDGQVTFGESVIMKTTARKVRRLYRGQVIAGFAGSVADAITLFEKFEGKL 60 VRHNG+AA+AGDGQVT GE VIMK TARKVRRLY G+V+AGFAGSVADA TLFEKFE KL Sbjct: 14 VRHNGEAAMAGDGQVTLGEQVIMKQTARKVRRLYEGKVLAGFAGSVADAFTLFEKFETKL 73 Query: 61 EEHHGNLQRAAVELAKDWRQDRILRKLEALMIVMDKEGMLLISGNGEIIEPDDDVLAIGS 120 ++ GNL+RAAVELA++WR D+ LR+LEA++IVMDK+G+L++SG GE+I PDDD++AIGS Sbjct: 74 QQFSGNLERAAVELAQEWRGDKQLRQLEAMLIVMDKDGILVVSGTGEVIAPDDDLIAIGS 133 Query: 121 GGNFALASGRALKRHASNLSAPEIAKEALQIASEICVYTNSNIIVEQL 168 GGN+AL++GRALKRHAS+++A E+A E+L++AS+ICV+TN NIIVE L Sbjct: 134 GGNYALSAGRALKRHASHMTAKEMAYESLKVASDICVFTNDNIIVETL 181 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 937,790,985 Number of extensions: 35383065 Number of successful extensions: 112754 Number of sequences better than 1.0e-05: 985 Number of HSP's gapped: 111053 Number of HSP's successfully gapped: 985 Length of query: 168 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 43 Effective length of database: 1,883,069,328 Effective search space: 80971981104 Effective search space used: 80971981104 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)